Biomarkers for prostate cancer and methods using the same

ABSTRACT

Methods for identifying and evaluating suites of biochemical and/or gene entities useful as biomarkers for early prediction of prostate cancer, disease grading, target identification/validation, and monitoring of drug efficacy are provided. Also provided are suites of small molecule entities as biomarkers for prostate cancer.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a divisional of U.S. Ser. No. 12/441,945, filed Feb. 12, 2010, which is a national stage application of International Application No. PCT/US2007/078805, filed Sep. 18, 2007, which claims the benefit of U.S. Provisional Application No. 60/845,600, filed Sep. 19, 2006, the entire contents of which are hereby incorporated by reference herein.

STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH

This invention was made, in part, with Government support under Grant No. U01CA111275-01 from the National Institutes of Health. The U.S. Government may have certain rights in this invention.

FIELD

The invention generally relates to biomarkers for prostate cancer and methods based on the same biomarkers.

BACKGROUND

Prostate cancer is the leading cause of male cancer-related deaths and afflicts one out of nine men over the age of 65. The American Cancer Society estimates that over 200,000 American men will be diagnosed with prostate cancer and over 30,000 will die this year. While effective surgical and radiation treatments exist for localized prostate cancer, metastatic prostate cancer remains essentially incurable and most men diagnosed with metastatic disease will succumb over a period of months to years.

Prostate cancer is detected by either a digital rectal exam (DRE), or by the measurement of levels of prostate specific antigen (PSA), which has an unacceptably high rate of false-positives. The diagnosis of prostate cancer can be confirmed only by a biopsy. Radical prostatectomy, radiation and watchful waiting are generally effective for localized prostate cancer, but it is often difficult to determine which approach to use. Since it is not possible to distinguish between the indolent and more aggressive tumors current therapy takes a very conservative approach.

While imaging, X-rays, computerized tomography scans and further biopsies can help determine if prostate cancer has metastasized, they are not able to differentiate early stages. Understanding the progression of prostate cancer from a localized, early, indolent state, to an aggressive state, and, ultimately, to a metastatic state would allow the proper clinical management of this disease. Furthermore, early-indolent prostate cancer may be progressive or non-progressive toward aggressive forms.

SUMMARY

In one aspect, the present invention provides a method of diagnosing whether a subject has prostate cancer, comprising analyzing a biological sample from a subject to determine the level(s) of one or more biomarkers for prostate cancer in the sample, where the one or more biomarkers are selected from Tables 1, 2, 4, 5, 6, 7, 9, 10, 13, 15, 18, 22, and/or 24 and comparing the level(s) of the one or more biomarkers in the sample to prostate cancer-positive and/or prostate cancer-negative reference levels of the one or more biomarkers in order to diagnose whether the subject has prostate cancer.

In another aspect, the present invention also provides a method of determining whether a subject is predisposed to developing prostate cancer, comprising analyzing a biological sample from a subject to determine the level(s) of one or more biomarkers for prostate cancer in the sample, where the one or more biomarkers are selected from Tables 1, 2, 4, 5, 6, 7, 9, 10, 13, 15, 18, 20, 22, 24, and/or 26; and comparing the level(s) of the one or more biomarkers in the sample to prostate cancer-positive and/or prostate cancer-negative reference levels of the one or more biomarkers in order to determine whether the subject is predisposed to developing prostate cancer.

In yet another aspect, the invention provides a method of monitoring progression/regression of prostate cancer in a subject comprising analyzing a first biological sample from a subject to determine the level(s) of one or more biomarkers for prostate cancer in the sample, where the one or more biomarkers are selected from Tables 1, 2, 3, 4, 5, 6, 7, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26 and the first sample is obtained from the subject at a first time point; analyzing a second biological sample from a subject to determine the level(s) of the one or more biomarkers, where the second sample is obtained from the subject at a second time point; and comparing the level(s) of one or more biomarkers in the first sample to the level(s) of the one or more biomarkers in the second sample in order to monitor the progression/regression of prostate cancer in the subject.

In another aspect, the present invention provides a method of assessing the efficacy of a composition for treating prostate cancer comprising analyzing, from a subject having prostate cancer and currently or previously being treated with a composition, a biological sample to determine the level(s) of one or more biomarkers for prostate cancer selected from Tables 1, 2, 4, 5, 6, 7, 9, 10, 13, 15, 18, 20, 22, 24, and/or 26; and comparing the level(s) of the one or more biomarkers in the sample to (a) levels of the one or more biomarkers in a previously-taken biological sample from the subject, where the previously-taken biological sample was obtained from the subject before being treated with the composition, (b) prostate cancer-positive reference levels of the one or more biomarkers, and/or (c) prostate cancer-negative reference levels of the one or more biomarkers.

In another aspect, the present invention provides a method for assessing the efficacy of a composition in treating prostate cancer, comprising analyzing a first biological sample from a subject to determine the level(s) of one or more biomarkers for prostate cancer selected from Tables 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26, the first sample obtained from the subject at a first time point; administering the composition to the subject; analyzing a second biological sample from the subject to determine the level(s) of the one or more biomarkers, the second sample obtained from the subject at a second time point after administration of the composition; comparing the level(s) of one or more biomarkers in the first sample to the level(s) of the one or more biomarkers in the second sample in order to assess the efficacy of the composition for treating prostate cancer.

In yet another aspect, the invention provides a method of assessing the relative efficacy of two or more compositions for treating prostate cancer comprising analyzing, from a first subject having prostate cancer and currently or previously being treated with a first composition, a first biological sample to determine the level(s) of one or more biomarkers selected from Tables 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26; analyzing, from a second subject having prostate cancer and currently or previously being treated with a second composition, a second biological sample to determine the level(s) of the one or more biomarkers; and comparing the level(s) of one or more biomarkers in the first sample to the level(s) of the one or more biomarkers in the second sample in order to assess the relative efficacy of the first and second compositions for treating prostate cancer.

In another aspect, the present invention provides a method for screening a composition for activity in modulating one or more biomarkers of prostate cancer, comprising contacting one or more cells with a composition; analyzing at least a portion of the one or more cells or a biological sample associated with the cells to determine the level(s) of one or more biomarkers of prostate cancer selected from Tables 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26; and comparing the level(s) of the one or more biomarkers with predetermined standard levels for the biomarkers to determine whether the composition modulated the level(s) of the one or more biomarkers.

In a further aspect, the present invention provides a method for identifying a potential drug target for prostate cancer comprising identifying one or more biochemical pathways associated with one or more biomarkers for prostate cancer selected from Tables 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26; and identifying a protein affecting at least one of the one or more identified biochemical pathways, the protein being a potential drug target for prostate cancer.

In yet another aspect, the invention provides a method for treating a subject having prostate cancer comprising administering to the subject an effective amount of one or more biomarkers selected from Tables 1, 2, 4, 5, 6, 7, 9, 10, 13, 15, 18, 20, 22, 24, and/or 26 that are decreased in prostate cancer.

In another aspect, the invention also provides a method of distinguishing low grade prostate cancer from high grade prostate cancer in a subject having prostate cancer, comprising analyzing a biological sample from a subject to determine the level(s) of one or more biomarkers for low grade prostate cancer and/or high grade prostate cancer in the sample, where the one or more biomarkers are selected from Tables 3, 8, 11, 20 and/or 26 and comparing the level(s) of the one or more biomarkers in the sample to low grade prostate cancer-positive reference levels that distinguish over high grade prostate cancer and/or to high grade prostate cancer-positive reference levels that distinguish over low grade prostate cancer in order to determine whether the subject has low grade or high grade prostate cancer.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 provides an importance plot of one example of metabolites to distinguish Normal (N), Localized cancer tumor (T), and Metastatic tumor (M) tissue types.

FIG. 2 provides an importance plot of one example of metabolites to distinguish Normal prostate tissue (N) and Localized prostate tumor tissue (T).

FIG. 3 provides an importance plot of one example of metabolites to distinguish Non-cancer tissue (Control) and lower grade prostate cancer tissue (PCA) using urine samples.

FIG. 4 provides an importance plot of one example of metabolites to distinguish lower grade prostate cancer tissues and higher grade prostate cancer tissues from urine samples.

FIG. 5 provides an importance plot of one example of metabolites to distinguish non-cancer tissue (Control) and lower grade prostate cancer tissue (PCA) using plasma samples.

FIG. 6 provides an importance plot of one example of metabolites to distinguish lower grade prostate cancer tissues and higher grade prostate cancer tissues using plasma samples.

FIG. 7 provides an importance plot of one example of metabolites to distinguish subjects with lower grade prostate cancer and higher grade prostate cancer.

DETAILED DESCRIPTION

The present invention relates to biomarkers of prostate cancer, methods for diagnosis of prostate cancer, methods of distinguishing between low grade and high grade prostate cancer, methods of determining predisposition to prostate cancer, methods of monitoring progression/regression of prostate cancer, methods of assessing efficacy of compositions for treating prostate cancer, methods of screening compositions for activity in modulating biomarkers of prostate cancer, methods of treating prostate cancer, as well as other methods based on biomarkers of prostate cancer. Prior to describing this invention in further detail, however, the following terms will first be defined.

DEFINITIONS

“Biomarker” means a compound, preferably a metabolite, that is differentially present (i.e., increased or decreased) in a biological sample from a subject or a group of subjects having a first phenotype (e.g., having a disease) as compared to a biological sample from a subject or group of subjects having a second phenotype (e.g., not having the disease). A biomarker may be differentially present at any level, but is generally present at a level that is increased by at least 5%, by at least 10%, by at least 15%, by at least 20%, by at least 25%, by at least 30%, by at least 35%, by at least 40%, by at least 45%, by at least 50%, by at least 55%, by at least 60%, by at least 65%, by at least 70%, by at least 75%, by at least 80%, by at least 85%, by at least 90%, by at least 95%, by at least 100%, by at least 110%, by at least 120%, by at least 130%, by at least 140%, by at least 150%, or more; or is generally present at a level that is decreased by at least 5%, by at least 10%, by at least 15%, by at least 20%, by at least 25%, by at least 30%, by at least 35%, by at least 40%, by at least 45%, by at least 50%, by at least 55%, by at least 60%, by at least 65%, by at least 70%, by at least 75%, by at least 80%, by at least 85%, by at least 90%, by at least 95%, or by 100% (i.e., absent). A biomarker is preferably differentially present at a level that is statistically significant (i.e., a p-value less than 0.05 and/or a q-value of less than 0.10 as determined using either Welch's T-test or Wilcoxon's rank-sum Test).

The “level” of one or more biomarkers means the absolute or relative amount or concentration of the biomarker in the sample.

“Sample” or “biological sample” means biological material isolated from a subject. The biological sample may contain any biological material suitable for detecting the desired biomarkers, and may comprise cellular and/or non-cellular material from the subject. The sample can be isolated from any suitable biological tissue or fluid such as, for example, prostate tissue, blood, blood plasma, urine, or cerebral spinal fluid (CSF).

“Subject” means any animal, but is preferably a mammal, such as, for example, a human, monkey, mouse, or rabbit.

A “reference level” of a biomarker means a level of the biomarker that is indicative of a particular disease state, phenotype, or lack thereof, as well as combinations of disease states, phenotypes, or lack thereof. A “positive” reference level of a biomarker means a level that is indicative of a particular disease state or phenotype. A “negative” reference level of a biomarker means a level that is indicative of a lack of a particular disease state or phenotype. For example, a “prostate cancer-positive reference level” of a biomarker means a level of a biomarker that is indicative of a positive diagnosis of prostate cancer in a subject, and a “prostate cancer-negative reference level” of a biomarker means a level of a biomarker that is indicative of a negative diagnosis of prostate cancer in a subject. A “reference level” of a biomarker may be an absolute or relative amount or concentration of the biomarker, a presence or absence of the biomarker, a range of amount or concentration of the biomarker, a minimum and/or maximum amount or concentration of the biomarker, a mean amount or concentration of the biomarker, and/or a median amount or concentration of the biomarker; and, in addition, “reference levels” of combinations of biomarkers may also be ratios of absolute or relative amounts or concentrations of two or more biomarkers with respect to each other. Appropriate positive and negative reference levels of biomarkers for a particular disease state, phenotype, or lack thereof may be determined by measuring levels of desired biomarkers in one or more appropriate subjects, and such reference levels may be tailored to specific populations of subjects (e.g., a reference level may be age-matched so that comparisons may be made between biomarker levels in samples from subjects of a certain age and reference levels for a particular disease state, phenotype, or lack thereof in a certain age group). Such reference levels may also be tailored to specific techniques that are used to measure levels of biomarkers in biological samples (e.g., LC-MS, GC-MS, etc.), where the levels of biomarkers may differ based on the specific technique that is used.

“Non-biomarker compound” means a compound that is not differentially present in a biological sample from a subject or a group of subjects having a first phenotype (e.g., having a first disease) as compared to a biological sample from a subject or group of subjects having a second phenotype (e.g., not having the first disease). Such non-biomarker compounds may, however, be biomarkers in a biological sample from a subject or a group of subjects having a third phenotype (e.g., having a second disease) as compared to the first phenotype (e.g., having the first disease) or the second phenotype (e.g., not having the first disease).

“Metabolite”, or “small molecule”, means organic and inorganic molecules which are present in a cell. The term does not include large macromolecules, such as large proteins (e.g., proteins with molecular weights over 2,000, 3,000, 4,000, 5,000, 6,000, 7,000, 8,000, 9,000, or 10,000), large nucleic acids (e.g., nucleic acids with molecular weights of over 2,000, 3,000, 4,000, 5,000, 6,000, 7,000, 8,000, 9,000, or 10,000), or large polysaccharides (e.g., polysaccharides with a molecular weights of over 2,000, 3,000, 4,000, 5,000, 6,000, 7,000, 8,000, 9,000, or 10,000). The small molecules of the cell are generally found free in solution in the cytoplasm or in other organelles, such as the mitochondria, where they form a pool of intermediates which can be metabolized further or used to generate large molecules, called macromolecules. The term “small molecules” includes signaling molecules and intermediates in the chemical reactions that transform energy derived from food into usable forms. Examples of small molecules include sugars, fatty acids, amino acids, nucleotides, intermediates formed during cellular processes, and other small molecules found within the cell.

“Metabolic profile”, or “small molecule profile”, means a complete or partial inventory of small molecules within a targeted cell, tissue, organ, organism, or fraction thereof (e.g., cellular compartment). The inventory may include the quantity and/or type of small molecules present. The “small molecule profile” may be determined using a single technique or multiple different techniques.

“Metabolome” means all of the small molecules present in a given organism.

“Prostate cancer” refers to a disease in which cancer develops in the prostate, a gland in the male reproductive system. “Low grade” or “lower grade” prostate cancer refers to non-metastatic prostate cancer, including malignant tumors with low potential for metastisis (i.e. prostate cancer that is considered to be less aggressive). “High grade” or “higher grade” prostate cancer refers to prostate cancer that has metastasized in a subject, including malignant tumors with high potential for metastisis (prostate cancer that is considered to be aggressive).

I. Biomarkers

The prostate cancer biomarkers described herein were discovered using metabolomic profiling techniques. Such metabolomic profiling techniques are described in more detail in the Examples set forth below as well as in U.S. Pat. No. 7,005,255 and U.S. patent application Ser. Nos. 11/357,732, 10/695,265 (Publication No. 2005/0014132), 11/301,077 (Publication No. 2006/0134676), 11/301,078 (Publication No. 2006/0134677), 11/301,079 (Publication No. 2006/0134678), and 11/405,033, the entire contents of which are hereby incorporated herein by reference.

Generally, metabolic profiles were determined for biological samples from human subjects diagnosed with prostate cancer as well as from one or more other groups of human subjects (e.g., healthy control subjects not diagnosed with prostate cancer), as well as from human subjects diagnosed with lower grade prostate cancer and human subjects diagnosed with metastatic/high grade prostate cancer. The metabolic profile for biological samples from a subject having prostate cancer was compared to the metabolic profile for biological samples from the one or more other groups of subjects. Those molecules differentially present, including those molecules differentially present at a level that is statistically significant, in the metabolic profile of samples from subjects with prostate cancer as compared to another group (e.g., healthy control subjects not diagnosed with prostate cancer) were identified as biomarkers to distinguish those groups. In addition, those molecules differentially present, including those molecules differentially present at a level that is statistically significant, in the metabolic profile of samples from subjects with low grade prostate cancer as compared to high grade prostate cancer were also identified as biomarkers to distinguish those groups.

The biomarkers are discussed in more detail herein. The biomarkers that were discovered correspond with the following group(s):

-   -   Biomarkers for distinguishing subjects having prostate cancer         vs. control subjects not diagnosed with prostate cancer (see         Tables 1, 2, 4, 5, 6, 7, 9, 10, 15, 18, 22, 24);     -   Biomarkers for distinguishing subjects having low grade prostate         cancer vs. control subjects not diagnosed with prostate cancer         (see Tables 1, 6, 9, 22);     -   Biomarkers for distinguishing subjects having metastatic/high         grade prostate cancer vs. control subjects not diagnosed with         prostate cancer (see Tables 2, 7, 10, 24);     -   Biomarkers for distinguishing subjects having metastatic/high         grade prostate cancer vs. subjects having low grade prostate         cancer (see Tables 3, 8, 11, 20, 26).

Although the identities of some of the biomarkers compounds are not known at this time, such identities are not necessary for the identification of the biomarkers in biological samples from subjects, as the “unnamed” compounds have been sufficiently characterized by analytical techniques to allow such identification. The analytical characterization of all such “unnamed” compounds is listed in the Examples. Such “unnamed” biomarkers are designated herein using the nomenclature “Metabolite” followed by a specific metabolite number.

IIA. Diagnosis of Prostate Cancer

The identification of biomarkers for prostate cancer allows for the diagnosis of (or for aiding in the diagnosis of) prostate cancer in subjects presenting one or more symptoms of prostate cancer. A method of diagnosing (or aiding in diagnosing) whether a subject has prostate cancer comprises (1) analyzing a biological sample from a subject to determine the level(s) of one or more biomarkers of prostate cancer in the sample and (2) comparing the level(s) of the one or more biomarkers in the sample to prostate cancer-positive and/or prostate cancer-negative reference levels of the one or more biomarkers in order to diagnose (or aid in the diagnosis of) whether the subject has prostate cancer. The one or more biomarkers that are used are selected from Tables 1, 2, 4, 5, 6, 7, 9, 10, 13, 15, 18, 22, and/or 24 and combinations thereof. When such a method is used to aid in the diagnosis of prostate cancer, the results of the method may be used along with other methods (or the results thereof) useful in the clinical determination of whether a subject has prostate cancer.

Any suitable method may be used to analyze the biological sample in order to determine the level(s) of the one or more biomarkers in the sample. Suitable methods include chromatography (e.g., HPLC, gas chromatography, liquid chromatography), mass spectrometry (e.g., MS, MS-MS), enzyme-linked immunosorbent assay (ELISA), antibody linkage, other immunochemical techniques, and combinations thereof. Further, the level(s) of the one or more biomarkers may be measured indirectly, for example, by using an assay that measures the level of a compound (or compounds) that correlates with the level of the biomarker(s) that are desired to be measured.

The levels of one or more of the biomarkers of Tables 1, 2, 4, 5, 6, 7, 9, 10, 13, 15, 18, 22, and/or 24 may be determined in the methods of diagnosing and methods of aiding in diagnosing whether a subject has prostate cancer. For example, the level(s) of one biomarker, two or more biomarkers, three or more biomarkers, four or more biomarkers, five or more biomarkers, six or more biomarkers, seven or more biomarkers, eight or more biomarkers, nine or more biomarkers, ten or more biomarkers, etc., including a combination of all of the biomarkers in Tables 1, 2, 4, 5, 6, 7, 9, 10, 13, 15, 18, 22, and/or 24 or any fraction thereof, may be determined and used in such methods. Determining levels of combinations of the biomarkers may allow greater sensitivity and specificity in diagnosing prostate cancer and aiding in the diagnosis of prostate cancer, and may allow better differentiation of prostate cancer from other prostate disorders (e.g. benign prostatic hypertrophy (BPH), prostatitis, etc.) or other cancers that may have similar or overlapping biomarkers to prostate cancer (as compared to a subject not having prostate cancer). For example, ratios of the levels of certain biomarkers (and non-biomarker compounds) in biological samples may allow greater sensitivity and specificity in diagnosing prostate cancer and aiding in the diagnosis of prostate cancer and may allow better differentiation of prostate cancer from other cancers or other disorders of the prostate that may have similar or overlapping biomarkers to prostate cancer (as compared to a subject not having prostate cancer).

One or more biomarkers that are specific for diagnosing prostate cancer (or aiding in diagnosing prostate cancer) in a certain type of sample (e.g., prostate tissue sample, urine sample, or blood plasma sample) may also be used. For example, when the biological sample is prostate tissue, one or more biomarkers listed in Tables 1, 2, 13, and/or 15, may be used to diagnose (or aid in diagnosing) whether a subject has prostate cancer. When the biological sample is blood plasma, one or more biomarkers listed in Tables 4, 6, 7, 22, and/or 24 may be used to diagnose (or aid in diagnosing) whether a subject has prostate cancer. When the biological sample is urine, one or more biomarkers listed in Tables 5, 9, 10, and/or 18 may be used to diagnose (or aid in diagnosing) whether a subject has prostate cancer.

After the level(s) of the one or more biomarkers in the sample are determined, the level(s) are compared to prostate cancer-positive and/or prostate cancer-negative reference levels to aid in diagnosing or to diagnose whether the subject has prostate cancer. Levels of the one or more biomarkers in a sample matching the prostate cancer-positive reference levels (e.g., levels that are the same as the reference levels, substantially the same as the reference levels, above and/or below the minimum and/or maximum of the reference levels, and/or within the range of the reference levels) are indicative of a diagnosis of prostate cancer in the subject. Levels of the one or more biomarkers in a sample matching the prostate cancer-negative reference levels (e.g., levels that are the same as the reference levels, substantially the same as the reference levels, above and/or below the minimum and/or maximum of the reference levels, and/or within the range of the reference levels) are indicative of a diagnosis of no prostate cancer in the subject. In addition, levels of the one or more biomarkers that are differentially present (especially at a level that is statistically significant) in the sample as compared to prostate cancer-negative reference levels are indicative of a diagnosis of prostate cancer in the subject. Levels of the one or more biomarkers that are differentially present (especially at a level that is statistically significant) in the sample as compared to prostate cancer-positive reference levels are indicative of a diagnosis of no prostate cancer in the subject.

The level(s) of the one or more biomarkers may be compared to prostate cancer-positive and/or prostate cancer-negative reference levels using various techniques, including a simple comparison (e.g., a manual comparison) of the level(s) of the one or more biomarkers in the biological sample to prostate cancer-positive and/or prostate cancer-negative reference levels. The level(s) of the one or more biomarkers in the biological sample may also be compared to prostate cancer-positive and/or prostate cancer-negative reference levels using one or more statistical analyses (e.g., t-test, Welch's T-test, Wilcoxon's rank sum test, random forest).

In addition, the biological samples may be analyzed to determine the level(s) of one or more non-biomarker compounds. The level(s) of such non-biomarker compounds may also allow differentiation of prostate cancer from other prostate disorders that may have similar or overlapping biomarkers to prostate cancer (as compared to a subject not having a prostate disorder). For example, a known non-biomarker compound present in biological samples of subjects having prostate cancer and subjects not having prostate cancer could be monitored to verify a diagnosis of prostate cancer as compared to a diagnosis of another prostate disorder when biological samples from subjects having the prostate disorder do not have the non-biomarker compound.

The methods of diagnosing (or aiding in diagnosing) whether a subject has prostate cancer may also be conducted specifically to diagnose (or aid in diagnosing) whether a subject has low grade prostate cancer and/or high grade prostate cancer. Such methods comprise (1) analyzing a biological sample from a subject to determine the level(s) of one or more biomarkers of low grade prostate cancer (and/or high grade prostate cancer) in the sample and (2) comparing the level(s) of the one or more biomarkers in the sample to low grade prostate cancer-positive and/or low grade prostate cancer-negative reference levels (or high grade prostate cancer-positive and/or high grade prostate cancer-negative reference levels) in order to diagnose (or aid in the diagnosis of) whether the subject has low grade prostate cancer (or high grade prostate cancer). Biomarker specific for low grade prostate cancer are listed in Tables 1, 6, 9, 22 and biomarkers specific for high grade prostate cancer are listed in Tables 2, 7, 10, 24.

IIB. Methods of Distinguishing Low Grade Prostate Cancer from High Grade Prostate Cancer

The identification of biomarkers for distinguishing low grade prostate cancer versus high grade prostate cancer allows low grade prostate cancer and high grade prostate cancer to be distinguished in patients. A method of distinguishing low grade prostate cancer from high grade prostate cancer in a subject having prostate cancer comprises (1) analyzing a biological sample from a subject to determine the level(s) in the sample of one or more biomarkers of low grade prostate cancer that distinguish over high grade prostate cancer and/or one or more biomarkers of high grade prostate cancer that distinguish over low grade prostate cancer, and (2) comparing the level(s) of the one or more biomarkers in the sample to low grade prostate cancer-positive reference levels that distinguish over high grade prostate cancer and/or high grade prostate cancer-positive reference levels that distinguish over low grade prostate cancer of the one or more biomarkers in order to determine whether the subject has low grade or high grade prostate cancer. The one or more biomarkers that are used are selected from Tables 3, 8, 11, 20, and/or 26 and combinations thereof.

Any suitable method may be used to analyze the biological sample in order to determine the level(s) of the one or more biomarkers in the sample. Suitable methods include chromatography (e.g., HPLC, gas chromatography, liquid chromatography), mass spectrometry (e.g., MS, MS-MS), enzyme-linked immunosorbent assay (ELISA), antibody linkage, other immunochemical techniques, and combinations thereof. Further, the level(s) of the one or more biomarkers may be measured indirectly, for example, by using an assay that measures the level of a compound (or compounds) that correlates with the level of the biomarker(s) that are desired to be measured.

The levels of one or more of the biomarkers of Tables 3, 8, 11, 20, and/or 26 may be determined in the methods of diagnosing and methods of aiding in diagnosing whether a subject has prostate cancer. For example, the level(s) of one biomarker, two or more biomarkers, three or more biomarkers, four or more biomarkers, five or more biomarkers, six or more biomarkers, seven or more biomarkers, eight or more biomarkers, nine or more biomarkers, ten or more biomarkers, etc., including a combination of all of the biomarkers in Tables 3, 8, 11, 20, and/or 26 or any fraction thereof, may be determined and used in such methods. Determining levels of combinations of the biomarkers may allow greater sensitivity and specificity in distinguishing between low grade and high grade prostate cancer.

One or more biomarkers that are specific for distinguishing between low grade and high grade prostate cancer in a certain type of sample (e.g., prostate tissue sample, urine sample, or blood plasma sample) may also be used. For example, when the biological sample is prostate tissue, one or more biomarkers listed in Table 3 may be used. When the biological sample is blood plasma, one or more biomarkers listed in Table 8 or 26 may be used. When the biological sample is urine, one or more biomarkers listed in Table 11 or 20 may be used.

After the level(s) of the one or more biomarkers in the sample are determined, the level(s) are compared to low grade prostate cancer-positive reference levels that distinguish over high grade prostate cancer-negative and/or high grade prostate cancer-positive reference levels that distinguish over low grade prostate cancer of the one or more biomarkers in order to determine whether the subject has low grade or high grade prostate cancer. Levels of the one or more biomarkers in a sample matching the low grade prostate cancer-positive reference levels that distinguish over high grade prostate cancer (e.g., levels that are the same as the reference levels, substantially the same as the reference levels, above and/or below the minimum and/or maximum of the reference levels, and/or within the range of the reference levels) are indicative of low-grade prostate cancer in the subject. Levels of the one or more biomarkers in a sample matching the high grade prostate cancer-positive reference levels that distinguish over low grade prostate cancer (e.g., levels that are the same as the reference levels, substantially the same as the reference levels, above and/or below the minimum and/or maximum of the reference levels, and/or within the range of the reference levels) are indicative of high-grade prostate cancer in the subject. If the level(s) of the one or more biomarkers are more similar to the low grade prostate cancer-positive reference levels that distinguish over high grade prostate cancer (or less similar to the high grade prostate cancer-positive reference levels), then the results are indicative of low grade prostate cancer in the subject. If the level(s) of the one or more biomarkers are more similar to the high grade prostate cancer-positive reference levels that distinguish over low grade prostate cancer (or less similar to the low grade prostate cancer-positive reference levels), then the results are indicative of high grade prostate cancer in the subject.

The level(s) of the one or more biomarkers may be compared to low grade prostate cancer-positive reference levels that distinguish over high grade prostate cancer and/or high grade prostate cancer-positive reference levels that distinguish over low grade prostate cancer using various techniques, including a simple comparison (e.g., a manual comparison) of the level(s) of the one or more biomarkers in the biological sample to low grade prostate cancer-positive and/or high grade prostate cancer-positive reference levels. The level(s) of the one or more biomarkers in the biological sample may also be compared to low grade prostate cancer-positive reference levels that distinguish over high grade prostate cancer and/or high grade prostate cancer-positive reference levels that distinguish over low grade prostate cancer using one or more statistical analyses (e.g., t-test, Welch's T-test, Wilcoxon's rank sum test, random forest).

In addition, the biological samples may be analyzed to determine the level(s) of one or more non-biomarker compounds. The level(s) of such non-biomarker compounds may also allow differentiation of low grade prostate cancer from high grade prostate cancer.

III. Methods of Determining Predisposition to Prostate Cancer

The identification of biomarkers for prostate cancer also allows for the determination of whether a subject having no symptoms of prostate cancer is predisposed to developing prostate cancer. A method of determining whether a subject having no symptoms of prostate cancer is predisposed to developing prostate cancer comprises (1) analyzing a biological sample from a subject to determine the level(s) of one or more biomarkers listed in Tables 1, 2, 4, 5, 6, 7, 9, 10, 13, 15, 18, 22, and/or 24 in the sample and (2) comparing the level(s) of the one or more biomarkers in the sample to prostate cancer-positive and/or prostate cancer-negative reference levels of the one or more biomarkers in order to determine whether the subject is predisposed to developing prostate cancer. The results of the method may be used along with other methods (or the results thereof) useful in the clinical determination of whether a subject is predisposed to developing prostate cancer.

As described above in connection with methods of diagnosing (or aiding in the diagnosis of) prostate cancer, any suitable method may be used to analyze the biological sample in order to determine the level(s) of the one or more biomarkers in the sample.

As with the methods of diagnosing (or aiding in the diagnosis of) prostate cancer described above, the level(s) of one biomarker, two or more biomarkers, three or more biomarkers, four or more biomarkers, five or more biomarkers, six or more biomarkers, seven or more biomarkers, eight or more biomarkers, nine or more biomarkers, ten or more biomarkers, etc., including a combination of all of the biomarkers in Tables 1, 2, 4, 5, 6, 7, 9, 10, 13, 15, 18, 22, and/or 24 or any fraction thereof, may be determined and used in methods of determining whether a subject having no symptoms of prostate cancer is predisposed to developing prostate cancer.

After the level(s) of the one or more biomarkers in the sample are determined, the level(s) are compared to prostate cancer-positive and/or prostate cancer-negative reference levels in order to predict whether the subject is predisposed to developing prostate cancer. Levels of the one or more biomarkers in a sample matching the prostate cancer-positive reference levels (e.g., levels that are the same as the reference levels, substantially the same as the reference levels, above and/or below the minimum and/or maximum of the reference levels, and/or within the range of the reference levels) are indicative of the subject being predisposed to developing prostate cancer. Levels of the one or more biomarkers in a sample matching the prostate cancer-negative reference levels (e.g., levels that are the same as the reference levels, substantially the same as the reference levels, above and/or below the minimum and/or maximum of the reference levels, and/or within the range of the reference levels) are indicative of the subject not being predisposed to developing prostate cancer. In addition, levels of the one or more biomarkers that are differentially present (especially at a level that is statistically significant) in the sample as compared to prostate cancer-negative reference levels are indicative of the subject being predisposed to developing prostate cancer. Levels of the one or more biomarkers that are differentially present (especially at a level that is statistically significant) in the sample as compared to prostate cancer-positive reference levels are indicative of the subject not being predisposed to developing prostate cancer.

Furthermore, it may also be possible to determine reference levels specific to assessing whether or not a subject that does not have prostate cancer is predisposed to developing prostate cancer. For example, it may be possible to determine reference levels of the biomarkers for assessing different degrees of risk (e.g., low, medium, high) in a subject for developing prostate cancer. Such reference levels could be used for comparison to the levels of the one or more biomarkers in a biological sample from a subject.

As with the methods described above, the level(s) of the one or more biomarkers may be compared to prostate cancer-positive and/or prostate cancer-negative reference levels using various techniques, including a simple comparison, one or more statistical analyses, and combinations thereof.

As with the methods of diagnosing (or aiding in diagnosing) whether a subject has prostate cancer, the methods of determining whether a subject having no symptoms of prostate cancer is predisposed to developing prostate cancer may further comprise analyzing the biological sample to determine the level(s) of one or more non-biomarker compounds.

The methods of determining whether a subject having no symptoms of prostate cancer is predisposed to developing prostate cancer may also be conducted specifically to determine whether a subject having no symptoms of prostate cancer is predisposed to developing low grade prostate cancer and/or high grade prostate cancer. Biomarker specific for low grade prostate cancer are listed in Tables 1, 6, 9, and 22 and biomarkers specific for high grade prostate cancer are listed in Tables 2, 7, 10, and 24.

In addition, methods of determining whether a subject having low grade prostate cancer is predisposed to developing high grade prostate cancer may be conducted using one or more biomarkers selected from Tables 3, 8, 11, 20, and 26.

IV. Methods of Monitoring Progression/Regression of Prostate Cancer

The identification of biomarkers for prostate cancer also allows for monitoring progression/regression of prostate cancer in a subject. A method of monitoring the progression/regression of prostate cancer in a subject comprises (1) analyzing a first biological sample from a subject to determine the level(s) of one or more biomarkers for prostate cancer selected from Tables 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26, the first sample obtained from the subject at a first time point, (2) analyzing a second biological sample from a subject to determine the level(s) of the one or more biomarkers, the second sample obtained from the subject at a second time point, and (3) comparing the level(s) of one or more biomarkers in the first sample to the level(s) of the one or more biomarkers in the second sample in order to monitor the progression/regression of prostate cancer in the subject. The results of the method are indicative of the course of prostate cancer (i.e., progression or regression, if any change) in the subject.

The change (if any) in the level(s) of the one or more biomarkers over time may be indicative of progression or regression of prostate cancer in the subject. In order to characterize the course of prostate cancer in the subject, the level(s) of the one or more biomarkers in the first sample, the level(s) of the one or more biomarkers in the second sample, and/or the results of the comparison of the levels of the biomarkers in the first and second samples may be compared to prostate cancer-positive, prostate cancer-negative, low grade prostate cancer-positive, low grade prostate cancer-negative, high-grade prostate cancer-positive, and/or high grade prostate cancer-negative reference levels as well as low grade prostate cancer-positive reference levels that distinguish over high grade prostate cancer and/or high grade prostate cancer-positive reference levels that distinguish over low grade prostate cancer. If the comparisons indicate that the level(s) of the one or more biomarkers are increasing or decreasing over time (e.g., in the second sample as compared to the first sample) to become more similar to the prostate cancer-positive reference levels (or less similar to the prostate cancer-negative reference levels), to the high grade prostate cancer reference levels, or, when the subject initially has low grade prostate cancer, to the high grade prostate cancer-positive reference levels that distinguish over low grade prostate cancer, then the results are indicative of prostate cancer progression. If the comparisons indicate that the level(s) of the one or more biomarkers are increasing or decreasing over time to become more similar to the prostate cancer-negative reference levels (or less similar to the prostate cancer-positive reference levels), or, when the subject initially has high grade prostate cancer, to low grade prostate cancer reference levels and/or to low grade prostate cancer-positive reference levels that distinguish over high grade prostate cancer, then the results are indicative of prostate cancer regression.

As with the other methods described herein, the comparisons made in the methods of monitoring progression/regression of prostate cancer in a subject may be carried out using various techniques, including simple comparisons, one or more statistical analyses, and combinations thereof.

The results of the method may be used along with other methods (or the results thereof) useful in the clinical monitoring of progression/regression of prostate cancer in a subject.

As described above in connection with methods of diagnosing (or aiding in the diagnosis of) prostate cancer, any suitable method may be used to analyze the biological samples in order to determine the level(s) of the one or more biomarkers in the samples. In addition, the level(s) one or more biomarkers, including a combination of all of the biomarkers in Tables 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26 or any fraction thereof, may be determined and used in methods of monitoring progression/regression of prostate cancer in a subject.

Such methods could be conducted to monitor the course of prostate cancer in subjects having prostate cancer or could be used in subjects not having prostate cancer (e.g., subjects suspected of being predisposed to developing prostate cancer) in order to monitor levels of predisposition to prostate cancer.

V. Methods of Assessing Efficacy of Compositions for Treating Prostate Cancer

The identification of biomarkers for prostate cancer also allows for assessment of the efficacy of a composition for treating prostate cancer as well as the assessment of the relative efficacy of two or more compositions for treating prostate cancer. Such assessments may be used, for example, in efficacy studies as well as in lead selection of compositions for treating prostate cancer.

A method of assessing the efficacy of a composition for treating prostate cancer comprises (1) analyzing, from a subject having prostate cancer and currently or previously being treated with a composition, a biological sample to determine the level(s) of one or more biomarkers selected from Tables 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26, and (2) comparing the level(s) of the one or more biomarkers in the sample to (a) level(s) of the one or more biomarkers in a previously-taken biological sample from the subject, wherein the previously-taken biological sample was obtained from the subject before being treated with the composition, (b) prostate cancer-positive reference levels (including low grade prostate cancer-positive and/or high grade prostate cancer-positive reference levels) of the one or more biomarkers, (c) prostate cancer-negative reference levels (including low grade prostate cancer-negative and/or high grade prostate cancer-negative reference levels) of the one or more biomarkers, (d) low grade prostate cancer-positive reference levels that distinguish over high grade prostate cancer, and/or (e) high grade prostate cancer-positive reference levels that distinguish over low grade prostate cancer. The results of the comparison are indicative of the efficacy of the composition for treating prostate cancer.

Thus, in order to characterize the efficacy of the composition for treating prostate cancer, the level(s) of the one or more biomarkers in the biological sample are compared to (1) prostate cancer-positive reference levels, (2) prostate cancer-negative reference levels, (3) previous levels of the one or more biomarkers in the subject before treatment with the composition, (4) low grade prostate cancer-positive reference levels that distinguish over high grade prostate cancer, and/or (5) high grade prostate cancer-positive reference levels that distinguish over low grade prostate cancer.

When comparing the level(s) of the one or more biomarkers in the biological sample (from a subject having prostate cancer and currently or previously being treated with a composition) to prostate cancer-positive reference levels and/or prostate cancer-negative reference levels, level(s) in the sample matching the prostate cancer-negative reference levels (e.g., levels that are the same as the reference levels, substantially the same as the reference levels, above and/or below the minimum and/or maximum of the reference levels, and/or within the range of the reference levels) are indicative of the composition having efficacy for treating prostate cancer. Levels of the one or more biomarkers in the sample matching the prostate cancer-positive reference levels (e.g., levels that are the same as the reference levels, substantially the same as the reference levels, above and/or below the minimum and/or maximum of the reference levels, and/or within the range of the reference levels) are indicative of the composition not having efficacy for treating prostate cancer. The comparisons may also indicate degrees of efficacy for treating prostate cancer based on the level(s) of the one or more biomarkers.

When comparing the level(s) of the one or more biomarkers in the biological sample (from a subject having high grade prostate cancer and currently or previously being treated with a composition) low grade prostate cancer-positive reference levels that distinguish over high grade prostate cancer and/or high grade prostate cancer-positive reference levels that distinguish over low grade prostate cancer, level(s) in the sample matching the low grade prostate cancer-positive reference levels that distinguish over high grade prostate cancer (e.g., levels that are the same as the reference levels, substantially the same as the reference levels, above and/or below the minimum and/or maximum of the reference levels, and/or within the range of the reference levels) are indicative of the composition having efficacy for treating prostate cancer. Levels of the one or more biomarkers in the sample matching the high grade prostate cancer-positive reference levels that distinguish over low grade prostate cancer (e.g., levels that are the same as the reference levels, substantially the same as the reference levels, above and/or below the minimum and/or maximum of the reference levels, and/or within the range of the reference levels) are indicative of the composition not having efficacy for treating prostate cancer.

When the level(s) of the one or more biomarkers in the biological sample (from a subject having prostate cancer and currently or previously being treated with a composition) are compared to level(s) of the one or more biomarkers in a previously-taken biological sample from the subject before treatment with the composition, any changes in the level(s) of the one or more biomarkers are indicative of the efficacy of the composition for treating prostate cancer. That is, if the comparisons indicate that the level(s) of the one or more biomarkers have increased or decreased after treatment with the composition to become more similar to the prostate cancer-negative reference levels (or less similar to the prostate cancer-positive reference levels) or, when the subject initially has high grade prostate cancer, the level(s) have increased or decreased to become more similar to low grade prostate cancer-positive reference levels that distinguish over high grade prostate cancer (or less similar to the high grade prostate cancer-positive reference levels that distinguish over low grade prostate cancer), then the results are indicative of the composition having efficacy for treating prostate cancer. If the comparisons indicate that the level(s) of the one or more biomarkers have not increased or decreased after treatment with the composition to become more similar to the prostate cancer-negative reference levels (or less similar to the prostate cancer-positive reference levels) or, when the subject initially has high grade prostate cancer, the level(s) have not increased or decreased to become more similar to low grade prostate cancer-positive reference levels that distinguish over high grade prostate cancer (or less similar to the high grade prostate cancer-positive reference levels that distinguish over low grade prostate cancer), then the results are indicative of the composition not having efficacy for treating prostate cancer. The comparisons may also indicate degrees of efficacy for treating prostate cancer based on the amount of changes observed in the level(s) of the one or more biomarkers after treatment. In order to help characterize such a comparison, the changes in the level(s) of the one or more biomarkers, the level(s) of the one or more biomarkers before treatment, and/or the level(s) of the one or more biomarkers in the subject currently or previously being treated with the composition may be compared to prostate cancer-positive reference levels (including low grade and high grade prostate cancer-positive reference levels), prostate cancer-negative reference levels (including low grade and high grade prostate cancer-negative reference levels), low grade prostate cancer-positive reference levels that distinguish over high grade prostate cancer, and/or high grade prostate cancer-positive reference levels that distinguish over low grade prostate cancer.

Another method for assessing the efficacy of a composition in treating prostate cancer comprises (1) analyzing a first biological sample from a subject to determine the level(s) of one or more biomarkers selected from Tables 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26, the first sample obtained from the subject at a first time point, (2) administering the composition to the subject, (3) analyzing a second biological sample from a subject to determine the level(s) of the one or more biomarkers, the second sample obtained from the subject at a second time point after administration of the composition, and (4) comparing the level(s) of one or more biomarkers in the first sample to the level(s) of the one or more biomarkers in the second sample in order to assess the efficacy of the composition for treating prostate cancer. As indicated above, if the comparison of the samples indicates that the level(s) of the one or more biomarkers have increased or decreased after administration of the composition to become more similar to the prostate cancer-negative reference levels (or less similar to the prostate cancer-positive reference levels) or, when the subject initially has high grade prostate cancer, if the level(s) have increased or decreased to become more similar to low grade prostate cancer-positive reference levels that distinguish over high grade prostate cancer (or less similar to the high grade prostate cancer-positive reference levels that distinguish over low grade prostate cancer), then the results are indicative of the composition having efficacy for treating prostate cancer. If the comparisons indicate that the level(s) of the one or more biomarkers have not increased or decreased after treatment with the composition to become more similar to the prostate cancer-negative reference levels (or less similar to the prostate cancer-positive reference levels) or, when the subject initially has high grade prostate cancer, the level(s) have not increased or decreased to become more similar to low grade prostate cancer-positive reference levels that distinguish over high grade prostate cancer (or less similar to the high grade prostate cancer-positive reference levels that distinguish over low grade prostate cancer), then the results are indicative of the composition not having efficacy for treating prostate cancer. The comparison may also indicate a degree of efficacy for treating prostate cancer based on the amount of changes observed in the level(s) of the one or more biomarkers after administration of the composition as discussed above.

A method of assessing the relative efficacy of two or more compositions for treating prostate cancer comprises (1) analyzing, from a first subject having prostate cancer and currently or previously being treated with a first composition, a first biological sample to determine the level(s) of one or more biomarkers selected from Tables 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26 (2) analyzing, from a second subject having prostate cancer and currently or previously being treated with a second composition, a second biological sample to determine the level(s) of the one or more biomarkers, and (3) comparing the level(s) of one or more biomarkers in the first sample to the level(s) of the one or more biomarkers in the second sample in order to assess the relative efficacy of the first and second compositions for treating prostate cancer. The results are indicative of the relative efficacy of the two compositions, and the results (or the levels of the one or more biomarkers in the first sample and/or the level(s) of the one or more biomarkers in the second sample) may be compared to prostate cancer-positive reference levels (including low grade and high grade prostate cancer-positive reference levels), prostate cancer-negative reference levels (including low grade and high grade prostate cancer-negative reference levels), low grade prostate cancer-positive reference levels that distinguish over high grade prostate cancer, and/or high grade prostate cancer-positive reference levels that distinguish over low grade prostate cancer to aid in characterizing the relative efficacy.

Each of the methods of assessing efficacy may be conducted on one or more subjects or one or more groups of subjects (e.g., a first group being treated with a first composition and a second group being treated with a second composition).

As with the other methods described herein, the comparisons made in the methods of assessing efficacy (or relative efficacy) of compositions for treating prostate cancer may be carried out using various techniques, including simple comparisons, one or more statistical analyses, and combinations thereof. Any suitable method may be used to analyze the biological samples in order to determine the level(s) of the one or more biomarkers in the samples. In addition, the level(s) of one or more biomarkers, including a combination of all of the biomarkers in Tables 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26 or any fraction thereof, may be determined and used in methods of assessing efficacy (or relative efficacy) of compositions for treating prostate cancer.

Finally, the methods of assessing efficacy (or relative efficacy) of one or more compositions for treating prostate cancer may further comprise analyzing the biological sample to determine the level(s) of one or more non-biomarker compounds. The non-biomarker compounds may then be compared to reference levels of non-biomarker compounds for subjects having (or not having) prostate cancer.

VI. Methods of Screening a Composition for Activity in Modulating Biomarkers Associated with Prostate Cancer

The identification of biomarkers for prostate cancer also allows for the screening of compositions for activity in modulating biomarkers associated with prostate cancer, which may be useful in treating prostate cancer. Methods of screening compositions useful for treatment of prostate cancer comprise assaying test compositions for activity in modulating the levels of one or more biomarkers in Tables 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26. Such screening assays may be conducted in vitro and/or in vivo, and may be in any form known in the art useful for assaying modulation of such biomarkers in the presence of a test composition such as, for example, cell culture assays, organ culture assays, and in vivo assays (e.g., assays involving animal models).

In one embodiment, a method for screening a composition for activity in modulating one or more biomarkers of prostate cancer comprises (1) contacting one or more cells with a composition, (2) analyzing at least a portion of the one or more cells or a biological sample associated with the cells to determine the level(s) of one or more biomarkers of prostate cancer selected from Tables 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26; and (3) comparing the level(s) of the one or more biomarkers with predetermined standard levels for the one or more biomarkers to determine whether the composition modulated the level(s) of the one or more biomarkers. As discussed above, the cells may be contacted with the composition in vitro and/or in vivo. The predetermined standard levels for the one or more biomarkers may be the levels of the one or more biomarkers in the one or more cells in the absence of the composition. The predetermined standard levels for the one or more biomarkers may also be the level(s) of the one or more biomarkers in control cells not contacted with the composition.

In addition, the methods may further comprise analyzing at least a portion of the one or more cells or a biological sample associated with the cells to determine the level(s) of one or more non-biomarker compounds of prostate cancer. The levels of the non-biomarker compounds may then be compared to predetermined standard levels of the one or more non-biomarker compounds.

Any suitable method may be used to analyze at least a portion of the one or more cells or a biological sample associated with the cells in order to determine the level(s) of the one or more biomarkers (or levels of non-biomarker compounds). Suitable methods include chromatography (e.g., HPLC, gas chromatograph, liquid chromatography), mass spectrometry (e.g., MS, MS-MS), ELISA, antibody linkage, other immunochemical techniques, and combinations thereof. Further, the level(s) of the one or more biomarkers (or levels of non-biomarker compounds) may be measured indirectly, for example, by using an assay that measures the level of a compound (or compounds) that correlates with the level of the biomarker(s) (or non-biomarker compounds) that are desired to be measured.

VII. Method of Identifying Potential Drug Targets

The identification of biomarkers for prostate cancer also allows for the identification of potential drug targets for prostate cancer. A method for identifying a potential drug target for prostate cancer comprises (1) identifying one or more biochemical pathways associated with one or more biomarkers for prostate cancer selected from Tables 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26 and (2) identifying a protein (e.g., an enzyme) affecting at least one of the one or more identified biochemical pathways, the protein being a potential drug target for prostate cancer.

Another method for identifying a potential drug target for prostate cancer comprises (1) identifying one or more biochemical pathways associated with one or more biomarkers for prostate cancer selected from Tables 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26 and one or more non-biomarker compounds of prostate cancer and (2) identifying a protein affecting at least one of the one or more identified biochemical pathways, the protein being a potential drug target for prostate cancer.

One or more biochemical pathways (e.g., biosynthetic and/or metabolic (catabolic) pathway) are identified that are associated with one or more biomarkers (or non-biomarker compounds). After the biochemical pathways are identified, one or more proteins affecting at least one of the pathways are identified. Preferably, those proteins affecting more than one of the pathways are identified.

A build-up of one metabolite (e.g., a pathway intermediate) may indicate the presence of a ‘block’ downstream of the metabolite and the block may result in a low/absent level of a downstream metabolite (e.g. product of a biosynthetic pathway). In a similar manner, the absence of a metabolite could indicate the presence of a ‘block’ in the pathway upstream of the metabolite resulting from inactive or non-functional enzyme(s) or from unavailability of biochemical intermediates that are required substrates to produce the product. Alternatively, an increase in the level of a metabolite could indicate a genetic mutation that produces an aberrant protein which results in the over-production and/or accumulation of a metabolite which then leads to an alteration of other related biochemical pathways and result in dysregulation of the normal flux through the pathway; further, the build-up of the biochemical intermediate metabolite may be toxic or may compromise the production of a necessary intermediate for a related pathway. It is possible that the relationship between pathways is currently unknown and this data could reveal such a relationship.

For example, the data indicates that metabolites in the biochemical pathways involving nitrogen excretion, amino acid metabolism, energy metabolism, oxidative stress, purine metabolism and bile acid metabolism are enriched in prostate cancer subjects. Further, polyamine levels are higher in cancer subjects, which indicates that the level and/or activity of the enzyme ornithine decarboxylase is increased. It is known that polyamines can act as mitotic agents and have been associated with free radical damage. These observations indicate that the pathways leading to the production of polyamines (or to any of the aberrant biomarkers) would provide a number of potential targets useful for drug discovery.

The proteins identified as potential drug targets may then be used to identify compositions that may be potential candidates for treating prostate cancer, including compositions for gene therapy.

VIII. Methods of Treating Prostate Cancer

The identification of biomarkers for prostate cancer also allows for the treatment of prostate cancer. For example, in order to treat a subject having prostate cancer, an effective amount of one or more prostate cancer biomarkers that are lowered in prostate cancer as compared to a healthy subject not having prostate cancer may be administered to the subject. The biomarkers that may be administered may comprise one or more of the biomarkers in Tables 1, 2, 4, 5, 6, 7, 9, 10, 13, 15, 18, 20, 22, 24, and/or 26 that are decreased in prostate cancer. In some embodiments, the biomarkers that are administered are one or more biomarkers listed in Tables 1, 2, 4, 5, 6, 7, 9, 10, 13, 15, 18, 20, 22, 24, and/or 26 that are decreased in prostate cancer and that have a p-value less than 0.10. In other embodiments, the biomarkers that are administered are one or biomarkers listed in Tables 1, 2 and/or 3 that are decreased in prostate cancer by at least 5%, by at least 10%, by at least 15%, by at least 20%, by at least 25%, by at least 30%, by at least 35%, by at least 40%, by at least 45%, by at least 50%, by at least 55%, by at least 60%, by at least 65%, by at least 70%, by at least 75%, by at least 80%, by at least 85%, by at least 90%, by at least 95%, or by 100% (i.e., absent).

IX. Methods of Using the Prostate Cancer Biomarkers for Other Types of Prostate Cancer

It is believed that some of the biomarkers for major prostate cancer described herein may also be biomarkers for other types of cancer, including, for example, lung cancer or kidney cancer. Therefore, it is believed that at least some of the prostate cancer biomarkers may be used in the methods described herein for other types of cancer. That is, the methods described herein with respect to prostate cancer may also be used for diagnosing (or aiding in the diagnosis of) any type of cancer, methods of monitoring progression/regression of any type of cancer, methods of assessing efficacy of compositions for treating any type of cancer, methods of screening a composition for activity in modulating biomarkers associated with any type of cancer, methods of identifying potential drug targets for any type of cancer, and methods of treating any type of cancer. Such methods could be conducted as described herein with respect to prostate cancer.

X. Methods of Using the Prostate Cancer Biomarkers for Other Prostate Disorders

It is believed that some of the biomarkers for prostate cancer described herein may also be biomarkers for prostate disorders (e.g. prostatitis, benign prostate hypertrophy (BHP)) in general. Therefore, it is believed that at least some of the prostate cancer biomarkers may be used in the methods described herein for prostate disorders in general. That is, the methods described herein with respect to prostate cancer may also be used for diagnosing (or aiding in the diagnosis of) a prostate disorder, methods of monitoring progression/regression of a prostate disorder, methods of assessing efficacy of compositions for treating a prostate disorder, methods of screening a composition for activity in modulating biomarkers associated with a prostate disorder, methods of identifying potential drug targets for prostate disorder, and methods of treating a prostate disorder. Such methods could be conducted as described herein with respect to prostate cancer.

XI. Other Methods

Other methods of using the biomarkers discussed herein are also contemplated. For example, the methods described in U.S. Pat. No. 7,005,255 and U.S. patent application Ser. No. 10/695,265 may be conducted using a small molecule profile comprising one or more of the biomarkers disclosed herein.

In any of the methods listed herein, the biomarkers that are used may be selected from those biomarkers in Tables 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26 having p-values of less than 0.05 and/or those biomarkers in Tables 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26 having q-values of less than 0.10. The biomarkers that are used in any of the methods described herein may also be selected from those biomarkers in Tables 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26 that are decreased in prostate cancer (as compared to the control) or that are decreased in remission (as compared to control or prostate cancer) by at least 5%, by at least 10%, by at least 15%, by at least 20%, by at least 25%, by at least 30%, by at least 35%, by at least 40%, by at least 45%, by at least 50%, by at least 55%, by at least 60%, by at least 65%, by at least 70%, by at least 75%, by at least 80%, by at least 85%, by at least 90%, by at least 95%, or by 100% (i.e., absent); and/or those biomarkers in Tables 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26 that are increased in prostate cancer (as compared to the control or remission) or that are increased in remission (as compared to the control or prostate cancer) by at least 5%, by at least 10%, by at least 15%, by at least 20%, by at least 25%, by at least 30%, by at least 35%, by at least 40%, by at least 45%, by at least 50%, by at least 55%, by at least 60%, by at least 65%, by at least 70%, by at least 75%, by at least 80%, by at least 85%, by at least 90%, by at least 95%, by at least 100%, by at least 110%, by at least 120%, by at least 130%, by at least 140%, by at least 150%, or more.

EXAMPLES

The invention will be further explained by the following illustrative examples that are intended to be non-limiting.

I. General Methods

A. Identification of Metabolic Profiles for Prostate Cancer

Each sample was analyzed to determine the concentration of several hundred metabolites. Analytical techniques such as GC-MS (gas chromatography-mass spectrometry) and LC-MS (liquid chromatography-mass spectrometry) were used to analyze the metabolites. Multiple aliquots were simultaneously, and in parallel, analyzed, and, after appropriate quality control (QC), the information derived from each analysis was recombined. Every sample was characterized according to several thousand characteristics, which ultimately amount to several hundred chemical species. The techniques used were able to identify novel and chemically unnamed compounds.

B. Statistical Analysis

The data was analyzed using T-tests to identify molecules (either known, named metabolites or unnamed metabolites) present at differential levels in a definable population or subpopulation (e.g., biomarkers for prostate cancer biological samples compared to control biological samples or compared to patients in remission from prostate cancer) useful for distinguishing between the definable populations (e.g., prostate cancer and control, low grade prostate cancer and high grade prostate cancer). Other molecules (either known, named metabolites or unnamed metabolites) in the definable population or subpopulation were also identified.

Data was also analyzed using Random Forest Analysis. Random forests give an estimate of how well individuals in a new data set can be classified into existing groups. Random forest analysis creates a set of classification trees based on continual sampling of the experimental units and compounds. Then each observation is classified based on the majority votes from all the classification trees. In statistics, a classification tree classifies the observations into groups based on combinations of the variables (in this instance variables are metabolites or compounds). There are many variations on the algorithms used to create trees. A tree algorithm searches for the metabolite (compound) that provides the largest split between the two groups. This produces nodes. Then at each node, the metabolite that provides the best split is used and so on. If the node cannot be improved on, then it stops at that node and any observation in that node is classified as the majority group.

Random forests classify based on a large number (e.g. thousands) of trees. A subset of compounds and a subset of observations are used to create each tree. The observations used to create the tree are called the in-bag samples, and the remaining samples are called the out-of-bag samples. The classification tree is created from the in-bag samples, and the out-of-bag samples are predicted from this tree. To get the final classification for an observation, the “votes” for each group are counted based on the times it was an out-of-bag sample. For example, suppose observation 1 was classified as a “Control” by 2,000 trees, but classified as “Disease” by 3,000 trees. Using “majority wins” as the criterion, this sample is classified as “Disease.”

The results of the random forest are summarized in a confusion matrix. The rows correspond to the true grouping, and the columns correspond to the classification from the random forest. Thus, the diagonal elements indicate the correct classifications. A 50% error would occur by random chance for 2 groups, 66.67% error for three groups by random chance, etc. The “Out-of-Bag” (OOB) Error rate gives an estimate of how accurately new observations can be predicted using the random forest model (e.g., whether a sample is from a diseased subject or a control subject).

It is also of interest to see which variables are more “important” in the final classifications. The “importance plot” shows the top compounds ranked in terms of their importance. There are different criteria for ranking the importance, but the general idea is that removing an important variable will cause a greater decrease in accuracy than a variable that is less important.

C. Biomarker Identification

Various peaks identified in the analyses (e.g. GC-MS, LC-MS, MS-MS), including those identified as statistically significant, were subjected to a mass spectrometry based chemical identification process.

Example 1 Tissue

Biomarkers were discovered by (1) analyzing tissue samples from different groups of human subjects to determine the levels of metabolites in the samples and then (2) statistically analyzing the results to determine those metabolites that were differentially present in the two groups.

The tissue samples used for the analysis were 16 control tissues that were cancer free tissues derived from sections of prostate tissue not containing cancer cells (i.e. from cancerous prostate glands and that were determined to be free of cancerous cells), 12 prostate tissue samples from localized prostate cancer tumors (i.e. lower grade prostate cancer) and 14 prostate tissue samples from distal metastatic prostate cancer tumors (i.e. high grade prostate cancer). After the levels of metabolites were determined, the data was analyzed using univariate T-tests (i.e., Welch's T-test).

T-tests were used to determine differences in the mean levels of metabolites between two populations (i.e., Lower Grade Prostate Cancer vs. Control, Metastatic/High Grade Prostate Cancer vs. Control, Metastatic/High Grade Prostate Cancer vs. Lower Grade Prostate Cancer).

Biomarkers:

As listed below in Table 1, biomarkers were discovered that were differentially present between tissue samples from lower grade, localized prostate cancer tumors and Control prostate tissue that was determined to be free of cancerous cells (i.e. sections of prostate tissue not containing cancerous cells from cancerous prostate glands removed from the patient). Table 2 lists biomarkers that were discovered that were differentially present between tissue from prostate tumor samples from subjects with metastatic prostate cancer (i.e. high grade prostate cancer) and Control prostate tissue. Table 3 lists biomarkers that were discovered that were differentially present between tissue samples from prostate tumor samples from subjects with metastatic prostate cancer (i.e. high grade prostate cancer) and tissue samples from lower grade, localized prostate cancer tumors.

Tables 1-3 include, for each listed biomarker, the p-value and the q-value determined in the statistical analysis of the data concerning the biomarkers and an indication of the percentage difference in the lower grade prostate cancer (PCA) mean level as compared to the control mean level (Table 1), the high grade prostate cancer mean level as compared to the control mean level (Table 2), and the high grade prostate cancer mean level as compared to the lower grade prostate cancer mean level (Table 3). The term “Isobar” as used in the tables indicates the compounds that could not be distinguished from each other on the analytical platform used in the analysis (i.e., the compounds in an isobar elute at nearly the same time and have similar (and sometimes exactly the same) quant ions, and thus cannot be distinguished). Library indicates the chemical library that was used to identify the compounds. The number 50 refer to the GC library and the number 61 refers to the LC library.

TABLE 1 Prostate Cancer Biomarkers from subjects with Lower Grade Prostate Cancer compared to Control subjects. % Li- Change COMPOUND brary p-value q-value in PCA Metabolite-3139 61 <0.0001 0.0019 147% Metabolite-1114 61 <0.0001 0.0053 55% uridine 61 1.00E-04 0.0064 71% Metabolite-3778 61 1.00E-04 0.0064 −67% dethiobiotin 50 1.00E-04 0.0064 62% Metabolite-3094 50 1.00E-04 0.0075 62% N-acetyl-D-galactosamine 50 2.00E-04 0.0092 214% 4-hydroxy-2- 61 3.00E-04 0.0092 110% quinolinecarboxylic acid Metabolite-4019 50 3.00E-04 0.0092 104% Metabolite-2688 61 3.00E-04 0.0092 20% proline 50 3.00E-04 0.0092 59% Metabolite-1111-possible- 61 3.00E-04 0.0092 92% methylnitronitroso- guanidine-or-ethyl- thiocarbamoylacetate glutamic acid 50 4.00E-04 0.0095 83% 3-hydroxy-3- 50 5.00E-04 0.0107 82% methylglutarate Metabolite-3810 61 6.00E-04 0.0119 −45% Metabolite-1576 61 6.00E-04 0.0119 116% Metabolite-4637 50 7.00E-04 0.0134 55% Metabolite-1595-possible- 61 7.00E-04 0.0134 −58% glutathione-metabolite glycine 50 8.00E-04 0.0142 67% leucine 50 9.00E-04 0.0145 61% threonine 50 9.00E-04 0.0145 51% histidine 50 0.001 0.0151 58% anthranilic acid 50 0.0012 0.0167 53% asparagine 50 0.0012 0.0167 81% L-allo-threonine 50 0.0014 0.0177 48% n-hexadecanoic acid 50 0.0014 0.0177 36% 1-7-dihydro-6h-purin- 61 0.0016 0.0193 43% 6-one N-acetyl-D-glucosamine 50 0.0016 0.0193 125% DL-homocysteine 61 0.0019 0.021 118% sn-Glycerol-3-phosphate 50 0.0019 0.021 98% Isobar-2-includes-3- 61 0.0021 0.0219 58% amino-isobutyrate-2- amino-butyrate-4- aminobutanoic acid- dimethylglycine-choline- 3-phospho-l-serine 61 0.0023 0.0228 18% Isobar-27-includes-L- 61 0.0024 0.0228 90% kynurenine-alpha-2- diamino-gamma- oxobenzenebutanoic acid Metabolite-4051 50 0.0024 0.0228 108% alpha-amino-adipate 50 0.0026 0.0228 99% Metabolite-4117-possible- 61 0.0026 0.0228 163% propranolol-or-2-heptyl-3- hydroxy-quinolone cholesterol 50 0.0027 0.0228 46% Metabolite-5128 61 0.0027 0.0228 −85% Isobar-6-includes-valine- 61 0.0029 0.0228 36% betaine Metabolite-4616 61 0.0029 0.0228 269% Metabolite-4015 50 0.0029 0.0228 102% Metabolite-2973 50 0.0029 0.0228 −25% valine 50 0.003 0.0233 38% malic acid 50 0.0032 0.0237 62% Metabolite-1211 61 0.0033 0.0241 −52% Isobar-22-includes- 61 0.0037 0.0263 44% glutamic acid-O-acetyl-L-serine tetradecanoic acid 50 0.0038 0.0263 59% phosphate 50 0.0039 0.0265 68% possible-ISOBAR-DL- 50 0.004 0.0267 71% aspartic acid- Metabolite-2466 61 0.0041 0.0271 99% Metabolite-2548 61 0.0044 0.0283 −32% Metabolite-3848 61 0.0045 0.0283 117% Metabolite-2109 61 0.0046 0.0283 120% tryptophan 61 0.0046 0.0283 38% 2-acetamido-1-amino-1-2- 50 0.0054 0.0324 134% dideoxy-beta-D- glucopyranose Metabolite-3998 50 0.0057 0.0324 53% 5-oxoproline 50 0.0057 0.0324 48% riboflavine 61 0.0058 0.0324 67% phytonadione 50 0.0059 0.0324 45% Metabolite-2074 61 0.0059 0.0324 −42% 9-12-octadecadienoic 50 0.0061 0.0328 74% acid-z-z-carnitine 61 0.0063 0.033 47% Metabolite-3370 61 0.0063 0.033 37% uracil 50 0.0067 0.0343 129% noradrenaline 50 0.0068 0.0344 50% tyrosine 61 0.007 0.0348 41% cysteine 50 0.0073 0.036 800% 25-hydroxycholesterol 50 0.0075 0.0364 18% Metabolite-4030-possible- 61 0.0076 0.0366 109% glutethimide-or-securinine N-acetylserotonin 50 0.008 0.0376 279% Metabolite-2108 61 0.0081 0.0376 78% phenylalanine 61 0.0082 0.0376 36% Isobar-3-includes-inositol- 61 0.0088 0.0395 63% 1- phosphate-mannose-6- phosphate-glucose-6- phosphate-D-mannose-1- phosphate-alpha-D- glucose-1-phosphate-alpha- D-galactose-1-phosphate Metabolite-1713 61 0.0089 0.0395 82% Metabolite-1977 61 0.0094 0.0412 218% octadecanoic acid 50 0.0099 0.0429 25% 3-nitro-L-tyrosine 50 0.0101 0.0434 82% Metabolite-2064 61 0.0112 0.0472 44% Metabolite-2389 61 0.0123 0.051 36% Metabolite-4617 61 0.0124 0.051 53% orotidine-5-phosphate 61 0.013 0.0528 125% serine 50 0.0135 0.0542 40% spermine 50 0.0143 0.0565 −78% Metabolite-2041 61 0.0145 0.0565 157% Metabolite-1465 61 0.0146 0.0565 174% N-5-aminocarbonyl- 50 0.0158 0.0607 136% L-ornithine 2-deoxy-D-ribose 61 0.0164 0.062 44% heptadecanoic acid 50 0.0168 0.0622 76% Metabolite-3165 61 0.0172 0.0622 26% methionine 61 0.0173 0.0622 43% S-adenosyl-l-homocysteine 61 0.0173 0.0622 41% Isobar-24-includes-L- 61 0.0174 0.0622 46% arabitol-adonitol glycerol 50 0.0175 0.0622 51% Metabolite-2690 61 0.019 0.0662 147% Metabolite-3176-possible- 61 0.0191 0.0662 −22% creatine Metabolite-4632 50 0.0197 0.0675 44% aspartate 61 0.0207 0.0695 54% Metabolite-3027 50 0.0207 0.0695 108% mannose-6-phosphate 50 0.022 0.0734 179% Metabolite-5215 50 0.0225 0.0742 −27% Metabolite-2055 61 0.0229 0.0744 −35% uridine-5-monophosphate 61 0.023 0.0744 −38% Metabolite-4046 50 0.0249 0.0797 305% Metabolite-4355 50 0.0256 0.0797 36% Metabolite-4058 50 0.0256 0.0797 104% Carnosine 61 0.0256 0.0797 −45% Metabolite-1070 61 0.0263 0.0811 109% Metabolite-5228 50 0.0279 0.0852 52% Metabolite-2753 61 0.0286 0.0861 224% Metabolite-4116 61 0.0289 0.0861 34% Metabolite-2272 61 0.0292 0.0861 152% Metabolite-4027 50 0.0294 0.0861 145% xanthine 61 0.0298 0.0861 172% Metabolite-2924 50 0.0298 0.0861 51% N-N-dimethylarginine 61 0.0318 0.0911 224% Metabolite-4017 50 0.0322 0.0915 52% glutamine 50 0.0333 0.0924 39% isoleucine 50 0.0335 0.0924 26% Metabolite-1498 61 0.0336 0.0924 48% adenine 50 0.0336 0.0924 65% Metabolite-2005 61 0.0345 0.0941 45% sarcosine 50 0.0354 0.0958 150% Metabolite-3498 61 0.0366 0.098 55% Metabolite-5210 50 0.0396 0.1052 −23% arginino-succinate 61 0.043 0.1132 93% Putrescine 50 0.0432 0.1132 −82% Metabolite-1104 61 0.0441 0.1144 −35% taurine 61 0.0455 0.1171 −21% Metabolite-1597 61 0.0461 0.1178 29% Metabolite-4043 50 0.0469 0.119 33% Metabolite-3183-possible- 61 0.0475 0.1195 107% gamma-L-glutamyl-L- phenylalanine-or- aspartame N-6-trimethyl-l-lysine 61 0.0486 0.1215 35% Metabolite-2250 61 0.0508 0.1261 71% creatinine 61 0.0514 0.1261 −25% melatonin 50 0.0516 0.1261 111% Metabolite-2105 61 0.0541 0.1311 100% 2-deoxyuridine-5- 61 0.0571 0.1375 −41% triphosphate tyramine 50 0.0591 0.1404 32% inositol-1-phosphate 50 0.0592 0.1404 40% 4-methyl-2-oxopentanoate 61 0.0597 0.1405 162% Metabolite-5186 61 0.0601 0.1406 600% fumaric acid 50 0.0638 0.1482 82% 2-deoxyuridine 61 0.0676 0.156 74% Metabolite-1085-possible- 61 0.0688 0.1576 45% isolobinine-or-4- aminoestra-1-3-5-10- triene-3-17beta-diol Metabolite-4868-possible- 61 0.0703 0.1598 114% Bradykinin Metabolite-2846 61 0.0753 0.1701 141% arachidonic acid 50 0.0765 0.1705 52% Metabolite-1831-possible- 61 0.0769 0.1705 30% Cl-adduct-of-citrulline Metabolite-3099 50 0.0771 0.1705 43% trans-4-hydroxyproline 50 0.0776 0.1705 63% Metabolite-3783 61 0.0782 0.1708 −39% L-alpha- 61 0.0793 0.172 58% glycerophosphorylcholine glycerate 61 0.0798 0.172 42% cytidine 61 0.0819 0.1741 101% Isobar-40-includes- 61 0.0825 0.1741 −41% Maltotetraose-stachyose Metabolite-1679 61 0.0831 0.1741 347% Metabolite-4032 50 0.0836 0.1741 108% Metabolite-3752 61 0.0841 0.1741 756% Isobar-32-includes-N- 61 0.0847 0.1741 34% acetyl-D-glucosamine-N- acetyl-D-mannosamine pantothenic acid 61 0.0849 0.1741 40% glyceric acid 50 0.085 0.1741 27% xylitol 50 0.0907 0.1831 65% Metabolite-2075 61 0.0915 0.1831 148% Metabolite-3430 61 0.0916 0.1831 63% Metabolite-3668 61 0.0917 0.1831 −47% 5-6-dihydrouracil 61 0.0928 0.1831 94% Metabolite-3138 61 0.0933 0.1831 62% Metabolite-2056 61 0.0933 0.1831 −20% Metabolite-4362 50 0.0944 0.1834 −41% Metabolite-4514 50 0.095 0.1834 −19% Metabolite-2607 61 0.0959 0.1834 −45% Isobar-21-includes-gamma- 61 0.096 0.1834 70% aminobutyryl-L-histidine-L- anserine Isobar-5-includes- 61 0.0963 0.1834 57% asparagine-ornithine Metabolite-3957 61 0.0968 0.1834 43% Isobar-30-includes- 61 0.0993 0.1867 −35% maltotetraose-stachyose D-sorbitol-6-phosphate 50 0.0996 0.1867 53% Metabolite-2981 50 0.1017 0.1894 17% ribulose-5-phosphate 50 0.1041 0.1929 −25% Metabolite-3123 61 0.1082 0.1987 −42% Isobar-18-includes-D- 61 0.1085 0.1987 67% fructose-1-phosphate-beta- D-fructose-6-phosphate Metabolite-1593 61 0.111 0.2022 −44% uric acid 61 0.1119 0.2027 −19% Metabolite-3178 61 0.1128 0.2027 −30% Metabolite-1455 61 0.1131 0.2027 −81% Metabolite-1286 61 0.1145 0.204 −16% Isobar-1-includes-mannose- 61 0.1166 0.2068 −35% fructose-glucose-galactose- alpha-L-sorbopyranose- Inositol-D-allose o-phosphoethanolamine 50 0.1196 0.211 44% Metabolite-1608 61 0.1206 0.2115 −59% Metabolite-3539 61 0.1217 0.2123 −57% Metabolite-4593 50 0.1257 0.217 37% palmitoleic acid 50 0.1257 0.217 78% Metabolite-3896 61 0.1274 0.2176 65% 1-methyladenosine 61 0.1274 0.2176 100% Metabolite-1203-possible- 61 0.1333 0.2253 86% acetylbrowniine-tricornine- germine-or-veracevine Metabolite-3771 61 0.1338 0.2253 −18% pyridoxamine-phosphate 61 0.1341 0.2253 −33% Metabolite-2212 61 0.135 0.2253 320% Spermidine 50 0.1367 0.2253 −51% Metabolite-3992- 61 0.1375 0.2253 14% Metabolite-3044 61 0.1385 0.2253 30% 3-methyl-L-histidine 61 0.1389 0.2253 21% Metabolite-2546 61 0.1393 0.2253 −36% fructose 50 0.1396 0.2253 −47% Metabolite-3816 61 0.1397 0.2253 −43% Metabolite-2255 61 0.1406 0.2253 44% Metabolite-3073 50 0.1407 0.2253 −39% succinate 50 0.1456 0.2314 −54% Metabolite-2292 61 0.1459 0.2314 −35% glutathione-reduced 61 0.1467 0.2314 −43% alanine 50 0.1494 0.2346 21% Metabolite-4053 50 0.1527 0.2387 26% Metabolite-4567 61 0.1555 0.2419 −37% Metabolite-3832-possible- 61 0.1614 0.2499 −30% phenol-sulfate Metabolite-5189 61 0.1668 0.2571 263% saccharopine 61 0.1679 0.2575 23% Metabolite-1216 61 0.1699 0.2577 53% Metabolite-5227 50 0.1704 0.2577 45% citric acid 50 0.1708 0.2577 −37% catechol 61 0.1712 0.2577 77% Metabolite-4615 61 0.1733 0.2594 −13% Metabolite-3808 61 0.1747 0.2594 −20% Metabolite-1609 61 0.1753 0.2594 −35% D-allose 50 0.1754 0.2594 −37% elaidic acid 50 0.1821 0.2681 84% Metabolite-2129 61 0.1835 0.269 110% Metabolite-2185 61 0.1864 0.2706 35% azelaic acid 61 0.1875 0.2706 63% Metabolite-1088 61 0.1879 0.2706 81% Metabolite-5232 50 0.1879 0.2706 109% Isobar-17-includes- 61 0.1887 0.2706 25% arginine-N- alpha-acetyl-ornithine hypotaurine 50 0.1917 0.2736 36% Metabolite-4150 50 0.1936 0.2744 −36% Metabolite-2111 61 0.1939 0.2744 33% Metabolite-1457 61 0.1957 0.2758 −35% DL-cystathionine 50 0.1983 0.2783 22% Metabolite-5147 61 0.2019 0.2822 243% Metabolite-3476 61 0.2033 0.2828 −23% benzoic acid 50 0.2043 0.2831 −14% Metabolite-5109 61 0.2069 0.2843 89% Metabolite-3102 50 0.2082 0.2843 25% Metabolite-3974 61 0.2083 0.2843 38% Metabolite-1351 61 0.2086 0.2843 19% mannose 50 0.2108 0.2858 −32% quinolinic acid 61 0.2114 0.2858 42% gamma-L-glutamyl- 61 0.2161 0.2896 −28% L-glutamine Metabolite-1186 61 0.2164 0.2896 −54% Metabolite-2766 61 0.2183 0.2896 −34% phosphoenolpyruvate 50 0.2184 0.2896 105% Metabolite-4080 50 0.2187 0.2896 71% Metabolite-2139 61 0.221 0.2915 41% Metabolite-2900- 61 0.222 0.2915 24% Metabolite-2388 61 0.2232 0.2915 24% 2-deoxy-D-glucose 50 0.2236 0.2915 −30% 5-hydroxyindoleacetate 50 0.2255 0.2928 210% Metabolite-4869 61 0.2292 0.2964 −38% Metabolite-2774 61 0.2377 0.306 47% Metabolite-2232 61 0.2385 0.306 −36% 3-methoxy-L-tyrosine 50 0.2401 0.3069 −29% inositol 50 0.2414 0.3071 −29% glucono-gamma-lactone 50 0.2434 0.3071 −31% Metabolite-4133 50 0.2441 0.3071 34% Metabolite-4014 50 0.2444 0.3071 −17% galactose 50 0.2449 0.3071 30% Metabolite-3813 61 0.248 0.3097 115% Metabolite-1980 61 0.2537 0.3151 119% Metabolite-5108 61 0.2542 0.3151 72% Metabolite-2703 61 0.2562 0.3165 31% Metabolite-5110 61 0.2632 0.3239 63% Metabolite-5207 50 0.2649 0.3247 13% Metabolite-2027 61 0.2671 0.3262 47% 2-keto-L-gulonic acid 50 0.2795 0.34 12% Metabolite-3064 61 0.2832 0.3434 50% glucose-6-phosphate 50 0.2849 0.3439 −28% Metabolite-5166 61 0.2857 0.3439 49% 3-amino-isobutyrate 50 0.2892 0.3457 −27% dulcitol 50 0.2894 0.3457 −27% Metabolite-3034 50 0.2933 0.349 22% Metabolite-4667 61 0.2942 0.349 18% Metabolite-2806 61 0.2996 0.3541 −12% Metabolite-5089 61 0.3011 0.3541 −65% 4-hydroxyphenylpyruvate 61 0.3034 0.3541 −17% Metabolite-4075 50 0.3039 0.3541 36% Metabolite-4235 61 0.3039 0.3541 −67% glutarate 61 0.3122 0.358 47% beta-nicotinamide-adenine- 61 0.3134 0.358 445% dinucleotide Metabolite-1327-possible- 61 0.3175 0.358 30% bilirubin guanine 50 0.3177 0.358 26% Metabolite-1323-possible- 61 0.3181 0.358 −31% 4-sulfobenzyl-alcohol Metabolite-3708 61 0.3196 0.358 −10% Metabolite-4706 61 0.3202 0.358 42% Metabolite-3545 61 0.3204 0.358 76% Metabolite-3132 61 0.3217 0.358 23% niacinamide 61 0.3243 0.358 12% Metabolite-3514-retired- 61 0.3248 0.358 −89% topiramate Metabolite-5167 61 0.3248 0.358 43% Metabolite-5170 61 0.3251 0.358 854% Metabolite-3951 61 0.3267 0.358 13% Metabolite-2768 61 0.3321 0.358 798% allantoin 61 0.3332 0.358 −15% Metabolite-2347 61 0.3332 0.358 −16% Metabolite-3436 61 0.3332 0.358 −21% Metabolite-5087 61 0.3338 0.358 −53% Metabolite-3576 61 0.3374 0.358 23% Metabolite-3694 61 0.3383 0.358 36% Metabolite-3522 61 0.3398 0.358 −85% Metabolite-2406 61 0.3409 0.358 31% Metabolite-3364 61 0.3409 0.358 12% Metabolite-3997 61 0.3409 0.358 43% Metabolite-4018 61 0.3409 0.358 38% suberic acid 61 0.3409 0.358 15% Metabolite-3022 50 0.3409 0.358 8% Metabolite-1329 61 0.3409 0.358 10% Metabolite-3756 61 0.3409 0.358 25% Metabolite-5086 61 0.3409 0.358 −41% Metabolite-1911 61 0.3431 0.3582 64% gamma-glu-cys 61 0.3432 0.3582 60% N-acetylneuraminate 61 0.3469 0.3609 −14% Metabolite-2691 61 0.3544 0.3669 33% Metabolite-3531 61 0.3572 0.3669 −70% Metabolite-3180 61 0.3575 0.3669 46% L-homoserine-lactone 61 0.3583 0.3669 12% Metabolite-1974 61 0.3584 0.3669 −18% Metabolite-2141 61 0.3594 0.3669 29% Metabolite-1333 61 0.3625 0.3684 28% GABA 50 0.3631 0.3684 −20% adenosine 61 0.3676 0.3719 −18% Metabolite-5226 50 0.3709 0.3741 33% Metabolite-2036 61 0.3733 0.3748 −38% Metabolite-1616 61 0.3739 0.3748 76% Metabolite-3833 61 0.3752 0.375 20% Metabolite-2348 61 0.3836 0.3813 69% S-5-adenosyl-L-methionine 61 0.3839 0.3813 30% Metabolite-4331 61 0.3854 0.3817 26% Metabolite-3475 61 0.3873 0.3824 −19% n-dodecanoate 50 0.3957 0.3895 15% Metabolite-3952 61 0.399 0.3914 −14% Metabolite-3837 61 0.3999 0.3914 −33% Metabolite-1819 61 0.4015 0.3917 −15% Metabolite-2853 61 0.4036 0.3926 −20% Metabolite-3517 61 0.4048 0.3926 −34% Metabolite-3526 61 0.4155 0.4018 −23% Metabolite-2711 61 0.4182 0.4029 11% 5-s-methyl-5- 61 0.419 0.4029 33% thioadenosine xanthosine 50 0.4265 0.4088 −18% Metabolite-5107 61 0.4345 0.4153 36% Metabolite-1248-possible- 61 0.4438 0.421 22% avermectin-aglycone ornithine 50 0.4438 0.421 19% Metabolite-3984 61 0.4443 0.421 58% Metabolite-3215 61 0.4466 0.4219 −18% Metabolite-2181 61 0.45 0.423 23% Metabolite-1392 61 0.4505 0.423 −49% Metabolite-4512 50 0.4516 0.423 34% Metabolite-5209 50 0.4539 0.4241 −16% Metabolite-2198 61 0.4573 0.4251 −19% Metabolite-4931 61 0.4578 0.4251 11% Metabolite-3604 61 0.4589 0.4251 30% maltose 50 0.4614 0.4253 −13% Metabolite-1330 61 0.4623 0.4253 −50% Metabolite-1843 61 0.4644 0.4253 35% Metabolite-5214 50 0.4665 0.4253 −19% Metabolite-3056 61 0.467 0.4253 −23% Metabolite-4084 50 0.468 0.4253 −5% Metabolite-2567 61 0.4682 0.4253 15% Metabolite-3893 61 0.4774 0.4323 −14% Metabolite-3543 61 0.4785 0.4323 −47% Metabolite-4503 50 0.4815 0.4338 21% Isobar-31-includes- 61 0.4912 0.4406 −14% maltotriose-melezitose histamine 61 0.4917 0.4406 −13% D-ribose 50 0.4931 0.4407 −17% Metabolite-3390 61 0.4987 0.4445 −4% 6-phosphogluconic acid 61 0.5166 0.4592 −7% Metabolite-2319 61 0.5186 0.4597 24% lactate 50 0.523 0.4624 8% Metabolite-4096- 61 0.5325 0.4695 −7% gamma-glu-gly-leu- Metabolite-4518 50 0.5347 0.4701 22% Metabolite-1129 61 0.536 0.4701 23% Metabolite-3003 50 0.5401 0.4724 15% Metabolite-5213 50 0.5456 0.476 −11% Metabolite-1069-possible- 61 0.549 0.4771 25% dehydroepiandrosterone- sulfate- Metabolite-1575 61 0.5511 0.4771 −16% 3-hydroxybutanoic acid 50 0.5512 0.4771 −22% Metabolite-4238 61 0.553 0.4773 14% pyrophosphate 50 0.5551 0.4779 20% Metabolite-2867 61 0.5592 0.4788 25% Metabolite-1718 61 0.5602 0.4788 20% arabinose 50 0.5604 0.4788 −14% Metabolite-3401 61 0.5676 0.4836 −18% beta-alanine 50 0.5697 0.4842 −12% Metabolite-2897 61 0.5738 0.4856 −13% Metabolite-1394-possible- 61 0.5743 0.4856 23% Losartan Metabolite-4428 61 0.5759 0.4857 21% Metabolite-2099 61 0.5866 0.4924 26% Metabolite-3220 61 0.5868 0.4924 10% Metabolite-3317 61 0.5908 0.4932 19% biliverdin 61 0.5908 0.4932 −12% Metabolite-3002 50 0.5925 0.4934 6% Metabolite-3955 61 0.5991 0.4976 −4% Metabolite-3020 50 0.6009 0.4979 12% Metabolite-3189 61 0.6061 0.5009 −23% Metabolite-1970 61 0.6121 0.5046 19% Metabolite-1963 61 0.6203 0.5078 −9% Metabolite-1113-possible- 61 0.6216 0.5078 −6% acetylcarnitine-or- isopentyl-adenine Metabolite-3016 50 0.6232 0.5078 −12% caffeine 61 0.6241 0.5078 22% ethylmalonic acid 61 0.6247 0.5078 38% cystine 50 0.6255 0.5078 8% Metabolite-2558 61 0.6268 0.5078 −21% uridine-5- 50 0.629 0.5084 8% diphosphoglucose 3-methyl-2-oxovaleric acid 61 0.634 0.5112 26% dihydroxyacetone- 61 0.6396 0.5144 11% phosphate Metabolite-4497 50 0.6462 0.5184 −12% Metabolite-2313 61 0.649 0.5184 7% Metabolite-3085 50 0.6493 0.5184 −5% Metabolite-3996 50 0.6552 0.521 −9% L-histidinol 61 0.6557 0.521 −9% Metabolite-1573 61 0.6598 0.5231 −9% Metabolite-2407 61 0.6624 0.5238 −18% Metabolite-5126 61 0.665 0.5246 11% Metabolite-4448 61 0.6685 0.5261 −8% alpha-D-ribose-5- 50 0.6795 0.5319 9% phosphate cytidine-5- 61 0.6818 0.5319 11% monophosphate Metabolite-1979-Cl- 61 0.6823 0.5319 5% adduct-of-C6H10O5 Metabolite-2-Aminoethyl- 61 0.6831 0.5319 4% phosphonate sorbitol 50 0.6839 0.5319 −21% Metabolite-2368 61 0.6862 0.5324 56% Metabolite-1961-retired- 61 0.7054 0.5461 45% glycocholic acid Metabolite-4523 50 0.7076 0.5464 7% alpha-4- 50 0.7136 0.5498 20% dihydroxybenzene- propanoic acid Metabolite-1342-possible- 61 0.728 0.5591 −15% phenylacetylglutamine Metabolite-4020 50 0.7305 0.5591 9% Metabolite-3554 61 0.7316 0.5591 14% Metabolite-2174 61 0.7325 0.5591 9% Metabolite-4002 50 0.7391 0.5629 8% DL-pipecolic acid 61 0.7474 0.5674 −8% Metabolite-2824 61 0.7484 0.5674 14% Metabolite-3807 61 0.7516 0.5685 −4% Metabolite-3129 61 0.7585 0.5724 −3% Metabolite-2194 61 0.7641 0.5735 −9% ascorbic acid 50 0.7647 0.5735 −10% biotin 61 0.7657 0.5735 −9% Metabolite-1975 61 0.7669 0.5735 −8% Metabolite-1349 61 0.7721 0.576 −6% Metabolite-2072 61 0.7799 0.5792 −9% Metabolite-1142- 61 0.7817 0.5793 4% possible-5-hydroxy- pentanoate-or-beta- hydroxyisovaleric acid Metabolite-4806 50 0.7875 0.581 3% Metabolite-4796 50 0.7882 0.581 −9% 4-Guanidinobutanoic acid 61 0.7894 0.581 6% Metabolite-3489 61 0.7984 0.5855 −7% Metabolite-1116 61 0.799 0.5855 −5% Metabolite-2827 61 0.8024 0.5867 13% Metabolite-3772 61 0.814 0.5923 4% Metabolite-2143 61 0.8147 0.5923 −12% Metabolite-3960 61 0.8168 0.5923 2% Metabolite-3040 50 0.8172 0.5923 3% Metabolite-3994 61 0.8202 0.5931 −8% Metabolite-2180 61 0.8237 0.5944 −7% Metabolite-2118 61 0.8311 0.5974 −3% Metabolite-4787 61 0.8315 0.5974 15% Metabolite-4516 50 0.8341 0.5979 −6% Metabolite-4168 61 0.8386 0.598 4% uridine-5- 50 0.841 0.598 7% diphosphoglucuronic acid Metabolite-4134 50 0.8433 0.598 3% Metabolite-4271 50 0.8442 0.598 −17% Metabolite-2121 61 0.8444 0.598 8% Metabolite-4013 61 0.8451 0.598 5% urea 50 0.8512 0.6007 −2% Metabolite-4272 50 0.8534 0.6007 −4% Metabolite-1653 61 0.855 0.6007 6% Metabolite-1183 61 0.8561 0.6007 9% Metabolite-5229 50 0.8587 0.6012 3% glucarate 50 0.8678 0.6063 14% Metabolite-1187 61 0.8864 0.6163 5% beta-D-lactose 50 0.8875 0.6163 3% Metabolite-2279 61 0.8877 0.6163 −4% Metabolite-5212 50 0.8914 0.617 3% alpha-L-sorbopyranose 50 0.8925 0.617 −3% Metabolite-4354 50 0.9014 0.6219 −2% Metabolite-3014 50 0.9104 0.6256 1% Metabolite-3534 61 0.9131 0.6256 −5% Metabolite-3966 61 0.9137 0.6256 −4% Metabolite-1497 61 0.9149 0.6256 −2% Metabolite-3379 61 0.9178 0.6256 −2% Metabolite-1288 61 0.9188 0.6256 3% Metabolite-2237 61 0.9222 0.6256 4% Metabolite-3755 61 0.9248 0.6256 2% Metabolite-3980 61 0.9253 0.6256 −3% picolinic acid 61 0.9259 0.6256 3% Metabolite-2821 61 0.9284 0.6261 −2% L-kynurenine 50 0.9317 0.627 −3% inosine 61 0.9399 0.6272 −1% Metabolite-2724 61 0.9415 0.6272 −1% Isobar-19-includes- 61 0.9431 0.6272 1% D-saccharic acid- 2-deoxy-D-galactose- 2-deoxy-D-glucose-L- fucose-L-rhamnose Metabolite-4510 50 0.9434 0.6272 −1% alpha-keto-glutarate 61 0.9448 0.6272 −6% 3-methylglutaric acid 61 0.9453 0.6272 0% Metabolite-3051 61 0.9454 0.6272 2% Metabolite-3484 61 0.9472 0.6272 −3% Metabolite-1303 61 0.9513 0.6276 −2% Metabolite-3074 50 0.9517 0.6276 3% guanosine 61 0.9553 0.6288 0% hippuric acid 61 0.9589 0.6288 2% Metabolite-5211 50 0.9591 0.6288 3% Metabolite-5187 61 0.9644 0.6297 1% Metabolite-1496 61 0.9648 0.6297 0% Metabolite-4550 61 0.9663 0.6297 −2% Metabolite-3365 61 0.97 0.6309 1% Metabolite-4611 50 0.9734 0.6318 0% Isobar-4-includes-Gluconic 61 0.9753 0.6318 1% acid-DL-arabinose-D- ribose-L-xylose- DL-lyxose-D-xylulose 1-methyladenine 50 0.979 0.633 2% 3-phospho-d-glycerate 61 0.9819 0.633 1% Metabolite-4365 50 0.9829 0.633 −1% Metabolite-4866 61 0.9912 0.6371 0% Metabolite-4003 61 0.9994 0.6412 0%

TABLE 2 Prostate Cancer Biomarkers from subjects with Metastatic, High Grade Prostate Cancer compared to Control subjects. % Change COMPOUND Library p-value q-value in PCA inosine 61 <0.0001 <0.0001 −269%  Metabolite - 2-Aminoethyl-phosphonate 61 <0.0001 <0.0001 −437%  Metabolite - 1597 61 <0.0001 <0.0001 110% Metabolite - 1498 61 <0.0001 <0.0001 188% octadecanoic acid 50 <0.0001 <0.0001 136% Metabolite - 3390 61 <0.0001 <0.0001 −330%  riboflavine 61 <0.0001 <0.0001 196% leucine 50 <0.0001 <0.0001 216% phosphate 50 <0.0001 <0.0001 150% anthranilic acid 50 <0.0001 <0.0001 140% glycerol 50 <0.0001 <0.0001 352% Metabolite - 3808 61 <0.0001 <0.0001 −452%  valine 50 <0.0001 <0.0001 103% Metabolite - 1595-possible-glutathione- 61 <0.0001 <0.0001 −695%  metabolite n-hexadecanoic acid 50 <0.0001 <0.0001 365% heptadecanoic acid 50 <0.0001 <0.0001 201% Metabolite - 3998 50 <0.0001 <0.0001 101% Metabolite - 1679 61 <0.0001 <0.0001 597% phenylalanine 61 <0.0001 <0.0001  93% Isobar-24-includes-L-arabitol-adonitol 61 <0.0001 <0.0001 313% Metabolite - 2292 61 <0.0001 <0.0001 −644%  tryptophan 61 <0.0001 <0.0001 112% Metabolite - 3893 61 <0.0001 <0.0001 −757%  xanthine 61 <0.0001 <0.0001 1072%  glycerate 61 <0.0001 <0.0001 375% Metabolite - 3178 61 <0.0001 <0.0001 −1223%  ribulose-5-phosphate 50 <0.0001 <0.0001 −272%  noradrenaline 50 <0.0001 <0.0001  88% Metabolite - 3085 50 <0.0001 <0.0001 −224%  Metabolite - 2272 61 <0.0001 <0.0001 594% Metabolite - 4013 61 <0.0001 <0.0001 443% taurine 61 <0.0001 <0.0001 −219%  uracil 50 <0.0001 <0.0001 933% Metabolite - 3165 61 <0.0001 <0.0001  75% Metabolite - 2973 50 <0.0001 <0.0001 −214%  histidine 50 <0.0001 <0.0001 120% adenosine 61 <0.0001 1.00E−04 −276%  9-12-octadecadienoic acid-z-z- 50 <0.0001 1.00E−04 518% isoleucine 50 <0.0001 1.00E−04  68% Metabolite - 3772 61 <0.0001 1.00E−04  83% DL-homocysteine 61 <0.0001 1.00E−04 216% pantothenic acid 61 <0.0001 1.00E−04 164% Metabolite - 3778 61 <0.0001 1.00E−04 −327%  Metabolite - 4611 50 <0.0001 1.00E−04 388% Isobar-6-includes-valine-betaine 61 <0.0001 1.00E−04  78% tetradecanoic acid 50 <0.0001 1.00E−04 810% Metabolite - 3810 61 <0.0001 1.00E−04 −261%  proline 50 <0.0001 1.00E−04 209% Metabolite - 1576 61 1.00E−04 1.00E−04 204% Metabolite - 5210 50 1.00E−04 1.00E−04 −231%  4-hydroxyphenylpyruvate 61 1.00E−04 1.00E−04 −423%  Metabolite - 3102 50 1.00E−04 1.00E−04 918% gamma-L-glutamyl-L-glutamine 61 1.00E−04 1.00E−04 −433%  Metabolite - 1977 61 1.00E−04 1.00E−04 382% palmitoleic acid 50 1.00E−04 1.00E−04 1547%  n-dodecanoate 50 1.00E−04 1.00E−04 418% Metabolite - 1114 61 1.00E−04 1.00E−04 106% Metabolite - 4617 61 1.00E−04 1.00E−04 217% Metabolite - 5107 61 1.00E−04 1.00E−04 268% L-allo-threonine 50 1.00E−04 2.00E−04  86% threonine 50 1.00E−04 2.00E−04  88% Metabolite - 3138 61 1.00E−04 2.00E−04 268% tyrosine 61 1.00E−04 2.00E−04  68% Metabolite - 1349 61 1.00E−04 2.00E−04 −885%  arachidonic acid 50 1.00E−04 2.00E−04 164% Metabolite - 4046 50 1.00E−04 2.00E−04 3090%  Metabolite - 4620 61 1.00E−04 2.00E−04 854% Metabolite - 4075 50 1.00E−04 2.00E−04 971% urea 50 2.00E−04 2.00E−04 234% Metabolite - 2181 61 2.00E−04 2.00E−04 189% Metabolite - 5209 50 2.00E−04 2.00E−04 −539%  Metabolite - 2108 61 2.00E−04 2.00E−04 155% Metabolite - 1351 61 2.00E−04 2.00E−04 366% glycine 50 2.00E−04 2.00E−04 101% Metabolite - 3003 50 2.00E−04 2.00E−04 122% Metabolite - 4134 50 2.00E−04 2.00E−04 458% Metabolite - 1329 61 2.00E−04 2.00E−04 171% Metabolite - 1394-possible-Losartan 61 2.00E−04 2.00E−04 158% Metabolite - 3014 50 2.00E−04 2.00E−04 327% Metabolite - 1116 61 3.00E−04 3.00E−04 446% Metabolite - 5212 50 3.00E−04 3.00E−04 −488%  Metabolite - 1465 61 3.00E−04 3.00E−04 512% Metabolite - 5228 50 3.00E−04 3.00E−04  86% Isobar-2-includes-3-amino-isobutyrate- 61 3.00E−04 3.00E−04 249% 2-amino-butyrate-4-aminobutanoic acid- dimethylglycine-choline- glutathione-reduced 61 3.00E−04 3.00E−04 −819%  1-7-dihydro-6h-purin-6-one 61 3.00E−04 3.00E−04  63% Metabolite - 2924 50 3.00E−04 3.00E−04 332% methionine 61 3.00E−04 3.00E−04  68% Metabolite - 4649 61 4.00E−04 3.00E−04 224% fumaric acid 50 4.00E−04 3.00E−04 196% Metabolite - 1593 61 4.00E−04 4.00E−04 −409%  inositol-1-phosphate 50 4.00E−04 4.00E−04 163% Metabolite - 4051 50 4.00E−04 4.00E−04 663% lactate 50 4.00E−04 4.00E−04  49% Metabolite - 4117-possible-propranolol- 61 4.00E−04 4.00E−04 267% or-2-heptyl-3-hydroxy-quinolone N-N-dimethylarginine 61 4.00E−04 4.00E−04 267% Metabolite - 3370 61 4.00E−04 4.00E−04  79% citric acid 50 5.00E−04 4.00E−04 −1943%  glyceric acid 50 5.00E−04 4.00E−04 125% Metabolite - 3215 61 5.00E−04 4.00E−04 110% 1-methyladenosine 61 6.00E−04 5.00E−04 620% 5-hydroxyindoleacetate 50 6.00E−04 5.00E−04 −319%  S-5-adenosyl-L-methionine 61 6.00E−04 5.00E−04 230% catechol 61 7.00E−04 5.00E−04 595% Metabolite - 5110 61 7.00E−04 5.00E−04 278% Metabolite - 1069-possible- 61 7.00E−04 5.00E−04 −379%  dehydroepiandrosterone-sulfate- Metabolite - 4593 50 7.00E−04 5.00E−04 113% elaidic acid 50 7.00E−04 5.00E−04 526% Metabolite - 3833 61 7.00E−04 6.00E−04 247% Metabolite - 2711 61 8.00E−04 6.00E−04  84% carnitine 61 8.00E−04 6.00E−04 155% D-allose 50 8.00E−04 6.00E−04 −1265%  Metabolite - 3094 50 9.00E−04 6.00E−04  49% Metabolite - 5108 61 9.00E−04 6.00E−04 237% Metabolite - 3064 61 9.00E−04 6.00E−04 195% L-alpha-glycerophosphorylcholine 61 9.00E−04 6.00E−04 361% Metabolite - 5128 61 9.00E−04 6.00E−04 −2480%  Metabolite - 2567 61 9.00E−04 6.00E−04 132% uric acid 61 9.00E−04 7.00E−04 142% quinolinic acid 61 0.001 7.00E−04 173% Metabolite - 4518 50 0.001 7.00E−04 618% Metabolite - 4428 61 0.001 7.00E−04 210% Metabolite - 5214 50 0.0011 7.00E−04 −421%  Metabolite - 3044 61 0.0011 7.00E−04 187% Metabolite - 3816 61 0.0011 7.00E−04 −2267%  Metabolite - 1831-possible-Cl-adduct- 61 0.0011 7.00E−04 142% of-citrulline guanosine 61 0.0012 7.00E−04 −191%  3-methyl-L-histidine 61 0.0012 8.00E−04  83% Metabolite - 1843 61 0.0012 8.00E−04 524% cysteine 50 0.0012 8.00E−04 988% Metabolite - 5187 61 0.0012 8.00E−04 354% ethylmalonic acid 61 0.0012 8.00E−04 1277%  Metabolite - 2766 61 0.0012 8.00E−04 −2129%  Metabolite - 1104 61 0.0014 8.00E−04 −200%  3-methoxy-L-tyrosine 50 0.0014 9.00E−04 −570%  Metabolite - 3807 61 0.0014 9.00E−04 346% DL-pipecolic acid 61 0.0015 9.00E−04 296% Metabolite - 2041 61 0.0015 9.00E−04 198% malic acid 50 0.0015 9.00E−04  88% Metabolite - 4331 61 0.0016 9.00E−04  97% Metabolite - 5166 61 0.0017 0.001 157% Metabolite - 2111 61 0.0018 0.001 134% Metabolite - 5167 61 0.0018 0.001 146% Metabolite - 2867 61 0.0018 0.001 −6300%  3-phospho-d-glycerate 61 0.0018 0.001 −210%  Metabolite - 2109 61 0.0019 0.001 179% Metabolite - 5232 50 0.0019 0.0011 422% D-ribose 50 0.002 0.0011 −466%  Metabolite - 3771 61 0.002 0.0011 −163%  alanine 50 0.002 0.0011  86% Metabolite - 2753 61 0.002 0.0011 227% xanthosine 50 0.002 0.0011 −391%  arabinose 50 0.002 0.0011 −437%  Metabolite - 1323-possible-4- 61 0.0021 0.0011 311% sulfobenzyl-alcohol Metabolite - 3489 61 0.0021 0.0011 −552%  trans-4-hydroxyproline 50 0.0022 0.0011 208% Metabolite - 3966 61 0.0022 0.0012 159% Metabolite - 1713 61 0.0025 0.0013 212% Metabolite - 2237 61 0.0026 0.0013 307% Metabolite - 2548 61 0.0026 0.0013  97% Metabolite - 3364 61 0.0026 0.0013 272% melatonin 50 0.0026 0.0013 227% Isobar-5-includes-asparagine-ornithine 61 0.0028 0.0014 105% Metabolite - 1819 61 0.0029 0.0014  69% inositol 50 0.0029 0.0014 −541%  spermine 50 0.0029 0.0014 −5110%  Metabolite - 1288 61 0.003 0.0014 221% Metabolite - 5109 61 0.0031 0.0015 385% thymine 50 0.0031 0.0015 561% Isobar-19-includes-D-saccharic acid-2- 61 0.0031 0.0015 −213%  deoxy-D-galactose-2-deoxy-D-glucose- L-fucose-L-rhamnose Metabolite - 2141 61 0.0032 0.0015 263% Metabolite - 1327-possible-bilirubin 61 0.0033 0.0015  84% Metabolite - 2900- 61 0.0034 0.0016 152% alpha-4-dihydroxybenzenepropanoic 50 0.0034 0.0016 2300%  acid Metabolite - 3183-possible-gamma-L- 61 0.0035 0.0016 241% glutamyl-L-phenylalanine-or-aspartame glutamic acid 50 0.0036 0.0017 114% 5-s-methyl-5-thioadenosine 61 0.0036 0.0017 235% 2-deoxy-D-ribose 61 0.0037 0.0017  68% 4-hydroxy-2-quinolinecarboxylic acid 61 0.0037 0.0017  81% Metabolite - 4869 61 0.0038 0.0017 184% Metabolite - 4015 50 0.0038 0.0017 247% N-acetylserotonin 50 0.0038 0.0017 1007%  allantoin 61 0.0039 0.0018 164% Metabolite - 2118 61 0.0041 0.0018 −138%  Metabolite - 2323 61 0.0041 0.0018 111% Isobar-22-includes-glutamic acid-O- 61 0.0041 0.0018  52% acetyl-L-serine mercaptopyruvate 61 0.0043 0.0019 130% 3-methylglutaric acid 61 0.0044 0.0019 319% Metabolite - 2139 61 0.0045 0.0019 165% Spermidine 50 0.0045 0.0019 −2575%  Metabolite - 3974 61 0.0045 0.0019 114% azelaic acid 61 0.0045 0.0019  90% Metabolite - 5186 61 0.0048 0.002 6750%  4-acetamidobutyric acid 61 0.0048 0.002 754% Metabolite - 5215 50 0.0049 0.002 −151%  dethiobiotin 50 0.0049 0.002  45% Metabolite - 1496 61 0.0049 0.002  46% Metabolite - 3955 61 0.0049 0.002 −138%  2-keto-L-gulonic acid 50 0.0054 0.0022 −167%  Metabolite - 5170 61 0.0055 0.0022 −600%  Metabolite - 2466 61 0.006 0.0024 −194%  caffeine 61 0.0064 0.0026 −287%  Isobar-40-includes-Maltotetraose- 61 0.0066 0.0027 −264%  stachyose Metabolite - 1211 61 0.0069 0.0027 −188%  Metabolite - 4706 61 0.0069 0.0027 304% Metabolite - 4027 50 0.0069 0.0027 520% Metabolite - 4150 50 0.007 0.0028 −483%  4-methyl-2-oxopentanoate 61 0.0072 0.0028 194% Metabolite - 1216 61 0.0073 0.0028 109% Metabolite - 3837 61 0.0074 0.0029 210% S-adenosyl-l-homocysteine 61 0.0075 0.0029  72% Metabolite - 2768 61 0.0077 0.0029 −800%  suberic acid 61 0.008 0.0031 106% Metabolite - 3554 61 0.0081 0.0031 272% pyrophosphate 50 0.0081 0.0031 120% Metabolite - 3996 50 0.0081 0.0031  86% 3-hydroxy-3-methylglutarate 50 0.0084 0.0031 123% Metabolite - 4615 61 0.0084 0.0031 120% 4-Guanidinobutanoic acid 61 0.0084 0.0031 110% Metabolite - 2348 61 0.0088 0.0032 151% Metabolite - 1980 61 0.0088 0.0032 138% N-5-aminocarbonyl-L-ornithine 50 0.0089 0.0033 177% Metabolite - 3997 61 0.009 0.0033 1100%  fructose 50 0.0093 0.0034 −336%  Metabolite - 1286 61 0.0093 0.0034 −127%  Metabolite - 1342-possible- 61 0.0094 0.0034 229% phenylacetylglutamine Metabolite - 4866 61 0.0099 0.0036 −479%  Metabolite - 3020 50 0.0103 0.0037  97% Metabolite - 2607 61 0.0103 0.0037 147% Metabolite - 1609 61 0.0104 0.0037 −227%  Metabolite - 4516 50 0.0115 0.0041 −213%  1-methyladenine 50 0.0116 0.0041 −580%  Metabolite - 2232 61 0.0116 0.0041 −242%  picolinic acid 61 0.0118 0.0041 137% Metabolite - 2774 61 0.0126 0.0044  96% Metabolite - 2690 61 0.0127 0.0044 2080%  Metabolite - 3221 61 0.0128 0.0044 107% Isobar-30-includes-maltotetraose- 61 0.0132 0.0045 −202%  stachyose Metabolite - 3180 61 0.0134 0.0046 174% Metabolite - 3220 61 0.0134 0.0046 267% Metabolite - 3752 61 0.0135 0.0046 1122%  Metabolite - 4787 61 0.0135 0.0046 −540%  Metabolite - 4365 50 0.0146 0.0049 −229%  Metabolite - 3957 61 0.0147 0.0049  59% DL-cystathionine 50 0.0148 0.0049 271% 2-deoxyuridine 61 0.0149 0.0049 128% Metabolite - 3379 61 0.0151 0.005 −138%  sarcosine 50 0.0153 0.005 2138%  Metabolite - 4018 61 0.0155 0.0051 813% cholesterol 50 0.0162 0.0053  33% 5-6-dihydrouracil 61 0.017 0.0055 154% 5-oxoproline 50 0.0174 0.0057  55% 3-amino-isobutyrate 50 0.0177 0.0057 1561%  Metabolite - 1961-retired-glycocholic 61 0.0179 0.0058 691% acid Metabolite - 4043 50 0.0185 0.0059  38% Metabolite - 2981 50 0.0186 0.0059  27% Metabolite - 3984 61 0.0186 0.0059 800% tyramine 50 0.0186 0.0059  38% Metabolite - 3526 61 0.0194 0.0061 127% Metabolite - 4168 61 0.0198 0.0062  54% Putrescine 50 0.0199 0.0062 −2967%  Metabolite - 2099 61 0.0204 0.0064 −247%  pyridoxamine-phosphate 61 0.0205 0.0064 −197%  sn-Glycerol-3-phosphate 50 0.0214 0.0066 738% GABA 50 0.022 0.0068 −192%  Metabolite - 4362 50 0.0223 0.0069 −215%  uridine-5-diphosphoglucose 50 0.0226 0.0069 −142%  saccharopine 61 0.0231 0.007  81% Metabolite - 3132 61 0.0231 0.007 −195%  Metabolite - 4550 61 0.0234 0.0071  92% asparagine 50 0.0235 0.0071  81% Metabolite - 2143 61 0.0238 0.0071 345% Metabolite - 1970 61 0.0253 0.0076 141% L-kynurenine 50 0.0258 0.0077 335% Metabolite - 1129 61 0.0259 0.0077 −183%  Metabolite - 1333 61 0.0263 0.0078 −268%  Metabolite - 2406 61 0.0264 0.0078 238% Metabolite - 4632 50 0.0266 0.0078  53% Metabolite - 3123 61 0.0267 0.0078  87% Metabolite - 1911 61 0.0274 0.008 100% Metabolite - 2806 61 0.0277 0.0081 −154%  Metabolite - 4014 50 0.0292 0.0085  69% Metabolite - 1608 61 0.0295 0.0085 −605%  Metabolite - 1974 61 0.0295 0.0085 218% Metabolite - 3708 61 0.0297 0.0085  46% Metabolite - 3896 61 0.0297 0.0085 360% Metabolite - 1303 61 0.0303 0.0086 −256%  Metabolite - 2212 61 0.0308 0.0087 453% glutarate 61 0.0309 0.0087 155% Metabolite - 3436 61 0.0316 0.0089 148% D-sorbitol-6-phosphate 50 0.0319 0.009 −190%  Metabolite - 3430 61 0.0324 0.0091 110% Metabolite - 3992- 61 0.033 0.0092 −132%  Isobar-1-includes-mannose-fructose- 61 0.034 0.0094 −187%  glucose-galactose-alpha-L- sorbopyranose-inositol-D-allose Metabolite - 2390 61 0.0345 0.0096 343% Metabolite - 3002 50 0.0346 0.0096  33% Metabolite - 3545 61 0.0354 0.0097 165% Metabolite - 1186 61 0.0373 0.0102 −1210%  Metabolite - 1111-possible- 61 0.0375 0.0103  50% methylnitronitrosoguanidine-or-ethyl- thiocarbamoylacetate Metabolite - 5207 50 0.0377 0.0103 −136%  Metabolite - 3016 50 0.0379 0.0103 −184%  Metabolite - 1963 61 0.0393 0.0106 −151%  xylitol 50 0.0393 0.0106 123% Metabolite - 3022 50 0.0396 0.0107  92% Metabolite - 2897 61 0.0416 0.0112  92% uridine-5-monophosphate 61 0.0433 0.0116 −161%  Metabolite - 2027 61 0.044 0.0117 490% 2-deoxyuridine-5-triphosphate 61 0.0444 0.0118 −174%  Metabolite - 3034 50 0.0448 0.0119  67% 3-hydroxybutanoic acid 50 0.0476 0.0125 218% 3-methyl-2-oxovaleric acid 61 0.0477 0.0125 241% Metabolite - 3980 61 0.0484 0.0127 −169%  niacinamide 61 0.052 0.0135 −138%  Isobar-27-includes-L-kynurenine-alpha- 61 0.052 0.0135 645% 2-diamino-gamma-oxobenzenebutanoic acid Metabolite - 4133 50 0.0522 0.0135 120% Metabolite - 2827 61 0.0526 0.0136 −202%  Metabolite - 5189 61 0.0536 0.0138 110% Metabolite - 2778 61 0.0537 0.0138 269% Metabolite - 3027 50 0.0537 0.0138 130% biliverdin 61 0.0539 0.0138 −151%  Metabolite - 3813 61 0.0547 0.0139 146% uridine-5-diphosphoglucuronic acid 50 0.055 0.014 129% Metabolite - 3951 61 0.0551 0.014  64% phytonadione 50 0.0554 0.014  29% Metabolite - 3139 61 0.0565 0.0142  71% Metabolite - 3176-possible-creatine 61 0.0567 0.0142 −129%  Metabolite - 1718 61 0.0568 0.0142 −169%  Metabolite - 3783 61 0.0574 0.0143 −174%  Metabolite - 4616 61 0.0585 0.0145 210% sorbitol 50 0.0592 0.0147 841% Metabolite - 2064 61 0.0623 0.0154  54% cytidine 61 0.0628 0.0155 181% Metabolite - 5126 61 0.0633 0.0156 −145%  beta-alanine 50 0.0642 0.0157  52% Metabolite - 4567 61 0.066 0.0161 632% glucarate 50 0.0686 0.0166 −326%  Metabolite - 3539 61 0.0687 0.0166 143% Metabolite - 3056 61 0.0688 0.0166 1354%  Metabolite - 2072 61 0.0689 0.0166 368% Metabolite - 4032 50 0.0745 0.0179 162% Metabolite - 5229 50 0.0758 0.0182 −144%  beta-nicotinamide-adenine-dinucleotide 61 0.0761 0.0182 2363%  Metabolite - 3960 61 0.0763 0.0182 −140%  Metabolite - 2121 61 0.0771 0.0183  98% Metabolite - 3238 61 0.0778 0.0185 247% Metabolite - 3129 61 0.0848 0.02 −118%  25-hydroxycholesterol 50 0.0913 0.0215  11% Metabolite - 5226 50 0.0977 0.023 100% Metabolite - 1575 61 0.1 0.0234 −188%  3-nitro-L-tyrosine 50 0.1027 0.024  96% Metabolite - 1142-possible-5- 61 0.104 0.0242 428% hydroxypentanoate-or-beta- hydroxyisovaleric acid Metabolite - 2250 61 0.1053 0.0245 −150%  gamma-glu-cys 61 0.1128 0.0261 −229%  Metabolite - 2853 61 0.1138 0.0263  79% Metabolite - 3756 61 0.1144 0.0264 593% Metabolite - 2368 61 0.115 0.0264 −507%  o-phosphoethanolamine 50 0.1171 0.0268  70% Metabolite - 1497 61 0.1186 0.0271 −135%  Metabolite - 3475 61 0.1222 0.0278 −148%  Metabolite - 2185 61 0.1229 0.0279  61% alpha-L-sorbopyranose 50 0.1302 0.0295 −139%  Metabolite - 4512 50 0.1307 0.0295 −167%  histamine 61 0.1324 0.0298 −130%  Metabolite - 1085-possible-isolobinine- 61 0.135 0.0303  34% or-4-aminoestra-1-3-5-10-triene-3- 17beta-diol Isobar-18-includes-D-fructose-1- 61 0.1383 0.0309  52% phosphate-beta-D-fructose-6- phosphate Metabolite - 2824 61 0.1384 0.0309 125% Metabolite - 3848 61 0.1384 0.0309  55% biotin 61 0.1397 0.0311 −169%  L-homoserine-lactone 61 0.1404 0.0311 −118%  cytidine-5-monophosphate 61 0.1411 0.0312  41% Metabolite - 3952 61 0.1461 0.0322 −144%  Metabolite - 3576 61 0.1462 0.0322  26% Metabolite - 2821 61 0.1476 0.0324 291% Metabolite - 2255 61 0.1483 0.0324 −169%  mannose 50 0.1503 0.0328 −147%  alpha-amino-adipate 50 0.1518 0.033 113% Metabolite - 3696-retired-isobar- 61 0.152 0.033 230% glycocheBenignoxycholic acid- glycodeoxycholic acid glucose-6-phosphate 50 0.153 0.0331 −144%  Metabolite - 2724 61 0.1543 0.0333  51% Metabolite - 1616 61 0.1552 0.0334 −158%  Metabolite - 2347 61 0.1567 0.0337  42% Metabolite - 2313 61 0.1604 0.0344  22% Metabolite - 2389 61 0.1657 0.0354  22% mannose-6-phosphate 50 0.1678 0.0357 −200%  Metabolite - 4503 50 0.1692 0.036 426% serine 50 0.1707 0.0362  22% Metabolite - 2005 61 0.172 0.0364  52% Metabolite - 4806 50 0.1728 0.0364  22% Metabolite - 4030-possible- 61 0.1763 0.0371  52% glutethimide-or-securinine Metabolite - 3832-possible-phenol- 61 0.1775 0.0372 199% sulfate glucono-gamma-lactone 50 0.1776 0.0372 −143%  Metabolite - 1070 61 0.1836 0.0383  23% Metabolite - 4019 50 0.1844 0.0384  32% Metabolite - 4355 50 0.1854 0.0385  22% N-acetyl-D-glucosamine 50 0.186 0.0385  31% Metabolite - 2198 61 0.1861 0.0385 −144%  Metabolite - 4053 50 0.1928 0.0397  45% Isobar-3-includes-inositol-1-phosphate- 61 0.1941 0.0399 −127%  mannose-6-phosphate-glucose-6- phosphate-D-mannose-1-phosphate- alpha-D-glucose-1-phosphate-alpha-D- galactose-1-phosphate maltose 50 0.1978 0.0406 426% Metabolite - 4868-possible-Bradykinin 61 0.1998 0.0409 −120%  Metabolite - 4497 50 0.2013 0.0411 −132%  Isobar-4-includes-Gluconic acid-DL- 61 0.2053 0.0418 −129%  arabinose-D-ribose-L-xylose-DL-lyxose- D-xylulose Metabolite - 1457 61 0.2062 0.0419  41% Metabolite - 2691 61 0.2102 0.0426  48% Metabolite - 2075 61 0.2122 0.0428 −161%  dulcitol 50 0.2122 0.0428 −146%  Metabolite - 4931 61 0.2153 0.0433  30% orotidine-5-phosphate 61 0.2187 0.0439  81% Metabolite - 3074 50 0.2196 0.0439  86% hypotaurine 50 0.221 0.044  60% N-acetyl-D-galactosamine 50 0.2211 0.044  33% Metabolite - 4116 61 0.2274 0.0452  16% Metabolite - 3476 61 0.2356 0.0467 −137%  adenine 50 0.2383 0.0471  58% N-6-trimethyl-l-lysine 61 0.2389 0.0471 −132%  2-deoxy-D-glucose 50 0.2399 0.0472 −142%  Metabolite - 3317 61 0.2414 0.0474  42% glutamine 50 0.2426 0.0475  55% Metabolite - 1573 61 0.247 0.0482  42% Isobar-32-includes-N-acetyl-D- 61 0.2551 0.0497  24% glucosamine-N-acetyl-D-mannosamine Metabolite - 1248-possible-avermectin- 61 0.2596 0.0505 −135%  aglycone Metabolite - 2388 61 0.2679 0.052  19% Metabolite - 2546 61 0.2715 0.0525  91% Metabolite - 1113-possible- 61 0.28 0.054  23% acetylcarnitine-or-isopentyl-adenine alpha-D-ribose-5-phosphate 50 0.2826 0.0544 −129%  Metabolite - 5227 50 0.2888 0.0555 1117%  Metabolite - 3534 61 0.2998 0.0575  58% 2-acetamido-1-amino-1-2-dideoxy-beta- 50 0.3037 0.0581  25% D-glucopyranose Metabolite - 5211 50 0.3045 0.0581 −184%  Metabolite - 1653 61 0.3126 0.0595 −127%  Metabolite - 2036 61 0.3211 0.061 115% Metabolite - 4003 61 0.3245 0.0615 −124%  Metabolite - 4058 50 0.3276 0.0619  27% Metabolite - 2055 61 0.3345 0.0631 −122%  3-phospho-l-serine 61 0.3436 0.0646 −109%  Metabolite - 3073 50 0.3654 0.0686  45% Metabolite - 4272 50 0.3717 0.0696 −119%  Metabolite - 1203-possible- 61 0.3764 0.0703 −140%  acetylbrowniine-tricornine-germine-or- veracevine Metabolite - 4448 61 0.4001 0.0746 −116%  Metabolite - 2846 61 0.4122 0.0767  80% Metabolite - 5213 50 0.4147 0.077 −118%  galactose 50 0.4216 0.0781  17% hippuric acid 61 0.4252 0.0786 −139%  Metabolite - 3514-retired-topiramate 61 0.4327 0.0798 −341%  Isobar-21-includes-gamma- 61 0.4395 0.0808  37% aminobutyryl-L-histidine-L-anserine uridine 61 0.4426 0.0812 −108%  Metabolite - 1330 61 0.4441 0.0813  57% Metabolite - 3994 61 0.4454 0.0814 −128%  Metabolite - 4017 50 0.4508 0.0822  16% Metabolite - 5147 61 0.461 0.0838 196% Metabolite - 4637 50 0.4761 0.0864  12% Metabolite - 3668 61 0.477 0.0864  23% Metabolite - 3365 61 0.4807 0.0868 −127%  Metabolite - 1455 61 0.4815 0.0868 −171%  Metabolite - 4096-gamma-glu-gly-leu- 61 0.4858 0.0874 −109%  Metabolite - 1187 61 0.4937 0.0886 −126%  Metabolite - 2194 61 0.5147 0.0922  30% Metabolite - 3543 61 0.5175 0.0925  44% possible-ISOBAR-DL-aspartic acid- 50 0.519 0.0925  22% Metabolite - 3522 61 0.5205 0.0925 −231%  Metabolite - 2105 61 0.521 0.0925  15% dihydroxyacetone-phosphate 61 0.532 0.0942 −116%  Metabolite - 3051 61 0.5346 0.0945  29% Metabolite - 3755 61 0.5481 0.0967  12% Metabolite - 3604 61 0.5512 0.097 −129%  Metabolite - 4238 61 0.5582 0.098  23% Metabolite - 4523 50 0.5592 0.098  13% Metabolite - 2407 61 0.5602 0.098 −133%  Metabolite - 4354 50 0.5857 0.1021 −112%  Metabolite - 2129 61 0.5869 0.1021  17% Metabolite - 4002 50 0.5873 0.1021  13% Metabolite - 1392 61 0.5947 0.1031  36% 6-phosphogluconic acid 61 0.5976 0.1034  8% phosphoenolpyruvate 50 0.6017 0.1039  16% Carnosine 61 0.6048 0.1042 −112%  alpha-keto-glutarate 61 0.6073 0.1044  47% Metabolite - 3484 61 0.6115 0.1049  27% Metabolite - 2279 61 0.6193 0.1061 −115%  Metabolite - 2074 61 0.6245 0.1067 −118%  Isobar-31-includes-maltotriose- 61 0.6315 0.1077 −115%  melezitose N-acetylneuraminate 61 0.639 0.1088  11% ascorbic acid 50 0.6412 0.1089  18% Metabolite - 4084 50 0.6527 0.1106 −104%  Metabolite - 1088 61 0.6537 0.1106  16% Metabolite - 4020 50 0.6567 0.1108  12% creatinine 61 0.6624 0.1116  6% Metabolite - 2174 61 0.6687 0.1124  15% Metabolite - 3498 61 0.6739 0.1129  12% Metabolite - 3401 61 0.6742 0.1129  16% succinate 50 0.6845 0.1144 −120%  L-histidinol 61 0.6889 0.1149 −108%  Metabolite - 4667 61 0.6922 0.1152 −109%  Metabolite - 4510 50 0.6961 0.1156  9% Metabolite - 3099 50 0.6979 0.1157  7% Metabolite - 2056 61 0.7004 0.1158 −106%  Metabolite - 1183 61 0.7152 0.118 −118%  beta-D-lactose 50 0.7236 0.1192  14% Metabolite - 4796 50 0.7305 0.1201  14% Metabolite - 4235 61 0.7549 0.1238 −126%  Metabolite - 4514 50 0.7707 0.1262 −105%  Metabolite - 3531 61 0.7817 0.1277 −126%  Metabolite - 5089 61 0.783 0.1277 −121%  benzoic acid 50 0.797 0.1297 −103%  Isobar-17-includes-arginine-N-alpha- 61 0.808 0.1312  6% acetyl-ornithine ornithine 50 0.8172 0.1325 −106%  Metabolite - 2319 61 0.8211 0.1328 −111%  cystine 50 0.8239 0.1329 −104%  Metabolite - 5086 61 0.8247 0.1329 −110%  Metabolite - 3189 61 0.8275 0.1331  7% guanine 50 0.8461 0.1356  8% Metabolite - 3517 61 0.8465 0.1356  9% Metabolite - 3040 50 0.8495 0.1358 −104%  Metabolite - 2558 61 0.8782 0.1401  7% Metabolite - 2180 61 0.8839 0.1408 −104%  arginino-succinate 61 0.8933 0.1419 −103%  Metabolite - 4080 50 0.8946 0.1419  4% Metabolite - 2688 61 0.9212 0.1459 −101%  aspartate 61 0.9268 0.1463  2% Metabolite - 2703 61 0.9278 0.1463 −103%  Metabolite - 5087 61 0.9434 0.1485 −104%  Metabolite - 3694 61 0.9614 0.1509 −102%  Metabolite - 1979-Cl-adduct-of- 61 0.9624 0.1509 −101%  C6H10O5 Metabolite - 1975 61 0.9674 0.1514  1% Metabolite - 4271 50 0.9952 0.1555  1%

TABLE 3 Prostate Cancer Biomarkers from subjects with Metastatic, High Grade Prostate Cancer compared to subjects with Lower Grade Prostate Cancer. % Change in high COMPOUND Library p-value q-value grade PCA inosine 61 <0.0001 <0.0001 −63% Metabolite - 3390 61 <0.0001 <0.0001 −69% octadecanoic acid 50 <0.0001 <0.0001  89% Metabolite - 2-Aminoethyl-phosphonate 61 <0.0001 <0.0001 −78% glycerol 50 <0.0001 <0.0001 200% n-hexadecanoic acid 50 <0.0001 <0.0001 241% leucine 50 <0.0001 1.00E−04  96% Metabolite - 1498 61 <0.0001 2.00E−04  95% Metabolite - 4013 61 <0.0001 2.00E−04 418% Isobar-24-includes-L-arabitol-adonitol 61 <0.0001 2.00E−04 183% 4-hydroxyphenylpyruvate 61 <0.0001 2.00E−04 −71% Putrescine 50 <0.0001 2.00E−04 −81% glycerate 61 <0.0001 2.00E−04 235% xanthine 61 <0.0001 2.00E−04 331% uridine 61 <0.0001 2.00E−04 −46% Metabolite - 1597 61 <0.0001 3.00E−04  63% Metabolite - 4611 50 <0.0001 3.00E−04 388% uracil 50 1.00E−04 5.00E−04 351% 3-phospho-d-glycerate 61 1.00E−04 6.00E−04 −53% tetradecanoic acid 50 1.00E−04 6.00E−04 472% Metabolite - 3102 50 1.00E−04 6.00E−04 716% inositol 50 1.00E−04 6.00E−04 −74% riboflavine 61 1.00E−04 6.00E−04  77% Metabolite - 3772 61 1.00E−04 6.00E−04  76% Metabolite - 3085 50 1.00E−04 6.00E−04 −53% 9-12-octadecadienoic acid-z-z- 50 1.00E−04 6.00E−04 256% anthranilic acid 50 1.00E−04 6.00E−04  57% n-dodecanoate 50 1.00E−04 6.00E−04 351% Metabolite - 3215 61 1.00E−04 6.00E−04 157% palmitoleic acid 50 1.00E−04 6.00E−04 825% urea 50 1.00E−04 6.00E−04 241% Metabolite - 4620 61 1.00E−04 6.00E−04 854% valine 50 2.00E−04 7.00E−04  47% Metabolite - 2466 61 2.00E−04 7.00E−04 −74% Metabolite - 1349 61 2.00E−04 7.00E−04 −88% Metabolite - 4075 50 2.00E−04 8.00E−04 689% Metabolite - 3808 61 2.00E−04 8.00E−04 −72% Metabolite - 5209 50 2.00E−04 8.00E−04 −78% Metabolite - 4134 50 2.00E−04 8.00E−04 444% Metabolite - 1116 61 2.00E−04 8.00E−04 476% Metabolite - 4150 50 2.00E−04 8.00E−04 −68% Metabolite - 3014 50 3.00E−04 9.00E−04 322% Metabolite - 2548 61 3.00E−04 9.00E−04 189% Metabolite - 3178 61 3.00E−04 0.001 −88% uric acid 61 3.00E−04 0.001 200% Metabolite - 1351 61 3.00E−04 0.001 291% Metabolite - 4046 50 3.00E−04 0.001 688% Metabolite - 1329 61 4.00E−04 0.001 147% Metabolite - 4649 61 4.00E−04 0.0011 224% Metabolite - 3992- 61 4.00E−04 0.0011 −33% Metabolite - 2272 61 4.00E−04 0.0012 176% Metabolite - 2181 61 5.00E−04 0.0013 135% Metabolite - 4869 61 5.00E−04 0.0013 361% arabinose 50 5.00E−04 0.0013 −73% D-ribose 50 5.00E−04 0.0013 −74% xanthosine 50 5.00E−04 0.0013 −69% Isobar-19-includes-D-saccharic acid-2- 61 6.00E−04 0.0015 −53% deoxy-D-galactose-2-deoxy-D-glucose-L- fucose-L-rhamnose 3-methoxy-L-tyrosine 50 6.00E−04 0.0015 −75% 2-keto-L-gulonic acid 50 6.00E−04 0.0015 −47% Metabolite - 2766 61 7.00E−04 0.0015 −93% lactate 50 7.00E−04 0.0017  38% Metabolite - 3893 61 7.00E−04 0.0017 −85% tryptophan 61 8.00E−04 0.0017  53% phenylalanine 61 8.00E−04 0.0017  43% Metabolite - 1819 61 8.00E−04 0.0018  99% Metabolite - 1323-possible-4-sulfobenzyl- 61 9.00E−04 0.0019 495% alcohol Metabolite - 3123 61 0.001 0.0021 223% guanosine 61 0.001 0.0022 −48% proline 50 0.0011 0.0022  94% N-acetyl-D-galactosamine 50 0.0011 0.0022 −58% Metabolite - 2255 61 0.0011 0.0022 −59% pantothenic acid 61 0.0011 0.0022  89% Metabolite - 5212 50 0.0011 0.0022 −80% DL-pipecolic acid 61 0.0012 0.0023 329% Metabolite - 5187 61 0.0012 0.0023 348% Metabolite - 5207 50 0.0012 0.0023 −35% creatinine 61 0.0013 0.0023  41% Metabolite - 4518 50 0.0013 0.0024 490% Metabolite - 3807 61 0.0013 0.0024 363% Metabolite - 2924 50 0.0014 0.0024 187% Metabolite - 4617 61 0.0014 0.0024 107% Metabolite - 3165 61 0.0014 0.0024  39% Metabolite - 4428 61 0.0014 0.0024 155% Metabolite - 5107 61 0.0014 0.0024 170% Metabolite - 3833 61 0.0015 0.0024 188% Metabolite - 2607 61 0.0015 0.0024 348% ethylmalonic acid 61 0.0015 0.0024 894% Metabolite - 3837 61 0.0017 0.0028 363% Metabolite - 3132 61 0.0017 0.0028 −58% Metabolite - 4051 50 0.0018 0.0028 266% Isobar-3-includes-inositol-1-phosphate- 61 0.0018 0.0029 −52% mannose-6-phosphate-glucose-6- phosphate-D-mannose-1-phosphate-alpha- D-glucose-1-phosphate-alpha-D-galactose- 1-phosphate Metabolite - 3003 50 0.0019 0.0029  94% allantoin 61 0.002 0.0031 211% Metabolite - 3138 61 0.0021 0.0032 128% catechol 61 0.0021 0.0032 292% heptadecanoic acid 50 0.0022 0.0032  71% Metabolite - 1843 61 0.0022 0.0033 361% N-6-trimethyl-l-lysine 61 0.0023 0.0033 −44% phosphate 50 0.0024 0.0034  49% Isobar-2-includes-3-amino-isobutyrate-2- 61 0.0025 0.0035 121% amino-butyrate-4-aminobutanoic acid- dimethylglycine-choline- niacinamide 61 0.0025 0.0036 −35% Metabolite - 2237 61 0.0026 0.0037 291% Metabolite - 2567 61 0.0028 0.0038 101% S-5-adenosyl-L-methionine 61 0.0028 0.0038 153% Metabolite - 3489 61 0.0028 0.0038 −81% Metabolite - 2867 61 0.0029 0.0039 −99% 1-methyladenosine 61 0.0029 0.0039 260% thymine 50 0.0031 0.0041 561% Metabolite - 3543 61 0.0032 0.0041 174% D-sorbitol-6-phosphate 50 0.0032 0.0041 −66% Metabolite - 2250 61 0.0033 0.0042 −61% citric acid 50 0.0034 0.0043 −92% L-alpha-glycerophosphorylcholine 61 0.0035 0.0043 192% Metabolite - 1288 61 0.0035 0.0043 212% elaidic acid 50 0.0035 0.0044 241% alpha-4-dihydroxybenzenepropanoic acid 50 0.0036 0.0044 1900%  Metabolite - 3996 50 0.0036 0.0044 104% Metabolite - 2711 61 0.0037 0.0044  65% mannose-6-phosphate 50 0.0038 0.0045 −82% Metabolite - 1457 61 0.0038 0.0045 115% Metabolite - 3364 61 0.0039 0.0046 232% glyceric acid 50 0.0039 0.0046  78% Metabolite - 4615 61 0.004 0.0046 154% uridine-5-diphosphoglucose 50 0.004 0.0046 −35% Metabolite - 2323 61 0.0041 0.0047 111% Metabolite - 1333 61 0.0042 0.0047 −71% Metabolite - 3966 61 0.0042 0.0047 171% 3-methylglutaric acid 61 0.0043 0.0047 319% mercaptopyruvate 61 0.0043 0.0047 130% D-allose 50 0.0043 0.0047 −87% Metabolite - 1342-possible- 61 0.0043 0.0047 286% phenylacetylglutamine isoleucine 50 0.0044 0.0047  33% Metabolite - 3044 61 0.0046 0.0049 120% 4-acetamidobutyric acid 61 0.0048 0.0051 754% 3-phospho-l-serine 61 0.0051 0.0053 −22% Metabolite - 3531 61 0.0051 0.0053 160% Metabolite - 3514-retired-topiramate 61 0.0053 0.0055 170% inositol-1-phosphate 50 0.0054 0.0055  88% Metabolite - 1392 61 0.0057 0.0059 164% Metabolite - 2973 50 0.006 0.006 −38% Metabolite - 5214 50 0.0062 0.0062 −71% Metabolite - 1394-possible-Losartan 61 0.0063 0.0063 109% Metabolite - 4550 61 0.0065 0.0064  95% Metabolite - 3816 61 0.0065 0.0064 −92% Metabolite - 2141 61 0.0068 0.0066 181% Metabolite - 3526 61 0.0075 0.0072 196% Metabolite - 1831-possible-Cl-adduct-of- 61 0.0078 0.0075  86% citrulline Metabolite - 1330 61 0.0079 0.0076 216% spermine 50 0.0085 0.0081 −91% Metabolite - 5186 61 0.0087 0.0081 879% Metabolite - 4637 50 0.0087 0.0081 −28% Metabolite - 3708 61 0.0087 0.0081  61% Metabolite - 1496 61 0.0087 0.0081  46% 3-methyl-L-histidine 61 0.009 0.0082  51% Metabolite - 3668 61 0.009 0.0082 132% taurine 61 0.0091 0.0083 −42% Metabolite - 5210 50 0.0092 0.0083 −44% histidine 50 0.0093 0.0083  39% Metabolite - 3522 61 0.0094 0.0084 182% Metabolite - 4014 50 0.01 0.0088 104% quinolinic acid 61 0.0101 0.0088  93% Metabolite - 1465 61 0.0101 0.0088 124% Metabolite - 3539 61 0.0102 0.0089 460% Metabolite - 1186 61 0.0105 0.0091 −82% arachidonic acid 50 0.0106 0.0091  74% Metabolite - 3998 50 0.0108 0.0092  32% carnitine 61 0.0111 0.0094  74% Metabolite - 2900- 61 0.0111 0.0094 103% alanine 50 0.0115 0.0096  54% Isobar-6-includes-valine-betaine 61 0.0115 0.0096  30% Metabolite - 3997 61 0.0116 0.0096 740% Metabolite - 3554 61 0.0121 0.0099 226% Metabolite - 2111 61 0.0121 0.0099  76% Metabolite - 4019 50 0.0125 0.0102 −35% Metabolite - 3221 61 0.0128 0.0103 107% Metabolite - 5110 61 0.0132 0.0107 131% N-acetyl-D-glucosamine 50 0.0134 0.0107 −42% 4-Guanidinobutanoic acid 61 0.0136 0.0108  98% 5-s-methyl-5-thioadenosine 61 0.0137 0.0108 152% Metabolite - 5087 61 0.014 0.011 105% ribulose-5-phosphate 50 0.014 0.011 −51% Metabolite - 3020 50 0.0146 0.0114  76% Metabolite - 3436 61 0.0152 0.0118 213% 3-amino-isobutyrate 50 0.0162 0.0125 2172%  Metabolite - 3955 61 0.0171 0.0131 −25% Metabolite - 2897 61 0.0171 0.0131 121% Metabolite - 3220 61 0.0175 0.0133 235% Metabolite - 5089 61 0.0177 0.0134 133% Metabolite - 2690 61 0.0191 0.0142 784% suberic acid 61 0.0191 0.0142  79% Metabolite - 2688 61 0.0191 0.0142 −18% Metabolite - 4235 61 0.0193 0.0142 140% Metabolite - 1974 61 0.0194 0.0142 289% gamma-L-glutamyl-L-glutamine 61 0.0195 0.0142 −68% Metabolite - 2347 61 0.02 0.0145  69% Metabolite - 4706 61 0.0201 0.0145 184% picolinic acid 61 0.0201 0.0145 131% Metabolite - 2143 61 0.0204 0.0146 408% Metabolite - 4866 61 0.0209 0.0149 −79% Metabolite - 4018 61 0.0212 0.015 564% 2-acetamido-1-amino-1-2-dideoxy-beta-D- 50 0.0214 0.0151 −46% glucopyranose adenosine 61 0.022 0.0155 −56% sarcosine 50 0.0225 0.0157 795% Metabolite - 1069-possible- 61 0.0229 0.0159 −79% dehydroepiandrosterone-sulfate- DL-cystathionine 50 0.0233 0.0161 203% Metabolite - 5086 61 0.0236 0.0163  53% Metabolite - 3064 61 0.0242 0.0166  96% L-kynurenine 50 0.0252 0.0172 347% pyrophosphate 50 0.0256 0.0173  84% Spermidine 50 0.0257 0.0173 −92% Metabolite - 1961-retired-glycocholic acid 61 0.0257 0.0173 444% Metabolite - 1608 61 0.0261 0.0174 −60% Metabolite - 5109 61 0.0261 0.0174 156% 3-hydroxybutanoic acid 50 0.0263 0.0174 305% Metabolite - 1114 61 0.0264 0.0174  33% Metabolite - 5232 50 0.0267 0.0176 150% N-acetylserotonin 50 0.0272 0.0178 192% Metabolite - 3984 61 0.0287 0.0187 468% Metabolite - 1595-possible-glutathione- 61 0.0293 0.019 −66% metabolite trans-4-hydroxyproline 50 0.0308 0.0199  89% Metabolite - 4593 50 0.0311 0.02  56% Carnosine 61 0.0326 0.0209  63% Metabolite - 2139 61 0.0342 0.0218  88% beta-alanine 50 0.0345 0.0218  74% Metabolite - 2390 61 0.0345 0.0218 343% Metabolite - 2292 61 0.035 0.022 −76% Metabolite - 2075 61 0.0355 0.0222 −75% Metabolite - 4331 61 0.0357 0.0222  56% Metabolite - 2108 61 0.0374 0.0232  44% Metabolite - 3370 61 0.0394 0.0242  31% Metabolite - 4168 61 0.0394 0.0242  49% Metabolite - 4868-possible-Bradykinin 61 0.0397 0.0243 −61% DL-homocysteine 61 0.0409 0.0249  45% sn-Glycerol-3-phosphate 50 0.0414 0.0251 322% Metabolite - 3139 61 0.0421 0.0254 −31% Metabolite - 5126 61 0.0422 0.0254 −38% Metabolite - 1129 61 0.0427 0.0256 −56% Metabolite - 1203-possible-acetylbrowniine- 61 0.0434 0.0258 −62% tricornine-germine-or-veracevine Metabolite - 5108 61 0.0435 0.0258  95% Metabolite - 4027 50 0.0437 0.0259 153% Metabolite - 2099 61 0.0442 0.0261 −68% arginino-succinate 61 0.0455 0.0267 −50% L-allo-threonine 50 0.0477 0.0279  26% Metabolite - 2853 61 0.0497 0.029 124% Metabolite - 1576 61 0.0509 0.0295  41% Metabolite - 1303 61 0.0513 0.0296 −60% noradrenaline 50 0.0515 0.0296  26% Metabolite - 1713 61 0.0518 0.0297  71% Metabolite - 2778 61 0.0537 0.0305 269% sorbitol 50 0.0539 0.0305 1085%  Metabolite - 1718 61 0.0539 0.0305 −51% Metabolite - 4567 61 0.0542 0.0305 1058%  gamma-glu-cys 61 0.0543 0.0305 −73% caffeine 61 0.0545 0.0305 −71% Metabolite - 5167 61 0.0553 0.0308  72% fumaric acid 50 0.0577 0.032  63% Metabolite - 3379 61 0.0578 0.032 −26% Metabolite - 1970 61 0.059 0.0324 102% Metabolite - 2406 61 0.0591 0.0324 159% threonine 50 0.0604 0.033  24% Metabolite - 2072 61 0.0621 0.0338 417% Metabolite - 5229 50 0.0624 0.0339 −32% Metabolite - 3960 61 0.0638 0.0343 −30% Metabolite - 3022 50 0.0639 0.0343  79% Metabolite - 5166 61 0.064 0.0343  72% Metabolite - 3056 61 0.0644 0.0344 1785%  Metabolite - 2027 61 0.0656 0.0349 301% Metabolite - 4015 50 0.0678 0.0359  72% Metabolite - 2118 61 0.0694 0.0366 −26% Metabolite - 1070 61 0.0695 0.0366 −41% Metabolite - 4516 50 0.0724 0.038 −50% Metabolite - 4365 50 0.0728 0.038 −56% Metabolite - 2806 61 0.0732 0.038 −26% Metabolite - 3896 61 0.0733 0.038 179% glutathione-reduced 61 0.0737 0.0381 −78% Metabolite - 3180 61 0.0751 0.0387  88% 3-methyl-2-oxovaleric acid 61 0.0755 0.0388 170% Metabolite - 3073 50 0.0767 0.0392 138% Metabolite - 1327-possible-bilirubin 61 0.0774 0.0395  41% Metabolite - 3238 61 0.0778 0.0395 247% L-homoserine-lactone 61 0.0786 0.0398 −24% uridine-5-diphosphoglucuronic acid 50 0.0813 0.041 114% Metabolite - 1248-possible-avermectin- 61 0.0825 0.0414 −39% aglycone Metabolite - 3974 61 0.0853 0.0427  55% Isobar-27-includes-L-kynurenine-alpha-2- 61 0.0883 0.0441 293% diamino-gamma-oxobenzenebutanoic acid tyrosine 61 0.0893 0.0444  19% Metabolite - 2105 61 0.0921 0.0456 −42% Metabolite - 4017 50 0.0939 0.0462 −24% saccharopine 61 0.094 0.0462  47% Metabolite - 3002 50 0.0977 0.0479  25% Metabolite - 4512 50 0.0991 0.0484 −55% Metabolite - 1977 61 0.1008 0.0491  51% Metabolite - 3771 61 0.1044 0.0506 −25% Metabolite - 3498 61 0.1048 0.0506 −28% aspartate 61 0.1051 0.0506 −34% Metabolite - 1142-possible-5- 61 0.1082 0.0519 407% hydroxypentanoate-or-beta- hydroxyisovaleric acid Metabolite - 4058 50 0.1102 0.0528 −38% Metabolite - 3980 61 0.1154 0.0551 −39% Metabolite - 1111-possible- 61 0.1163 0.0552 −22% methylnitronitrosoguanidine-or-ethyl- thiocarbamoylacetate 5-hydroxyindoleacetate 50 0.1165 0.0552 −90% Metabolite - 2121 61 0.1176 0.0556  84% Metabolite - 3183-possible-gamma-L- 61 0.1218 0.0574  64% glutamyl-L-phenylalanine-or-aspartame Metabolite - 3951 61 0.1225 0.0575  45% Metabolite - 3832-possible-phenol-sulfate 61 0.1246 0.0583 327% Metabolite - 2703 61 0.1261 0.0588 −26% Metabolite - 3756 61 0.1282 0.0596 454% Metabolite - 2546 61 0.1302 0.0603 197% Metabolite - 3016 50 0.1372 0.0633 −39% Metabolite - 3810 61 0.1419 0.0653 −31% Metabolite - 3534 61 0.1426 0.0654  67% melatonin 50 0.1438 0.0657  55% Metabolite - 2821 61 0.1453 0.0662 300% Metabolite - 1497 61 0.1469 0.0668 −25% alpha-L-sorbopyranose 50 0.1505 0.0682 −26% Metabolite - 3696-retired-isobar- 61 0.152 0.0686 230% glycochenodeoxycholic acid- glycodeoxycholic acid Metabolite - 2724 61 0.1527 0.0687  52% Metabolite - 4787 61 0.1533 0.0688 −84% Metabolite - 4117-possible-propranolol-or-2- 61 0.1541 0.0689  39% heptyl-3-hydroxy-quinolone Metabolite - 1573 61 0.1548 0.069  56% beta-nicotinamide-adenine-dinucleotide 61 0.1581 0.0702 352% alpha-D-ribose-5-phosphate 50 0.1585 0.0702 −29% Metabolite - 1593 61 0.16 0.0707 −56% glycine 50 0.1614 0.0711  20% glutarate 61 0.1633 0.0717  73% succinate 50 0.1697 0.074  84% Metabolite - 1113-possible-acetylcarnitine- 61 0.1697 0.074  30% or-isopentyl-adenine Metabolite - 3034 50 0.1726 0.0751  38% Metabolite - 3848 61 0.1771 0.0767 −29% Metabolite - 5228 50 0.1777 0.0767  22% Metabolite - 3099 50 0.1779 0.0767 −25% GABA 50 0.1788 0.0768 −35% serine 50 0.1792 0.0768 −13% Metabolite - 4133 50 0.1803 0.077  64% Metabolite - 2036 61 0.1835 0.078 246% Metabolite - 2824 61 0.1838 0.078  98% possible-ISOBAR-DL-aspartic acid- 50 0.1848 0.0782 −29% maltose 50 0.1852 0.0782 504% Metabolite - 4503 50 0.1887 0.0794 335% Metabolite - 2827 61 0.1937 0.0813 −56% Metabolite - 3129 61 0.1989 0.0832 −13% Metabolite - 1616 61 0.1999 0.0834 −64% Metabolite - 3604 61 0.2032 0.0845 −40% Metabolite - 5128 61 0.2076 0.0861 −72% Isobar-5-includes-asparagine-ornithine 61 0.2092 0.0865  30% 1-7-dihydro-6h-purin-6-one 61 0.2113 0.0867  14% 1-methyladenine 50 0.2113 0.0867 −83% Metabolite - 4030-possible-glutethimide-or- 61 0.2115 0.0867 −28% securinine ornithine 50 0.2134 0.0872 −20% Metabolite - 1679 61 0.2163 0.0881  56% methionine 61 0.218 0.0886  17% biotin 61 0.2198 0.0891 −35% Metabolite - 4116 61 0.2206 0.0892 −13% Metabolite - 1963 61 0.229 0.0923 −27% Metabolite - 1575 61 0.2338 0.0938 −37% S-adenosyl-l-homocysteine 61 0.234 0.0938  22% dethiobiotin 50 0.2429 0.0971 −11% Metabolite - 1216 61 0.2446 0.0975  36% Metabolite - 1455 61 0.2458 0.0977 206% Metabolite - 4080 50 0.2467 0.0977 −39% biliverdin 61 0.2525 0.0998 −25% Metabolite - 5226 50 0.2569 0.1012  50% 25-hydroxycholesterol 50 0.258 0.1014  −6% Metabolite - 2129 61 0.2623 0.1028 −44% N-acetylneuraminate 61 0.265 0.1036  29% Metabolite - 2109 61 0.2658 0.1036  27% Metabolite - 3517 61 0.2721 0.1057  67% Metabolite - 4667 61 0.2746 0.1064 −22% cytidine-5-monophosphate 61 0.2754 0.1064  27% Metabolite - 4003 61 0.276 0.1064 −19% Metabolite - 3074 50 0.2779 0.1069  80% Metabolite - 2774 61 0.2815 0.1079  34% Metabolite - 5170 61 0.2827 0.1081 −98% Metabolite - 2768 61 0.285 0.1087 −99% dihydroxyacetone-phosphate 61 0.295 0.1122 −22% glucarate 50 0.3014 0.1144 −73% Metabolite - 2055 61 0.303 0.1145  27% Metabolite - 2368 61 0.3043 0.1145 −87% pyridoxamine-phosphate 61 0.3047 0.1145 −24% phosphoenolpyruvate 50 0.3051 0.1145 −43% fructose 50 0.3065 0.1148 −44% Metabolite - 5227 50 0.3077 0.1149 740% Metabolite - 1088 61 0.3104 0.1155 −36% 2-deoxy-D-ribose 61 0.311 0.1155  17% phytonadione 50 0.3221 0.1194 −11% Metabolite - 1609 61 0.3244 0.1199 −33% malic acid 50 0.3288 0.1212  17% Metabolite - 4796 50 0.3315 0.1218  26% 6-phosphogluconic acid 61 0.3322 0.1218  16% benzoic acid 50 0.333 0.1218  13% Metabolite - 1211 61 0.3356 0.1224  10% Metabolite - 4806 50 0.3385 0.1232  18% 2-deoxyuridine 61 0.3422 0.1237  31% cholesterol 50 0.3424 0.1237  −9% Metabolite - 3545 61 0.3426 0.1237  51% Isobar-1-includes-mannose-fructose- 61 0.3458 0.1246 −17% glucose-galactose-alpha-L-sorbopyranose- Inositol-D-allose Metabolite - 2056 61 0.3473 0.1248  18% 3-hydroxy-3-methylglutarate 50 0.3496 0.1253  22% 4-hydroxy-2-quinolinecarboxylic acid 61 0.3526 0.1261 −14% Metabolite - 1653 61 0.3592 0.1277 −25% xylitol 50 0.3596 0.1277  35% Metabolite - 2348 61 0.3597 0.1277  48% Metabolite - 3401 61 0.3637 0.1287  41% Isobar-40-includes-Maltotetraose-stachyose 61 0.3704 0.1308 −36% hippuric acid 61 0.3719 0.131 −29% glutamic acid 50 0.3743 0.1315  17% Metabolite - 2232 61 0.3753 0.1315 −36% Metabolite - 4362 50 0.381 0.1332 −21% Isobar-4-includes-Gluconic acid-DL- 61 0.3854 0.1344 −23% arabinose-D-ribose-L-xylose-DL-lyxose-D- xylulose Metabolite - 3430 61 0.3874 0.1348  29% Metabolite - 2313 61 0.3923 0.1361  14% 5-6-dihydrouracil 61 0.3941 0.1361  31% Metabolite - 1104 61 0.3943 0.1361 −23% Metabolite - 4355 50 0.3949 0.1361 −10% Metabolite - 2074 61 0.4055 0.1394  45% Metabolite - 2981 50 0.4071 0.1396  8% Metabolite - 3094 50 0.4099 0.1402  −8% Metabolite - 5189 61 0.4199 0.1433 −42% Metabolite - 2194 61 0.4272 0.1454  43% Metabolite - 2558 61 0.4305 0.146  35% Metabolite - 4514 50 0.4309 0.146  17% alpha-keto-glutarate 61 0.4325 0.1461  56% Metabolite - 5211 50 0.4333 0.1461 −47% cytidine 61 0.4411 0.1482  39% Isobar-30-includes-maltotetraose-stachyose 61 0.4415 0.1482 −24% Metabolite - 2198 61 0.4438 0.1486 −14% Metabolite - 2389 61 0.447 0.1493 −10% histamine 61 0.4668 0.1556 −12% Metabolite - 3952 61 0.4773 0.1584 −19% ascorbic acid 50 0.4775 0.1584  31% Metabolite - 3694 61 0.4868 0.1611 −28% Isobar-17-includes-arginine-N-alpha-acetyl- 61 0.4925 0.1625 −15% ornithine Metabolite - 1187 61 0.4931 0.1625 −24% Metabolite - 4931 61 0.4969 0.1633  16% Metabolite - 2319 61 0.5011 0.1643 −27% Metabolite - 3189 61 0.5094 0.1667  39% Metabolite - 3752 61 0.5167 0.1687  43% Metabolite - 5215 50 0.5182 0.1688 −10% Metabolite - 3484 61 0.5253 0.1707  32% Metabolite - 3365 61 0.5283 0.171 −22% Isobar-21-includes-gamma-aminobutyryl-L- 61 0.5285 0.171 −20% histidine-L-anserine Metabolite - 4272 50 0.5403 0.1742 −13% Metabolite - 2185 61 0.5423 0.1742  19% Metabolite - 3755 61 0.5431 0.1742  10% Metabolite - 4497 50 0.5432 0.1742 −14% orotidine-5-phosphate 61 0.5557 0.1777 −20% Metabolite - 3051 61 0.5569 0.1777  26% N-5-aminocarbonyl-L-ornithine 50 0.5705 0.1813  18% Metabolite - 3317 61 0.5709 0.1813  19% Metabolite - 2041 61 0.5719 0.1813  16% Metabolite - 2846 61 0.5851 0.1844 −25% cystine 50 0.5862 0.1844 −11% Metabolite - 4032 50 0.5865 0.1844  26% azelaic acid 61 0.5881 0.1844  16% Metabolite - 3475 61 0.5881 0.1844 −17% o-phosphoethanolamine 50 0.5905 0.1847  18% cysteine 50 0.5935 0.1853  21% Metabolite - 1911 61 0.5982 0.1861  22% Metabolite - 4053 50 0.5988 0.1861  15% galactose 50 0.6145 0.1906 −10% Metabolite - 1183 61 0.6174 0.191 −22% Metabolite - 2212 61 0.6301 0.1946  31% hypotaurine 50 0.6325 0.1949  18% Metabolite - 3957 61 0.6423 0.1975  11% Metabolite - 1286 61 0.646 0.1982  −6% Isobar-22-includes-glutamic acid-O-acetyl-L- 61 0.6499 0.199  6% serine Metabolite - 4616 61 0.6537 0.1997 −16% Isobar-32-includes-N-acetyl-D-glucosamine- 61 0.659 0.2009  −8% N-acetyl-D-mannosamine Metabolite - 2407 61 0.6631 0.2017  −8% N-N-dimethylarginine 61 0.6691 0.2031  13% Metabolite - 4354 50 0.6766 0.2049  −8% Metabolite - 4510 50 0.6881 0.2076  11% tyramine 50 0.6893 0.2076  5% Metabolite - 4632 50 0.6898 0.2076  6% Metabolite - 4271 50 0.6928 0.2081  22% Metabolite - 3994 61 0.6964 0.2087 −16% Metabolite - 1085-possible-isolobinine-or-4- 61 0.6977 0.2087  −8% aminoestra-1-3-5-10-triene-3-17beta-diol Metabolite - 4448 61 0.7093 0.2117  −6% Metabolite - 2064 61 0.7138 0.2126  7% glutamine 50 0.7211 0.2143  12% guanine 50 0.7322 0.2172 −14% Metabolite - 2279 61 0.7424 0.2195 −10% 4-methyl-2-oxopentanoate 61 0.7432 0.2195  12% Metabolite - 3040 50 0.7523 0.2217  −7% Metabolite - 2691 61 0.7568 0.2226  11% Isobar-18-includes-D-fructose-1-phosphate- 61 0.7623 0.2233  −9% beta-D-fructose-6-phosphate Metabolite - 4523 50 0.7627 0.2233  6% Metabolite - 3027 50 0.7639 0.2233  10% Metabolite - 4043 50 0.7735 0.2254  4% dulcitol 50 0.7743 0.2254  −6% beta-D-lactose 50 0.7821 0.2272  11% Metabolite - 1975 61 0.7859 0.2279  11% 5-oxoproline 50 0.7882 0.2281  5% Metabolite - 3813 61 0.7998 0.2306  14% 3-nitro-L-tyrosine 50 0.8003 0.2306  8% Metabolite - 4002 50 0.815 0.2344  5% Metabolite - 4238 61 0.8303 0.2383  8% Metabolite - 2388 61 0.8374 0.2399  −4% Metabolite - 3783 61 0.8416 0.2406  −6% Metabolite - 4084 50 0.849 0.2422  1% Metabolite - 3778 61 0.8508 0.2423  −8% Metabolite - 3476 61 0.8527 0.2423  −6% Metabolite - 1979-Cl-adduct-of-C6H10O5 61 0.8564 0.2426  −5% Metabolite - 1980 61 0.8571 0.2426  9% Metabolite - 2005 61 0.8602 0.243  5% alpha-amino-adipate 50 0.862 0.243  7% Metabolite - 5213 50 0.8674 0.2441  −4% glucose-6-phosphate 50 0.8781 0.2461  −4% Metabolite - 2174 61 0.8781 0.2461  5% Metabolite - 5147 61 0.881 0.2464 −14% Metabolite - 4020 50 0.8947 0.2493  3% adenine 50 0.8948 0.2493  −4% Metabolite - 3176-possible-creatine 61 0.9158 0.2547  −1% Metabolite - 2180 61 0.937 0.26  3% Metabolite - 3576 61 0.9487 0.2624  2% 2-deoxyuridine-5-triphosphate 61 0.9491 0.2624  −2% L-histidinol 61 0.9545 0.2629  1% Metabolite - 4096-gamma-glu-gly-leu- 61 0.9545 0.2629  −1% Metabolite - 2753 61 0.9699 0.2664  1% Isobar-31-includes-maltotriose-melezitose 61 0.9712 0.2664  1% glucono-gamma-lactone 50 0.9791 0.2681  1% uridine-5-monophosphate 61 0.9814 0.2682  0% asparagine 50 0.9893 0.2695  0% mannose 50 0.9898 0.2695  0% 2-deoxy-D-glucose 50 0.9996 0.2716  0%

Example 2 Cancer Vs. Non-Cancer

Biomarkers were discovered by (1) analyzing plasma and/or urine samples from different groups of human subjects to determine the levels of metabolites in the samples and then (2) statistically analyzing the results to determine those metabolites that were differentially present in the two groups.

The plasma and/or urine samples used for the analysis were from 53 control individuals with negative biopsies for prostate cancer and 48 individuals with prostate cancer. After the levels of metabolites were determined, the data was analyzed using univariate T-tests (i.e., Welch's T-test).

T-tests were used to determine differences in the mean levels of metabolites between two populations (i.e., Prostate cancer vs. Control plasma, Prostate cancer vs. Control urine).

Biomarkers:

As listed below in Table 4, biomarkers were discovered that were differentially present between plasma samples from subjects with prostate cancer and Control subjects with negative prostate biopsies (i.e. not diagnosed with prostate cancer). Table 5 lists biomarkers that were discovered that were differentially present between urine samples from subjects with prostate cancer and Control subjects (i.e. not diagnosed with prostate cancer).

Tables 4 and 5 include, for each listed biomarker, the p-value and the q-value determined in the statistical analysis of the data concerning the biomarkers and an indication of the percentage difference in the lower grade prostate cancer mean level as compared to the control mean level (Table 4) and the metastatic/high grade prostate cancer mean level as compared to the control mean level (Table 5). The term “Isobar” as used in the tables indicates the compounds that could not be distinguished from each other on the analytical platform used in the analysis (i.e., the compounds in an isobar elute at nearly the same time and have similar (and sometimes exactly the same) quant ions, and thus cannot be distinguished). Library indicates the chemical library that was used to identify the compounds. The number 50 refers to the GC library and the number 35 refers to the LC library.

TABLE 4 Prostate Cancer Biomarkers from Plasma from subjects with Prostate Cancer compared to Plasma from Control subjects. % Change COMPOUND Library p-value q-value in PCA Metabolite - 3377 35 0 0.0043 192% Metabolite - 2329 35 1.00E−04 0.0144 73% Metabolite - 3305 35 1.00E−04 0.0138 144% palmitoleic acid 50 4.00E−04 0.0313 67% Metabolite - 3327 35 5.00E−04 0.0313 111% Metabolite - 1127 35 6.00E−04 0.0313 54% DL-indole-3-lactic acid 50; 35 7.00E−04 0.0325 33% Metabolite - 3322 35 8.00E−04 0.0325 84% Metabolite - 1185 35 0.0012 0.045 −41% elaidic acid 50 0.0021 0.0665 54% Metabolite - 3603 35 0.0022 0.0665 −29% lactate 50 0.0035 0.0882 17% Metabolite - 2141 35 0.0036 0.0882 126% Metabolite - 5349 50 0.0037 0.0882 −19% Metabolite - 2711 35 0.0046 0.1028 27% caffeine 35 0.0049 0.1041 104% N-acetyl-L-valine 35 0.0061 0.1135 −18% monosaccharide 50 0.0063 0.1135 −18% Metabolite - 2108 35 0.0069 0.1135 65% Metabolite - 3402 35 0.007 0.1135 59% Metabolite - 2407 35 0.0071 0.1135 −34% n-hexadecanoic acid 50 0.0078 0.1191 19% Metabolite - 3030 50 0.0082 0.1196 −18% Metabolite - 1988 35 0.01 0.1398 43% alpha-keto-glutarate 35 0.0104 0.1398 81% Metabolite - 1121 35 0.0123 0.1555 −24% Isobar-17-includes-arginine-N-alpha- 35 0.0125 0.1555 −27% acetyl-ornithine Metabolite - 1104 35 0.0155 0.1863 20% Metabolite - 1116 35 0.0183 0.2034 78% Metabolite - 1286 35 0.0188 0.2034 −15% Metabolite - 1713 35 0.0189 0.2034 54% Metabolite - 3088 50 0.0199 0.2034 −30% Metabolite - 3977 35 0.0207 0.2034 20% theobromine-theophylline 35 0.0212 0.2034 74% Metabolite - 1839 35 0.0223 0.2034 85% valine 50 0.0224 0.2034 16% tartaric acid 35 0.0225 0.2034 61% Metabolite - 3033 50 0.0244 0.2034 −13% Metabolite - 1085-possible-isolobinine-or- 35 0.0247 0.2034 −17% 4-aminoestra-1-3-5-10-triene-3-17beta- diol glycerol 50 0.0247 0.2034 16% 3-methylglutaric acid 35 0.0249 0.2034 22% Metabolite - 1831-possible-Cl-adduct-of- 35 0.0253 0.2034 160% citrulline Metabolite - 3303 35 0.0278 0.2179 19% Metabolite - 3900 35 0.0294 0.2256 16% octadecanoic acid 50 0.0337 0.2512 11% Metabolite - 3843 35 0.0346 0.2512 22% Metabolite - 2978 50 0.035 0.2512 −22% Metabolite - 2005 35 0.0359 0.252 35% aspartate 50 0.041 0.2818 34% 3-hydroxybutanoic acid 50 0.0434 0.2924 81% Metabolite - 3832-possible-phenol-sulfate 35 0.0475 0.314 97% phenylalanine 35 0.05 0.3241 7% Metabolite - 3040 50 0.0517 0.329 −21% alpha-tocopherol 50 0.0529 0.3301 91% Metabolite - 3002 50 0.0539 0.3305 27% creatinine 35 0.0563 0.3392 11% dethiobiotin 50; 35 0.0603 0.3565 30% linoleic acid 50 0.0622 0.3598 17% L-homoserine 50 0.063 0.3598 −16% Metabolite - 3309 35 0.066 0.3707 37% Metabolite - 4147 50 0.0676 0.3735 32% 3-chloro-L-tyrosine 50 0.0704 0.3747 23% isoleucine 50 0.072 0.3747 15% Metabolite - 1834 35 0.0729 0.3747 54% Metabolite - 3781-possible-Na-adduct-of- 35 0.073 0.3747 −9% Isobar-21 Metabolite - 2390 35 0.0735 0.3747 38% 3-amino-isobutyrate 50 0.0756 0.3747 −13% Metabolite - 3098 50 0.0761 0.3747 −24% Metabolite - 2389 35 0.0767 0.3747 −65% Metabolite - 3178-possible-NH3-adduct- 35 0.0803 0.3786 16% of-isobar-42 Metabolite - 4031-possible- 35 0.0804 0.3786 14% norlevorphenol-isobutylphendienamide- amprolium alanine 50 0.0821 0.3786 18% leucine 50 0.0833 0.3786 15% Metabolite - 1817 35 0.084 0.3786 −20% Metabolite - 3146 35 0.0842 0.3786 49% Metabolite - 3830 35 0.0863 0.3791 31% alpha-4-dihydroxybenzenepropanoic acid 50 0.0866 0.3791 36% Metabolite - 1829 35 0.0881 0.3809 −13% Metabolite - 3534 35 0.0918 0.3827 43% Metabolite - 4511 50 0.0925 0.3827 37% Metabolite - 2285 35 0.0932 0.3827 217% D-quinic acid 50 0.094 0.3827 72% Isobar-6-includes-valine-betaine 35 0.0949 0.3827 8% Metabolite - 3707 35 0.0965 0.3827 −52% Metabolite - 3837 35 0.0965 0.3827 30% Metabolite - 3813 35 0.0991 0.3855 38% Metabolite - 2130 35 0.1005 0.3855 −43% Metabolite - 3014 50 0.1016 0.3855 19% Metabolite - 1836 35 0.1036 0.3855 39% tryptophan 50; 35 0.1049 0.3855 12% Metabolite - 3772 35 0.1052 0.3855 16% Metabolite - 3138 35 0.1056 0.3855 31% 4-methyl-2-oxopentanoate 50 0.1063 0.3855 20% glycine 50 0.1082 0.386 19% Metabolite - 3314 35 0.1088 0.386 28% Metabolite - 2254 35 0.112 0.3897 70% Metabolite - 2974 50 0.1132 0.3897 −13% p-acetamidophenyl-beta-D-glucuronide 35 0.1133 0.3897 392% Metabolite - 3489 35 0.1178 0.4011 54% Metabolite - 4362 50 0.1232 0.4152 −19% carnosine 35 0.1257 0.4163 24% melatonin 50 0.1259 0.4163 −11% Metabolite - 3758 35 0.1279 0.4163 62% Metabolite - 1609 35 0.1285 0.4163 23% Metabolite - 2698 35 0.1297 0.4163 42% trans-4-hydroxyproline 35; 50 0.1352 0.4205 −22% Metabolite - 2391 35 0.1363 0.4205 15% Metabolite - 4769 50 0.1368 0.4205 −15% Metabolite - 3074 50 0.1368 0.4205 70% Metabolite - 3067 50 0.1372 0.4205 13% adenosine-5-monophosphate 35 0.142 0.4314 22% Metabolite - 2287 35 0.1436 0.4322 −78% Metabolite - 2388 35 0.1466 0.4372 −10% cholesterol 50 0.1481 0.4372 11% Metabolite - 1975 35 0.15 0.4372 −35% Metabolite - 3016 50 0.1504 0.4372 10% heptanedioic acid 35 0.1522 0.4378 −33% glyceric acid 50 0.1532 0.4378 −16% Metabolite - 2506 35 0.1556 0.4395 63% azelaic acid 35 0.1571 0.4395 12% Metabolite - 3143 35 0.1583 0.4395 21% Metabolite - 5419 50 0.159 0.4395 29% Metabolite - 2867 35 0.1608 0.4408 100% Metabolite - 2056 35 0.1623 0.4412 17% Isobar-13-includes-5-keto-D-gluconic 35 0.1646 0.4439 24% acid-2-keto-L-gulonic acid-D-glucuronic acid glutamic acid 50 0.1716 0.4557 22% Metabolite - 2924 50 0.1732 0.4557 20% Metabolite - 3073 50 0.1743 0.4557 −19% Isobar-5-includes-asparagine-ornithine 35 0.1744 0.4557 −14% benzoic acid 50; 35 0.176 0.4557 −19% tyramine 50 0.1771 0.4557 14% Metabolite - 2255 35 0.183 0.459 −58% glucose-6-phosphate 50 0.1835 0.459 6% Metabolite - 1977 35 0.1838 0.459 14% Metabolite - 2316 35 0.1841 0.459 −36% 2-keto-L-gulonic acid 50 0.1863 0.459 5% n-dodecanoate 50 0.187 0.459 14% Metabolite - 3474 35 0.189 0.459 −19% ornithine 50 0.1892 0.459 22% L-beta-imidazolelactic acid 50; 35 0.1952 0.4677 10% 5-oxoproline 50 0.1956 0.4677 10% carnitine 35 0.197 0.4678 −9% glutamine 50 0.1993 0.4698 16% Metabolite - 3216 35 0.2052 0.4785 21% Metabolite - 2212 35 0.2067 0.4785 −17% fructose 50 0.2072 0.4785 29% Metabolite - 3091 50 0.2097 0.481 −32% Metabolite - 3109 50 0.216 0.4901 −25% Metabolite - 2507 35 0.2166 0.4901 54% histidine 50 0.2197 0.4937 13% Metabolite - 5403 50 0.2235 0.4991 −11% Metabolite - 1113-possible- 35 0.2254 0.5 14% acetylcarnitine-or-isopentyl-adenine catechol 35 0.2295 0.5058 53% Metabolite - 3019 50 0.232 0.508 −10% Metabolite - 4032 50 0.2399 0.5217 23% Metabolite - 2898 35 0.2417 0.5223 57% Metabolite - 3017 50 0.2449 0.5245 −13% 5-6-Dimethylbenzimidazole 50 0.2458 0.5245 25% Metabolite - 1211 35 0.2482 0.5261 −67% Metabolite - 2111 35 0.2497 0.5261 25% Metabolite - 3160 35 0.2544 0.5298 18% Metabolite - 4767 50 0.2546 0.5298 −19% threonine 50 0.2588 0.5353 9% Metabolite - 4042 50 0.2647 0.5433 8% Metabolite - 2269- 35 0.2698 0.5433 −25% Metabolite - 4078 35 0.2704 0.5433 23% Metabolite - 3215 35 0.2706 0.5433 15% Metabolite - 3624 35 0.2708 0.5433 23% Metabolite - 3085 50 0.2758 0.5438 13% Metabolite - 2914 50 0.2774 0.5438 −2% Metabolite - 1110 35 0.2792 0.5438 −31% Metabolite - 4167 35 0.2792 0.5438 20% Metabolite - 3752 35 0.282 0.5438 −60% Metabolite - 3877 35 0.2832 0.5438 28% Metabolite - 3165 35 0.2842 0.5438 7% glucono-gamma-lactone 50 0.2848 0.5438 7% Metabolite - 3578 35 0.2855 0.5438 −24% Metabolite - 3102 50 0.2897 0.5449 9% Metabolite - 3131 35 0.2901 0.5449 −23% Metabolite - 2027 35 0.2909 0.5449 18% Metabolite - 3972 35 0.2949 0.5488 −13% Metabolite - 1188 35 0.3012 0.5488 −17% Metabolite - 2279 35 0.3017 0.5488 36% arachidonic acid 50 0.304 0.5488 16% Metabolite - 3089 50 0.3056 0.5488 23% DL-cystathionine 35 0.3083 0.5488 −11% trans-2-3-4-trimethoxycinnamic acid 35 0.3128 0.5488 −26% p-hydroxybenzaldehyde 35 0.3129 0.5488 10% Metabolite - 3576 35 0.3151 0.5488 −17% Metabolite - 5489 50 0.3161 0.5488 −9% Metabolite - 4795 50 0.3168 0.5488 −18% Metabolite - 4504 50 0.3207 0.5488 16% Metabolite - 3025 50 0.321 0.5488 −9% fumaric acid 50 0.3213 0.5488 12% Isobar-36-includes-D-sorbitol-6- 35 0.3219 0.5488 16% phosphate-mannitol-1-phosphate Metabolite - 1203-possible- 35 0.3221 0.5488 33% acetylbrowniine-tricornine-germine-or- veracevine Metabolite - 3023 50 0.3225 0.5488 −9% xylitol 35; 50 0.3244 0.5488 −16% Metabolite - 2592 35 0.3264 0.5488 71% methyl-indole-3-acetate 35 0.3269 0.5488 14% Metabolite - 3313 35 0.3272 0.5488 61% Metabolite - 1498 35 0.3289 0.549 −13% Metabolite - 3184 35 0.3345 0.5509 15% serine 50 0.3365 0.5509 7% succinate 50 0.3397 0.5509 −7% GABA 50 0.3397 0.5509 12% Metabolite - 3027 50 0.3399 0.5509 −8% Metabolite - 1656 35 0.341 0.5509 −14% Metabolite - 3086 50 0.3429 0.5509 −19% malic acid 35 0.3478 0.5509 26% Metabolite - 4196 50 0.3484 0.5509 26% Metabolite - 5906 50 0.3494 0.5509 35% allantoin 35 0.351 0.5509 9% L-alpha-glycerophosphorylcholine 35 0.3514 0.5509 22% L-allo-threonine 50 0.3516 0.5509 7% Metabolite - 2347 35 0.3533 0.5509 −23% 3-methyl-L-histidine 35 0.3568 0.5509 6% Metabolite - 1914 35 0.3578 0.5509 −25% 3-phospho-d-glycerate 35 0.3582 0.5509 16% Isobar-20-includes-fumaric acid-3-methyl- 35 0.3628 0.5509 −17% 2-oxobutanoate urea 50 0.3643 0.5509 −6% 4-hydroxyphenylacetate 35; 50 0.3644 0.5509 5% Metabolite - 4611 50 0.3655 0.5509 7% Metabolite - 3807 35 0.366 0.5509 5% pyridoxal-phosphate 35 0.3699 0.5528 −4% Metabolite - 4361 50 0.3706 0.5528 −15% adonitol 50 0.3737 0.5551 5% N-N-dimethylarginine 35 0.3769 0.5553 9% pantothenic acid 35 0.3772 0.5553 20% Metabolite - 3012 50 0.384 0.5609 −7% DL-pipecolic acid 35 0.3851 0.5609 11% Isobar-4-includes-Gluconic acid-DL- 35 0.3861 0.5609 −12% arabinose-D-ribose-L-xylose-DL-lyxose- D-xylulose Metabolite - 4355 50 0.389 0.5609 24% DL-homocysteine 35 0.3893 0.5609 13% 25-hydroxycholesterol 50 0.3953 0.5642 4% Metabolite - 4272 50 0.3955 0.5642 13% Metabolite - 3130 35 0.3968 0.5642 18% diaminopimelic acid 50; 35 0.3989 0.5642 −8% Metabolite - 1835 35 0.4018 0.5642 −11% Metabolite - 2281 35 0.403 0.5642 39% Metabolite - 3498 35 0.4042 0.5642 −9% Metabolite - 4084 50 0.405 0.5642 22% proline 35; 50 0.4097 0.5684 8% Metabolite - 3058 50 0.4147 0.573 −13% Metabolite - 2888-possible-sulfated- 35 0.4195 0.5751 −11% Rosiglitazone Metabolite - 3078 50 0.4196 0.5751 −8% Metabolite - 1911 35 0.4221 0.5761 −20% inositol 50 0.4245 0.577 5% citrulline 50 0.428 0.5795 −6% Metabolite - 3055-possible-NH3-adduct- 35 0.4333 0.5828 −19% of-hippuric acid Metabolite - 4134 50 0.4339 0.5828 −6% Metabolite - 2546 35 0.4358 0.583 15% mannose 50 0.4399 0.5862 4% Metabolite - 4516 50 0.4457 0.5899 −6% Metabolite - 5437 50 0.4462 0.5899 27% Metabolite - 3955 35 0.4482 0.5902 −23% phosphoenolpyruvate 35 0.453 0.5922 10% Metabolite - 4234 35 0.4546 0.5922 9% Metabolite - 5847 50 0.455 0.5922 20% citric acid 50 0.4573 0.5922 4% Metabolite - 2915 50 0.4585 0.5922 −6% Metabolite - 3125 35 0.4693 0.6021 7% Metabolite - 3081 50 0.4697 0.6021 9% Metabolite - 3370 35 0.4719 0.6026 9% Metabolite - 4096-possible-gamma-glu- 35 0.4808 0.6117 −16% gly-leu- 4-amino-5-methyl-2-1H-pyrimidinone 35 0.4852 0.6129 9% Metabolite - 5427 50 0.4874 0.6129 4% Metabolite - 4163 35 0.4882 0.6129 13% Metabolite - 1216 35 0.489 0.6129 8% nonanate 50 0.4925 0.6137 −3% oxitryptan 35 0.4933 0.6137 10% Metabolite - 2139 35 0.4964 0.6153 −8% methionine 35 0.499 0.6163 4% Metabolite - 3783 35 0.5036 0.6193 −11% galactose 50 0.5059 0.6193 −6% sn-Glycerol-3-phosphate 50 0.5069 0.6193 5% Metabolite - 1208 35 0.5108 0.6218 19% Metabolite - 2250 35 0.5155 0.6239 −15% dulcitol 50 0.5164 0.6239 −6% Metabolite - 4133 50 0.5194 0.6239 −7% Metabolite - 2849-related-to-citric acid- 35 0.52 0.6239 −6% dopamine 50 0.5264 0.6251 13% Metabolite - 2386 35 0.5265 0.6251 17% Metabolite - 4986 50 0.5287 0.6251 −14% Metabolite - 3044 35 0.5288 0.6251 9% Metabolite - 3094 50 0.5302 0.6251 7% Metabolite - 2387 35 0.5328 0.6256 49% gamma-L-glutamyl-L-tyrosine 35 0.5344 0.6256 −4% Isobar-2-includes-3-amino-isobutyrate-2- 35 0.5412 0.6289 18% amino-butyrate-4-aminobutanoic acid- dimethylglycine-choline- L-homoserine-lactone 35 0.5419 0.6289 −13% Isobar-19-includes-D-saccharic acid-2- 35 0.5463 0.6289 −10% deoxy-D-galactose-2-deoxy-D-glucose-L- fucose-L-rhamnose gamma-L-glutamyl-L-glutamine 35 0.5465 0.6289 −11% Metabolite - 2567 35 0.5492 0.6289 −6% Metabolite - 1086 35 0.5505 0.6289 11% Metabolite - 4275 50 0.5511 0.6289 −6% Metabolite - 3077 50 0.5522 0.6289 −6% gamma-glu-leu 35 0.5551 0.6291 5% Metabolite - 3320-possible-pimpinellin-or- 35 0.5561 0.6291 27% tetrahydroxybenzophenone Metabolite - 3992-possible-Cl-adduct-of- 35 0.56 0.6314 −4% Formate-dimer Metabolite - 1389-possible-glucuronide- 35 0.5643 0.6335 14% form-of-Metabolite - 1359 Metabolite - 3020 50 0.5697 0.6335 −6% guanosine-5-diphosphate 35 0.5698 0.6335 9% Metabolite - 3426 35 0.5712 0.6335 2% Metabolite - 2185 35 0.5746 0.6335 −5% orotidine-5-phosphate 35 0.5748 0.6335 12% Metabolite - 3951 35 0.575 0.6335 5% biliverdin 35 0.5834 0.6407 9% Metabolite - 2313 35 0.5904 0.6449 7% Metabolite - 3056 35 0.5918 0.6449 5% normetanephrine 50 0.593 0.6449 −6% Metabolite - 2100 35 0.6011 0.6511 −4% tyrosine 50 0.6026 0.6511 4% Metabolite - 1142-possible-5- 35 0.611 0.6582 12% hydroxypentanoate-or-beta- hydroxyisovaleric acid Metabolite - 1597 35 0.6293 0.6744 −3% Metabolite - 3441 35 0.6319 0.6744 6% glycochenodeoxycholic 35 0.6334 0.6744 14% acid/glycodeoxycholic acid Metabolite - 5346 50 0.6341 0.6744 5% Metabolite - 3765 35 0.6389 0.6774 12% Metabolite - 2249 35 0.6409 0.6774 7% Metabolite - 4593 50 0.6542 0.6824 −2% hippuric acid 35 0.6581 0.6824 10% Metabolite - 3022 50 0.6591 0.6824 −5% niacinamide 35 0.6593 0.6824 11% phytonadione 50 0.6599 0.6824 4% 5-methoxytryptamine 50 0.6645 0.6824 −6% Metabolite - 2486 35 0.6655 0.6824 9% N-6-trimethyl-l-lysine 35 0.6657 0.6824 6% Metabolite - 2753 35 0.6692 0.6824 5% Metabolite - 3670 35 0.6697 0.6824 4% Metabolite - 1465 35 0.67 0.6824 6% Metabolite - 3604 35 0.6701 0.6824 −11% Metabolite - 3075 50 0.6739 0.6824 −6% Metabolite - 3879 35 0.6755 0.6824 −20% Metabolite - 4148 50 0.6761 0.6824 −7% Isobar-21-includes-gamma-aminobutyryl- 35 0.6781 0.6824 −7% L-histidine-L-anserine N-acetyl-L-leucine 35 0.6834 0.6858 13% D-allose 50 0.6934 0.6906 −5% Metabolite - 3181 35 0.6944 0.6906 5% Metabolite - 3099 50 0.697 0.6906 8% Metabolite - 2688 35 0.6986 0.6906 −8% 3-nitro-L-tyrosine 50; 35 0.7005 0.6906 8% Metabolite - 3653-Possible-stachydrine- 35 0.7021 0.6906 −12% Metabolite - 2392 35 0.7026 0.6906 18% Metabolite - 3132 35 0.7104 0.695 3% Isobar-25-includes-L-gulono-1-4-lactone- 35 0.7112 0.695 8% glucono-gamma-lactone- Metabolite - 1323-possible-4-sulfobenzyl- 35 0.7154 0.6971 3% alcohol heneicosanoic acid-methyl-ester 50 0.7198 0.6975 −4% guanidineacetic acid 35 0.7202 0.6975 −5% Metabolite - 1215 35 0.7223 0.6975 −11% hypoxanthine 35 0.7241 0.6975 5% Metabolite - 3134 35 0.7323 0.7008 13% Isobar-30-includes-maltotetraose- 35 0.7345 0.7008 5% stachyose Metabolite - 2853 35 0.7345 0.7008 7% Metabolite - 1244 35 0.7359 0.7008 6% Metabolite - 1342-possible- 35 0.7405 0.7032 −7% phenylacetylglutamine-or-formyl-N-acetyl- 5-methoxykynurenamine Metabolite - 3135 35 0.7437 0.7043 −11% pyrophosphate 35; 50 0.7499 0.7082 9% ethyl-3-indoleacetate 50 0.7704 0.7186 3% Metabolite - 3476 35 0.7739 0.7186 7% Metabolite - 1183 35 0.778 0.7186 −8% Isobar-9-includes-sucrose-beta-D-lactose- 35 0.7787 0.7186 5% D-trehalose-D-cellobiose-D-Maltose- palatinose-melibiose-alpha-D-lactose Metabolite - 4503 50 0.7819 0.7186 9% L-kynurenine 35 0.7838 0.7186 3% Metabolite - 3093 50 0.7844 0.7186 6% Metabolite - 4365 50 0.7869 0.7186 5% Metabolite - 3698 35 0.7885 0.7186 6% oxalacetic acid 35 0.7896 0.7186 5% inosine 35 0.7915 0.7186 −9% Metabolite - 1573 35 0.7953 0.7186 5% uric acid 35 0.7956 0.7186 1% vitamin-B6 50 0.7962 0.7186 2% Metabolite - 4020 50 0.7967 0.7186 1% Metabolite - 1351 35 0.7988 0.7186 3% alphahydroxybenzeneacetic acid 35 0.8012 0.7186 −3% Metabolite - 1915 35 0.8026 0.7186 −13% Metabolite - 3994 35 0.8031 0.7186 7% Metabolite - 3218 35 0.8059 0.7186 4% phenyl-beta-glucopyranoside 50 0.8076 0.7186 −4% Metabolite - 1289 35 0.8079 0.7186 5% histamine 35 0.81 0.7186 −5% Metabolite - 2370 35 0.8174 0.7197 −4% Metabolite - 2053 35 0.818 0.7197 4% Metabolite - 5907 50 0.8229 0.7197 −2% hydroxyacetic acid 50 0.8232 0.7197 −4% maltose 50 0.8234 0.7197 −4% Metabolite - 3761 35 0.824 0.7197 5% tryptamine 50 0.8298 0.7229 −3% Metabolite - 2809 35 0.8365 0.7257 5% Metabolite - 4768 50 0.8383 0.7257 6% Metabolite - 2256 35 0.8395 0.7257 4% Metabolite - 2973 50 0.8416 0.7257 −1% Metabolite - 3245 35 0.8492 0.7286 4% Metabolite - 2129 35 0.8493 0.7286 5% Metabolite - 4274 50 0.8535 0.7303 2% Metabolite - 1974 35 0.8572 0.7316 −4% Metabolite - 1220 35 0.8619 0.7331 −5% pyridoxamine-phosphate 35 0.8654 0.7331 −2% thyroxine 35 0.8659 0.7331 −2% N-acetylserotonin 50 0.868 0.7331 1% Metabolite - 3968 35 0.8698 0.7331 4% Metabolite - 3108 50 0.8722 0.7333 −1% Metabolite - 2055 35 0.8779 0.7362 −2% D-alanyl-D-alanine 35 0.8819 0.7378 2% Metabolite - 4791 50 0.8875 0.7401 5% Metabolite - 3816 35 0.8926 0.7401 2% Metabolite - 3167 35 0.8948 0.7401 3% 4-Guanidinobutanoic acid 35 0.895 0.7401 2% glycocholic acid 35 0.8957 0.7401 5% Isobar-1-includes-mannose-fructose- 35 0.8978 0.7401 2% glucose-galactose-alpha-L- sorbopyranose-Inositol-D-allose 3-hydroxyphenylacetate 35 0.9038 0.7415 1% Metabolite - 3004 50 0.9114 0.7415 2% noradrenaline 50 0.9121 0.7415 2% Metabolite - 2074 35 0.9154 0.7415 −2% Metabolite - 2686 35 0.9155 0.7415 −1% Metabolite - 3436 35 0.916 0.7415 −2% Metabolite - 4091-possible-gamma- 35 0.9173 0.7415 1% glutamyl-glutamic acid 3-methoxy-L-tyrosine 50 0.9174 0.7415 0% 7-8-dihydrofolic acid 35 0.9197 0.7415 −4% Metabolite - 4238 35 0.9224 0.7415 −2% glucarate 50 0.9243 0.7415 2% phosphate 50 0.9259 0.7415 1% Metabolite - 1979-Cl-adduct-of-C6H10O5 35 0.9351 0.7426 −1% Metabolite - 1335 35 0.9375 0.7426 −1% Metabolite - 3166 35 0.9387 0.7426 2% xanthine 35 0.9416 0.7426 −2% 3-hydroxypropanoate 50 0.9424 0.7426 1% Metabolite - 4015 50 0.9455 0.7426 0% Metabolite - 3183-possible-gamma-L- 35 0.946 0.7426 −1% glutamyl-L-phenylalanine-or-aspartame Metabolite - 1111-possible- 35 0.9467 0.7426 −1% methylnitronitrosoguanidine-or-ethyl- thiocarbamoylacetate Metabolite - 1843 35 0.9471 0.7426 −1% Metabolite - 3615 35 0.9529 0.7437 −1% octopamine 50 0.9564 0.7447 1% Isobar-18-includes-D-fructose-1- 35 0.9652 0.7498 0% phosphate-beta-D-fructose-6-phosphate Metabolite - 3003 50 0.9736 0.7546 0% Metabolite - 5366 50 0.983 0.757 0% Metabolite - 4360 50 0.9831 0.757 −1% Metabolite - 3097 50 0.9839 0.757 −1% Metabolite - 1392 35 0.9857 0.757 1% Metabolite - 3129 35 0.9973 0.7602 0% Metabolite - 1133-possible-Na-adduct-of- 35 0.9981 0.7602 0% EDTA Metabolite - 3243 35 0.9986 0.7602 0% Metabolite - 1349 35 0.9989 0.7602 0%

TABLE 5 Prostate Cancer Biomarkers from Urine from subjects with Prostate Cancer compared to Urine from Control subjects. % Change COMPOUND Library p-value q-value in PCA Metabolite - 2974 50 0.0023 0.1555 −32% 2-amino-butyrate 50 0.0035 0.1555 −27% guanidineacetic acid 35 0.0048 0.1555 −65% citrulline 50 0.006 0.1555 −43% Metabolite - 2752 35 0.0063 0.1555 −38% adenosine 35 0.0067 0.1555 −46% Metabolite - 2242 35 0.0068 0.1555 170% 3-methoxy-4-hydroxyphenylacetate 50 0.0096 0.1555 −41% Metabolite - 4504 50 0.0118 0.1555 −36% N-acetyl-D-glucosamine 50 0.012 0.1555 −53% Metabolite - 2978 50 0.012 0.1555 −29% Metabolite - 1573 35 0.013 0.1555 −28% Metabolite - 2181 35 0.0134 0.1555 −34% Metabolite - 4522 50 0.0149 0.1555 −35% serine 50 0.016 0.1555 −38% methionine 35 0.0162 0.1555 −32% catechol 35 0.0163 0.1555 −60% Metabolite - 3215 35 0.0164 0.1555 −31% Metabolite - 4593 50 0.0167 0.1555 −36% Isobar-9-includes-sucrose-beta-D- 35 0.017 0.1555 −49% lactose-D-trehalose-D-cellobiose-D- Maltose-palatinose-melibiose-alpha-D- lactose Metabolite - 2567 35 0.0188 0.1611 −33% uracil 50 0.0194 0.1611 −36% Metabolite - 3020 50 0.0217 0.1611 −41% Metabolite - 3807 35 0.0225 0.1611 −25% arabinose 50 0.0227 0.1611 −47% histamine 35 0.0249 0.1611 −37% Metabolite - 3761 35 0.026 0.1611 −31% Metabolite - 3443 35 0.0262 0.1611 100% DL-homocysteine 35 0.0263 0.1611 −43% Metabolite - 2271 35 0.0264 0.1611 −47% Metabolite - 4503 50 0.0264 0.1611 −38% glycine 50 0.0284 0.1641 −37% Isobar-4-includes-Gluconic acid-DL- 35 0.0297 0.1641 −33% arabinose-D-ribose-L-xylose-DL-lyxose- D-xylulose adenosine-3-5-cyclic-monophosphate 35 0.0301 0.1641 −24% citric acid 50 0.0304 0.1641 −38% dopamine 50 0.0317 0.1641 −30% Metabolite - 1974 35 0.0347 0.1641 −42% N-acetylneuraminate 50 0.0348 0.1641 −32% Metabolite - 3381 35 0.0352 0.1641 −35% adenine 50 0.0359 0.1641 −37% Metabolite - 2051 35 0.0361 0.1641 −22% serotonin 35 0.0364 0.1641 −33% Metabolite - 4636 50 0.0396 0.1641 −44% Isobar-19-includes-D-saccharic acid-2- 35 0.0416 0.1641 −58% deoxy-D-galactose-2-deoxy-D-glucose- L-fucose-L-rhamnose L-allo-threonine 50 0.0421 0.1641 −31% Metabolite - 1349 35 0.0426 0.1641 −36% N-6-trimethyl-l-lysine 35 0.0433 0.1641 −38% Metabolite - 3370 35 0.0481 0.1641 −35% Metabolite - 3056 35 0.0489 0.1641 −29% Metabolite - 3803 35 0.049 0.1641 −41% 1-methyladenosine 35 0.0501 0.1641 −34% N-tigloylglycine 35 0.0501 0.1641 −31% tyrosine 50 0.0503 0.1641 −31% threonine 50 0.0516 0.1641 −31% Metabolite - 3951 35 0.0518 0.1641 −25% carnosine 35 0.0542 0.1641 −37% xylitol 35; 50 0.055 0.1641 −35% caffeine 35 0.0555 0.1641 79% Metabolite - 2277 35 0.0558 0.1641 −28% Metabolite - 1979-Cl-adduct-of- 35 0.0565 0.1641 −45% C6H10O5 heptanedioic acid 35 0.0573 0.1641 −28% orotic acid 50 0.0584 0.1641 −44% Metabolite - 1342-possible- 35 0.0596 0.1641 −29% phenylacetylglutamine-or-formyl-N- acetyl-5-methoxykynurenamine beta-hydroxyisovaleric acid 50 0.0598 0.1641 −34% Metabolite - 1911 35 0.0613 0.1641 −50% ornithine 50 0.0615 0.1641 −34% Metabolite - 4499 50 0.0619 0.1641 −36% Metabolite - 4519 50 0.0619 0.1641 −55% Metabolite - 2390 35 0.0635 0.1641 −43% Metabolite - 3329 35 0.064 0.1641 −59% 7-8-dihydrofolic acid 35 0.0648 0.1641 −26% Metabolite - 4251 50 0.0652 0.1641 −40% Metabolite - 1655 35 0.0659 0.1641 −51% Metabolite - 3955 35 0.0663 0.1641 −43% N-acetyl-L-valine 35 0.0669 0.1641 −51% 3-ureidopropionic acid 35 0.0672 0.1641 −49% erythrose-4-phosphate 50 0.0681 0.1641 −45% Metabolite - 3033 50 0.0691 0.1641 −35% gluconic acid 50 0.0697 0.1641 −46% succinate 50 0.0704 0.1641 −41% 2-acetamido-1-amino-1-2-dideoxy-beta- 50 0.0705 0.1641 −36% D-glucopyranose fructose 50 0.072 0.1641 −43% Metabolite - 3327 35 0.0729 0.1641 −46% 5-6-Dihydrothymine 35 0.0731 0.1641 −34% Metabolite - 3908 35 0.0744 0.1641 −39% Metabolite - 3305 35 0.0747 0.1641 −43% Metabolite - 4502 50 0.0748 0.1641 −5% phenylalanine 35 0.0753 0.1641 −25% Metabolite - 3055-possible-NH3-adduct- 35 0.0765 0.1641 46% of-hippuric acid pyrophosphate 35; 50 0.0783 0.1641 −53% Metabolite - 3183-possible-gamma-L- 35 0.079 0.1641 −37% glutamyl-L-phenylalanine-or-aspartame guanine 35 0.0803 0.1641 −30% Metabolite - 3970 35 0.0804 0.1641 −31% Metabolite - 3246-possible-Ala-GLy- 35 0.0816 0.1641 −38% glycyl-sarcosine-or-ureido-butyric acid 5-6-Dimethylbenzimidazole 50 0.0818 0.1641 −47% urocanic acid 35 0.0821 0.1641 −35% Metabolite - 3813 35 0.083 0.1641 −32% Metabolite - 4523 50 0.0831 0.1641 −30% Metabolite - 2285 35 0.084 0.1641 −34% 5-hydroxyindoleacetate 50 0.0847 0.1641 −35% Isobar-29-includes-R—S-hydroorotic 35 0.0847 0.1641 −81% acid-5-6-dihydroorotic acid valine 50 0.0849 0.1641 −23% leucine 50 0.0862 0.1651 −37% Metabolite - 4639 50 0.0905 0.1703 −52% 4-hydroxy-3-methoxymandelate 50 0.0906 0.1703 −33% Metabolite - 1656 35 0.0927 0.1726 −52% Metabolite - 2781 35 0.0965 0.1752 −22% 3-phospho-d-glycerate 35 0.0989 0.1752 −33% N-acetyl-L-leucine 35 0.099 0.1752 −33% Metabolite - 4505 50 0.0997 0.1752 −49% Metabolite - 4002 50 0.0999 0.1752 −35% Metabolite - 4112 35 0.1002 0.1752 −33% 4-acetominophen-sulfate 35 0.1013 0.1755 −35% cellobiose 50 0.1027 0.1765 −48% Metabolite - 1829 35 0.1037 0.1767 −22% Metabolite - 3489 35 0.1062 0.1794 102% DL-beta-hydroxyphenylethylamine 35 0.1082 0.1813 −29% Metabolite - 4628 50 0.1098 0.182 −57% 4-hydroxymandelate 50 0.1115 0.182 −39% 5-oxoproline 50 0.1124 0.182 −29% 4-acetamidobutyric acid 35 0.1142 0.182 −28% 4-Guanidinobutanoic acid 35 0.1143 0.182 −28% Metabolite - 2546 35 0.1157 0.182 −23% histidine 50 0.1191 0.182 −38% cysteine 50 0.1196 0.182 −52% Metabolite - 4516 50 0.1197 0.182 −53% L-beta-imidazolelactic acid 50; 35 0.1201 0.182 −25% Metabolite - 2293-possible-O- 35 0.1215 0.182 −32% desmethylvenlafaxine-glucuronide Metabolite - 1383-possible-salicyluric- 35 0.1218 0.182 −52% glucuronide Metabolite - 4027 50 0.1224 0.182 −28% Metabolite - 2807 35 0.1228 0.182 −44% Metabolite - 3576 35 0.1229 0.182 −44% cis-aconitic acid 50 0.124 0.182 −43% Metabolite - 3322 35 0.1266 0.182 −39% N—N-dimethylarginine 35 0.1269 0.182 −23% Metabolite - 3828 35 0.1273 0.182 −32% noradrenaline 50 0.1276 0.182 −43% Metabolite - 4638 50 0.1281 0.182 −49% creatinine 35 0.1327 0.1866 −17% Metabolite - 4618 50 0.1343 0.1868 −32% Metabolite - 4629 50 0.1347 0.1868 −31% Metabolite - 3800 35 0.1366 0.1881 −40% 3-hydroxybutanoic acid 50 0.1415 0.1926 92% Metabolite - 3817 35 0.1418 0.1926 −30% methylmalonic acid 50 0.143 0.193 −42% Metabolite - 4595 50 0.1443 0.1932 −23% Metabolite - 4637 50 0.1468 0.1932 −54% Metabolite - 3830 35 0.1474 0.1932 −40% Metabolite - 4624 50 0.1475 0.1932 −24% xanthine 35 0.148 0.1932 −43% Metabolite - 3805 35 0.1504 0.195 −26% Metabolite - 4635 50 0.1599 0.1957 −56% folic acid 35 0.1603 0.1957 −37% diaminopimelic acid 50; 35 0.1608 0.1957 −26% Metabolite - 2973 50 0.1618 0.1957 29% Metabolite - 3973 35 0.1629 0.1957 −65% Metabolite - 3605 35 0.163 0.1957 −44% N-formyl-L-methionine 35 0.1643 0.1957 −35% urea 50 0.1643 0.1957 −21% riboflavine 35 0.1654 0.1957 −73% Metabolite - 1289 35 0.1665 0.1957 −16% Metabolite - 3380 35 0.1674 0.1957 −29% Metabolite - 4611 50 0.1674 0.1957 −26% Metabolite - 4511 50 0.1685 0.1957 −38% (1′R-1′S)_biopterin 35 0.1687 0.1957 −25% 1-methyladenine 50 0.1694 0.1957 −45% 4-hydroxy-2-quinolinecarboxylic acid 50 0.17 0.1957 −41% Metabolite - 3994 35 0.1716 0.1957 −32% Metabolite - 2550 35 0.1717 0.1957 −44% Metabolite - 3898 35 0.1721 0.1957 −16% Metabolite - 3103 50 0.1723 0.1957 43% possible-L-homocysteine-thiolactone- 50 0.1735 0.1959 −30% identical-to-homocysteine Metabolite - 1122 35 0.1757 0.1972 −23% tryptophan 50; 35 0.1769 0.1972 −20% Metabolite - 1335 35 0.1775 0.1972 −23% Metabolite - 2557-possible- 35 0.1812 0.2002 −34% Pantoprazole-metabolite asparagine 50 0.1833 0.2014 −32% Metabolite - 3837 35 0.1862 0.2025 −33% Metabolite - 3659 35 0.1878 0.2025 −26% mannitol 50 0.1883 0.2025 −39% D-alanyl-D-alanine 35 0.1885 0.2025 −23% 3-amino-isobutyrate 50 0.1894 0.2025 −39% Metabolite - 4498 50 0.1936 0.2059 −27% hypoxanthine 35 0.1978 0.2093 −36% Isobar-24-includes-L-arabitol-adonitol 35 0.2001 0.2095 −8% hydroxyacetic acid 50 0.2012 0.2095 −21% Metabolite - 1843 35 0.2013 0.2095 −18% Metabolite - 3216 35 0.2022 0.2095 −8% Metabolite - 2175 35 0.2045 0.2108 −49% alanine 50 0.2058 0.211 −27% Metabolite - 2366 35 0.2076 0.2118 40% Metabolite - 3873 35 0.2143 0.2124 −23% Metabolite - 4046 50 0.2165 0.2124 −73% Metabolite - 1455 35 0.2166 0.2124 −18% Metabolite - 3708 35 0.2167 0.2124 −63% Metabolite - 3843 35 0.217 0.2124 −30% Metabolite - 3886 35 0.2177 0.2124 −21% Metabolite - 2174 35 0.2178 0.2124 −23% 4-8-dihydroxyquinoline-2-carboxylic 50 0.2182 0.2124 −31% acid Metabolite - 3387 35 0.2183 0.2124 −23% Metabolite - 4271 50 0.2193 0.2124 −30% tyramine 50 0.2204 0.2124 −42% Metabolite - 4496 50 0.2215 0.2124 −15% normetanephrine 50 0.2223 0.2124 −13% Metabolite - 1679 35 0.2229 0.2124 −22% trans-4-hydroxyproline 35; 50 0.2281 0.2163 −24% Metabolite - 2703 35 0.2307 0.2174 −14% Metabolite - 3169 35 0.2314 0.2174 −62% Metabolite - 4634 50 0.2337 0.2185 −28% Metabolite - 3221 35 0.2367 0.2196 −25% alpha-4-dihydroxybenzenepropanoic 50 0.237 0.2196 −48% acid uric acid 35 0.243 0.2227 −6% Metabolite - 3841 35 0.2435 0.2227 −32% Metabolite - 4500 50 0.2437 0.2227 −62% Metabolite - 3667 35 0.2468 0.2245 −20% Metabolite - 4495 50 0.2517 0.228 −22% Metabolite - 3706 35 0.2536 0.2286 −17% D-lyxose 50 0.2626 0.2336 −26% glutamine 50 0.2629 0.2336 −28% Metabolite - 3014 50 0.2637 0.2336 −21% Metabolite - 3802 35 0.2638 0.2336 −34% Metabolite - 2591 35 0.2717 0.2375 32% Metabolite - 3178-possible-NH3-adduct- 35 0.2724 0.2375 −28% of-isobar-42 Metabolite - 2607 35 0.2727 0.2375 −38% L-kynurenine 35 0.2729 0.2375 −76% gamma-L-glutamyl-L-tyrosine 35 0.2752 0.2385 −23% Metabolite - 3832-possible-phenol- 35 0.2771 0.2391 66% sulfate hippuric acid 35 0.2825 0.2427 −21% 4-hydroxyphenylacetate 35; 50 0.2898 0.2469 −24% Metabolite - 3108 50 0.2898 0.2469 −34% Metabolite - 3834 35 0.2916 0.2473 −33% allantoin 35 0.2933 0.2478 −19% Metabolite - 2118 35 0.2957 0.2488 −19% Metabolite - 3440 35 0.2986 0.2501 192% ascorbic acid 50 0.3011 0.2502 −70% Metabolite - 1498 35 0.3011 0.2502 −40% Metabolite - 4509 50 0.3032 0.2508 −38% Isobar-6-includes-valine-betaine 35 0.3058 0.2519 −16% Metabolite - 2150 35 0.312 0.2556 −24% Metabolite - 3911 35 0.3148 0.2556 −14% 1-5-diaminopentane 50 0.3158 0.2556 −40% proline 35; 50 0.3161 0.2556 −29% salicyluric acid 35 0.3167 0.2556 −71% Metabolite - 4494 50 0.3178 0.2556 14% porphobilinogen 35 0.3208 0.2569 −20% Metabolite - 3804 35 0.3263 0.2589 −21% pantothenic acid 35 0.3273 0.2589 −36% Metabolite - 3099 50 0.3281 0.2589 −25% Metabolite - 3660 35 0.3284 0.2589 −39% Metabolite - 3090 50 0.3345 0.2598 −11% Metabolite - 4632 50 0.3356 0.2598 −45% Isobar-2-includes-3-amino-isobutyrate- 35 0.3373 0.2598 183% 2-amino-butyrate-4-aminobutanoic acid- dimethylglycine-choline- Metabolite - 3309 35 0.3374 0.2598 −18% Metabolite - 1110 35 0.3379 0.2598 −17% Metabolite - 3493 35 0.3384 0.2598 −17% Metabolite - 3163-possible- 35 0.3387 0.2598 −15% methylcytidine-benserazide-or-Pyr-Gln- OH N-alpha-acetyl-L-ornithine- 50 0.345 0.2637 −16% Metabolite - 1465 35 0.3552 0.2705 −14% Metabolite - 1834 35 0.3614 0.2729 53% 3-methyl-L-histidine 35 0.3622 0.2729 −14% carnitine 35 0.3625 0.2729 32% Metabolite - 1338 35 0.3638 0.2729 25% dulcitol 50 0.3732 0.2789 −36% glutamic acid 50 0.3771 0.2808 −15% Metabolite - 2259 35 0.3795 0.2815 −42% S-adenosyl-l-homocysteine 35 0.3841 0.2839 −19% D-allose 50 0.3895 0.2858 −21% Metabolite - 4234 35 0.3895 0.2858 −51% Metabolite - 3091 50 0.3975 0.2906 −27% 2-deoxy-D-glucose 50 0.4016 0.292 −26% Metabolite - 3963 35 0.4038 0.292 −54% Metabolite - 2292 35 0.4038 0.292 23% isoleucine 50 0.4077 0.2936 −15% Metabolite - 2893-possible- 35 0.4115 0.2936 −21% demethylated-Rosiglitazone glucose-6-phosphate 50 0.4136 0.2936 189% 2-keto-L-gulonic acid 50 0.4138 0.2936 194% Metabolite - 1496 35 0.4148 0.2936 −31% theobromine-theophylline 35 0.418 0.2944 41% Metabolite - 3131 35 0.42 0.2944 −19% Metabolite - 3085 50 0.4202 0.2944 38% Metabolite - 3893 35 0.4258 0.2971 −15% Metabolite - 2686 35 0.4273 0.2971 −15% Metabolite - 2386 35 0.4286 0.2971 −13% Metabolite - 2108 35 0.432 0.2981 55% Metabolite - 2249 35 0.4331 0.2981 −30% Metabolite - 3604 35 0.4379 0.3004 −16% Metabolite - 3543 35 0.4474 0.3041 −42% Metabolite - 3878 35 0.4474 0.3041 25% Metabolite - 4507 50 0.4492 0.3041 −21% Metabolite - 3771 35 0.4493 0.3041 −28% 3-nitro-L-tyrosine 50; 35 0.4548 0.3062 −20% lactate 50 0.4554 0.3062 14% Metabolite - 3668 35 0.4624 0.3084 12% Metabolite - 2506 35 0.4645 0.3084 −18% Metabolite - 2254 35 0.4659 0.3084 69% Isobar-13-includes-5-keto-D-gluconic 35 0.4661 0.3084 −34% acid-2-keto-L-gulonic acid-D-glucuronic acid Metabolite - 3909 35 0.4663 0.3084 55% Metabolite - 1682 35 0.4794 0.3142 −16% dethiobiotin 50; 35 0.4799 0.3142 −39% alpha-L-sorbopyranose 50 0.482 0.3142 −29% beta-D-lactose 50 0.4838 0.3142 −21% Metabolite - 2329 35 0.4846 0.3142 −11% Metabolite - 4520 50 0.4866 0.3145 32% Metabolite - 1114 35 0.4884 0.3147 −21% malic acid 35 0.49 0.3147 −15% Metabolite - 4501 50 0.4979 0.3188 −19% Metabolite - 3436 35 0.5088 0.3247 −11% Metabolite - 1351 35 0.5117 0.3255 −21% Metabolite - 3806 35 0.5173 0.3281 −24% Metabolite - 2726 35 0.519 0.3281 −12% Metabolite - 4518 50 0.5282 0.3329 −32% Metabolite - 3409 35 0.5303 0.3331 −17% Metabolite - 4133 50 0.5446 0.341 −21% Metabolite - 3952 35 0.547 0.3415 −20% Metabolite - 3879 35 0.5531 0.3442 −27% Metabolite - 3113 50 0.5631 0.3488 17% Metabolite - 3433 35 0.5639 0.3488 −9% tartaric acid 35 0.5703 0.3517 −20% Metabolite - 2698 35 0.5772 0.3549 38% Metabolite - 2056 35 0.5849 0.3582 −8% Metabolite - 3670 35 0.5873 0.3582 −9% Metabolite - 3786 35 0.5899 0.3582 −14% Metabolite - 2853 35 0.5908 0.3582 −16% Metabolite - 3981 35 0.5931 0.3582 −21% Metabolite - 3564 35 0.5948 0.3582 −23% Metabolite - 3781-possible-Na-adduct- 35 0.5951 0.3582 −12% of-Isobar-21 Metabolite - 1186 35 0.5992 0.3585 −11% 2-isopropylmalic acid 50 0.5992 0.3585 37% Isobar-1-includes-mannose-fructose- 35 0.6073 0.3623 26% glucose-galactose-alpha-L- sorbopyranose-Inositol-D-allose Metabolite - 1101 35 0.6174 0.3672 −10% Metabolite - 4167 35 0.6297 0.3734 −10% Metabolite - 2005 35 0.6452 0.3814 −9% sn-Glycerol-3-phosphate 50 0.65 0.3814 −13% 3-methylglutaric acid 35 0.6501 0.3814 9% Metabolite - 3992-possible-Cl-adduct- 35 0.6507 0.3814 −5% of-Formate-dimer mercaptopyruvate 35 0.6565 0.383 −12% homogentisate 35 0.6586 0.383 −9% azelaic acid 35 0.6592 0.383 18% Metabolite - 4510 50 0.6672 0.3866 −13% 3-hydroxyphenylacetate 35 0.674 0.3883 −10% Metabolite - 3127 35 0.6771 0.3883 −9% phosphoenolpyruvate 35 0.6772 0.3883 −17% Metabolite - 3138 35 0.6779 0.3883 −10% agmatine 35 0.6825 0.3898 −15% Metabolite - 3402 35 0.6861 0.3899 −6% alpha-keto-glutarate 35 0.7074 0.4 −11% Metabolite - 4512 50 0.7083 0.4 −17% Metabolite - 2323 35 0.7136 0.4012 −9% Metabolite - 3701 35 0.7145 0.4012 −10% Metabolite - 3016 50 0.73 0.4068 4% Metabolite - 2272 35 0.7302 0.4068 −17% DL-pipecolic acid 35 0.7319 0.4068 −12% Metabolite - 4633 50 0.7325 0.4068 −8% niacinamide 35 0.7378 0.4086 8% Metabolite - 3123 35 0.7434 0.41 −8% galactose 50 0.7512 0.41 −13% pyridoxamine-phosphate 35 0.752 0.41 5% Metabolite - 3101 50 0.7539 0.41 −7% o-phosphoethanolamine 50 0.756 0.41 −8% Metabolite - 3516 35 0.7584 0.41 −7% DL-indole-3-lactic acid 50; 35 0.76 0.41 11% Metabolite - 1126 35 0.7615 0.41 −9% Metabolite - 1981 35 0.7617 0.41 −6% Metabolite - 3986 35 0.7628 0.41 −8% Metabolite - 4598 50 0.7687 0.4121 −7% Metabolite - 4514 50 0.775 0.4127 9% Metabolite - 3507 35 0.7759 0.4127 −7% Metabolite - 1216 35 0.776 0.4127 4% Metabolite - 3053 35 0.788 0.4172 −12% L-rhamnose 50 0.7886 0.4172 5% Metabolite - 2389 35 0.7912 0.4175 6% Metabolite - 1368 35 0.7964 0.4191 −11% alphahydroxybenzeneacetic acid 35 0.7995 0.4196 −4% benzoic acid 50; 35 0.809 0.4235 6% N-acetyl-L-alanine 35 0.8203 0.4259 −4% Metabolite - 4517 50 0.8203 0.4259 −6% Metabolite - 4092 35 0.8218 0.4259 −6% Metabolite - 3977 35 0.8221 0.4259 −8% Isobar-38-includes-N-acetyl-L- 35 0.8281 0.428 4% methionine-5-hydroxy-1H-indole-3- acetic acid Metabolite - 2706 35 0.8316 0.4284 6% Metabolite - 3311-possible-Zolpidem-in- 35 0.8367 0.4284 7% humans- Metabolite - 4010 50 0.8374 0.4284 −6% Metabolite - 3773 35 0.8375 0.4284 −5% Metabolite - 2897 35 0.847 0.4322 5% Metabolite - 3364 35 0.8537 0.434 5% Metabolite - 3231 35 0.8549 0.434 −4% Metabolite - 3957 35 0.8591 0.435 −3% Metabolite - 2269- 35 0.8619 0.4353 −3% Metabolite - 3754 35 0.873 0.4398 5% Metabolite - 2387 35 0.8782 0.4414 −5% Metabolite - 3377 35 0.8819 0.4421 −6% Metabolite - 2319 35 0.8913 0.445 −3% suberic acid 35 0.8921 0.445 3% oxalacetic acid 35 0.8946 0.4451 −2% Metabolite - 3876 35 0.8969 0.4451 −6% Metabolite - 2348 35 0.8988 0.4451 −4% Metabolite - 1839 35 0.9015 0.4453 5% Metabolite - 1113-possible- 35 0.9057 0.4462 −4% acetylcarnitine-or-isopentyl-adenine 3-methyl-2-oxovaleric acid 35 0.9096 0.4469 −4% melibiose 50 0.9114 0.4469 4% Metabolite - 1364 35 0.9316 0.4557 3% Metabolite - 4524 50 0.9364 0.4569 4% thymidine 35 0.9412 0.4581 2% Metabolite - 3755 35 0.9473 0.46 −1% Metabolite - 3855 35 0.9504 0.4604 −1% Metabolite - 3058 50 0.966 0.4638 2% Metabolite - 1116 35 0.9663 0.4638 −1% Metabolite - 3847 35 0.9693 0.4638 −1% Metabolite - 3313 35 0.9709 0.4638 −1% 5-s-methyl-5-thioadenosine 35 0.9714 0.4638 1% Metabolite - 3887 35 0.9799 0.4668 −1% Metabolite - 3824 35 0.9839 0.4676 0% Metabolite - 3457 35 0.9863 0.4676 −1% Metabolite - 1283 35 0.9995 0.4728 0%

Example 3 Distinguish Lower Grade from Higher Grade/Metastatic Prostate Cancer in Subjects Using Plasma

Biomarkers were discovered by (1) analyzing plasma samples from different groups of human subjects to determine the levels of metabolites in the samples and then (2) statistically analyzing the results to determine those metabolites that were differentially present in the two groups.

The plasma samples used for the analysis were from 53 control individuals with negative biopsies for prostate cancer, 43 individuals with lower grade prostate cancer (i.e. Gleason Score major=3) and 15 individuals with aggressive, higher grade prostate cancer (i.e. Gleason Score major=4+). After the levels of metabolites were determined, the data was analyzed using univariate T-tests (i.e., Welch's T-test).

T-tests were used to determine differences in the mean levels of metabolites between two populations (i.e., Lower Grade Prostate cancer vs. Control, Metastatic/High Grade Prostate cancer vs. Control, Metastatic/Higher Grade Prostate cancer vs. Lower Grade Prostate cancer).

Biomarkers:

As listed below in Table 6, biomarkers were discovered that were differentially present between plasma samples from subjects with lower grade prostate cancer and plasma samples from Control subjects with negative prostate biopsies (i.e. not diagnosed with prostate cancer). Table 7 lists biomarkers that were discovered that were differentially present between plasma samples from subjects with metastatic/high grade prostate cancer and plasma samples from Control subjects with biopsy negative prostates (i.e. not diagnosed with prostate cancer). Table 8 lists biomarkers that were discovered that were differentially present between plasma samples from subjects with metastatic/high grade prostate cancer and plasma from subjects with lower grade prostate cancer.

Tables 6-8 include, for each listed biomarker, the p-value and the q-value determined in the statistical analysis of the data concerning the biomarkers and an indication of the percentage difference in the lower grade prostate cancer mean level as compared to the control mean level (Table 6), the metastatic/high grade prostate cancer mean level as compared to the control mean level (Table 7), and the metastatic/high grade prostate cancer mean level as compared to the lower grade prostate cancer mean level (Table 8). The term “Isobar” as used in the tables indicates the compounds that could not be distinguished from each other on the analytical platform used in the analysis (i.e., the compounds in an isobar elute at nearly the same time and have similar (and sometimes exactly the same) quant ions, and thus cannot be distinguished). Library indicates the chemical library that was used to identify the compounds. The number 50 refers to the GC library and the number 35 refers to the LC library.

Biomarkers were discovered by (1) analyzing plasma samples from different groups of human subjects to determine the levels of metabolites in the samples and then (2) statistically analyzing the results to determine those metabolites that were differentially present in the two groups.

The plasma samples used for the analysis were from 53 control individuals with negative biopsies for prostate cancer, 43 individuals with lower grade prostate cancer (i.e. Gleason Score major=3) and 15 individuals with aggressive, high grade prostate cancer (i.e. Gleason Score major=4+). After the levels of metabolites were determined, the data was analyzed using univariate T-tests (i.e., Welch's T-test).

T-tests were used to determine differences in the mean levels of metabolites between two populations (i.e., Lower Grade Prostate cancer vs. Control, Metastatic/High Grade Prostate cancer vs. Control, Metastatic/High Grade Prostate cancer vs. Lower Grade Prostate cancer).

TABLE 6 Plasma Metabolite Biomarkers to distinguish Non-cancer vs. Lower Grade PCA % Change COMP_ID COMPOUND LIB_ID pvalue qvalue in PCA 53 glutamine 50 0.9855 0.9993 −1% 54 tryptophan 50 0.6455 0.9851 3% 57 glutamic acid 50 0.5953 0.9851 −6% 59 histidine 50 0.4258 0.9478 −9% 60 leucine 50 0.2512 0.9478 8% 63 cholesterol 50 0.8251 0.9851 −1% 64 phenylalanine 35 0.61 0.9851 −2% 513 creatinine 35 0.0047 0.5749 −10% 527 lactate 50 0.6496 0.9851 −1% 528 alpha - keto-glutarate 35 0.0081 0.5749 −40% 541 4-hydroxyphenylacetate 35 0.2553 0.9478 −5% 542 3-hydroxybutanoic acid 50 0.748 0.9851 11% 569 caffeine 35 0.0542 0.7407 61% 577 fructose 50 0.2415 0.9478 −26% 581 glucose 50 0.254 0.9478 −4% 584 mannose 50 0.9209 0.9993 1% 594 niacinamide 35 0.7471 0.9851 10% 597 phosphoenolpyruvate 35 0.5253 0.9851 −6% 1105 linoleic acid 50 0.6374 0.9851 3% 1107 allantoin 50 0.6861 0.9851 −8% 1110 arachidonic acid 50 0.4338 0.9478 −6% 1121 heptadecanoic acid 50 0.0432 0.7407 12% 1123 inosine 35 0.8138 0.9851 8% 1125 isoleucine 50 0.2856 0.9478 7% 1126 alanine 50 0.8973 0.9993 −1% 1284 threonine 50 0.9044 0.9993 −1% 1299 tyrosine 50 0.2074 0.9478 8% 1302 methionine 35 0.9843 0.9993 0% 1303 malic acid 35 0.5347 0.9851 −9% 1336 n-hexadecanoic acid 50 0.4029 0.9478 5% 1358 octadecanoic acid 50 0.05 0.7407 7% 1365 tetradecanoic acid 50 0.4504 0.9478 6% 1366 trans-4-hydroxyproline 50 0.8095 0.9851 1% 1413 3-hydroxyphenylacetate 35 0.4804 0.9659 −3% 1414 3-phospho-d-glycerate 35 0.9448 0.9993 −1% 1415 4-amino-5-methyl-2-1H- 35 0.6722 0.9851 3% pyrimidinone 1431 (p-Hydroxyphenyl)lactic acid 50 0.598 0.9851 6% 1432 alphahydroxybenzeneacetic 35 0.8582 0.9851 −2% acid 1437 succinate 50 0.6649 0.9851 −2% 1444 DL-pipecolic acid 35 0.3051 0.9478 −10% 1480 guanidineacetic acid 35 0.3889 0.9478 7% 1493 ornithine 50 0.9114 0.9993 1% 1494 5-oxoproline 50 0.5882 0.9851 4% 1498 N-6-trimethyl-l-lysine 35 0.2178 0.9478 −11% 1507 palmitoleic acid 50 0.5998 0.9851 8% 1508 pantothenic acid 35 0.4151 0.9478 20% 1519 sucrose 50 0.6854 0.9851 6% 1557 3-methylglutaric acid 35 0.1656 0.9478 −9% 1561 alpha - tocopherol 50 0.0145 0.6753 −50% 1564 citric acid 50 0.6977 0.9851 2% 1570 oleic acid 50 0.8649 0.9851 −2% 1572 glyceric acid 50 0.7217 0.9851 2% 1574 histamine 35 0.5568 0.9851 8% 1584 methyl-indole-3-acetate 35 0.9677 0.9993 0% 1587 N-acetyl-L-leucine 35 0.3101 0.9478 31% 1591 N-acetyl-L-valine 35 0.0094 0.5749 20% 1604 uric acid 35 0.9975 0.9993 0% 1643 fumaric acid 50 0.5347 0.9851 −3% 1645 n-dodecanoate 50 0.7836 0.9851 3% 1648 serine 50 0.2503 0.9478 8% 1649 valine 50 0.3429 0.9478 7% 1670 urea 50 0.5816 0.9851 4% 1708 7-8-dihydrofolic acid 35 0.3763 0.9478 30% 1898 proline 50 0.6963 0.9851 −3% 2078 pyrophosphate 35 0.97 0.9993 0% 2092 catechol 35 0.9928 0.9993 0% 2132 citrulline 50 0.7476 0.9851 −3% 2730 gamma - L-glutamyl-L- 35 0.8715 0.9852 −3% glutamine 2734 gamma - L-glutamyl-L- 35 0.2947 0.9478 6% tyrosine 2832 adenosine-5-monophosphate 35 0.661 0.9851 −4% 2848 guanosine-5-diphosphate 35 0.9286 0.9993 1% 3127 hypoxanthine 35 0.6226 0.9851 −6% 3138 pyridoxamine-phosphate 35 0.1559 0.9478 16% 3147 xanthine 35 0.4283 0.9478 −10% 4966 xylitol 35 0.6274 0.9851 5% 5280 biliverdin 35 0.2685 0.9478 33% 5331 pyridoxal-phosphate 35 0.2348 0.9478 −5% 5618 Metabolite - 1085-possible- 35 0.4282 0.9478 4% isolobinine-or-4-aminoestra- 1-3-5-10-triene-3-17beta-diol 5628 Metabolite - 1086 35 0.8863 0.9931 −2% 5669 Metabolite - 1104 35 0.7565 0.9851 −1% 5687 Metabolite - 1110 35 0.8421 0.9851 5% 5689 Metabolite - 1111-possible- 35 0.1327 0.9478 11% methylnitronitrosoguanidine- or-ethyl-thiocarbamoylacetate 5697 acetylcarnitine- 35 0.8273 0.9851 −2% 5717 Metabolite - 1121 35 0.2772 0.9478 10% 5733 Metabolite - 1127 35 0.0093 0.5749 −18% 5765 Metabolite - 1142-possible-5- 35 0.7839 0.9851 9% hydroxypentanoate-or-beta- hydroxyisovaleric acid 5788 Metabolite - 1183 35 0.3488 0.9478 145% 5792 Metabolite - 1185 35 0.001 0.3826 35% 5800 Metabolite - 1188 35 0.0412 0.7407 36% 6112 Metabolite - 1203-HXGXX- 35 0.3191 0.9478 53% in-MTRX 6130 Metabolite - 1208 35 0.7504 0.9851 7% 6136 Metabolite - 1211-possible- 35 0.0416 0.7407 287% IHWESASLLR- 6144 Metabolite - 1215 35 0.9559 0.9993 2% 6147 Metabolite - 1216 35 0.6032 0.9851 8% 6155 Metabolite - 1220 35 0.8003 0.9851 3% 6171 Metabolite - 1244 35 0.3509 0.9478 11% 6266 Metabolite - 1286 35 0.3935 0.9478 5% 6278 Metabolite - 1289 35 0.1436 0.9478 −16% 6362 Metabolite - 1323-possible-p- 35 0.3987 0.9478 17% cresol-sulfate 6398 Metabolite - 1335 35 0.9275 0.9993 −1% 6413 Metabolite - 1342-possible- 35 0.3835 0.9478 16% phenylacetylglutamine-or- formyl-N-acetyl-5- methoxykynurenamine 6437 Metabolite - 1349-possible-N- 35 0.9654 0.9993 1% acetyl-8-O-methyl-Neuraminic acid 6443 Metabolite - 1351 35 0.9312 0.9993 −1% 6537 Metabolite - 1389-possible- 35 0.3189 0.9478 308% gemfibrozil-glucuronide- 6549 Metabolite - 1392 35 0.4102 0.9478 12% 6787 Metabolite - 1465 35 0.7835 0.9851 5% 6852 Metabolite - 1498 35 0.1492 0.9478 −14% 6987 Metabolite - 1573 35 0.8168 0.9851 −2% 7029 Metabolite - 1597 35 0.0206 0.7407 10% 7081 Metabolite - 1609 35 0.3186 0.9478 8% 7177 Metabolite - 1656 35 0.8442 0.9851 2% 7359 n-acetyl-L-aspartic acid 35 0.7069 0.9851 −2% 7446 p-hydroxybenzaldehyde 35 0.3568 0.9478 −6% 7595 Metabolite - 1817 35 0.6472 0.9851 3% 7639 oxalic acid 35 0.7835 0.9851 2% 7644 Metabolite - 1831-possible-Cl- 35 0.2483 0.9478 −29% adduct-of-citrulline 7650 Metabolite - 1834 35 0.6754 0.9851 −8% 7652 Metabolite - 1835 35 0.031 0.7407 25% 7654 Metabolite - 1836 35 0.7242 0.9851 −3% 7660 Metabolite - 1839 35 0.8404 0.9851 −4% 7672 Metabolite - 1843 35 0.7507 0.9851 3% 7933 Metabolite - 1911 35 0.0739 0.7639 48% 7935 paraxanthine 35 0.1117 0.9478 38% 7941 Metabolite - 1914 35 0.8081 0.9851 6% 7944 Metabolite - 1915 35 0.3455 0.9478 55% 7957 trans-2-3-4- 35 0.8125 0.9851 5% trimethoxycinnamic acid 8091 glycocholic acid 35 0.2546 0.9478 30% 8176 Metabolite - 1974 35 0.4143 0.9478 10% 8189 Metabolite - 1977 35 0.8558 0.9851 2% 8196 Metabolite - 1979-Cl-adduct- 35 0.5336 0.9851 −5% of-isobar-19 8217 Metabolite - 1983 35 0.1187 0.9478 410% 8300 Metabolite - 1988 35 0.1698 0.9478 −14% 8336 Metabolite - 2005 35 0.8806 0.9897 −2% 8404 Metabolite - 2027 35 0.6346 0.9851 −4% 8649 Metabolite - 2053 35 0.2084 0.9478 17% 8669 Metabolite - 2055 35 0.9233 0.9993 1% 8677 Metabolite - 2056 35 0.5835 0.9851 6% 8796 Metabolite - 2074 35 0.8013 0.9851 −2% 8959 Metabolite - 2100 35 0.4481 0.9478 5% 9007 Metabolite - 2108 35 0.9789 0.9993 0% 9024 Metabolite - 2111 35 0.6202 0.9851 −5% 9092 Metabolite - 2129 35 0.5701 0.9851 −9% 9106 Metabolite - 2130 35 0.4394 0.9478 −12% 9130 Metabolite - 2139 35 0.7305 0.9851 3% 9137 Metabolite - 2141 35 0.5077 0.9851 12% 9491 Metabolite - 2185 35 0.0387 0.7407 18% 9748 Metabolite - 2212 35 0.3479 0.9478 −9% 10087 Metabolite - 2249 35 0.6712 0.9851 −4% 10092 Metabolite - 2250 35 0.0564 0.7407 63% 10122 Metabolite - 2254 35 0.4991 0.9851 −15% 10143 Metabolite - 2255- 35 0.0474 0.7407 89% hydroxyproline-form-of- bradykinin 10145 Metabolite - 2256 35 0.1134 0.9478 −18% 10245 Metabolite - 2269- 35 0.7452 0.9851 8% 10317 Metabolite - 2279 35 0.2635 0.9478 32% 10327 Metabolite - 2281 35 0.5771 0.9851 34% 10378 Metabolite - 2287 35 0.395 0.9478 59% 10438 gamma-glu-leu 35 0.4307 0.9478 4% 10461 Metabolite - 2313 35 0.71 0.9851 −4% 10476 Metabolite - 2316 35 0.456 0.9478 −13% 10544 Metabolite - 2329 35 0.8979 0.9993 1% 10551 Metabolite - 2347 35 0.6776 0.9851 8% 10604 Metabolite - 2370 35 0.6853 0.9851 4% 10629 Metabolite - 2386 35 0.8269 0.9851 −4% 10644 Metabolite - 2387 35 0.5459 0.9851 52% 10655 Metabolite - 2388 35 0.7697 0.9851 −1% 10667 Metabolite - 2389 35 0.318 0.9478 20% 10672 Metabolite - 2390 35 0.4169 0.9478 23% 10692 Metabolite - 2391 35 0.397 0.9478 −6% 10698 Metabolite - 2392 35 0.7279 0.9851 −9% 10737 Isobar-1-includes-mannose- 35 0.7112 0.9851 4% fructose-glucose-galactose- alpha - L-sorbopyranose- Inositol-D-allose-D--altrose-D- psicone 10739 Metabolite - 2407 35 0.9869 0.9993 0% 10741 Isobar-2-includes-2- 35 0.6711 0.9851 −10% aminoisobutyric acid-3-amino- isobutyrate-2-amino-butyrate- 4-aminobutanoic acid- dimethylglycine-choline- 10743 Isobar-4-includes-Gluconic 35 0.8686 0.9851 −2% acid-DL-arabinose-D-ribose-L- xylose-DL-lyxose-D-xylulose 10744 Isobar-5-includes-asparagine- 35 0.7225 0.9851 4% ornithine-gly-gly 10746 Isobar-6-includes-valine- 35 0.1667 0.9478 5% betaine 10753 Isobar-9-includes-galactinol- 35 0.6051 0.9851 −7% dihydrate-turanose-kojibiose- D-leucrose-lactulose- sophorose-sucrose-beta-D- lactose-D-trehalose-D- cellobiose-D-Maltose- palatinose-melibiose-alpha - D-lactose 10782 Metabolite - 2486 35 0.5968 0.9851 8% 10785 Metabolite - 2506 35 0.9224 0.9993 2% 10787 Metabolite - 2507 35 0.6105 0.9851 −13% 10825 Metabolite - 2546 35 0.9993 0.9993 0% 11053 Metabolite - 2567 35 0.1285 0.9478 9% 11111 Metabolite - 2592 35 0.4637 0.9582 54% 11219 Metabolite - 2686 35 0.5844 0.9851 3% 11222 Metabolite - 2688 35 0.163 0.9478 14% 11323 Metabolite - 2711 35 0.9889 0.9993 0% 11438 phosphate 50 0.8104 0.9851 0% 11499 Metabolite - 2753 35 0.4215 0.9478 7% 11777 glycine 50 0.3338 0.9478 −8% 11813 Metabolite - 2809 35 0.8323 0.9851 3% 12035 nonanate 50 0.7544 0.9851 1% 12109 Metabolite - 2853 35 0.0277 0.7407 39% 12298 Metabolite - 2867 35 0.1237 0.9478 −45% 12478 Metabolite - 2898 35 0.0495 0.7407 −51% 12532 Metabolite - 2914 50 0.1166 0.9478 2% 12533 Metabolite - 2915 50 0.2615 0.9478 −7% 12543 2-hydroxy-butanoic acid 50 0.1956 0.9478 17% 12562 Metabolite - 2955 50 0.279 0.9478 3% 12593 Metabolite - 2973 50 0.9818 0.9993 0% 12594 Metabolite - 2974 50 0.9799 0.9993 0% 12601 Metabolite - 2978 50 0.6254 0.9851 3% 12625 Metabolite - 3002 50 0.9162 0.9993 −2% 12626 Metabolite - 3003 50 0.1382 0.9478 −9% 12627 Metabolite - 3004 50 0.5311 0.9851 −5% 12639 Metabolite - 3012 50 0.4723 0.9653 5% 12641 meso-erythritol 50 0.3847 0.9478 −7% 12644 Metabolite - 3016 50 0.1293 0.9478 −7% 12645 Metabolite - 3017 50 0.0303 0.7407 18% 12647 Metabolite - 3019 50 0.2488 0.9478 7% 12648 Metabolite - 3020 50 0.4953 0.9851 6% 12650 Metabolite - 3022 50 0.9679 0.9993 0% 12656 Metabolite - 3025 50 0.195 0.9478 8% 12658 Metabolite - 3026 50 0.3559 0.9478 7% 12663 Metabolite - 3030 50 0.0934 0.9247 9% 12666 Metabolite - 3033 50 0.8631 0.9851 −1% 12673 Metabolite - 3040 50 0.3373 0.9478 10% 12682 Metabolite - 3044 35 0.1918 0.9478 12% 12719 Metabolite - 3055 35 0.741 0.9851 5% 12720 Metabolite - 3056 35 0.5288 0.9851 −4% 12726 Metabolite - 3058 50 0.727 0.9851 3% 12739 1-5-anhydro-D-glucitol 50 0.2751 0.9478 −8% 12751 Metabolite - 3073 50 0.921 0.9993 1% 12753 Metabolite - 3074 50 0.3835 0.9478 −22% 12754 Metabolite - 3075 50 0.2497 0.9478 −7% 12756 Metabolite - 3077 50 0.9463 0.9993 1% 12757 Metabolite - 3078 50 0.8351 0.9851 −2% 12761 Metabolite - 3081 50 0.0685 0.7493 −9% 12765 inositol 50 0.4516 0.9478 −5% 12768 Metabolite - 3088 50 0.1228 0.9478 −15% 12769 Metabolite - 3089 50 0.0654 0.7493 16% 12771 Metabolite - 3091 50 0.25 0.9478 21% 12773 Metabolite - 3093 50 0.4676 0.961 9% 12774 Metabolite - 3094 50 0.9437 0.9993 0% 12777 Metabolite - 3097 50 0.2879 0.9478 16% 12780 Metabolite - 3098 50 0.1558 0.9478 12% 12781 Metabolite - 3099 50 0.0718 0.7631 26% 12784 Metabolite - 3102 50 0.8378 0.9851 −1% 12790 Metabolite - 3108 50 0.6368 0.9851 2% 12876 Metabolite - 3125 35 0.4896 0.9793 −4% 12912 Metabolite - 3129 35 0.4506 0.9478 9% 12924 Metabolite - 3131 35 0.6823 0.9851 5% 12931 DL-hexanoyl-carnitine 35 0.6978 0.9851 3% 12960 Metabolite - 3134 35 0.9924 0.9993 0% 12969 Metabolite - 3135 35 0.5304 0.9851 13% 13018 Metabolite - 3138 35 0.0971 0.9247 −16% 13038 Metabolite - 3143 35 0.9259 0.9993 1% 13065 Metabolite - 3146 35 0.8545 0.9851 4% 13104 Metabolite - 3160 35 0.2622 0.9478 −10% 13142 Metabolite - 3165 35 0.3363 0.9478 −5% 13146 Metabolite - 3166 35 0.8043 0.9851 4% 13148 Metabolite - 3167 35 0.044 0.7407 21% 13179 possible-Metabolite - 3176- 35 0.7152 0.9851 5% possible-creatine 13208 Metabolite - 3181 35 0.354 0.9478 −9% 13211 Metabolite - 3182 35 0.9963 0.9993 0% 13214 Metabolite - 3183-possible- 35 0.7525 0.9851 2% gamma-L-glutamyl-L- phenylalanine 13217 Metabolite - 3184 35 0.1642 0.9478 −11% 13249 Metabolite - 3215 35 0.6878 0.9851 −4% 13251 Metabolite - 3216 35 0.2268 0.9478 −13% 13257 Metabolite - 3218 35 0.5081 0.9851 5% 13342 Metabolite - 3243 35 0.5847 0.9851 10% 13448 Metabolite - 3303 35 0.0978 0.9247 −9% 13459 Metabolite - 3305 35 0.3143 0.9478 −19% 13484 Metabolite - 3309 35 0.0626 0.7493 −25% 13505 Metabolite - 3313 35 0.5629 0.9851 −23% 13509 Metabolite - 3314 35 0.2956 0.9478 −8% 13534 Metabolite - 3320-possible- 35 0.7791 0.9851 10% pimpinellin-or- tetrahydroxybenzophenone 13545 Metabolite - 3322 35 0.2266 0.9478 16% 13589 Metabolite - 3327 35 0.831 0.9851 −4% 13775 Metabolite - 3370 35 0.8025 0.9851 1% 13803 Metabolite - 3377 35 0.2963 0.9478 −17% 13904 Metabolite - 3402 35 0.3454 0.9478 −15% 14027 Metabolite - 3426 35 0.1435 0.9478 −3% 14084 Metabolite - 3436 35 0.2313 0.9478 13% 14117 Metabolite - 3441 35 0.2102 0.9478 −10% 14239 Metabolite - 3474 35 0.3092 0.9478 −9% 14249 Metabolite - 3476 35 0.3874 0.9478 −16% 14368 Metabolite - 3489 35 0.383 0.9478 −19% 14439 Metabolite - 3498 35 0.7901 0.9851 3% 14495 Metabolite - 3534 35 0.2653 0.9478 13% 14595 Metabolite - 3576 35 0.6382 0.9851 −5% 14608 Metabolite - 3578 35 0.243 0.9478 13% 14639 Metabolite - 3603 35 0.04 0.7407 19% 14640 Metabolite - 3604 35 0.2069 0.9478 30% 14672 Metabolite - 3615 35 0.275 0.9478 15% 14715 Metabolite - 3653-possible- 35 0.6884 0.9851 −12% stachydrine- 14766 Metabolite - 3670 35 0.1501 0.9478 8% 14785 isobar-glycochenodeoxycholic 35 0.3915 0.9478 18% acid-glycodeoxycholic acid 14787 Metabolite - 3698 35 0.3714 0.9478 −11% 14837 Metabolite - 3707 35 0.4795 0.9659 18% 14961 Metabolite - 3752 35 0.0597 0.7407 228% 15000 Metabolite - 3758 35 0.3269 0.9478 −23% 15017 Metabolite - 3761 35 0.6023 0.9851 −5% 15032 Metabolite - 3765 35 0.0289 0.7407 43% 15063 Metabolite - 3772 35 0.573 0.9851 −4% 15113 Metabolite - 3783 35 0.983 0.9993 0% 15122 glycerol 50 0.7135 0.9851 −2% 15128 DL-homocysteine 35 0.1339 0.9478 −14% 15129 D-alanyl-D-alanine 35 0.0165 0.6833 23% 15211 Metabolite - 3807 35 0.3174 0.9478 −5% 15220 Metabolite - 3813 35 0.6421 0.9851 −5% 15227 Metabolite - 3816 35 0.9802 0.9993 0% 15251 Metabolite - 3830 35 0.262 0.9478 20% 15253 Metabolite - 3832-possible- 35 0.0596 0.7407 −45% phenol-sulfate 15278 Metabolite - 3843 35 0.0092 0.5749 −16% 15319 DL-phenyllactic acid 35 0.2331 0.9478 32% 15326 Metabolite - 3879 35 0.5692 0.9851 13% 15328 azelaic acid 35 0.7398 0.9851 −2% 15336 tartaric acid 35 0.9993 0.9993 0% 15365 sn-Glycerol-3-phosphate 50 0.4116 0.9478 3% 15389 Metabolite - 3900 35 0.5829 0.9851 3% 15500 carnitine 35 0.528 0.9851 3% 15529 Metabolite - 3951 35 0.0399 0.7407 −11% 15535 Metabolite - 3955 35 0.5955 0.9851 −7% 15606 Metabolite - 3968 35 0.761 0.9851 −5% 15612 Metabolite - 3972 35 0.2908 0.9478 8% 15626 Metabolite - 3977 35 0.2184 0.9478 −7% 15677 3-methyl-L-histidine 35 0.1203 0.9478 −8% 15681 4-Guanidinobutanoic acid 35 0.2211 0.9478 −14% 15683 4-methyl-2-oxopentanoate 50 0.4541 0.9478 5% 15704 heptanedioic acid 35 0.8664 0.9851 −2% 15744 N-N-dimethylarginine 35 0.6586 0.9851 3% 15753 hippuric acid 35 0.6181 0.9851 10% 15991 L-alpha - 35 0.8467 0.9851 −3% glycerophosphorylcholine 16002 Metabolite - 3992-possible-Cl- 35 0.874 0.9852 −1% adduct-of-Formate-dimer 16016 Metabolite - 3994 35 0.7041 0.9851 −8% 16071 Metabolite - 4020 50 0.3935 0.9478 5% 16091 Metabolite - 4031-possible- 35 0.94 0.9993 −1% norlevorphenol- isobutylphendienamide- amprolium 16107 lysine 50 0.8238 0.9851 2% 16137 Metabolite - 4078 35 0.5038 0.9851 −12% 16161 gamma - glutamyl-glutamic 35 0.5112 0.9851 8% acid 16186 Metabolite - 4096-possible- 35 0.2257 0.9478 18% gamma - glu-gly-leu- 16226 Isobar-28-includes-L- 35 0.6861 0.9851 3% threonine-L-allothreonine-L- homoserine-S-4-amino-2- hydroxybutyric acid 16231 Isobar-20-includes-fumaric 35 0.2502 0.9478 18% acid-3-methyl-2-oxobutanoate 16232 Isobar-17-includes-arginine-N- 35 0.9455 0.9993 −1% alpha - acetyl-ornithine 16233 Isobar-13-includes-5-keto-D- 35 0.799 0.9851 3% gluconic acid-2-keto-L-gulonic acid-D-glucuronic acid-D- galacturonic acid 16235 Isobar-19-includes-D-saccharic 35 0.8329 0.9851 3% acid-1-5-anhydro-D-glucitol-2- deoxy-D-galactose-2-deoxy-D- glucose-L-fucose-L-rhamnose 16237 Isobar-25-includes-L-gulono-1- 35 0.8393 0.9851 −3% 4-lactone-glucono-gamma - lactone- 16241 Isobar-30-includes- 35 0.282 0.9478 11% maltotetraose-stachyose 16243 L-kynurenine 35 0.6848 0.9851 2% 16244 Isobar-21-includes-gamma - 35 0.0676 0.7493 −21% aminobutyryl-L-histidine-L- anserine 16246 Isobar-18-includes-D-fructose- 35 0.6754 0.9851 5% 1-phosphate-beta - D-fructose- 6-phosphate 16279 Isobar-36-includes-D-sorbitol- 35 0.0432 0.7407 18% 6-phosphate-mannitol-1- phosphate 16290 Metabolite - 4133 50 0.1746 0.9478 −9% 16308 Metabolite - 4147 50 0.6922 0.9851 −4% 16330 Metabolite - 4163 35 0.4304 0.9478 −10% 16337 Metabolite - 4167 35 0.7076 0.9851 −3% 16462 Metabolite - 4234 35 0.4466 0.9478 −5% 16471 Metabolite - 4238 35 0.5384 0.9851 10% 16508 Metabolite - 4272 50 0.1526 0.9478 −11% 16621 Metabolite - 4355 50 0.3937 0.9478 −17% 16653 Metabolite - 4361 50 0.0994 0.9247 21% 16666 Metabolite - 4365 50 0.2946 0.9478 −10% 16824 iminodiacetic acid 50 0.7755 0.9851 −3% 16829 Metabolite - 4503 50 0.9392 0.9993 −1% 16848 Metabolite - 4511 50 0.8497 0.9851 −2% 16952 Metabolite - 4593 50 0.3035 0.9478 4% 17028 Metabolite - 4611 50 0.3813 0.9478 −5% 17328 Metabolite - 4768 50 0.4803 0.9659 13% 17330 Metabolite - 4769 50 0.2485 0.9478 14% 17359 Metabolite - 4791 50 0.8127 0.9851 −5% 17388 Metabolite - 4795 50 0.2822 0.9478 11% 17614 Metabolite - 4966 50 0.33 0.9478 9% 17627 Metabolite - 4986 50 0.0582 0.7407 28% 18118 Metabolite - 5346 50 0.4123 0.9478 6% 18146 Metabolite - 5366 50 0.0108 0.5749 40% 18232 Metabolite - 5403 50 0.3291 0.9478 6% 18316 Metabolite - 5437 50 0.1177 0.9478 30% 18335 D-quinic acid 50 0.6344 0.9851 10% 18349 DL-indole-3-lactic acid 50 0.6042 0.9851 −4% 18868 Metabolite - 5847 50 0.8544 0.9851 3% 18926 Metabolite - 5906 50 0.9154 0.9993 4% 18929 Metabolite - 5907 50 0.2112 0.9478 8%

TABLE 7 Plasma Metabolite Biomarkers to distinguish Non-cancer from Higher Grade PCA. % Change COMP_ID COMPOUND LIB_ID p-value q-value in PCA 53 glutamine 50 0.1993 0.3977 −14% 54 tryptophan 50 0.1049 0.3151 −10% 57 glutamic acid 50 0.1716 0.3828 −18% 59 histidine 50 0.2197 0.4209 −11% 60 leucine 50 0.0833 0.2916 −13% 63 cholesterol 50 0.1481 0.3699 −10% 64 phenylalanine 35 0.05 0.245 −7% 513 creatinine 35 0.0563 0.2564 −10% 527 lactate 50 0.0035 0.0655 −15% 528 alpha - keto-glutarate 35 0.0099 0.0976 −45% 541 4-hydroxyphenylacetate 35 0.3644 0.481 −5% 542 3-hydroxybutanoic acid 50 0.0434 0.221 −45% 569 caffeine 35 0.0049 0.0777 −51% 577 fructose 50 0.0544 0.2523 −41% 581 glucose 50 0.1835 0.3851 −6% 584 mannose 50 0.9521 0.6641 0% 594 niacinamide 35 0.6135 0.59 −11% 597 phosphoenolpyruvate 35 0.4146 0.5151 −9% 1105 linoleic acid 50 0.0622 0.2681 −14% 1107 allantoin 50 0.4785 0.5344 −14% 1110 arachidonic acid 50 0.3429 0.481 −12% 1121 heptadecanoic acid 50 0.0029 0.0624 −19% 1123 inosine 35 0.7999 0.6385 10% 1125 isoleucine 50 0.072 0.2829 −13% 1126 alanine 50 0.0821 0.2916 −16% 1284 threonine 50 0.2588 0.4682 −9% 1299 tyrosine 50 0.6026 0.5883 −4% 1302 methionine 35 0.499 0.5413 −4% 1303 malic acid 35 0.343 0.481 −21% 1336 n-hexadecanoic acid 50 0.0078 0.0976 −16% 1358 octadecanoic acid 50 0.0337 0.1829 −10% 1365 tetradecanoic acid 50 0.0076 0.0976 −17% 1366 trans-4-hydroxyproline 50 0.0164 0.1319 −14% 1413 3-hydroxyphenylacetate 35 0.9038 0.6568 −1% 1414 3-phospho-d-glycerate 35 0.3849 0.4882 −13% 1415 4-amino-5-methyl-2-1H- 35 0.4688 0.5282 −8% pyrimidinone 1431 (p-Hydroxyphenyl)lactic acid 50 0.0618 0.2681 −22% 1432 alphahydroxybenzeneacetic acid 35 0.9337 0.6641 1% 1437 succinate 50 0.3397 0.481 7% 1444 DL-pipecolic acid 35 0.3851 0.4882 −10% 1480 guanidineacetic acid 35 0.7214 0.6178 5% 1493 ornithine 50 0.1892 0.3889 −18% 1494 5-oxoproline 50 0.1956 0.3977 −9% 1498 N-6-trimethyl-l-lysine 35 0.6864 0.6107 −6% 1507 palmitoleic acid 50 4.00E−04 0.0178 −40% 1508 pantothenic acid 35 0.3718 0.481 −17% 1519 sucrose 50 0.8433 0.6512 4% 1557 3-methylglutaric acid 35 0.0249 0.1479 −18% 1561 alpha -tocopherol 50 0.0158 0.1319 −53% 1564 citric acid 50 0.4573 0.5239 −4% 1570 oleic acid 50 0.0021 0.0503 −35% 1572 glyceric acid 50 0.1532 0.3719 20% 1574 histamine 35 0.8493 0.6519 4% 1584 methyl-indole-3-acetate 35 0.28 0.4695 −13% 1587 N-acetyl-L-leucine 35 0.71 0.6136 −10% 1591 N-acetyl-L-valine 35 0.0093 0.0976 21% 1604 uric acid 35 0.7956 0.6385 −1% 1643 fumaric acid 50 0.4015 0.5016 −9% 1645 n-dodecanoate 50 0.187 0.3875 −12% 1648 serine 50 0.3365 0.481 −7% 1649 valine 50 0.0224 0.1466 −14% 1670 urea 50 0.3643 0.481 7% 1708 7-8-dihydrofolic acid 35 0.9239 0.6628 4% 1898 proline 50 0.1339 0.3538 −19% 2078 pyrophosphate 35 0.7732 0.6377 −7% 2092 catechol 35 0.259 0.4682 −31% 2132 citrulline 50 0.4546 0.5239 6% 2730 gamma - L-glutamyl-L- 35 0.552 0.5628 12% glutamine 2734 gamma - L-glutamyl-L-tyrosine 35 0.5344 0.5577 4% 2832 adenosine-5-monophosphate 35 0.1631 0.3779 −17% 2848 guanosine-5-diphosphate 35 0.5329 0.5577 −8% 3127 hypoxanthine 35 0.7241 0.6178 −5% 3138 pyridoxamine-phosphate 35 0.8654 0.6522 2% 3147 xanthine 35 0.9538 0.6641 2% 4966 xylitol 35 0.3179 0.481 18% 5280 biliverdin 35 0.4282 0.5189 −11% 5331 pyridoxal-phosphate 35 0.3699 0.481 4% 5618 Metabolite - 1085-possible- 35 0.0247 0.1479 21% isolobinine-or-4-aminoestra - 1- 3-5-10-triene-3-17beta - diol 5628 Metabolite - 1086 35 0.5566 0.5628 −10% 5669 Metabolite - 1104 35 0.0086 0.0976 −15% 5687 Metabolite - 1110 35 0.2792 0.4695 45% 5689 Metabolite - 1111-possible- 35 0.9467 0.6641 1% methylnitronitrosoguanidine-or- ethyl-thiocarbamoylacetate 5697 acetylcarnitine- 35 0.2254 0.4256 −12% 5717 Metabolite - 1121 35 0.0166 0.1319 29% 5733 Metabolite - 1127 35 6.00E−04 0.0178 −35% 5765 Metabolite - 1142-possible-5- 35 0.611 0.59 −11% hydroxypentanoate-or-beta - hydroxyisovaleric acid 5788 Metabolite - 1183 35 0.7587 0.6319 10% 5792 Metabolite - 1185 35 0.0012 0.034 68% 5800 Metabolite - 1188 35 0.2872 0.4722 18% 6112 Metabolite - 1203-HXGXX - in- 35 0.3875 0.4889 −20% MTRX 6130 Metabolite - 1208 35 0.5109 0.547 −15% 6136 Metabolite - 1211-possible- 35 0.2482 0.4551 204% IHWESASLLR- 6144 Metabolite - 1215 35 0.7273 0.6178 12% 6147 Metabolite - 1216 35 0.4342 0.5189 −8% 6155 Metabolite - 1220 35 0.8726 0.6522 3% 6171 Metabolite - 1244 35 0.7307 0.6186 −6% 6266 Metabolite - 1286 35 0.021 0.1462 16% 6278 Metabolite - 1289 35 0.8015 0.6385 −4% 6362 Metabolite - 1323-possible-p- 35 0.5645 0.5664 17% cresol-sulfate 6398 Metabolite - 1335 35 0.9375 0.6641 1% 6413 Metabolite - 1342-possible- 35 0.7405 0.6228 7% phenylacetylglutamine-or- formyl-N-acetyl-5- methoxykynurenamine 6437 Metabolite - 1349-possible-N- 35 0.9989 0.6849 0% acetyl-8-O-methyl-Neuraminic acid 6443 Metabolite - 1351 35 0.7988 0.6385 −3% 6537 Metabolite - 1389-possible- 35 0.5683 0.5664 −11% gemfibrozil-glucuronide- 6549 Metabolite - 1392 35 0.8385 0.6512 −5% 6787 Metabolite - 1465 35 0.7433 0.6231 −4% 6852 Metabolite - 1498 35 0.3324 0.481 15% 6987 Metabolite - 1573 35 0.8266 0.6482 −4% 7029 Metabolite - 1597 35 0.6293 0.6006 3% 7081 Metabolite - 1609 35 0.118 0.327 −16% 7177 Metabolite - 1656 35 0.3641 0.481 15% 7359 n-acetyl-L-aspartic acid 35 0.0139 0.1266 −23% 7446 p-hydroxybenzaldehyde 35 0.3129 0.481 −9% 7595 Metabolite - 1817 35 0.0951 0.3035 24% 7639 oxalic acid 35 0.0942 0.3035 14% 7644 Metabolite - 1831-possible-Cl- 35 0.026 0.1507 −61% adduct-of-citrulline 7650 Metabolite - 1834 35 0.0722 0.2829 −35% 7652 Metabolite - 1835 35 0.4205 0.5151 11% 7654 Metabolite - 1836 35 0.0891 0.295 −28% 7660 Metabolite - 1839 35 0.0218 0.1462 −45% 7672 Metabolite - 1843 35 0.9787 0.6778 −1% 7933 Metabolite - 1911 35 0.4204 0.5151 25% 7935 paraxanthine 35 0.0203 0.1462 −41% 7941 Metabolite - 1914 35 0.3503 0.481 32% 7944 Metabolite - 1915 35 0.8018 0.6385 15% 7957 trans-2-3-4-trimethoxycinnamic 35 0.3149 0.481 35% acid 8091 glycocholic acid 35 0.9047 0.6568 −5% 8176 Metabolite - 1974 35 0.8572 0.6522 4% 8189 Metabolite - 1977 35 0.1838 0.3851 −12% 8196 Metabolite - 1979-Cl-adduct-of- 35 0.9459 0.6641 1% isobar-19 8217 Metabolite - 1983 35 0.3016 0.481 246% 8300 Metabolite - 1988 35 0.01 0.0976 −30% 8336 Metabolite - 2005 35 0.0359 0.1905 −26% 8404 Metabolite - 2027 35 0.1676 0.3788 −14% 8649 Metabolite - 2053 35 0.7092 0.6136 −5% 8669 Metabolite - 2055 35 0.8342 0.6512 4% 8677 Metabolite - 2056 35 0.1623 0.3779 −15% 8796 Metabolite - 2074 35 0.9322 0.6641 2% 8959 Metabolite - 2100 35 0.6011 0.5883 4% 9007 Metabolite - 2108 35 0.0055 0.0777 −29% 9024 Metabolite - 2111 35 0.3265 0.481 −15% 9092 Metabolite - 2129 35 0.8607 0.6522 −4% 9106 Metabolite - 2130 35 0.0964 0.3035 72% 9130 Metabolite - 2139 35 0.5099 0.547 7% 9137 Metabolite - 2141 35 0.0052 0.0777 −43% 9491 Metabolite - 2185 35 0.6878 0.6107 4% 9748 Metabolite - 2212 35 0.2255 0.4256 19% 10087 Metabolite - 2249 35 0.6409 0.6006 −6% 10092 Metabolite - 2250 35 0.4987 0.5413 18% 10122 Metabolite - 2254 35 0.1171 0.327 −39% 10143 Metabolite - 2255- 35 0.1844 0.3851 139% hydroxyproline-form-of- bradykinin 10145 Metabolite - 2256 35 0.862 0.6522 −3% 10245 Metabolite - 2269- 35 0.2698 0.4695 34% 10317 Metabolite - 2279 35 0.3109 0.481 −24% 10327 Metabolite - 2281 35 0.4355 0.5189 −24% 10378 Metabolite - 2287 35 0.1438 0.3664 346% 10438 gamma - glu-leu 35 0.4822 0.5344 −6% 10461 Metabolite - 2313 35 0.5649 0.5664 −6% 10476 Metabolite - 2316 35 0.2043 0.4036 51% 10544 Metabolite - 2329 35 1.00E−04 0.0086 −42% 10551 Metabolite - 2347 35 0.3563 0.481 30% 10604 Metabolite - 2370 35 0.8174 0.6449 4% 10629 Metabolite - 2386 35 0.516 0.5502 −14% 10644 Metabolite - 2387 35 0.5362 0.5577 −32% 10655 Metabolite - 2388 35 0.1466 0.3699 11% 10667 Metabolite - 2389 35 0.0776 0.291 186% 10672 Metabolite - 2390 35 0.0718 0.2829 −27% 10692 Metabolite - 2391 35 0.0982 0.3052 −12% 10698 Metabolite - 2392 35 0.6996 0.6131 −15% 10737 Isobar-1-includes-mannose- 35 0.8978 0.6568 −2% fructose-glucose-galactose-alpha - L-sorbopyranose-Inositol-D- allose-D--altrose-D-psicone 10739 Metabolite - 2407 35 0.0093 0.0976 48% 10741 Isobar-2-includes-2- 35 0.5412 0.5606 −15% aminoisobutyric acid-3-amino- isobutyrate-2-amino-butyrate-4- aminobutanoic acid- dimethylglycine-choline- 10743 Isobar-4-includes-Gluconic 35 0.3542 0.481 14% acid-DL-arabinose-D-ribose-L- xylose-DL-lyxose-D-xylulose 10744 Isobar-5-includes-asparagine- 35 0.1653 0.3788 17% ornithine-gly-gly 10746 Isobar-6-includes-valine-betaine 35 0.0606 0.2681 −8% 10753 Isobar-9-includes-galactinol- 35 0.7716 0.6377 −6% dihydrate-turanose-kojibiose-D- leucrose-lactulose-sophorose- sucrose-beta - D-lactose-D- trehalose-D-cellobiose-D- Maltose-palatinose-melibiose- alpha - D-lactose 10782 Metabolite - 2486 35 0.6665 0.6048 −8% 10785 Metabolite - 2506 35 0.1515 0.3712 −39% 10787 Metabolite - 2507 35 0.2156 0.4171 −34% 10825 Metabolite - 2546 35 0.4358 0.5189 −13% 11053 Metabolite - 2567 35 0.5492 0.5628 6% 11111 Metabolite - 2592 35 0.3309 0.481 −41% 11219 Metabolite - 2686 35 0.9155 0.6628 1% 11222 Metabolite - 2688 35 0.6923 0.6112 6% 11323 Metabolite - 2711 35 0.0036 0.0655 −21% 11438 phosphate 50 0.9259 0.6628 −1% 11499 Metabolite - 2753 35 0.6692 0.6048 −5% 11777 glycine 50 0.1082 0.3168 −16% 11813 Metabolite - 2809 35 0.7365 0.6215 −7% 12035 nonanate 50 0.4925 0.5413 3% 12109 Metabolite - 2853 35 0.6348 0.6006 −8% 12298 Metabolite - 2867 35 0.1809 0.3851 −47% 12444 Metabolite - 2888-possible- 35 0.3955 0.4965 13% sulfated-Rosiglitazone 12478 Metabolite - 2898 35 0.2404 0.4472 −36% 12532 Metabolite - 2914 50 0.2774 0.4695 2% 12533 Metabolite - 2915 50 0.4585 0.5239 7% 12543 2-hydroxy-butanoic acid 50 0.1732 0.3828 −17% 12562 Metabolite - 2955 50 0.8848 0.6555 1% 12593 Metabolite - 2973 50 0.8416 0.6512 1% 12594 Metabolite - 2974 50 0.1138 0.3224 11% 12601 Metabolite - 2978 50 0.0765 0.291 23% 12625 Metabolite - 3002 50 0.0539 0.2523 −21% 12626 Metabolite - 3003 50 0.9736 0.6761 0% 12627 Metabolite - 3004 50 0.9538 0.6641 −1% 12639 Metabolite - 3012 50 0.384 0.4882 7% 12641 meso-erythritol 50 0.1016 0.3119 −16% 12644 Metabolite - 3016 50 0.1504 0.3712 −9% 12645 Metabolite - 3017 50 0.2449 0.4523 15% 12647 Metabolite - 3019 50 0.232 0.4348 11% 12648 Metabolite - 3020 50 0.5697 0.5664 7% 12650 Metabolite - 3022 50 0.6591 0.6042 5% 12656 Metabolite - 3025 50 0.3225 0.481 9% 12658 Metabolite - 3026 50 0.321 0.481 10% 12663 Metabolite - 3030 50 0.0082 0.0976 22% 12666 Metabolite - 3033 50 0.0244 0.1479 14% 12673 Metabolite - 3040 50 0.0517 0.2487 27% 12682 Metabolite - 3044 35 0.5288 0.5577 −8% 12719 Metabolite - 3055 35 0.4505 0.5239 21% 12720 Metabolite - 3056 35 0.7025 0.6131 −3% 12726 Metabolite - 3058 50 0.4992 0.5413 12% 12739 1-5-anhydro-D-glucitol 50 0.3236 0.481 −12% 12751 Metabolite - 3073 50 0.1743 0.3828 23% 12753 Metabolite - 3074 50 0.136 0.3538 −39% 12754 Metabolite - 3075 50 0.6421 0.6006 6% 12756 Metabolite - 3077 50 0.5522 0.5628 6% 12757 Metabolite - 3078 50 0.4196 0.5151 9% 12761 Metabolite - 3081 50 0.4686 0.5282 −8% 12765 inositol 50 0.2758 0.4695 −12% 12768 Metabolite - 3088 50 0.0202 0.1462 38% 12769 Metabolite - 3089 50 0.2713 0.4695 −14% 12771 Metabolite - 3091 50 0.2132 0.4171 48% 12773 Metabolite - 3093 50 0.7501 0.6267 −5% 12774 Metabolite - 3094 50 0.5302 0.5577 −7% 12777 Metabolite - 3097 50 0.9998 0.6849 0% 12780 Metabolite - 3098 50 0.0769 0.291 31% 12781 Metabolite - 3099 50 0.8957 0.6568 2% 12784 Metabolite - 3102 50 0.3579 0.481 −7% 12790 Metabolite - 3108 50 0.8722 0.6522 1% 12876 Metabolite - 3125 35 0.4287 0.5189 −6% 12912 Metabolite - 3129 35 0.9973 0.6849 0% 12924 Metabolite - 3131 35 0.2901 0.4739 29% 12931 DL-hexanoyl-carnitine 35 0.7104 0.6136 −3% 12960 Metabolite - 3134 35 0.6932 0.6112 −11% 12969 Metabolite - 3135 35 0.7666 0.6364 10% 13018 Metabolite - 3138 35 0.1116 0.3215 −20% 13038 Metabolite - 3143 35 0.1583 0.3769 −17% 13065 Metabolite - 3146 35 0.0835 0.2916 −33% 13104 Metabolite - 3160 35 0.1779 0.3843 −15% 13142 Metabolite - 3165 35 0.2842 0.4702 −7% 13146 Metabolite - 3166 35 0.9041 0.6568 −3% 13148 Metabolite - 3167 35 0.9239 0.6628 1% 13179 possible-Metabolite - 3176- 35 0.1352 0.3538 28% possible-creatine 13208 Metabolite - 3181 35 0.5937 0.5841 −6% 13211 Metabolite - 3182 35 0.649 0.6015 11% 13214 Metabolite - 3183-possible- 35 0.946 0.6641 1% gamma - L-glutamyl-L- phenylalanine 13217 Metabolite - 3184 35 0.3141 0.481 −13% 13249 Metabolite - 3215 35 0.2706 0.4695 −13% 13251 Metabolite - 3216 35 0.2161 0.4171 −16% 13257 Metabolite - 3218 35 0.8158 0.6449 −4% 13342 Metabolite - 3243 35 0.9895 0.6834 0% 13448 Metabolite - 3303 35 0.0146 0.128 −17% 13459 Metabolite - 3305 35 1.00E−04 0.0086 −59% 13484 Metabolite - 3309 35 0.0639 0.2681 −27% 13505 Metabolite - 3313 35 0.3298 0.481 −38% 13509 Metabolite - 3314 35 0.1312 0.3519 −19% 13534 Metabolite - 3320-possible- 35 0.556 0.5628 −21% pimpinellin-or- tetrahydroxybenzophenone 13545 Metabolite - 3322 35 5.00E−04 0.0178 −39% 13589 Metabolite - 3327 35 5.00E−04 0.0178 −52% 13775 Metabolite - 3370 35 0.4705 0.5282 −7% 13803 Metabolite - 3377 35 0 0.0026 −64% 13904 Metabolite - 3402 35 0.0047 0.0777 −38% 14027 Metabolite - 3426 35 0.5712 0.5664 −2% 14084 Metabolite - 3436 35 0.8882 0.6558 2% 14117 Metabolite - 3441 35 0.4955 0.5413 −6% 14239 Metabolite - 3474 35 0.168 0.3788 20% 14249 Metabolite - 3476 35 0.7254 0.6178 −8% 14368 Metabolite - 3489 35 0.1059 0.3151 −35% 14439 Metabolite - 3498 35 0.4571 0.5239 9% 14495 Metabolite - 3534 35 0.1123 0.3215 −23% 14595 Metabolite - 3576 35 0.3216 0.481 19% 14608 Metabolite - 3578 35 0.3148 0.481 23% 14639 Metabolite - 3603 35 0.0022 0.0503 40% 14640 Metabolite - 3604 35 0.6656 0.6048 12% 14672 Metabolite - 3615 35 0.8695 0.6522 3% 14715 Metabolite - 3653-possible- 35 0.7021 0.6131 13% stachydrine- 14766 Metabolite - 3670 35 0.5739 0.5668 −5% 14785 isobar-glycochenodeoxycholic 35 0.6413 0.6006 −12% acid-glycodeoxycholic acid 14787 Metabolite - 3698 35 0.798 0.6385 −4% 14837 Metabolite - 3707 35 0.0965 0.3035 108% 14961 Metabolite - 3752 35 0.2823 0.4701 145% 15000 Metabolite - 3758 35 0.123 0.3334 −38% 15017 Metabolite - 3761 35 0.8844 0.6555 −2% 15032 Metabolite - 3765 35 0.6458 0.6006 −11% 15063 Metabolite - 3772 35 0.0788 0.291 −15% 15113 Metabolite - 3783 35 0.4349 0.5189 12% 15122 glycerol 50 0.0247 0.1479 −14% 15128 DL-homocysteine 35 0.3684 0.481 −11% 15129 D-alanyl-D-alanine 35 0.8683 0.6522 −2% 15211 Metabolite - 3807 35 0.2961 0.4806 −6% 15220 Metabolite - 3813 35 0.0871 0.295 −23% 15227 Metabolite - 3816 35 0.8378 0.6512 −3% 15251 Metabolite - 3830 35 0.0863 0.295 −24% 15253 Metabolite - 3832-possible- 35 0.0475 0.2374 −49% phenol-sulfate 15278 Metabolite - 3843 35 0.0309 0.1711 −17% 15319 DL-phenyllactic acid 35 0.2786 0.4695 −22% 15326 Metabolite - 3879 35 0.6363 0.6006 28% 15328 azelaic acid 35 0.1571 0.3769 −10% 15336 tartaric acid 35 0.0214 0.1462 −38% 15365 sn-Glycerol-3-phosphate 50 0.5069 0.547 −5% 15389 Metabolite - 3900 35 0.0294 0.1667 −14% 15500 carnitine 35 0.197 0.3977 10% 15529 Metabolite - 3951 35 0.6687 0.6048 −3% 15535 Metabolite - 3955 35 0.4421 0.5216 27% 15606 Metabolite - 3968 35 0.8468 0.6519 −4% 15612 Metabolite - 3972 35 0.3503 0.481 11% 15626 Metabolite - 3977 35 0.0207 0.1462 −17% 15677 3-methyl-L-histidine 35 0.3568 0.481 −6% 15681 4-Guanidinobutanoic acid 35 0.8904 0.6558 −2% 15683 4-methyl-2-oxopentanoate 50 0.1063 0.3151 −16% 15704 heptanedioic acid 35 0.1218 0.3334 47% 15744 N-N-dimethylarginine 35 0.3455 0.481 −8% 15753 hippuric acid 35 0.6581 0.6042 −9% 15991 L-alpha - 35 0.3514 0.481 −18% glycerophosphorylcholine 16002 Metabolite - 3992-possible-Cl- 35 0.643 0.6006 3% adduct-of-Formate-dimer 16016 Metabolite - 3994 35 0.7835 0.6385 −8% 16071 Metabolite - 4020 50 0.7967 0.6385 −1% 16091 Metabolite - 4031-possible- 35 0.0804 0.2916 −12% norlevorphenol- isobutylphendienamide- amprolium 16107 lysine 50 0.1771 0.3843 −12% 16137 Metabolite - 4078 35 0.2647 0.4695 −19% 16161 gamma - glutamyl-glutamic acid 35 0.9512 0.6641 0% 16186 Metabolite - 4096-possible- 35 0.4824 0.5344 19% gamma-glu-gly-leu- 16226 Isobar-28-includes-L-threonine- 35 0.1998 0.3977 14% L-allothreonine-L-homoserine- S-4-amino-2-hydroxybutyric acid 16231 Isobar-20-includes-fumaric 35 0.5237 0.5561 12% acid-3-methyl-2-oxobutanoate 16232 Isobar-17-includes-arginine-N- 35 0.0125 0.1175 36% alpha - acetyl-ornithine 16233 Isobar-13-includes-5-keto-D- 35 0.1598 0.3771 −19% gluconic acid-2-keto-L-gulonic acid-D-glucuronic acid-D- galacturonic acid 16235 Isobar-19-includes-D-saccharic 35 0.5502 0.5628 11% acid-1-5-anhydro-D-glucitol-2- deoxy-D-galactose-2-deoxy-D- glucose-L-fucose-L-rhamnose 16237 Isobar-25-includes-L-gulono-1- 35 0.7148 0.6154 −8% 4-lactone-glucono-gamma- lactone- 16241 Isobar-30-includes- 35 0.6804 0.6106 −6% maltotetraose-stachyose 16243 L-kynurenine 35 0.7838 0.6385 −3% 16244 Isobar-21-includes-gamma- 35 0.6805 0.6106 7% aminobutyryl-L-histidine-L- anserine 16246 Isobar-18-includes-D-fructose- 35 0.8566 0.6522 4% 1-phosphate-beta - D-fructose-6- phosphate 16279 Isobar-36-includes-D-sorbitol-6- 35 0.3438 0.481 −13% phosphate-mannitol-1-phosphate 16290 Metabolite - 4133 50 0.6833 0.6107 5% 16308 Metabolite - 4147 50 0.0642 0.2681 −24% 16330 Metabolite - 4163 35 0.4532 0.5239 −12% 16337 Metabolite - 4167 35 0.2792 0.4695 −17% 16462 Metabolite - 4234 35 0.4546 0.5239 −8% 16471 Metabolite - 4238 35 0.9246 0.6628 2% 16508 Metabolite - 4272 50 0.3504 0.481 −12% 16621 Metabolite - 4355 50 0.3773 0.4856 −17% 16653 Metabolite - 4361 50 0.3706 0.481 17% 16666 Metabolite - 4365 50 0.7897 0.6385 −4% 16824 iminodiacetic acid 50 0.041 0.213 −26% 16829 Metabolite - 4503 50 0.8049 0.639 −7% 16848 Metabolite - 4511 50 0.0692 0.2829 −25% 16952 Metabolite - 4593 50 0.6542 0.604 2% 17028 Metabolite - 4611 50 0.3655 0.481 −6% 17328 Metabolite - 4768 50 0.7791 0.6385 −7% 17330 Metabolite - 4769 50 0.1385 0.3566 17% 17359 Metabolite - 4791 50 0.8812 0.6555 −5% 17388 Metabolite - 4795 50 0.3033 0.481 22% 17614 Metabolite - 4966 50 0.3248 0.481 11% 17627 Metabolite - 4986 50 0.6096 0.59 12% 18118 Metabolite - 5346 50 0.6124 0.59 −6% 18146 Metabolite - 5366 50 0.6449 0.6006 7% 18232 Metabolite - 5403 50 0.2718 0.4695 10% 18316 Metabolite - 5437 50 0.4383 0.5195 −19% 18335 D-quinic acid 50 0.0891 0.295 −42% 18349 DL-indole-3-lactic acid 50 5.00E−04 0.0178 −25% 18868 Metabolite - 5847 50 0.4666 0.5282 −15% 18926 Metabolite - 5906 50 0.3425 0.481 −27% 18929 Metabolite - 5907 50 0.8229 0.6473 2%

TABLE 8 Plasma Metabolite Biomarkers to distinguish Lower Grade PCA from Higher Grade PCA. % Change in Higher COMP_ID COMPOUND LIB_ID p-value q-value PCA 53 glutamine 50 0.2081 0.4944 −13% 54 tryptophan 50 0.0734 0.3431 −13% 57 glutamic acid 50 0.3386 0.6063 −13% 59 histidine 50 0.742 0.7437 −3% 60 leucine 50 0.015 0.154 −19% 63 cholesterol 50 0.19 0.4682 −9% 64 phenylalanine 35 0.1602 0.4579 −5% 513 creatinine 35 0.9764 0.7893 0% 527 lactate 50 0.0062 0.0915 −14% 528 alpha - keto-glutarate 35 0.6988 0.7368 −9% 541 4-hydroxyphenylacetate 35 0.9884 0.7893 0% 542 3-hydroxybutanoic acid 50 0.1111 0.4081 −50% 569 caffeine 35 6.00E−04 0.0433 −70% 577 fructose 50 0.0966 0.3887 −20% 581 glucose 50 0.6171 0.7298 −2% 584 mannose 50 0.9074 0.7713 −1% 594 niacinamide 35 0.4972 0.666 −18% 597 phosphoenolpyruvate 35 0.7347 0.7434 −4% 1105 linoleic acid 50 0.0404 0.2538 −17% 1107 allantoin 50 0.7673 0.7448 −6% 1110 arachidonic acid 50 0.6643 0.7368 −6% 1121 heptadecanoic acid 50 0 0.0036 −27% 1123 inosine 35 0.972 0.7891 1% 1125 isoleucine 50 0.0129 0.1407 −19% 1126 alanine 50 0.0951 0.3887 −15% 1284 threonine 50 0.3138 0.5918 −8% 1299 tyrosine 50 0.1559 0.4579 −11% 1302 methionine 35 0.5223 0.687 −4% 1303 malic acid 35 0.5527 0.712 −13% 1336 n-hexadecanoic acid 50 0.0024 0.0649 −20% 1358 octadecanoic acid 50 9.00E−04 0.0464 −16% 1365 tetradecanoic acid 50 0.0017 0.0613 −22% 1366 trans-4-hydroxyproline 50 0.0035 0.0738 −15% 1413 3-hydroxyphenylacetate 35 0.6788 0.7368 2% 1414 3-phospho-d-glycerate 35 0.4156 0.6338 −12% 1415 4-amino-5-methyl-2-1H- 35 0.3 0.5825 −10% pyrimidinone 1431 (p-Hydroxyphenyl)lactic 50 0.0035 0.0738 −27% acid 1432 alphahydroxybenzeneacetic 35 0.8113 0.751 3% acid 1437 succinate 50 0.2184 0.5028 9% 1444 DL-pipecolic acid 35 0.9642 0.7862 0% 1480 guanidineacetic acid 35 0.8726 0.7585 −2% 1493 ornithine 50 0.1811 0.4682 −19% 1494 5-oxoproline 50 0.0231 0.2124 −12% 1498 N-6-trimethyl-l-lysine 35 0.7026 0.7368 5% 1507 palmitoleic acid 50 7.00E−04 0.0433 −44% 1508 pantothenic acid 35 0.182 0.4682 −31% 1519 sucrose 50 0.9548 0.7862 −2% 1557 3-methylglutaric acid 35 0.19 0.4682 −10% 1561 alpha - tocopherol 50 0.8757 0.759 −6% 1564 citric acid 50 0.2758 0.5606 −6% 1570 oleic acid 50 0.0045 0.0866 −34% 1572 glyceric acid 50 0.2114 0.4945 17% 1574 histamine 35 0.8416 0.7585 −4% 1584 methyl-indole-3-acetate 35 0.2877 0.569 −13% 1587 N-acetyl-L-leucine 35 0.2729 0.5593 −31% 1591 N-acetyl-L-valine 35 0.9102 0.7713 1% 1604 uric acid 35 0.813 0.751 −1% 1643 fumaric acid 50 0.5872 0.7241 −6% 1645 n-dodecanoate 50 0.1214 0.4111 −15% 1648 serine 50 0.0574 0.3133 −14% 1649 valine 50 0.0074 0.1041 −19% 1670 urea 50 0.6012 0.7257 3% 1708 7-8-dihydrofolic acid 35 0.5075 0.673 −20% 1898 proline 50 0.221 0.5048 −16% 2078 pyrophosphate 35 0.7583 0.7448 −7% 2092 catechol 35 0.2733 0.5593 −31% 2132 citrulline 50 0.3254 0.5955 9% 2730 gamma-L-glutamyl-L- 35 0.4725 0.6556 16% glutamine 2734 gamma-L-glutamyl-L- 35 0.7177 0.7423 −2% tyrosine 2832 adenosine-5-monophosphate 35 0.3158 0.5918 −14% 2848 guanosine-5-diphosphate 35 0.445 0.6491 −9% 3127 hypoxanthine 35 0.948 0.7855 1% 3138 pyridoxamine-phosphate 35 0.4558 0.6552 −12% 3147 xanthine 35 0.6472 0.73 13% 4966 xylitol 35 0.463 0.6556 13% 5280 biliverdin 35 0.1482 0.4503 −33% 5331 pyridoxal-phosphate 35 0.0379 0.2538 9% 5618 Metabolite - 1085-possible- 35 0.0709 0.3425 16% isolobinine-or-4-aminoestra- 1-3-5-10-triene-3-17beta- diol 5628 Metabolite - 1086 35 0.6001 0.7257 −8% 5669 Metabolite - 1104 35 0.0196 0.1865 −14% 5687 Metabolite - 1110 35 0.3645 0.6113 37% 5689 Metabolite - 1111-possible- 35 0.4217 0.6338 −9% methylnitronitrosoguanidine- or-ethyl- thiocarbamoylacetate 5697 acetylcarnitine- 35 0.262 0.559 −11% 5717 Metabolite - 1121 35 0.1136 0.4081 17% 5733 Metabolite - 1127 35 0.0695 0.3413 −20% 5765 Metabolite - 1142-possible- 35 0.5859 0.7241 −18% 5-hydroxypentanoate-or- beta-hydroxyisovaleric acid 5788 Metabolite - 1183 35 0.3872 0.6303 −55% 5792 Metabolite - 1185 35 0.0919 0.3869 24% 5800 Metabolite - 1188 35 0.4258 0.6338 −13% 6112 Metabolite - 1203-HXGXA 35 0.1905 0.4682 −48% in-MTRX 6130 Metabolite - 1208 35 0.3544 0.6113 −20% 6136 Metabolite - 1211-possible- 35 0.6994 0.7368 −22% IHWESASLLR- 6144 Metabolite - 1215 35 0.7751 0.7448 10% 6147 Metabolite - 1216 35 0.3347 0.6063 −15% 6155 Metabolite - 1220 35 0.9929 0.7893 0% 6171 Metabolite - 1244 35 0.3205 0.5918 −15% 6266 Metabolite - 1286 35 0.1856 0.4682 11% 6278 Metabolite - 1289 35 0.4585 0.6556 14% 6362 Metabolite - 1323-possible- 35 0.9961 0.7893 0% p-cresol-sulfate 6398 Metabolite - 1335 35 0.8918 0.766 2% 6413 Metabolite - 1342-possible- 35 0.7003 0.7368 −8% phenylacetylglutamine-or- formyl-N-acetyl-5- methoxykynurenamine 6437 Metabolite - 1349-possible- 35 0.9819 0.7893 −1% N-acetyl-8-O-methyl- Neuraminic acid 6443 Metabolite - 1351 35 0.871 0.7585 −2% 6537 Metabolite - 1389-possible- 35 0.302 0.5825 −78% gemfibrozil-glucuronide- 6549 Metabolite - 1392 35 0.4157 0.6338 −15% 6787 Metabolite - 1465 35 0.649 0.73 −8% 6852 Metabolite - 1498 35 0.0777 0.3524 33% 6987 Metabolite - 1573 35 0.9489 0.7855 −1% 7029 Metabolite - 1597 35 0.4038 0.6338 −6% 7081 Metabolite - 1609 35 0.0305 0.2368 −23% 7177 Metabolite - 1656 35 0.4157 0.6338 13% 7359 n-acetyl-L-aspartic acid 35 0.0437 0.2628 −21% 7446 p-hydroxybenzaldehyde 35 0.6766 0.7368 −4% 7595 Metabolite - 1817 35 0.153 0.4554 21% 7639 oxalic acid 35 0.2078 0.4944 11% 7644 Metabolite - 1831-possible- 35 0.0691 0.3413 −44% Cl-adduct-of-citrulline 7650 Metabolite - 1834 35 0.1156 0.4081 −30% 7652 Metabolite - 1835 35 0.4281 0.6338 −11% 7654 Metabolite - 1836 35 0.147 0.4503 −25% 7660 Metabolite - 1839 35 0.0387 0.2538 −42% 7672 Metabolite - 1843 35 0.7808 0.7448 −3% 7933 Metabolite - 1911 35 0.5335 0.6956 −16% 7935 paraxanthine 35 0.0019 0.0613 −58% 7941 Metabolite - 1914 35 0.4757 0.6556 25% 7944 Metabolite - 1915 35 0.6141 0.7297 −26% 7957 trans-2-3-4- 35 0.4257 0.6338 28% trimethoxycinnamic acid 8091 glycocholic acid 35 0.4181 0.6338 −27% 8176 Metabolite - 1974 35 0.7599 0.7448 −5% 8189 Metabolite - 1977 35 0.0864 0.3689 −14% 8196 Metabolite - 1979-Cl- 35 0.6427 0.73 6% adduct-of-isobar-19 8217 Metabolite - 1983 35 0.6235 0.73 −32% 8300 Metabolite - 1988 35 0.0635 0.3225 −19% 8336 Metabolite - 2005 35 0.0395 0.2538 −25% 8404 Metabolite - 2027 35 0.3196 0.5918 −10% 8649 Metabolite - 2053 35 0.1695 0.4624 −19% 8669 Metabolite - 2055 35 0.9053 0.7713 3% 8677 Metabolite - 2056 35 0.0608 0.3144 −20% 8796 Metabolite - 2074 35 0.7798 0.7448 4% 8959 Metabolite - 2100 35 0.9464 0.7855 −1% 9007 Metabolite - 2108 35 0.0164 0.1608 −29% 9024 Metabolite - 2111 35 0.5873 0.7241 −10% 9092 Metabolite - 2129 35 0.8698 0.7585 5% 9106 Metabolite - 2130 35 0.0606 0.3144 95% 9130 Metabolite - 2139 35 0.6874 0.7368 5% 9137 Metabolite - 2141 35 0.0058 0.0915 −49% 9491 Metabolite - 2185 35 0.1596 0.4579 −12% 9748 Metabolite - 2212 35 0.0811 0.355 31% 10087 Metabolite - 2249 35 0.8331 0.7585 −3% 10092 Metabolite - 2250 35 0.2327 0.5235 −27% 10122 Metabolite - 2254 35 0.3765 0.6227 −29% 10143 Metabolite - 2255- 35 0.6393 0.73 27% hydroxyproline-form-of- bradykinin 10145 Metabolite - 2256 35 0.4476 0.6495 18% 10245 Metabolite - 2269- 35 0.4123 0.6338 24% 10317 Metabolite - 2279 35 0.0505 0.2916 −42% 10327 Metabolite - 2281 35 0.3395 0.6063 −44% 10378 Metabolite - 2287 35 0.2325 0.5235 180% 10438 gamma-glu-leu 35 0.245 0.5351 −10% 10461 Metabolite - 2313 35 0.7899 0.7448 −3% 10476 Metabolite - 2316 35 0.1189 0.4081 74% 10544 Metabolite - 2329 35 0 0.0014 −42% 10551 Metabolite - 2347 35 0.5251 0.6877 20% 10604 Metabolite - 2370 35 0.9852 0.7893 0% 10629 Metabolite - 2386 35 0.5977 0.7257 −11% 10644 Metabolite - 2387 35 0.3024 0.5825 −55% 10655 Metabolite - 2388 35 0.1144 0.4081 12% 10667 Metabolite - 2389 35 0.1156 0.4081 138% 10672 Metabolite - 2390 35 0.0817 0.355 −41% 10692 Metabolite - 2391 35 0.3467 0.6113 −7% 10698 Metabolite - 2392 35 0.8622 0.7585 −7% 10737 Isobar-1-includes-mannose- 35 0.6822 0.7368 −6% fructose-glucose-galactose- alpha-L-sorbopyranose- Inositol-D-allose-D--altrose- D-psicone 10739 Metabolite - 2407 35 0.0119 0.1402 48% 10741 Isobar-2-includes-2- 35 0.6689 0.7368 −7% aminoisobutyric acid-3- amino-isobutyrate-2-amino- butyrate-4-aminobutanoic acid-dimethylglycine- choline- 10743 Isobar-4-includes-Gluconic 35 0.3076 0.5847 16% acid-DL-arabinose-D- ribose-L-xylose-DL-lyxose- D-xylulose 10744 Isobar-5-includes- 35 0.2642 0.559 13% asparagine-ornithine-gly-gly 10746 Isobar-6-includes-valine- 35 0.0032 0.0738 −12% betaine 10753 Isobar-9-includes- 35 0.9571 0.7862 1% galactinol-dihydrate- turanose-kojibiose-D- leucrose-lactulose- sophorose-sucrose-beta-D- lactose-D-trehalose-D- cellobiose-D-Maltose- palatinose-melibiose-alpha- D-lactose 10782 Metabolite - 2486 35 0.427 0.6338 −15% 10785 Metabolite - 2506 35 0.1333 0.4318 −41% 10787 Metabolite - 2507 35 0.354 0.6113 −25% 10825 Metabolite - 2546 35 0.4672 0.6556 −13% 11053 Metabolite - 2567 35 0.7367 0.7434 −3% 11111 Metabolite - 2592 35 0.1638 0.4579 −62% 11219 Metabolite - 2686 35 0.7815 0.7448 −2% 11222 Metabolite - 2688 35 0.6332 0.73 −7% 11323 Metabolite - 2711 35 0.0047 0.0866 −21% 11438 phosphate 50 0.7922 0.7448 −1% 11499 Metabolite - 2753 35 0.3071 0.5847 −11% 11777 glycine 50 0.355 0.6113 −9% 11813 Metabolite - 2809 35 0.5982 0.7257 −10% 12035 nonanate 50 0.6366 0.73 2% 12109 Metabolite - 2853 35 0.0251 0.2202 −34% 12298 Metabolite - 2867 35 0.9249 0.7787 −4% 12478 Metabolite - 2898 35 0.4273 0.6338 30% 12532 Metabolite - 2914 50 0.8816 0.7596 0% 12533 Metabolite - 2915 50 0.1622 0.4579 14% 12543 2-hydroxy-butanoic acid 50 0.0286 0.2334 −29% 12562 Metabolite - 2955 50 0.5565 0.7129 −2% 12593 Metabolite - 2973 50 0.8623 0.7585 1% 12594 Metabolite - 2974 50 0.1248 0.4179 11% 12601 Metabolite - 2978 50 0.1299 0.4252 19% 12625 Metabolite - 3002 50 0.0819 0.355 −20% 12626 Metabolite - 3003 50 0.4436 0.6491 9% 12627 Metabolite - 3004 50 0.6919 0.7368 5% 12639 Metabolite - 3012 50 0.8549 0.7585 2% 12641 meso-erythritol 50 0.163 0.4579 −9% 12644 Metabolite - 3016 50 0.6845 0.7368 −3% 12645 Metabolite - 3017 50 0.7813 0.7448 −3% 12647 Metabolite - 3019 50 0.6788 0.7368 4% 12648 Metabolite - 3020 50 0.9322 0.7826 1% 12650 Metabolite - 3022 50 0.6859 0.7368 5% 12656 Metabolite - 3025 50 0.9228 0.7787 1% 12658 Metabolite - 3026 50 0.7257 0.7423 3% 12663 Metabolite - 3030 50 0.114 0.4081 12% 12666 Metabolite - 3033 50 0.0152 0.154 15% 12673 Metabolite - 3040 50 0.2695 0.5593 15% 12682 Metabolite - 3044 35 0.1443 0.4503 −18% 12719 Metabolite - 3055 35 0.5731 0.7241 15% 12720 Metabolite - 3056 35 0.9358 0.7834 1% 12726 Metabolite - 3058 50 0.6191 0.7298 9% 12739 1-5-anhydro-D-glucitol 50 0.7986 0.7448 −4% 12751 Metabolite - 3073 50 0.1953 0.4717 22% 12753 Metabolite - 3074 50 0.3904 0.6321 −22% 12754 Metabolite - 3075 50 0.2559 0.5505 15% 12756 Metabolite - 3077 50 0.5804 0.7241 5% 12757 Metabolite - 3078 50 0.2799 0.5648 11% 12761 Metabolite - 3081 50 0.8489 0.7585 1% 12765 inositol 50 0.5387 0.6992 −7% 12768 Metabolite - 3088 50 0.0017 0.0613 63% 12769 Metabolite - 3089 50 0.0293 0.2334 −26% 12771 Metabolite - 3091 50 0.4809 0.6576 22% 12773 Metabolite - 3093 50 0.4029 0.6338 −13% 12774 Metabolite - 3094 50 0.4972 0.666 −7% 12777 Metabolite - 3097 50 0.3855 0.6303 −14% 12780 Metabolite - 3098 50 0.2867 0.569 17% 12781 Metabolite - 3099 50 0.1762 0.4679 −19% 12784 Metabolite - 3102 50 0.3989 0.6338 −6% 12790 Metabolite - 3108 50 0.8644 0.7585 −1% 12876 Metabolite - 3125 35 0.7646 0.7448 −2% 12912 Metabolite - 3129 35 0.4822 0.6576 −8% 12924 Metabolite - 3131 35 0.3961 0.6338 23% 12931 DL-hexanoyl-carnitine 35 0.487 0.6583 −6% 12960 Metabolite - 3134 35 0.6922 0.7368 −11% 12969 Metabolite - 3135 35 0.9108 0.7713 −3% 13018 Metabolite - 3138 35 0.7404 0.7437 −4% 13038 Metabolite - 3143 35 0.1276 0.4226 −18% 13065 Metabolite - 3146 35 0.0976 0.3887 −35% 13104 Metabolite - 3160 35 0.6297 0.73 −6% 13142 Metabolite - 3165 35 0.7536 0.7448 −2% 13146 Metabolite - 3166 35 0.7532 0.7448 −7% 13148 Metabolite - 3167 35 0.217 0.5028 −16% 13179 possible-Metabolite - 3176- 35 0.1906 0.4682 22% possible-creatine 13208 Metabolite - 3181 35 0.7725 0.7448 3% 13211 Metabolite - 3182 35 0.6544 0.7332 11% 13214 Metabolite - 3183-possible- 35 0.8943 0.766 −1% gamma-L-glutamyl-L- phenylalanine 13217 Metabolite - 3184 35 0.8706 0.7585 −2% 13249 Metabolite - 3215 35 0.4668 0.6556 −9% 13251 Metabolite - 3216 35 0.7987 0.7448 −4% 13257 Metabolite - 3218 35 0.5009 0.6678 −8% 13342 Metabolite - 3243 35 0.6378 0.73 −9% 13448 Metabolite - 3303 35 0.2433 0.5351 −9% 13459 Metabolite - 3305 35 0.0084 0.1083 −50% 13484 Metabolite - 3309 35 0.8307 0.7585 −4% 13505 Metabolite - 3313 35 0.3577 0.6113 −20% 13509 Metabolite - 3314 35 0.3672 0.6113 −12% 13534 Metabolite - 3320-possible- 35 0.4843 0.6576 −28% pimpinellin-or- tetrahydroxybenzophenone 13545 Metabolite - 3322 35 0 0.0014 −47% 13589 Metabolite - 3327 35 0.0057 0.0915 −50% 13775 Metabolite - 3370 35 0.3669 0.6113 −8% 13803 Metabolite - 3377 35 0.006 0.0915 −57% 13904 Metabolite - 3402 35 0.0721 0.3428 −26% 14027 Metabolite - 3426 35 0.7318 0.7434 1% 14084 Metabolite - 3436 35 0.6058 0.7257 −10% 14117 Metabolite - 3441 35 0.7013 0.7368 5% 14239 Metabolite - 3474 35 0.0599 0.3144 31% 14249 Metabolite - 3476 35 0.7249 0.7423 9% 14368 Metabolite - 3489 35 0.3626 0.6113 −20% 14439 Metabolite - 3498 35 0.5688 0.7241 6% 14495 Metabolite - 3534 35 0.0262 0.2202 −32% 14595 Metabolite - 3576 35 0.2107 0.4945 26% 14608 Metabolite - 3578 35 0.6691 0.7368 9% 14639 Metabolite - 3603 35 0.1163 0.4081 18% 14640 Metabolite - 3604 35 0.5838 0.7241 −13% 14672 Metabolite - 3615 35 0.6102 0.728 −10% 14715 Metabolite - 3653-possible- 35 0.473 0.6556 28% stachydrine- 14766 Metabolite - 3670 35 0.147 0.4503 −13% 14785 isobar- 35 0.3213 0.5918 −25% glycochenodeoxycholic acid-glycodeoxycholic acid 14787 Metabolite - 3698 35 0.7067 0.7385 8% 14837 Metabolite - 3707 35 0.1711 0.4624 76% 14961 Metabolite - 3752 35 0.639 0.73 −25% 15000 Metabolite - 3758 35 0.1668 0.4593 −19% 15017 Metabolite - 3761 35 0.8791 0.7596 3% 15032 Metabolite - 3765 35 0.0335 0.2531 −38% 15063 Metabolite - 3772 35 0.1466 0.4503 −11% 15113 Metabolite - 3783 35 0.4353 0.6413 12% 15122 glycerol 50 0.056 0.3133 −13% 15128 DL-homocysteine 35 0.728 0.7423 3% 15129 D-alanyl-D-alanine 35 0.0754 0.3472 −21% 15211 Metabolite - 3807 35 0.8721 0.7585 −1% 15220 Metabolite - 3813 35 0.1744 0.4672 −20% 15227 Metabolite - 3816 35 0.8542 0.7585 −3% 15251 Metabolite - 3830 35 0.0126 0.1407 −36% 15253 Metabolite - 3832-possible- 35 0.7773 0.7448 −8% phenol-sulfate 15278 Metabolite - 3843 35 0.8645 0.7585 −1% 15319 DL-phenyllactic acid 35 0.0478 0.2816 −41% 15326 Metabolite - 3879 35 0.7977 0.7448 13% 15328 azelaic acid 35 0.2378 0.5309 −9% 15336 tartaric acid 35 0.0388 0.2538 −38% 15365 sn-Glycerol-3-phosphate 50 0.2692 0.5593 −8% 15389 Metabolite - 3900 35 0.0079 0.1061 −16% 15500 carnitine 35 0.344 0.6106 7% 15529 Metabolite - 3951 35 0.2451 0.5351 9% 15535 Metabolite - 3955 35 0.3382 0.6063 37% 15606 Metabolite - 3968 35 0.9659 0.7862 1% 15612 Metabolite - 3972 35 0.7724 0.7448 2% 15626 Metabolite - 3977 35 0.1527 0.4554 −10% 15677 3-methyl-L-histidine 35 0.5533 0.712 3% 15681 4-Guanidinobutanoic acid 35 0.3014 0.5825 15% 15683 4-methyl-2-oxopentanoate 50 0.0405 0.2538 −20% 15704 heptanedioic acid 35 0.1181 0.4081 51% 15744 N—N-dimethylarginine 35 0.1838 0.4682 −11% 15753 hippuric acid 35 0.3545 0.6113 −17% 15991 L-alpha- 35 0.4761 0.6556 −16% glycerophosphorylcholine 16002 Metabolite - 3992-possible- 35 0.586 0.7241 4% Cl-adduct-of-Formate-dimer 16016 Metabolite - 3994 35 0.9895 0.7893 0% 16071 Metabolite - 4020 50 0.3655 0.6113 −6% 16091 Metabolite - 4031-possible- 35 0.1019 0.4004 −11% norlevorphenol- isobutylphendienamide- amprolium 16107 lysine 50 0.1647 0.4579 −14% 16137 Metabolite - 4078 35 0.7185 0.7423 −8% 16161 gamma-glutamyl-glutamic 35 0.5784 0.7241 −8% acid 16186 Metabolite - 4096-possible- 35 0.9805 0.7893 1% gamma-glu-gly-leu- 16226 Isobar-28-includes-L- 35 0.2869 0.569 11% threonine-L-allothreonine- L-homoserine-S-4-amino-2- hydroxybutyric acid 16231 Isobar-20-includes-fumaric 35 0.7914 0.7448 −5% acid-3-methyl-2- oxobutanoate 16232 Isobar-17-includes-arginine- 35 0.01 0.1225 38% N-alpha-acetyl-ornithine 16233 Isobar-13-includes-5-keto- 35 0.1191 0.4081 −21% D-gluconic acid-2-keto-L- gulonic acid-D-glucuronic acid-D-galacturonic acid 16235 Isobar-19-includes-D- 35 0.6445 0.73 8% saccharic acid-1-5-anhydro- D-glucitol-2-deoxy-D- galactose-2-deoxy-D- glucose-L-fucose-L- rhamnose 16237 Isobar-25-includes-L- 35 0.821 0.756 −5% gulono-1-4-lactone-glucono- gamma-lactone- 16241 Isobar-30-includes- 35 0.2512 0.5443 −15% maltotetraose-stachyose 16243 L-kynurenine 35 0.6009 0.7257 −5% 16244 Isobar-21-includes-gamma- 35 0.1351 0.4326 36% aminobutyryl-L-histidine-L- anserine 16246 Isobar-18-includes-D- 35 0.9657 0.7862 −1% fructose-1-phosphate-beta- D-fructose-6-phosphate 16279 Isobar-36-includes-D- 35 0.0397 0.2538 −26% sorbitol-6-phosphate- mannitol-1-phosphate 16290 Metabolite - 4133 50 0.1945 0.4717 16% 16308 Metabolite - 4147 50 0.1066 0.4081 −21% 16330 Metabolite - 4163 35 0.866 0.7585 −3% 16337 Metabolite - 4167 35 0.4046 0.6338 −14% 16462 Metabolite - 4234 35 0.7831 0.7448 −4% 16471 Metabolite - 4238 35 0.7117 0.741 −8% 16508 Metabolite - 4272 50 0.939 0.7839 −1% 16621 Metabolite - 4355 50 0.9609 0.7862 −1% 16653 Metabolite - 4361 50 0.8571 0.7585 −3% 16666 Metabolite - 4365 50 0.6891 0.7368 7% 16824 iminodiacetic acid 50 0.0261 0.2202 −23% 16829 Metabolite - 4503 50 0.839 0.7585 −7% 16848 Metabolite - 4511 50 0.0959 0.3887 −23% 16952 Metabolite - 4593 50 0.7253 0.7423 −2% 17028 Metabolite - 4611 50 0.7939 0.7448 −2% 17328 Metabolite - 4768 50 0.414 0.6338 −17% 17330 Metabolite - 4769 50 0.8061 0.7493 3% 17359 Metabolite - 4791 50 0.9964 0.7893 0% 17388 Metabolite - 4795 50 0.6053 0.7257 10% 17614 Metabolite - 4966 50 0.866 0.7585 2% 17627 Metabolite - 4986 50 0.4496 0.6495 −13% 18118 Metabolite - 5346 50 0.2656 0.559 −11% 18146 Metabolite - 5366 50 0.0574 0.3133 −23% 18232 Metabolite - 5403 50 0.6472 0.73 4% 18316 Metabolite - 5437 50 0.0382 0.2538 −38% 18335 D-quinic acid 50 0.0437 0.2628 −48% 18349 DL-indole-3-lactic acid 50 0.0021 0.0633 −22% 18868 Metabolite - 5847 50 0.3783 0.6227 −18% 18926 Metabolite - 5906 50 0.4724 0.6556 −29% 18929 Metabolite - 5907 50 0.5093 0.673 −6%

Example 4 Distinguish Lower Grade from Higher Grade, Urine

Biomarkers were discovered by (1) analyzing urine samples from different groups of human subjects to determine the levels of metabolites in the samples and then (2) statistically analyzing the results to determine those metabolites that were differentially present in the two groups.

The urine samples used for the analysis were from 53 control individuals with negative biopsies for prostate cancer, 43 individuals with lower grade prostate cancer (i.e. Gleason Score major=3) and 15 individuals with aggressive, high grade prostate cancer (i.e. Gleason Score major=4+). After the levels of metabolites were determined, the data was analyzed using univariate T-tests (i.e., Welch's T-test).

T-tests were used to determine differences in the mean levels of metabolites between two populations (i.e., Lower Grade Prostate cancer vs. Control, Metastatic/High Grade Prostate cancer vs. Control, Metastatic/High Grade Prostate cancer vs. Lower Grade Prostate cancer).

Biomarkers:

As listed below in Table 9, biomarkers were discovered that were differentially present between urine samples from subjects with lower grade prostate cancer and urine samples from Control subjects with negative prostate biopsies (i.e. not diagnosed with prostate cancer). Table 10 lists biomarkers that were discovered that were differentially present between urine samples from subjects with metastatic/high grade prostate cancer and urine samples from Control subjects with biopsy negative prostates (i.e. not diagnosed with prostate cancer). Table 11 lists biomarkers that were discovered that were differentially present between urine samples from subjects with metastatic/high grade prostate cancer and urine from subjects with lower grade prostate cancer.

Tables 9-11 include, for each listed biomarker, the p-value and q-value determined in the statistical analysis of the data concerning the biomarkers and an indication of the percentage difference in the lower grade prostate cancer mean level as compared to the control mean level (Table 9), the metastatic/high grade prostate cancer mean level as compared to the control mean level (Table 10), and the metastatic/high grade prostate cancer mean level as compared to the lower grade prostate cancer mean level (Table 11). The term “Isobar” as used in the tables indicates the compounds that could not be distinguished from each other on the analytical platform used in the analysis (i.e., the compounds in an isobar elute at nearly the same time and have similar (and sometimes exactly the same) quant ions, and thus cannot be distinguished). Library indicates the chemical library that was used to identify the compounds. The number 50 refers to the GC library and the number 35 refers to the LC library.

Biomarkers were discovered by (1) analyzing urine samples from different groups of human subjects to determine the levels of metabolites in the samples and then (2) statistically analyzing the results to determine those metabolites that were differentially present in the two groups.

The urine samples used for the analysis were from 53 control individuals with negative biopsies for prostate cancer, 43 individuals with lower grade prostate cancer (i.e. Gleason Score major=3) and 15 individuals with aggressive, high grade prostate cancer (i.e. Gleason Score major=4+). After the levels of metabolites were determined, the data was analyzed using univariate T-tests (i.e., Welch's T-test).

T-tests were used to determine differences in the mean levels of metabolites between two populations (i.e., Lower Grade Prostate cancer vs. Control, Metastatic/High Grade Prostate cancer vs. Control, Metastatic/High Grade Prostate cancer vs. Lower Grade Prostate cancer).

TABLE 9 Urine Metabolite Biomarkers to distinguish Non-cancer vs. Lower Grade PCA % Change COMP_ID COMPOUND LIB_ID p-value q-value in PCA 53 glutamine 50 0.796 0.9846 5% 54 tryptophan 35 0.1502 0.9846 −15% 57 glutamic acid 50 0.855 0.9846 −3% 59 histidine 50 0.4545 0.9846 17% 60 leucine 50 0.7145 0.9846 8% 64 phenylalanine 35 0.6419 0.9846 −6% 418 guanine 50 0.9595 0.9875 1% 512 asparagine 50 0.4606 0.9846 −9% 513 creatinine 35 0.1826 0.9846 −10% 521 homogentisate 50 0.8571 0.9846 −5% 527 lactate 50 0.3716 0.9846 −9% 528 alpha-keto-glutarate 35 0.1009 0.9846 35% 531 3-hydroxy-3- 50 0.9687 0.9875 0% methylglutarate 541 4-hydroxyphenylacetate 50 0.4362 0.9846 25% 542 3-hydroxybutanoic acid 50 0.6851 0.9846 27% 554 adenine 50 0.2417 0.9846 23% 555 adenosine 35 0.9098 0.9875 2% 563 alpha-L-sorbopyranose 50 0.9777 0.9875 −1% 569 caffeine 35 0.4377 0.9846 21% 575 arabinose 50 0.5366 0.9846 10% 577 fructose 50 0.4858 0.9846 31% 581 glucose 50 0.3339 0.9846 −77% 587 gluconic acid 50 0.5172 0.9846 14% 594 niacinamide 35 0.8901 0.9875 1% 597 phosphoenolpyruvate 35 0.4537 0.9846 −20% 605 uracil 50 0.4138 0.9846 15% 607 urocanic acid 35 0.2858 0.9846 39% 608 vitamin-B6 35 0.1525 0.9846 112% 1101 3-methoxy-4- 50 0.9705 0.9875 1% hydroxyphenylacetate 1107 allantoin 50 0.6965 0.9846 6% 1125 isoleucine 50 0.4588 0.9846 −12% 1126 alanine 50 0.9256 0.9875 1% 1284 threonine 50 0.7919 0.9846 5% 1299 tyrosine 50 0.8374 0.9846 4% 1302 methionine 35 0.6757 0.9846 −6% 1303 malic acid 35 0.81 0.9846 6% 1366 trans-4-hydroxyproline 50 0.2618 0.9846 22% 1413 3-hydroxyphenylacetate 35 0.8047 0.9846 −4% 1417 kynurenic acid 50 0.9471 0.9875 −1% 1418 5-6-Dihydrothymine 35 0.9664 0.9875 −1% 1419 5-s-methyl-5-thioadenosine 35 0.2486 0.9846 −15% 1431 (p-Hydroxyphenyl)lactic 50 0.1211 0.9846 32% acid 1432 alphahydroxybenzeneacetic 35 0.8358 0.9846 −2% acid 1437 succinate 50 0.0633 0.9846 42% 1444 DL-pipecolic acid 35 0.9345 0.9875 −4% 1480 guanidineacetic acid 35 0.0371 0.9846 42% 1493 ornithine 50 0.2975 0.9846 54% 1494 5-oxoproline 50 0.4228 0.9846 14% 1498 N-6-trimethyl-l-lysine 35 0.2734 0.9846 18% 1505 orotic acid 50 0.2172 0.9846 26% 1508 pantothenic acid 35 0.9729 0.9875 −1% 1519 sucrose 50 0.3449 0.9846 95% 1557 3-methylglutaric acid 35 0.8338 0.9846 3% 1558 4-acetamidobutyric acid 35 0.3994 0.9846 −13% 1559 5-6-dihydrouracil 50 0.2764 0.9846 14% 1560 L-methyldopa 35 0.3931 0.9846 19% 1564 citric acid 50 0.4898 0.9846 13% 1566 3-amino-isobutyrate 50 0.3393 0.9846 41% 1567 4-hydroxy-3- 50 0.8926 0.9875 −3% methoxymandelate 1568 4-hydroxymandelate 50 0.6452 0.9846 11% 1569 DL-beta- 35 0.4562 0.9846 16% hydroxyphenylethylamine 1574 histamine 35 0.892 0.9875 −3% 1580 noradrenaline 50 0.6485 0.9846 11% 1585 N-acetyl-L-alanine 35 0.5936 0.9846 −8% 1587 N-acetyl-L-leucine 35 0.7574 0.9846 5% 1591 N-acetyl-L-valine 35 0.2762 0.9846 34% 1592 N-acetylneuraminic acid 50 0.9603 0.9875 −1% 1598 N-tigloylglycine 35 0.434 0.9846 13% 1604 uric acid 35 0.8166 0.9846 1% 1640 ascorbic acid 50 0.5353 0.9846 113% 1648 serine 50 0.448 0.9846 10% 1649 valine 50 0.8355 0.9846 4% 1708 7-8-dihydrofolic acid 35 0.3057 0.9846 10% 1778 gamma-glu-cys 35 0.9805 0.9875 −1% 1827 riboflavine 35 0.3791 0.9846 29% 1860 3-nitro-L-tyrosine 35 0.3596 0.9846 26% 1868 cysteine 50 0.3622 0.9846 69% 1898 proline 50 0.806 0.9846 −5% 1899 quinolinic acid 50 0.8475 0.9846 3% 2078 pyrophosphate 50 0.3881 0.9846 44% 2092 catechol 35 0.9299 0.9875 3% 2132 citrulline 50 0.1077 0.9846 29% 2183 thymidine 35 0.6528 0.9846 −7% 2245 Metabolite - 294 35 0.1857 0.9846 32% 2342 serotonin 35 0.631 0.9846 6% 2734 gamma-L-glutamyl-L- 35 0.1668 0.9846 −24% tyrosine 2829 N-formyl-L-methionine 35 0.3164 0.9846 19% 2831 adenosine-3-5-cyclic- 35 0.4814 0.9846 −8% monophosphate 3127 hypoxanthine 35 0.8698 0.9875 3% 3138 pyridoxamine-phosphate 35 0.3482 0.9846 −9% 3147 xanthine 35 0.3736 0.9846 10% 3155 3-ureidopropionic acid 35 0.3809 0.9846 17% 4966 xylitol 50 0.3043 0.9846 38% 5493 Metabolite - 1059 35 0.7607 0.9846 4% 5495 Metabolite - 1060 35 0.0016 0.6911 −17% 5514 Metabolite - 1081 35 0.904 0.9875 0% 5538 Metabolite - 1101 35 0.5358 0.9846 −21% 5664 Metabolite - 1101 35 0.506 0.9846 25% 5687 Metabolite - 1110 35 0.7134 0.9846 −6% 5697 acetylcarnitine- 35 0.6634 0.9846 12% 5702 Metabolite - 1114 35 0.4207 0.9846 −23% 5711 2-hydroxybutyric acid 35 0.7116 0.9846 7% 5719 Metabolite - 1122 35 0.5481 0.9846 11% 5727 Metabolite - 1126 35 0.541 0.9846 10% 5797 Metabolite - 1186 35 0.3318 0.9846 −14% 6137 Metabolite - 1212 35 0.2597 0.9846 −50% 6147 Metabolite - 1216 35 0.5806 0.9846 4% 6238 normetanephrine 50 0.8045 0.9846 −3% 6253 Metabolite - 1283 35 0.0674 0.9846 −25% 6278 Metabolite - 1289 35 0.7974 0.9846 4% 6329 urea 50 0.903 0.9875 2% 6362 Metabolite - 1323-possible- 35 0.4176 0.9846 18% p-cresol-sulfate 6398 Metabolite - 1335 35 0.4128 0.9846 12% 6405 Metabolite - 1338 35 0.741 0.9846 −6% 6413 Metabolite - 1342-possible- 35 0.4637 0.9846 10% phenylacetylglutamine-or- formyl-N-acetyl-5- methoxykynurenamine 6421 Metabolite - 1345 35 0.5336 0.9846 20% 6437 Metabolite - 1349-possible- 35 0.5691 0.9846 10% N-acetyl-8-O-methyl- Neuraminic acid 6443 Metabolite - 1351 35 0.3894 0.9846 23% 6477 Metabolite - 1364 35 0.3757 0.9846 167% 6486 Metabolite - 1368 35 0.5104 0.9846 −22% 6493 salicyluric acid 35 0.2953 0.9846 888% 6528 Metabolite - 1383-possible- 35 0.2172 0.9846 287% salicyluric-glucuronide 6760 Metabolite - 1455 35 0.0407 0.9846 −22% 6764 Metabolite - 1459 35 0.1583 0.9846 36% 6777 Metabolite - 1463 35 0.172 0.9846 161% 6787 Metabolite - 1465 35 0.848 0.9846 −3% 6847 Metabolite - 1496 35 0.2291 0.9846 24% 6852 Metabolite - 1498 35 0.6978 0.9846 13% 6987 Metabolite - 1573 35 0.1323 0.9846 18% 7132 Metabolite - 1667 35 0.3324 0.9846 256% 7175 Metabolite - 1655 35 0.3706 0.9846 14% 7177 Metabolite - 1656 35 0.0404 0.9846 68% 7272 Metabolite - 1679 35 0.4554 0.9846 −10% 7286 Metabolite - 1682 35 0.363 0.9846 15% 7359 n-acetyl-L-aspartic acid 35 0.7265 0.9846 −6% 7639 oxalic acid 35 0.842 0.9846 2% 7650 Metabolite - 1834 35 0.6964 0.9846 −16% 7660 Metabolite - 1839 35 0.9696 0.9875 −1% 7672 Metabolite - 1843 35 0.5216 0.9846 −6% 7933 Metabolite - 1911 35 0.219 0.9846 41% 8176 Metabolite - 1974 35 0.4332 0.9846 20% 8196 Metabolite - 1979-Cl- 35 0.0901 0.9846 96% adduct-of-isobar-19 8210 Metabolite - 1981 35 0.7764 0.9846 5% 8336 Metabolite - 2005 35 0.3343 0.9846 23% 8644 Metabolite - 2051 35 0.0878 0.9846 18% 8677 Metabolite - 2056 35 0.3734 0.9846 −12% 9007 Metabolite - 2108 35 0.2858 0.9846 −44% 9038 Metabolite - 2118 35 0.5665 0.9846 −7% 9113 Metabolite - 2133 35 0.1426 0.9846 −18% 9165 Metabolite - 2150 35 0.9275 0.9875 1% 9333 Metabolite - 2174 35 0.4734 0.9846 13% 9334 Metabolite - 2175 35 0.8327 0.9846 13% 9458 Metabolite - 2181 35 0.8365 0.9846 −3% 10058 Metabolite - 2242 35 0.3634 0.9846 427% 10087 Metabolite - 2249 35 0.3102 0.9846 −22% 10122 Metabolite - 2254 35 0.4777 0.9846 −40% 10136 Metabolite - 2034 35 0.8375 0.9846 −5% 10156 Metabolite - 2259 35 0.836 0.9846 −7% 10240 4-acetominophen-sulfate 35 0.2026 0.9846 34% 10245 Metabolite - 2269- 35 0.8222 0.9846 −2% 10247 Metabolite - 2270 35 0.6261 0.9846 15% 10252 Metabolite - 2271 35 0.2147 0.9846 30% 10286 Metabolite - 2272 35 0.4099 0.9846 −34% 10309 Metabolite - 2277 35 0.3674 0.9846 −12% 10347 Metabolite - 2285 35 0.8582 0.9846 4% 10407 Metabolite - 2059 35 0.663 0.9846 −6% 10424 Metabolite - 2292 35 0.1432 0.9846 30% 10433 Metabolite - 2293-possible- 35 0.3141 0.9846 1403% O-desmethylvenlafaxine- glucuronide 10490 Metabolite - 2319 35 0.83 0.9846 −4% 10526 Metabolite - 2323 35 0.8463 0.9846 4% 10544 Metabolite - 2329 35 0.9805 0.9875 1% 10555 Metabolite - 2348 35 0.2902 0.9846 65% 10570 Metabolite - 2366 35 0.1258 0.9846 −37% 10629 Metabolite - 2386 35 0.7455 0.9846 −4% 10644 Metabolite - 2387 35 0.3475 0.9846 −24% 10667 Metabolite - 2389 35 0.2017 0.9846 22% 10672 Metabolite - 2390 35 0.9346 0.9875 1% 10737 Isobar-1-includes- 35 0.4849 0.9846 −26% mannose-fructose-glucose- galactose-alpha-L- sorbopyranose-Inositol-D- allose-D--altrose-D- psicone 10741 Isobar-2-includes-2- 35 0.356 0.9846 −61% aminoisobutyric acid-3- amino-isobutyrate-2- amino-butyrate-4- aminobutanoic acid- dimethylglycine-choline- 10743 Isobar-4-includes-Gluconic 35 0.986 0.9883 0% acid-DL-arabinose-D- ribose-L-xylose-DL- lyxose-D-xylulose 10746 Isobar-6-includes-valine- 35 0.8873 0.9875 4% betaine 10785 Metabolite - 2506 35 0.6703 0.9846 7% 10825 Metabolite - 2546 35 0.3625 0.9846 13% 10872 Metabolite - 2550 35 0.5812 0.9846 −11% 10906 Metabolite - 2557-possible- 35 0.0411 0.9846 64% Pantoprazole-metabolite 11053 Metabolite - 2567 35 0.7296 0.9846 5% 11085 Metabolite - 2588 35 0.2272 0.9846 25% 11110 Metabolite - 2591 35 0.4451 0.9846 17% 11173 Metabolite - 2607 35 0.2724 0.9846 42% 11219 Metabolite - 2686 35 0.4501 0.9846 13% 11264 Metabolite - 2698 35 0.6617 0.9846 27% 11271 Metabolite - 2700 35 0.1367 0.9846 48% 11292 Metabolite - 2703 35 0.6289 0.9846 −6% 11299 Metabolite - 2706 35 0.2913 0.9846 −22% 11390 Metabolite - 2726 35 0.3521 0.9846 −14% 11411 Metabolite - 2746 35 0.2543 0.9846 −21% 11438 phosphate 50 0.5453 0.9846 11% 11484 Metabolite - 2752 35 0.245 0.9846 18% 11661 Metabolite - 2781 35 0.7701 0.9846 −4% 11777 glycine 50 0.5674 0.9846 10% 11808 Metabolite - 2807 35 0.0487 0.9846 62% 11851 Metabolite - 2811 35 0.0085 0.9846 161% 12025 cis-aconitic acid 50 0.3727 0.9846 58% 12055 galactose 50 0.6975 0.9846 11% 12102 o-phosphoethanolamine 50 0.6267 0.9846 −13% 12104 Metabolite - 2852 35 0.5159 0.9846 29% 12109 Metabolite - 2853 35 0.9627 0.9875 1% 12129 beta-hydroxyisovaleric 50 0.8271 0.9846 4% acid 12300 Metabolite - 2868 35 0.7519 0.9846 −16% 12358 (1′R,1′S)_biopterin 35 0.2306 0.9846 26% 12426 Metabolite - 2416 35 0.4534 0.9846 20% 12463 Metabolite - 2893-possible- 35 0.4566 0.9846 18% demethylated- Rosiglitazone 12474 Metabolite - 2897 35 0.2372 0.9846 −17% 12593 Metabolite - 2973 50 0.0377 0.9846 −25% 12641 meso-erythritol 50 0.9483 0.9875 −2% 12644 Metabolite - 3016 50 0.3011 0.9846 −8% 12648 Metabolite - 3020 50 0.8127 0.9846 5% 12666 Metabolite - 3033 50 0.8413 0.9846 3% 12711 Metabolite - 3053 35 0.5017 0.9846 24% 12720 Metabolite - 3056 35 0.5412 0.9846 8% 12765 inositol 50 0.4102 0.9846 −27% 12770 Metabolite - 3090 50 0.1121 0.9846 −11% 12771 Metabolite - 3091 50 0.2399 0.9846 −25% 12795 Metabolite - 3113 50 0.3928 0.9846 −14% 12856 Metabolite - 3123 35 0.4327 0.9846 17% 12902 Metabolite - 3127 35 0.765 0.9846 −4% 12904 Metabolite - 2457 35 0.0497 0.9846 66% 12924 Metabolite - 3131 35 0.7354 0.9846 −7% 12938 Metabolite - 2459 35 0.9267 0.9875 −1% 13018 Metabolite - 3138 35 0.9748 0.9875 0% 13136 Metabolite - 3163-possible- 35 0.9502 0.9875 1% methylcytidine- benserazide-Pyr-Gln-OH- or-glycerophosphocholine- 13153 Metabolite - 3169 35 0.6836 0.9846 13% 13179 Metabolite - 3176 35 0.8382 0.9846 −13% 13214 Metabolite - 3183-possible- 35 0.6928 0.9846 8% gamma-L-glutamyl-L- phenylalanine 13217 Metabolite - 3184 35 0.3549 0.9846 10% 13249 Metabolite - 3215 35 0.8582 0.9846 3% 13251 Metabolite - 3216 35 0.3024 0.9846 −7% 13265 Metabolite - 3221 35 0.9343 0.9875 −2% 13297 Metabolite - 3231 35 0.9212 0.9875 −2% 13318 DL-indole-3-lactic acid 35 0.4237 0.9846 −19% 13356 Metabolite - 3246-possible- 35 0.5345 0.9846 −8% Ala-GLy-glycyl-sarcosine- or-ureido-butyric acid 13459 Metabolite - 3305 35 0.483 0.9846 16% 13484 Metabolite - 3309 35 0.8589 0.9846 3% 13493 Metabolite - 3311- 35 0.1888 0.9846 56% 13505 Metabolite - 3313 35 0.5469 0.9846 −13% 13534 Metabolite - 3320-possible- 35 0.6718 0.9846 13% pimpinellin-or- tetrahydroxybenzophenone 13545 Metabolite - 3322 35 0.393 0.9846 −14% 13589 Metabolite - 3327 35 0.2554 0.9846 26% 13594 Metabolite - 3329 35 0.9464 0.9875 −2% 13704 Metabolite - 3355 35 0.2556 0.9846 20% 13744 Metabolite - 3364 35 0.2659 0.9846 19% 13775 Metabolite - 3370 35 0.5874 0.9846 12% 13791 Metabolite - 3373 35 0.985 0.9883 0% 13803 Metabolite - 3377 35 0.5535 0.9846 −22% 13817 Metabolite - 3380 35 0.3112 0.9846 −11% 13820 beta-nicotinamide- 35 0.8355 0.9846 4% mononucleotide 13847 Metabolite - 3387 35 0.7957 0.9846 4% 13904 Metabolite - 3402 35 0.3187 0.9846 71% 13968 Metabolite - 3409 35 0.3858 0.9846 18% 14036 Metabolite - 3427 35 0.7015 0.9846 8% 14066 Metabolite - 3433 35 0.7142 0.9846 −4% 14084 Metabolite - 3436 35 0.5508 0.9846 10% 14115 Metabolite - 3440 35 0.3104 0.9846 −64% 14125 Metabolite - 3443 35 0.5809 0.9846 11% 14170 Metabolite - 3457 35 0.6752 0.9846 12% 14220 Metabolite - 3470 35 0.8918 0.9875 4% 14249 Metabolite - 3476 35 0.0695 0.9846 82% 14368 Metabolite - 3489 35 0.3569 0.9846 −27% 14406 Metabolite - 3493 35 0.818 0.9846 3% 14453 Metabolite - 3507 35 0.5637 0.9846 14% 14471 Metabolite - 3516 35 0.2008 0.9846 34% 14506 Metabolite - 3543 35 0.5679 0.9846 −18% 14539 Metabolite - 3564 35 0.1262 0.9846 −38% 14595 Metabolite - 3576 35 0.4931 0.9846 9% 14640 Metabolite - 3604 35 0.1561 0.9846 20% 14641 Metabolite - 3605 35 0.1604 0.9846 44% 14731 Metabolite - 3659 35 0.4077 0.9846 18% 14732 Metabolite - 3660 35 0.2255 0.9846 31% 14733 Metabolite - 3661 35 0.7777 0.9846 −9% 14759 Metabolite - 3667 35 0.2647 0.9846 19% 14762 Metabolite - 3668 35 0.57 0.9846 −5% 14766 Metabolite - 3670 35 0.5103 0.9846 12% 14769 Metabolite - 3691 35 0.6776 0.9846 −8% 14808 Metabolite - 3701 35 0.0336 0.9846 −31% 14835 Metabolite - 3706 35 0.9101 0.9875 2% 14840 Metabolite - 3708 35 0.8511 0.9846 −6% 14907 Metabolite - 3734 35 0.3113 0.9846 16% 14983 Metabolite - 3754 35 0.9005 0.9875 −2% 14984 Metabolite - 3755 35 0.3521 0.9846 154% 15017 Metabolite - 3761 35 0.1689 0.9846 21% 15057 Metabolite - 3771 35 0.1457 0.9846 52% 15064 Metabolite - 3773 35 0.4225 0.9846 −10% 15096 N-acetyl-D-glucosamine 50 0.612 0.9846 10% 15121 Metabolite - 3786 35 0.3986 0.9846 20% 15124 porphobilinogen 35 0.8882 0.9875 2% 15125 (2- 35 0.1453 0.9846 14% Aminoethyl)phosphonate 15128 DL-homocysteine 35 0.0781 0.9846 46% 15129 D-alanyl-D-alanine 35 0.9606 0.9875 1% 15130 diaminopimelic acid 35 0.4317 0.9846 −9% 15131 dethiobiotin 35 0.7544 0.9846 −6% 15187 Metabolite - 3800 35 0.7143 0.9846 −8% 15197 Metabolite - 3802 35 0.701 0.9846 8% 15201 Metabolite - 3803 35 0.3018 0.9846 16% 15202 Metabolite - 3804 35 0.213 0.9846 −22% 15203 Metabolite - 3805 35 0.9354 0.9875 −2% 15207 Metabolite - 3806 35 0.1744 0.9846 −33% 15211 Metabolite - 3807 35 0.8708 0.9875 2% 15220 Metabolite - 3813 35 0.3635 0.9846 17% 15228 Metabolite - 3817 35 0.5239 0.9846 −12% 15240 Metabolite - 3824 35 0.8028 0.9846 −7% 15249 Metabolite - 3828 35 0.8756 0.9875 3% 15251 Metabolite - 3830 35 0.0975 0.9846 39% 15253 Metabolite - 3832-possible- 35 0.2712 0.9846 −41% phenol-sulfate 15258 Metabolite - 3834-Peptide 35 0.4949 0.9846 18% 15275 Metabolite - 3840 35 0.6594 0.9846 5% 15276 Metabolite - 3841 35 0.3623 0.9846 58% 15278 Metabolite - 3843 35 0.8581 0.9846 −4% 15284 Metabolite - 3847 35 0.9705 0.9875 0% 15294 Metabolite - 3855 35 0.8089 0.9846 5% 15312 Metabolite - 3873 35 0.609 0.9846 −8% 15315 Metabolite - 3876 35 0.379 0.9846 29% 15324 Metabolite - 3878 35 0.3307 0.9846 −18% 15326 Metabolite - 3879 35 0.9133 0.9875 −3% 15328 azelaic acid 35 0.1572 0.9846 −33% 15335 mannitol 50 0.123 0.9846 50% 15336 tartaric acid 35 0.2935 0.9846 −26% 15356 Metabolite - 3886 35 0.5807 0.9846 8% 15359 Metabolite - 3887 35 0.7867 0.9846 −5% 15365 sn-Glycerol-3-phosphate 50 0.8503 0.9846 −7% 15374 Metabolite - 3893 35 0.8837 0.9875 2% 15382 Metabolite - 3898 35 0.6325 0.9846 4% 15410 Metabolite - 3908 35 0.5194 0.9846 10% 15411 Metabolite - 3909 35 0.6385 0.9846 −23% 15418 Metabolite - 3911 35 0.4593 0.9846 8% 15496 agmatine 35 0.0364 0.9846 34% 15500 carnitine 35 0.7459 0.9846 10% 15529 Metabolite - 3951 35 0.9957 0.9957 0% 15532 Metabolite - 3952 35 0.4244 0.9846 −16% 15535 Metabolite - 3955 35 0.9603 0.9875 1% 15541 Metabolite - 3957 35 0.8287 0.9846 −3% 15599 Metabolite - 3963 35 0.3146 0.9846 24% 15610 Metabolite - 3970 35 0.4461 0.9846 17% 15620 Metabolite - 3973 35 0.3553 0.9846 44% 15626 Metabolite - 3977 35 0.1456 0.9846 −35% 15636 Metabolite - 3981 35 0.5962 0.9846 −16% 15641 Metabolite - 3986 35 0.6453 0.9846 −6% 15650 1-methyladenosine 35 0.8651 0.9875 −2% 15667 2-isopropylmalic acid 50 0.6338 0.9846 −24% 15676 3-methyl-2-oxovaleric acid 35 0.4947 0.9846 16% 15677 3-methyl-L-histidine 35 0.046 0.9846 −23% 15679 xanthurenic acid 50 0.5776 0.9846 14% 15681 4-Guanidinobutanoic acid 35 0.8135 0.9846 4% 15704 heptanedioic acid 35 0.7689 0.9846 −3% 15716 L-beta-imidazolelactic acid 50 0.5938 0.9846 18% 15730 suberic acid 35 0.2788 0.9846 −13% 15737 hydroxyacetic acid 50 0.5729 0.9846 −9% 15743 N-N-dimethylarginine 35 0.7457 0.9846 4% 15753 hippuric acid 35 0.4325 0.9846 14% 15778 benzoic acid 35 0.1887 0.9846 63% 15804 maltose 50 0.428 0.9846 64% 15835 L-xylose 50 0.7767 0.9846 10% 15948 S-adenosyl-l-homocysteine 35 0.6109 0.9846 −11% 15964 D-arabitol 50 0.9713 0.9875 1% 16002 Metabolite - 3992-possible- 35 0.1682 0.9846 16% Cl-adduct-of-Formate- dimer 16016 Metabolite - 3994 35 0.1703 0.9846 −24% 16034 Metabolite - 4002 50 0.1968 0.9846 30% 16071 Metabolite - 4020 50 0.6192 0.9846 8% 16082 Metabolite - 4027 50 0.7798 0.9846 5% 16107 lysine 50 0.2773 0.9846 145% 16175 Metabolite - 4092 35 0.4841 0.9846 −13% 16197 Metabolite - 4112 35 0.8282 0.9846 4% 16217 Metabolite - 4116 35 0.8574 0.9846 −6% 16230 Isobar-29-includes-R-S- 35 0.0326 0.9846 114% hydroorotic acid-5-6- dihydroorotic acid 16232 Isobar-17-includes- 35 0.7984 0.9846 5% arginine-N-alpha-acetyl- ornithine 16233 Isobar-13-includes-5-keto- 35 0.7037 0.9846 −8% D-gluconic acid-2-keto-L- gulonic acid-D-glucuronic acid-D-galacturonic acid 16235 Isobar-19-includes-D- 35 0.1465 0.9846 66% saccharic acid-1-5- anhydro-D-glucitol-2- deoxy-D-galactose-2- deoxy-D-glucose-L-fucose- L-rhamnose 16243 L-kynurenine 35 0.5787 0.9846 −8% 16276 Isobar-38-includes-N- 35 0.4495 0.9846 19% acetyl-L-methionine-5- hydroxy-1H-indole-3- acetic acid 16278 Isobar-35-includes-D- 35 0.1346 0.9846 20% arabinose-5-phosphate-D- ribulose-5-phosphate- alpha-D-ribose-5- phosphate 16290 Metabolite - 4133 50 0.4411 0.9846 35% 16337 Metabolite - 4167 35 0.633 0.9846 12% 16338 Metabolite - 4168 35 0.6584 0.9846 20% 16457 Metabolite - 4233 35 0.4705 0.9846 21% 16462 Metabolite - 4234 35 0.1194 0.9846 57% 16496 Metabolite - 4251 50 0.1706 0.9846 −16% 16506 Metabolite - 4271 50 0.2282 0.9846 31% 16816 Metabolite - 4494 50 0.938 0.9875 −1% 16818 Metabolite - 4495 50 0.3478 0.9846 18% 16819 Metabolite - 4496 50 0.4825 0.9846 6% 16821 Metabolite - 4498 50 0.2269 0.9846 34% 16822 Metabolite - 4499 50 0.5555 0.9846 11% 16823 Metabolite - 4500 50 0.3974 0.9846 63% 16824 iminodiacetic acid 50 0.1445 0.9846 −23% 16827 Metabolite - 4502 50 0.6363 0.9846 −1% 16829 Metabolite - 4503 50 0.2869 0.9846 23% 16831 Metabolite - 4504 50 0.7348 0.9846 6% 16834 Metabolite - 4505 50 0.6099 0.9846 15% 16837 Metabolite - 4507 50 0.2767 0.9846 26% 16848 Metabolite - 4511 50 0.3715 0.9846 33% 16851 Metabolite - 4512 50 0.1504 0.9846 59% 16859 Metabolite - 4516 50 0.6517 0.9846 8% 16860 Metabolite - 4517 50 0.7046 0.9846 10% 16861 Metabolite - 4518 50 0.9422 0.9875 1% 16862 Metabolite - 4519 50 0.7079 0.9846 −11% 16863 Metabolite - 4520 50 0.9455 0.9875 −2% 16864 Metabolite - 4521 50 0.8773 0.9875 −5% 16865 Metabolite - 4522 50 0.6761 0.9846 7% 16866 Metabolite - 4523 50 0.7153 0.9846 6% 16867 Metabolite - 4524 50 0.2787 0.9846 −31% 16952 Metabolite - 4593 50 0.2049 0.9846 18% 16959 Metabolite - 4595 50 0.7468 0.9846 3% 17028 Metabolite - 4611 50 0.872 0.9875 3% 17050 Metabolite - 4618 50 0.2699 0.9846 72% 17064 Metabolite - 4624 50 0.3903 0.9846 13% 17072 Metabolite - 4628 50 0.2516 0.9846 81% 17074 Metabolite - 4629 50 0.6487 0.9846 9% 17080 Metabolite - 4632 50 0.8257 0.9846 7% 17083 Metabolite - 4634 50 0.7731 0.9846 6% 17084 Metabolite - 4635 50 0.3361 0.9846 134% 17085 Metabolite - 4636 50 0.5178 0.9846 18% 17086 Metabolite - 4637 50 0.834 0.9846 7% 17087 Metabolite - 4638 50 0.2699 0.9846 44% 17088 Metabolite - 4639 50 0.3228 0.9846 32%

TABLE 10 Urine Metabolite Biomarkers to distinguish Non-cancer from Higher Grade PCA. % Change COMP_ID COMPOUND LIB_ID p-value q-value in PCA 53 glutamine 50 0.2634 0.2182 39% 54 tryptophan 35 0.1769 0.1876 25% 57 glutamic acid 50 0.3771 0.2666 18% 59 histidine 50 0.1191 0.1705 61% 60 leucine 50 0.0862 0.1546 58% 64 phenylalanine 35 0.0753 0.1546 33% 418 guanine 50 0.0722 0.1546 56% 512 asparagine 50 0.1886 0.1931 45% 513 creatinine 35 0.1327 0.1736 21% 521 homogentisate 50 0.1389 0.1744 59% 527 lactate 50 0.4598 0.2867 −11%  528 alpha-keto-glutarate 35 0.7074 0.3708 12% 531 3-hydroxy-3- 50 0.378 0.2666 24% methylglutarate 541 4-hydroxyphenylacetate 50 0.2908 0.2279 32% 542 3-hydroxybutanoic acid 50 0.1308 0.1723 −48%  554 adenine 50 0.0464 0.1546 55% 555 adenosine 35 0.0068 0.1546 84% 563 alpha-L-sorbopyranose 50 0.2249 0.2026 92% 569 caffeine 35 0.0527 0.1546 −43%  575 arabinose 50 0.0227 0.1546 88% 577 fructose 50 0.4822 0.2929 41% 581 glucose 50 0.4136 0.2782 −65%  587 gluconic acid 50 0.0697 0.1546 86% 594 niacinamide 35 0.6385 0.3496 −8% 597 phosphoenolpyruvate 35 0.6788 0.3622 20% 605 uracil 50 0.0194 0.1546 55% 607 urocanic acid 35 0.0836 0.1546 54% 608 vitamin-B6 35 0.0887 0.1575 79% 1101 3-methoxy-4- 50 0.0139 0.1546 57% hydroxyphenylacetate 1107 allantoin 50 0.0457 0.1546 83% 1125 isoleucine 50 0.3971 0.272 16% 1126 alanine 50 0.2068 0.1964 36% 1284 threonine 50 0.0516 0.1546 45% 1299 tyrosine 50 0.0503 0.1546 45% 1302 methionine 35 0.0169 0.1546 45% 1303 malic acid 35 0.5118 0.3041 18% 1366 trans-4-hydroxyproline 50 0.229 0.2026 32% 1413 3-hydroxyphenylacetate 35 0.671 0.36 12% 1417 kynurenic acid 50 0.172 0.1874 66% 1418 5-6-Dihydrothymine 35 0.0782 0.1546 44% 1419 5-s-methyl-5- 35 0.9786 0.4388  0% thioadenosine 1431 (p-Hydroxyphenyl)lactic 50 0.2483 0.2111 87% acid 1432 alphahydroxybenzeneacetic 35 0.7995 0.3952  4% acid 1437 succinate 50 0.0769 0.1546 62% 1444 DL-pipecolic acid 35 0.728 0.3756 13% 1480 guanidineacetic acid 35 0.0049 0.1546 184%  1493 ornithine 50 0.062 0.1546 52% 1494 5-oxoproline 50 0.1124 0.1698 42% 1498 N-6-trimethyl-l-lysine 35 0.0433 0.1546 62% 1505 orotic acid 50 0.0559 0.1546 74% 1508 pantothenic acid 35 0.3273 0.2426 56% 1519 sucrose 50 0.102 0.1641 95% 1557 3-methylglutaric acid 35 0.6501 0.3538 −9% 1558 4-acetamidobutyric acid 35 0.1142 0.1698 39% 1559 5-6-dihydrouracil 50 0.0325 0.1546 44% 1560 L-methyldopa 35 0.2445 0.2093 36% 1564 citric acid 50 0.0304 0.1546 62% 1566 3-amino-isobutyrate 50 0.1884 0.1931 64% 1567 4-hydroxy-3- 50 0.0913 0.1595 48% methoxymandelate 1568 4-hydroxymandelate 50 0.1129 0.1698 62% 1569 DL-beta- 35 0.1404 0.1744 36% hydroxyphenylethylamine 1574 histamine 35 0.0267 0.1546 57% 1580 noradrenaline 50 0.1426 0.1744 67% 1585 N-acetyl-L-alanine 35 0.8018 0.3953  5% 1587 N-acetyl-L-leucine 35 0.1032 0.1641 49% 1591 N-acetyl-L-valine 35 0.0682 0.1546 101%  1592 N-acetylneuraminic acid 50 0.035 0.1546 46% 1598 N-tigloylglycine 35 0.0515 0.1546 46% 1604 uric acid 35 0.243 0.2093  6% 1640 ascorbic acid 50 0.3011 0.2324 228%  1648 serine 50 0.016 0.1546 60% 1649 valine 50 0.0849 0.1546 31% 1708 7-8-dihydrofolic acid 35 0.0656 0.1546 36% 1778 gamma-glu-cys 35 0.4751 0.2914 45% 1827 riboflavine 35 0.1656 0.1865 264%  1860 3-nitro-L-tyrosine 35 0.4409 0.2848 25% 1868 cysteine 50 0.1193 0.1705 108%  1898 proline 50 0.3203 0.2413 39% 1899 quinolinic acid 50 0.0925 0.1595 44% 2078 pyrophosphate 50 0.0839 0.1546 107%  2092 catechol 35 0.0165 0.1546 151%  2132 citrulline 50 0.0064 0.1546 73% 2183 thymidine 35 0.8414 0.4032 −4% 2245 Metabolite - 294 35 0.1285 0.1705 89% 2342 serotonin 35 0.0389 0.1546 41% 2734 gamma-L-glutamyl-L- 35 0.3139 0.2394 26% tyrosine 2829 N-formyl-L-methionine 35 0.1969 0.195 46% 2831 adenosine-3-5-cyclic- 35 0.0306 0.1546 32% monophosphate 3127 hypoxanthine 35 0.1978 0.195 56% 3138 pyridoxamine-phosphate 35 0.752 0.3841 −5% 3147 xanthine 35 0.148 0.1787 77% 3155 3-ureidopropionic acid 35 0.069 0.1546 96% 4966 xylitol 50 0.7869 0.3926 −5% 5493 Metabolite - 1059 35 0.9512 0.4306  1% 5495 Metabolite - 1060 35 0.1027 0.1641 −19%  5514 Metabolite - 1081 35 0.008 0.1546 11% 5538 Metabolite - 1101 35 0.6862 0.363 26% 5664 Metabolite - 1101 35 0.7561 0.3849  6% 5687 Metabolite - 1110 35 0.4575 0.2863 16% 5697 acetylcarnitine- 35 0.9057 0.4171  4% 5702 Metabolite - 1114 35 0.477 0.2917 27% 5711 2-hydroxybutyric acid 35 0.9468 0.4297  2% 5719 Metabolite - 1122 35 0.1964 0.195 24% 5727 Metabolite - 1126 35 0.7746 0.3891  8% 5797 Metabolite - 1186 35 0.6024 0.3354 12% 6137 Metabolite - 1212 35 0.479 0.2919 −32%  6147 Metabolite - 1216 35 0.7457 0.3827 −5% 6238 normetanephrine 50 0.2267 0.2026 13% 6253 Metabolite - 1283 35 0.9728 0.4373  1% 6278 Metabolite - 1289 35 0.1762 0.1876 17% 6329 urea 50 0.1643 0.1865 27% 6362 Metabolite - 1323- 35 0.0787 0.1546 84% possible-p-cresol-sulfate 6398 Metabolite - 1335 35 0.1991 0.195 25% 6405 Metabolite - 1338 35 0.3289 0.2426 −21%  6413 Metabolite - 1342- 35 0.0596 0.1546 40% possible- phenylacetylglutamine- or-formyl-N-acetyl-5- methoxykynurenamine 6421 Metabolite - 1345 35 0.1218 0.1705 105%  6437 Metabolite - 1349- 35 0.0435 0.1546 54% possible-N-acetyl-8-O- methyl-Neuraminic acid 6443 Metabolite - 1351 35 0.5184 0.3061 26% 6477 Metabolite - 1364 35 0.8749 0.413 −6% 6486 Metabolite - 1368 35 0.7964 0.3952 13% 6493 salicyluric acid 35 0.3168 0.2401 243%  6528 Metabolite - 1383- 35 0.1243 0.1705 97% possible-salicyluric- glucuronide 6760 Metabolite - 1455 35 0.1951 0.195 23% 6764 Metabolite - 1459 35 0.386 0.2688 54% 6777 Metabolite - 1463 35 0.3817 0.2674 79% 6787 Metabolite - 1465 35 0.3552 0.2553 16% 6847 Metabolite - 1496 35 0.4314 0.2831 43% 6852 Metabolite - 1498 35 0.2973 0.2314 67% 6987 Metabolite - 1573 35 0.0136 0.1546 38% 7132 Metabolite - 1667 35 0.9013 0.4171 −3% 7175 Metabolite - 1655 35 0.0662 0.1546 103%  7177 Metabolite - 1656 35 0.0932 0.1595 106%  7272 Metabolite - 1679 35 0.2252 0.2026 28% 7286 Metabolite - 1682 35 0.4881 0.2937 19% 7359 n-acetyl-L-aspartic acid 35 0.0973 0.1641 56% 7639 oxalic acid 35 0.1037 0.1641 28% 7650 Metabolite - 1834 35 0.3481 0.2512 −34%  7660 Metabolite - 1839 35 0.9015 0.4171 −4% 7672 Metabolite - 1843 35 0.2013 0.1955 21% 7933 Metabolite - 1911 35 0.0604 0.1546 100%  8176 Metabolite - 1974 35 0.0348 0.1546 71% 8196 Metabolite - 1979-Cl- 35 0.0609 0.1546 77% adduct-of-isobar-19 8210 Metabolite - 1981 35 0.7647 0.3861  6% 8336 Metabolite - 2005 35 0.6452 0.3522 10% 8644 Metabolite - 2051 35 0.041 0.1546 27% 8677 Metabolite - 2056 35 0.5849 0.3294  9% 9007 Metabolite - 2108 35 0.4471 0.2848 −34%  9038 Metabolite - 2118 35 0.3323 0.2426 20% 9113 Metabolite - 2133 35 0.6941 0.3651  8% 9165 Metabolite - 2150 35 0.3174 0.2401 30% 9333 Metabolite - 2174 35 0.2248 0.2026 29% 9334 Metabolite - 2175 35 0.2046 0.1961 95% 9458 Metabolite - 2181 35 0.0134 0.1546 52% 10058 Metabolite - 2242 35 0.0104 0.1546 −55%  10087 Metabolite - 2249 35 0.4419 0.2848 39% 10122 Metabolite - 2254 35 0.4675 0.2896 −41%  10136 Metabolite - 2034 35 0.4115 0.2778 35% 10156 Metabolite - 2259 35 0.3889 0.2693 71% 10240 4-acetominophen-sulfate 35 0.1068 0.1675 54% 10245 Metabolite - 2269- 35 0.8384 0.4028  3% 10247 Metabolite - 2270 35 0.2488 0.2111 −42%  10252 Metabolite - 2271 35 0.0323 0.1546 79% 10286 Metabolite - 2272 35 0.7248 0.375 19% 10309 Metabolite - 2277 35 0.0567 0.1546 39% 10347 Metabolite - 2285 35 0.0852 0.1546 50% 10407 Metabolite - 2059 35 0.4659 0.2896 14% 10424 Metabolite - 2292 35 0.3819 0.2674 −17%  10433 Metabolite - 2293- 35 0.1642 0.1865 35% possible-O- desmethylvenlafaxine- glucuronide 10490 Metabolite - 2319 35 0.8212 0.3991  4% 10526 Metabolite - 2323 35 0.713 0.372 11% 10544 Metabolite - 2329 35 0.4846 0.2934 12% 10555 Metabolite - 2348 35 0.8929 0.4163  5% 10570 Metabolite - 2366 35 0.2076 0.1964 −29%  10629 Metabolite - 2386 35 0.4286 0.2831 14% 10644 Metabolite - 2387 35 0.8787 0.4132  6% 10667 Metabolite - 2389 35 0.7983 0.3952 −5% 10672 Metabolite - 2390 35 0.0662 0.1546 70% 10737 Isobar-1-includes- 35 0.4454 0.2848 −28%  mannose-fructose- glucose-galactose-alpha- L-sorbopyranose-Inositol- D-allose-D--altrose-D- psicone 10741 Isobar-2-includes-2- 35 0.3313 0.2426 −65%  aminoisobutyric acid-3- amino-isobutyrate-2- amino-butyrate-4- aminobutanoic acid- dimethylglycine-choline- 10743 Isobar-4-includes- 35 0.0302 0.1546 48% Gluconic acid-DL- arabinose-D-ribose-L- xylose-DL-lyxose-D- xylulose 10746 Isobar-6-includes-valine- 35 0.3058 0.2341 19% betaine 10785 Metabolite - 2506 35 0.6343 0.3485 11% 10825 Metabolite - 2546 35 0.119 0.1705 29% 10872 Metabolite - 2550 35 0.1693 0.1865 77% 10906 Metabolite - 2557- 35 0.1732 0.1874 51% possible-Pantoprazole- metabolite 11053 Metabolite - 2567 35 0.0188 0.1546 49% 11085 Metabolite - 2588 35 0.2532 0.2129 105%  11110 Metabolite - 2591 35 0.2696 0.2198 −24%  11173 Metabolite - 2607 35 0.2777 0.2216 59% 11219 Metabolite - 2686 35 0.4301 0.2831 18% 11264 Metabolite - 2698 35 0.5771 0.3274 −27%  11271 Metabolite - 2700 35 0.116 0.1705 91% 11292 Metabolite - 2703 35 0.2307 0.2026 17% 11299 Metabolite - 2706 35 0.8211 0.3991 −6% 11390 Metabolite - 2726 35 0.5251 0.308 13% 11411 Metabolite - 2746 35 0.1037 0.1641 −29%  11438 phosphate 50 0.0685 0.1546 42% 11484 Metabolite - 2752 35 0.0063 0.1546 60% 11661 Metabolite - 2781 35 0.1145 0.1698 25% 11777 glycine 50 0.0284 0.1546 58% 11808 Metabolite - 2807 35 0.1406 0.1744 64% 11851 Metabolite - 2811 35 0.026 0.1546 235%  12025 cis-aconitic acid 50 0.1274 0.1705 76% 12055 galactose 50 0.7432 0.3824 14% 12102 o-phosphoethanolamine 50 0.7644 0.3861  8% 12104 Metabolite - 2852 35 0.1765 0.1876 285%  12109 Metabolite - 2853 35 0.6347 0.3485 15% 12129 beta-hydroxyisovaleric 50 0.066 0.1546 49% acid 12300 Metabolite - 2868 35 0.4388 0.2848 76% 12358 (1′R,1′S)_biopterin 35 0.1576 0.1865 30% 12426 Metabolite - 2416 35 0.1652 0.1865 72% 12463 Metabolite - 2893- 35 0.4067 0.2755 27% possible-demethylated- Rosiglitazone 12474 Metabolite - 2897 35 0.8628 0.4093 −4% 12593 Metabolite - 2973 50 0.1595 0.1865 −22%  12641 meso-erythritol 50 0.3364 0.2437 28% 12644 Metabolite - 3016 50 0.72 0.3736 −4% 12648 Metabolite - 3020 50 0.0217 0.1546 69% 12666 Metabolite - 3033 50 0.0719 0.1546 51% 12711 Metabolite - 3053 35 0.788 0.3926 14% 12720 Metabolite - 3056 35 0.0489 0.1546 41% 12765 inositol 50 0.4194 0.2789 −27%  12770 Metabolite - 3090 50 0.3345 0.2432 12% 12771 Metabolite - 3091 50 0.3988 0.2721 37% 12795 Metabolite - 3113 50 0.5631 0.3223 −15%  12856 Metabolite - 3123 35 0.7525 0.3841  8% 12902 Metabolite - 3127 35 0.6832 0.363 10% 12904 Metabolite - 2457 35 0.169 0.1865 83% 12924 Metabolite - 3131 35 0.4335 0.2834 22% 12938 Metabolite - 2459 35 0.6893 0.3637 10% 13018 Metabolite - 3138 35 0.669 0.36 12% 13136 Metabolite - 3163- 35 0.2764 0.2216 19% possible-methylcytidine- benserazide-Pyr-Gln-OH- or- glycerophosphocholine- 13153 Metabolite - 3169 35 0.2308 0.2026 162%  13179 Metabolite - 3176 35 0.8221 0.3991 14% 13214 Metabolite - 3183- 35 0.0768 0.1546 57% possible-gamma-L- glutamyl-L-phenylalanine 13217 Metabolite - 3184 35 0.0416 0.1546 51% 13249 Metabolite - 3215 35 0.0164 0.1546 45% 13251 Metabolite - 3216 35 0.2086 0.1964  9% 13265 Metabolite - 3221 35 0.2654 0.2182 31% 13297 Metabolite - 3231 35 0.8602 0.4091  4% 13318 DL-indole-3-lactic acid 35 0.7643 0.3861 −9% 13356 Metabolite - 3246- 35 0.0822 0.1546 62% possible-Ala-GLy-glycyl- sarcosine-or-ureido- butyric acid 13459 Metabolite - 3305 35 0.0755 0.1546 71% 13484 Metabolite - 3309 35 0.3911 0.2698 17% 13493 Metabolite - 3311- 35 0.837 0.4028 −6% 13505 Metabolite - 3313 35 0.9682 0.4363  1% 13534 Metabolite - 3320- 35 0.4164 0.2789 51% possible-pimpinellin-or- tetrahydroxybenzophenone 13545 Metabolite - 3322 35 0.1275 0.1705 63% 13589 Metabolite - 3327 35 0.085 0.1546 74% 13594 Metabolite - 3329 35 0.0641 0.1546 145%  13704 Metabolite - 3355 35 0.1883 0.1931 33% 13744 Metabolite - 3364 35 0.8656 0.4096 −3% 13775 Metabolite - 3370 35 0.0505 0.1546 51% 13791 Metabolite - 3373 35 0.3231 0.2424 1137%  13803 Metabolite - 3377 35 0.882 0.4132  7% 13817 Metabolite - 3380 35 0.1975 0.195 36% 13820 beta-nicotinamide- 35 0.0287 0.1546 51% mononucleotide 13847 Metabolite - 3387 35 0.2135 0.2001 28% 13904 Metabolite - 3402 35 0.6861 0.363  6% 13968 Metabolite - 3409 35 0.5164 0.3059 22% 14036 Metabolite - 3427 35 0.0743 0.1546 72% 14066 Metabolite - 3433 35 0.5622 0.3223  7% 14084 Metabolite - 3436 35 0.5088 0.3032 13% 14115 Metabolite - 3440 35 0.2994 0.232 −66%  14125 Metabolite - 3443 35 0.0258 0.1546 −49%  14170 Metabolite - 3457 35 0.9959 0.4445  0% 14220 Metabolite - 3470 35 0.0581 0.1546 91% 14249 Metabolite - 3476 35 0.0267 0.1546 66% 14368 Metabolite - 3489 35 0.1102 0.1698 −48%  14406 Metabolite - 3493 35 0.3257 0.2426 21% 14453 Metabolite - 3507 35 0.774 0.3891  8% 14471 Metabolite - 3516 35 0.7774 0.3894  6% 14506 Metabolite - 3543 35 0.4448 0.2848 73% 14539 Metabolite - 3564 35 0.5935 0.3317 29% 14595 Metabolite - 3576 35 0.1252 0.1705 78% 14640 Metabolite - 3604 35 0.4348 0.2834 20% 14641 Metabolite - 3605 35 0.1679 0.1865 78% 14731 Metabolite - 3659 35 0.182 0.1904 34% 14732 Metabolite - 3660 35 0.3269 0.2426 64% 14733 Metabolite - 3661 35 0.5283 0.308 17% 14759 Metabolite - 3667 35 0.2604 0.217 24% 14762 Metabolite - 3668 35 0.473 0.2914 −11%  14766 Metabolite - 3670 35 0.6732 0.3602  8% 14769 Metabolite - 3691 35 0.0229 0.1546 116%  14808 Metabolite - 3701 35 0.716 0.3725 11% 14835 Metabolite - 3706 35 0.2566 0.2148 21% 14840 Metabolite - 3708 35 0.2166 0.2008 166%  14907 Metabolite - 3734 35 0.0383 0.1546 73% 14983 Metabolite - 3754 35 0.9142 0.4199 −3% 14984 Metabolite - 3755 35 0.9378 0.4275  1% 15017 Metabolite - 3761 35 0.029 0.1546 40% 15057 Metabolite - 3771 35 0.4518 0.2856 38% 15064 Metabolite - 3773 35 0.8169 0.3991  5% 15096 N-acetyl-D-glucosamine 50 0.016 0.1546 100%  15121 Metabolite - 3786 35 0.5899 0.3306 17% 15124 porphobilinogen 35 0.2695 0.2198 25% 15125 (2- 35 0.0023 0.1546 42% Aminoethyl)phosphonate 15128 DL-homocysteine 35 0.0263 0.1546 76% 15129 D-alanyl-D-alanine 35 0.1999 0.195 28% 15130 diaminopimelic acid 35 0.1669 0.1865 36% 15131 dethiobiotin 35 0.4736 0.2914 64% 15187 Metabolite - 3800 35 0.1387 0.1744 68% 15197 Metabolite - 3802 35 0.2646 0.2182 50% 15201 Metabolite - 3803 35 0.049 0.1546 69% 15202 Metabolite - 3804 35 0.3305 0.2426 26% 15203 Metabolite - 3805 35 0.18 0.1894 29% 15207 Metabolite - 3806 35 0.5382 0.3128 31% 15211 Metabolite - 3807 35 0.0225 0.1546 33% 15220 Metabolite - 3813 35 0.083 0.1546 48% 15228 Metabolite - 3817 35 0.14 0.1744 43% 15240 Metabolite - 3824 35 0.9991 0.4448  1% 15249 Metabolite - 3828 35 0.1281 0.1705 48% 15251 Metabolite - 3830 35 0.1474 0.1787 67% 15253 Metabolite - 3832- 35 0.2771 0.2216 −40%  possible-phenol-sulfate 15258 Metabolite - 3834-Peptide 35 0.2916 0.2279 50% 15275 Metabolite - 3840 35 0.419 0.2789 17% 15276 Metabolite - 3841 35 0.2765 0.2216 39% 15278 Metabolite - 3843 35 0.2183 0.2008 42% 15284 Metabolite - 3847 35 0.9603 0.4337  2% 15294 Metabolite - 3855 35 0.9398 0.4275  2% 15312 Metabolite - 3873 35 0.2025 0.1956 30% 15315 Metabolite - 3876 35 0.8981 0.4171  7% 15324 Metabolite - 3878 35 0.4489 0.2848 −19%  15326 Metabolite - 3879 35 0.5546 0.3194 36% 15328 azelaic acid 35 0.6594 0.3573 −15%  15335 mannitol 50 0.1881 0.1931 65% 15336 tartaric acid 35 0.5703 0.3245 24% 15356 Metabolite - 3886 35 0.2185 0.2008 26% 15359 Metabolite - 3887 35 0.9902 0.443  0% 15365 sn-Glycerol-3-phosphate 50 0.6605 0.3573 14% 15374 Metabolite - 3893 35 0.4206 0.2789 18% 15382 Metabolite - 3898 35 0.1721 0.1874 19% 15410 Metabolite - 3908 35 0.0752 0.1546 65% 15411 Metabolite - 3909 35 0.4575 0.2863 −35%  15418 Metabolite - 3911 35 0.2886 0.2274 16% 15496 agmatine 35 0.5278 0.308 25% 15500 carnitine 35 0.3618 0.2584 −24%  15529 Metabolite - 3951 35 0.0523 0.1546 34% 15532 Metabolite - 3952 35 0.5521 0.319 25% 15535 Metabolite - 3955 35 0.0719 0.1546 72% 15541 Metabolite - 3957 35 0.8591 0.4091  3% 15599 Metabolite - 3963 35 0.4048 0.2752 114%  15610 Metabolite - 3970 35 0.0812 0.1546 45% 15620 Metabolite - 3973 35 0.1635 0.1865 179%  15626 Metabolite - 3977 35 0.8247 0.3993  7% 15636 Metabolite - 3981 35 0.6037 0.3354 25% 15641 Metabolite - 3986 35 0.9057 0.4171  2% 15650 1-methyladenosine 35 0.0502 0.1546 51% 15667 2-isopropylmalic acid 50 0.5859 0.3294 −27%  15676 3-methyl-2-oxovaleric 35 0.8809 0.4132  5% acid 15677 3-methyl-L-histidine 35 0.3622 0.2584 16% 15679 xanthurenic acid 50 0.2292 0.2026 43% 15681 4-Guanidinobutanoic acid 35 0.1143 0.1698 38% 15704 heptanedioic acid 35 0.0573 0.1546 38% 15716 L-beta-imidazolelactic 50 0.2314 0.2026 55% acid 15730 suberic acid 35 0.9187 0.421 −3% 15737 hydroxyacetic acid 50 0.2051 0.1961 26% 15743 N-N-dimethylarginine 35 0.1269 0.1705 30% 15753 hippuric acid 35 0.2825 0.2245 26% 15778 benzoic acid 35 0.8138 0.3991  6% 15804 maltose 50 0.8907 0.4163 −6% 15835 L-xylose 50 0.3032 0.233 61% 15948 S-adenosyl-l- 35 0.3769 0.2666 24% homocysteine 15964 D-arabitol 50 0.0555 0.1546 53% 16002 Metabolite - 3992- 35 0.6621 0.3573  6% possible-Cl-adduct-of- Formate-dimer 16016 Metabolite - 3994 35 0.1773 0.1876 44% 16034 Metabolite - 4002 50 0.0999 0.1641 53% 16071 Metabolite - 4020 50 0.0376 0.1546 55% 16082 Metabolite - 4027 50 0.1234 0.1705 39% 16107 lysine 50 0.2204 0.2015 72% 16175 Metabolite - 4092 35 0.8448 0.4038  6% 16197 Metabolite - 4112 35 0.1002 0.1641 50% 16217 Metabolite - 4116 35 0.6325 0.3485 22% 16230 Isobar-29-includes-R-S- 35 0.0858 0.1546 404%  hydroorotic acid-5-6- dihydroorotic acid 16232 Isobar-17-includes- 35 0.502 0.3001 −19%  arginine-N-alpha-acetyl- ornithine 16233 Isobar-13-includes-5- 35 0.4534 0.2856 49% keto-D-gluconic acid-2- keto-L-gulonic acid-D- glucuronic acid-D- galacturonic acid 16235 Isobar-19-includes-D- 35 0.0455 0.1546 103%  saccharic acid-1-5- anhydro-D-glucitol-2- deoxy-D-galactose-2- deoxy-D-glucose-L- fucose-L-rhamnose 16243 L-kynurenine 35 0.2729 0.2215 316%  16276 Isobar-38-includes-N- 35 0.8281 0.3999 −4% acetyl-L-methionine-5- hydroxy-1H-indole-3- acetic acid 16278 Isobar-35-includes-D- 35 0.585 0.3294 16% arabinose-5-phosphate-D- ribulose-5-phosphate- alpha-D-ribose-5- phosphate 16290 Metabolite - 4133 50 0.5451 0.3159 26% 16337 Metabolite - 4167 35 0.6297 0.3485 11% 16338 Metabolite - 4168 35 0.2445 0.2093 239%  16457 Metabolite - 4233 35 0.0367 0.1546 160%  16462 Metabolite - 4234 35 0.3867 0.2688 105%  16496 Metabolite - 4251 50 0.0612 0.1546 68% 16506 Metabolite - 4271 50 0.2168 0.2008 42% 16816 Metabolite - 4494 50 0.3938 0.2707 −8% 16818 Metabolite - 4495 50 0.2517 0.2126 28% 16819 Metabolite - 4496 50 0.1967 0.195 17% 16821 Metabolite - 4498 50 0.1936 0.195 37% 16822 Metabolite - 4499 50 0.0625 0.1546 57% 16823 Metabolite - 4500 50 0.2443 0.2093 159%  16824 iminodiacetic acid 50 0.5004 0.3001 23% 16827 Metabolite - 4502 50 0.0748 0.1546  5% 16829 Metabolite - 4503 50 0.0266 0.1546 60% 16831 Metabolite - 4504 50 0.012 0.1546 56% 16834 Metabolite - 4505 50 0.1015 0.1641 93% 16837 Metabolite - 4507 50 0.4492 0.2848 27% 16848 Metabolite - 4511 50 0.1687 0.1865 62% 16851 Metabolite - 4512 50 0.7087 0.3708 20% 16859 Metabolite - 4516 50 0.1197 0.1705 111%  16860 Metabolite - 4517 50 0.8131 0.3991  7% 16861 Metabolite - 4518 50 0.5277 0.308 47% 16862 Metabolite - 4519 50 0.0624 0.1546 120%  16863 Metabolite - 4520 50 0.4865 0.2937 −24%  16864 Metabolite - 4521 50 0.5658 0.3229 −21%  16865 Metabolite - 4522 50 0.0149 0.1546 54% 16866 Metabolite - 4523 50 0.0831 0.1546 43% 16867 Metabolite - 4524 50 0.9395 0.4275 −3% 16952 Metabolite - 4593 50 0.0167 0.1546 56% 16959 Metabolite - 4595 50 0.1416 0.1744 30% 17028 Metabolite - 4611 50 0.1674 0.1865 35% 17050 Metabolite - 4618 50 0.1343 0.1744 47% 17064 Metabolite - 4624 50 0.1424 0.1744 29% 17072 Metabolite - 4628 50 0.1106 0.1698 133%  17074 Metabolite - 4629 50 0.1355 0.1744 44% 17080 Metabolite - 4632 50 0.2873 0.2274 75% 17083 Metabolite - 4634 50 0.2324 0.2026 39% 17084 Metabolite - 4635 50 0.1653 0.1865 120%  17085 Metabolite - 4636 50 0.0414 0.1546 77% 17086 Metabolite - 4637 50 0.1528 0.1833 114%  17087 Metabolite - 4638 50 0.1281 0.1705 97% 17088 Metabolite - 4639 50 0.0915 0.1595 109% 

TABLE 11 Urine Metabolite Biomarkers to distinguish Lower Grade PCA from Higher Grade PCA. % Change in Higher COMP_ID COMPOUND LIB_ID p-value q-value PCA 53 glutamine 50 0.3616 0.4198 32% 54 tryptophan 35 0.0325 0.3021 47% 57 glutamic acid 50 0.3185 0.411 21% 59 histidine 50 0.2782 0.3951 37% 60 leucine 50 0.1497 0.3451 46% 64 phenylalanine 35 0.0392 0.3021 42% 418 guanine 50 0.0948 0.3377 54% 512 asparagine 50 0.1243 0.3401 60% 513 creatinine 35 0.0268 0.3021 34% 521 homogentisate 50 0.1224 0.3401 67% 527 lactate 50 0.9146 0.6009 −2% 528 alpha-keto-glutarate 35 0.5059 0.468 −17%  531 3-hydroxy-3- 50 0.3917 0.4342 24% methylglutarate 541 4-hydroxyphenylacetate 50 0.8452 0.5813  6% 542 3-hydroxybutanoic acid 50 0.2058 0.3499 −59%  554 adenine 50 0.2549 0.3783 25% 555 adenosine 35 0.0081 0.3021 81% 563 alpha-L-sorbopyranose 50 0.1978 0.3499 94% 569 caffeine 35 0.029 0.3021 −53%  575 arabinose 50 0.0415 0.3021 72% 577 fructose 50 0.8863 0.591  7% 581 glucose 50 0.061 0.3283 52% 587 gluconic acid 50 0.1217 0.3401 64% 594 niacinamide 35 0.5535 0.4815 −9% 597 phosphoenolpyruvate 35 0.3699 0.4225 50% 605 uracil 50 0.1162 0.3401 35% 607 urocanic acid 35 0.7279 0.5417 11% 608 vitamin-B6 35 0.7072 0.5385 −15%  1101 3-methoxy-4- 50 0.0137 0.3021 55% hydroxyphenylacetate 1107 allantoin 50 0.0607 0.3283 73% 1125 isoleucine 50 0.1449 0.3451 32% 1126 alanine 50 0.2233 0.3499 35% 1284 threonine 50 0.094 0.3377 38% 1299 tyrosine 50 0.0817 0.3308 39% 1302 methionine 35 0.0101 0.3021 54% 1303 malic acid 35 0.627 0.5038 11% 1366 trans-4-hydroxyproline 50 0.7014 0.5382  9% 1413 3-hydroxyphenylacetate 35 0.5846 0.4923 17% 1417 kynurenic acid 50 0.1657 0.3499 68% 1418 5-6-Dihydrothymine 35 0.075 0.3283 46% 1419 5-s-methyl-5- 35 0.3383 0.411 17% thioadenosine 1431 (p-Hydroxyphenyl)lactic 50 0.4665 0.4614 42% acid 1432 alphahydroxybenzeneacetic 35 0.7224 0.5417  6% acid 1437 succinate 50 0.6047 0.497 13% 1444 DL-pipecolic acid 35 0.749 0.5497 18% 1480 guanidineacetic acid 35 0.0242 0.3021 99% 1493 ornithine 50 0.9781 0.6148 −1% 1494 5-oxoproline 50 0.3008 0.4095 24% 1498 N-6-trimethyl-l-lysine 35 0.1516 0.3464 37% 1505 orotic acid 50 0.2192 0.3499 38% 1508 pantothenic acid 35 0.3229 0.411 57% 1519 sucrose 50 0.9951 0.6181  0% 1557 3-methylglutaric acid 35 0.5659 0.4828 −11%  1558 4-acetamidobutyric acid 35 0.026 0.3021 60% 1559 5-6-dihydrouracil 50 0.1436 0.3451 26% 1560 L-methyldopa 35 0.597 0.497 14% 1564 citric acid 50 0.1013 0.3377 44% 1566 3-amino-isobutyrate 50 0.6838 0.5331 16% 1567 4-hydroxy-3- 50 0.0688 0.3283 53% methoxymandelate 1568 4-hydroxymandelate 50 0.2224 0.3499 45% 1569 DL-beta- 35 0.4431 0.4524 17% hydroxyphenylethylamine 1574 histamine 35 0.0188 0.3021 61% 1580 noradrenaline 50 0.2067 0.3499 50% 1585 N-acetyl-L-alanine 35 0.5273 0.4762 14% 1587 N-acetyl-L-leucine 35 0.1465 0.3451 41% 1591 N-acetyl-L-valine 35 0.2387 0.3625 50% 1592 N-acetylneuraminic acid 50 0.0338 0.3021 48% 1598 N-tigloylglycine 35 0.1685 0.3499 29% 1604 uric acid 35 0.3873 0.4336  5% 1640 ascorbic acid 50 0.6695 0.5267 54% 1648 serine 50 0.0526 0.3216 45% 1649 valine 50 0.1421 0.3451 26% 1708 7-8-dihydrofolic acid 35 0.1975 0.3499 23% 1778 gamma-glu-cys 35 0.4675 0.4614 47% 1827 riboflavine 35 0.2142 0.3499 183%  1860 3-nitro-L-tyrosine 35 0.974 0.6137 −1% 1868 cysteine 50 0.6881 0.534 23% 1898 proline 50 0.2767 0.395 46% 1899 quinolinic acid 50 0.1071 0.3377 40% 2078 pyrophosphate 50 0.3706 0.4225 44% 2092 catechol 35 0.018 0.3021 145%  2132 citrulline 50 0.0991 0.3377 35% 2183 thymidine 35 0.8751 0.5877  3% 2245 Metabolite - 294 35 0.3345 0.411 43% 2342 serotonin 35 0.0762 0.3283 34% 2734 gamma-L-glutamyl-L- 35 0.0437 0.3021 66% tyrosine 2829 N-formyl-L-methionine 35 0.4526 0.4591 23% 2831 adenosine-3-5-cyclic- 35 0.008 0.3021 43% monophosphate 3127 hypoxanthine 35 0.2239 0.3499 51% 3138 pyridoxamine-phosphate 35 0.8095 0.5733  5% 3147 xanthine 35 0.207 0.3499 60% 3155 3-ureidopropionic acid 35 0.1334 0.3451 67% 4966 xylitol 50 0.2152 0.3499 −31%  5493 Metabolite - 1059 35 0.8769 0.5877 −3% 5495 Metabolite - 1060 35 0.8529 0.5835 −2% 5514 Metabolite - 1081 35 0.026 0.3021 11% 5538 Metabolite - 1101 35 0.471 0.4614 59% 5664 Metabolite - 1101 35 0.6342 0.505 −15%  5687 Metabolite - 1110 35 0.306 0.411 23% 5697 acetylcarnitine- 35 0.8627 0.5857 −6% 5702 Metabolite - 1114 35 0.2517 0.3783 65% 5711 2-hydroxybutyric acid 35 0.7942 0.5658 −5% 5719 Metabolite - 1122 35 0.4991 0.4678 12% 5727 Metabolite - 1126 35 0.9422 0.6067 −2% 5797 Metabolite - 1186 35 0.2577 0.3783 30% 6137 Metabolite - 1212 35 0.1231 0.3401 36% 6147 Metabolite - 1216 35 0.5353 0.4784 −9% 6238 normetanephrine 50 0.2336 0.3587 17% 6253 Metabolite - 1283 35 0.4722 0.4614 35% 6278 Metabolite - 1289 35 0.4075 0.4428 13% 6329 urea 50 0.2047 0.3499 24% 6362 Metabolite - 1323- 35 0.1689 0.3499 56% possible-p-cresol-sulfate 6398 Metabolite - 1335 35 0.4993 0.4678 12% 6405 Metabolite - 1338 35 0.4624 0.4614 −16%  6413 Metabolite - 1342- 35 0.1592 0.3499 28% possible- phenylacetylglutamine-or- formyl-N-acetyl-5- methoxykynurenamine 6421 Metabolite - 1345 35 0.2195 0.3499 71% 6437 Metabolite - 1349- 35 0.1055 0.3377 40% possible-N-acetyl-8-O- methyl-Neuraminic acid 6443 Metabolite - 1351 35 0.9453 0.6072  2% 6477 Metabolite - 1364 35 0.3548 0.419 −65%  6486 Metabolite - 1368 35 0.4304 0.4492 45% 6493 salicyluric acid 35 0.462 0.4614 −65%  6528 Metabolite - 1383- 35 0.4273 0.4492 −49%  possible-salicyluric- glucuronide 6760 Metabolite - 1455 35 0.0092 0.3021 58% 6764 Metabolite - 1459 35 0.7767 0.5619 13% 6777 Metabolite - 1463 35 0.5723 0.485 −31%  6787 Metabolite - 1465 35 0.2726 0.3914 20% 6847 Metabolite - 1496 35 0.7359 0.5447 15% 6852 Metabolite - 1498 35 0.4113 0.4428 49% 6987 Metabolite - 1573 35 0.2236 0.3499 16% 7132 Metabolite - 1667 35 0.3277 0.411 −73%  7175 Metabolite - 1655 35 0.1087 0.3377 77% 7177 Metabolite - 1656 35 0.5561 0.4815 23% 7272 Metabolite - 1679 35 0.1129 0.3377 42% 7286 Metabolite - 1682 35 0.8923 0.5935  4% 7359 n-acetyl-L-aspartic acid 35 0.0709 0.3283 67% 7639 oxalic acid 35 0.1367 0.3451 25% 7650 Metabolite - 1834 35 0.4803 0.464 −21%  7660 Metabolite - 1839 35 0.9276 0.6009 −3% 7672 Metabolite - 1843 35 0.1049 0.3377 30% 7933 Metabolite - 1911 35 0.2835 0.4005 42% 8176 Metabolite - 1974 35 0.1493 0.3451 43% 8196 Metabolite - 1979-Cl- 35 0.7488 0.5497 −10%  adduct-of-isobar-19 8210 Metabolite - 1981 35 0.9643 0.6134  1% 8336 Metabolite - 2005 35 0.6224 0.5032 −11%  8644 Metabolite - 2051 35 0.5497 0.4815  8% 8677 Metabolite - 2056 35 0.1235 0.3401 24% 9007 Metabolite - 2108 35 0.6911 0.534 17% 9038 Metabolite - 2118 35 0.2102 0.3499 29% 9113 Metabolite - 2133 35 0.2101 0.3499 32% 9165 Metabolite - 2150 35 0.3592 0.4194 29% 9333 Metabolite - 2174 35 0.514 0.4716 15% 9334 Metabolite - 2175 35 0.3253 0.411 73% 9458 Metabolite - 2181 35 0.0095 0.3021 56% 10058 Metabolite - 2242 35 0.3061 0.411 −91%  10087 Metabolite - 2249 35 0.2194 0.3499 79% 10122 Metabolite - 2254 35 0.9247 0.6009 −2% 10136 Metabolite - 2034 35 0.3572 0.419 42% 10156 Metabolite - 2259 35 0.331 0.411 85% 10240 4-acetominophen-sulfate 35 0.6091 0.497 15% 10245 Metabolite - 2269- 35 0.715 0.5393  5% 10247 Metabolite - 2270 35 0.135 0.3451 −49%  10252 Metabolite - 2271 35 0.2042 0.3499 38% 10286 Metabolite - 2272 35 0.1989 0.3499 80% 10309 Metabolite - 2277 35 0.0163 0.3021 59% 10347 Metabolite - 2285 35 0.1474 0.3451 44% 10407 Metabolite - 2059 35 0.3262 0.411 22% 10424 Metabolite - 2292 35 0.0485 0.3188 −36%  10433 Metabolite - 2293- 35 0.3264 0.411 −91%  possible-O- desmethylvenlafaxine- glucuronide 10490 Metabolite - 2319 35 0.6803 0.5319  8% 10526 Metabolite - 2323 35 0.8122 0.5733  7% 10544 Metabolite - 2329 35 0.5016 0.4678 11% 10555 Metabolite - 2348 35 0.3735 0.4225 −36%  10570 Metabolite - 2366 35 0.5627 0.4824 13% 10629 Metabolite - 2386 35 0.2987 0.4088 19% 10644 Metabolite - 2387 35 0.374 0.4225 39% 10667 Metabolite - 2389 35 0.207 0.3499 −22%  10672 Metabolite - 2390 35 0.0744 0.3283 69% 10737 Isobar-1-includes- 35 0.9075 0.6009 −4% mannose-fructose- glucose-galactose-alpha- L-sorbopyranose-Inositol- D-allose-D--altrose-D- psicone 10741 Isobar-2-includes-2- 35 0.7125 0.5393 −9% aminoisobutyric acid-3- amino-isobutyrate-2- amino-butyrate-4- aminobutanoic acid- dimethylglycine-choline- 10743 Isobar-4-includes- 35 0.0298 0.3021 48% Gluconic acid-DL- arabinose-D-ribose-L- xylose-DL-lyxose-D- xylulose 10746 Isobar-6-includes-valine- 35 0.5275 0.4762 14% betaine 10785 Metabolite - 2506 35 0.886 0.591  3% 10825 Metabolite - 2546 35 0.3726 0.4225 15% 10872 Metabolite - 2550 35 0.1131 0.3377 100%  10906 Metabolite - 2557- 35 0.7733 0.5619 −8% possible-Pantoprazole- metabolite 11053 Metabolite - 2567 35 0.0376 0.3021 42% 11085 Metabolite - 2588 35 0.3891 0.4336 64% 11110 Metabolite - 2591 35 0.0842 0.3353 −35%  11173 Metabolite - 2607 35 0.7772 0.5619 12% 11219 Metabolite - 2686 35 0.818 0.5733  5% 11264 Metabolite - 2698 35 0.2618 0.38 −43%  11271 Metabolite - 2700 35 0.4829 0.4648 29% 11292 Metabolite - 2703 35 0.0818 0.3308 25% 11299 Metabolite - 2706 35 0.4803 0.464 21% 11390 Metabolite - 2726 35 0.1731 0.3499 31% 11411 Metabolite - 2746 35 0.6339 0.505 −9% 11438 phosphate 50 0.2251 0.3499 27% 11484 Metabolite - 2752 35 0.077 0.3283 35% 11661 Metabolite - 2781 35 0.0671 0.3283 30% 11777 glycine 50 0.0787 0.3283 44% 11808 Metabolite - 2807 35 0.9564 0.6099  2% 11851 Metabolite - 2811 35 0.497 0.4678 28% 12025 cis-aconitic acid 50 0.8152 0.5733 12% 12055 galactose 50 0.9486 0.6079  3% 12102 o-phosphoethanolamine 50 0.4724 0.4614 25% 12104 Metabolite - 2852 35 0.2242 0.3499 199%  12109 Metabolite - 2853 35 0.6302 0.5048 14% 12129 beta-hydroxyisovaleric 50 0.0907 0.3377 43% acid 12300 Metabolite - 2868 35 0.3468 0.4142 109%  12358 (1′R,1′S)_biopterin 35 0.8705 0.5877  3% 12426 Metabolite - 2416 35 0.329 0.411 44% 12463 Metabolite - 2893- 35 0.8121 0.5733  7% possible-demethylated- Rosiglitazone 12474 Metabolite - 2897 35 0.5015 0.4678 16% 12593 Metabolite - 2973 50 0.8428 0.5812  3% 12641 meso-erythritol 50 0.2553 0.3783 30% 12644 Metabolite - 3016 50 0.7026 0.5382  5% 12648 Metabolite - 3020 50 0.0318 0.3021 62% 12666 Metabolite - 3033 50 0.0908 0.3377 47% 12711 Metabolite - 3053 35 0.8572 0.5835 −8% 12720 Metabolite - 3056 35 0.1203 0.3401 30% 12765 inositol 50 0.986 0.6153 −1% 12770 Metabolite - 3090 50 0.0682 0.3283 25% 12771 Metabolite - 3091 50 0.1481 0.3451 83% 12795 Metabolite - 3113 50 0.9736 0.6137 −1% 12856 Metabolite - 3123 35 0.7353 0.5447 −8% 12902 Metabolite - 3127 35 0.5559 0.4815 14% 12904 Metabolite - 2457 35 0.7826 0.5634 10% 12924 Metabolite - 3131 35 0.3436 0.4141 31% 12938 Metabolite - 2459 35 0.662 0.5223 11% 13018 Metabolite - 3138 35 0.6504 0.5163 12% 13136 Metabolite - 3163- 35 0.3216 0.411 17% possible-methylcytidine- benserazide-Pyr-Gln-OH- or- glycerophosphocholine- 13153 Metabolite - 3169 35 0.264 0.3811 132%  13179 Metabolite - 3176 35 0.4698 0.4614 30% 13214 Metabolite - 3183- 35 0.1413 0.3451 45% possible-gamma-L- glutamyl-L-phenylalanine 13217 Metabolite - 3184 35 0.1112 0.3377 37% 13249 Metabolite - 3215 35 0.0274 0.3021 40% 13251 Metabolite - 3216 35 0.0408 0.3021 17% 13265 Metabolite - 3221 35 0.2155 0.3499 34% 13297 Metabolite - 3231 35 0.7164 0.5393  6% 13318 DL-indole-3-lactic acid 35 0.7541 0.5513 12% 13356 Metabolite - 3246- 35 0.0524 0.3216 76% possible-Ala-GLy-glycyl- sarcosine-or-ureido- butyric acid 13459 Metabolite - 3305 35 0.1936 0.3499 47% 13484 Metabolite - 3309 35 0.491 0.4678 13% 13493 Metabolite - 3311- 35 0.1657 0.3499 −40%  13505 Metabolite - 3313 35 0.6134 0.4974 17% 13534 Metabolite - 3320- 35 0.559 0.4815 34% possible-pimpinellin-or- tetrahydroxybenzophenone 13545 Metabolite - 3322 35 0.0622 0.3283 90% 13589 Metabolite - 3327 35 0.2577 0.3783 37% 13594 Metabolite - 3329 35 0.0616 0.3283 149%  13704 Metabolite - 3355 35 0.6078 0.497 11% 13744 Metabolite - 3364 35 0.2939 0.4065 −19%  13775 Metabolite - 3370 35 0.1617 0.3499 35% 13791 Metabolite - 3373 35 0.3232 0.411 1137%  13803 Metabolite - 3377 35 0.4791 0.464 37% 13817 Metabolite - 3380 35 0.094 0.3377 53% 13820 beta-nicotinamide- 35 0.0454 0.3059 45% mononucleotide 13847 Metabolite - 3387 35 0.3384 0.411 23% 13904 Metabolite - 3402 35 0.3627 0.4198 −38%  13968 Metabolite - 3409 35 0.9107 0.6009  4% 14036 Metabolite - 3427 35 0.1123 0.3377 58% 14066 Metabolite - 3433 35 0.4093 0.4428 12% 14084 Metabolite - 3436 35 0.8736 0.5877  2% 14115 Metabolite - 3440 35 0.8294 0.575 −5% 14125 Metabolite - 3443 35 0.0154 0.3021 −54%  14170 Metabolite - 3457 35 0.6072 0.497 −11%  14220 Metabolite - 3470 35 0.0626 0.3283 84% 14249 Metabolite - 3476 35 0.7481 0.5497 −9% 14368 Metabolite - 3489 35 0.1826 0.3499 −28%  14406 Metabolite - 3493 35 0.4363 0.4492 17% 14453 Metabolite - 3507 35 0.8289 0.575 −5% 14471 Metabolite - 3516 35 0.3193 0.411 −21%  14506 Metabolite - 3543 35 0.3275 0.411 111%  14539 Metabolite - 3564 35 0.2138 0.3499 107%  14595 Metabolite - 3576 35 0.1745 0.3499 63% 14640 Metabolite - 3604 35 0.9887 0.6156  0% 14641 Metabolite - 3605 35 0.5637 0.4824 24% 14731 Metabolite - 3659 35 0.5691 0.4839 14% 14732 Metabolite - 3660 35 0.627 0.5038 25% 14733 Metabolite - 3661 35 0.4336 0.4492 28% 14759 Metabolite - 3667 35 0.828 0.575  4% 14762 Metabolite - 3668 35 0.7262 0.5417 −6% 14766 Metabolite - 3670 35 0.8506 0.5835 −4% 14769 Metabolite - 3691 35 0.0162 0.3021 135%  14808 Metabolite - 3 701 35 0.1711 0.3499 61% 14835 Metabolite - 3706 35 0.353 0.419 19% 14840 Metabolite - 3708 35 0.202 0.3499 184%  14907 Metabolite - 3734 35 0.1065 0.3377 49% 14983 Metabolite - 3754 35 0.9841 0.6153 −1% 14984 Metabolite - 3755 35 0.3568 0.419 −60%  15017 Metabolite - 3761 35 0.3351 0.411 15% 15057 Metabolite - 3771 35 0.7946 0.5658 −9% 15064 Metabolite - 3773 35 0.4963 0.4678 17% 15096 N-acetyl-D-glucosamine 50 0.0305 0.3021 81% 15121 Metabolite - 3786 35 0.9216 0.6009 −3% 15124 porphobilinogen 35 0.3291 0.411 22% 15125 (2- 35 0.0419 0.3021 25% Aminoethyl)phosphonate 15128 DL-homocysteine 35 0.4331 0.4492 20% 15129 D-alanyl-D-alanine 35 0.2018 0.3499 27% 15130 diaminopimelic acid 35 0.0787 0.3283 49% 15131 dethiobiotin 35 0.4377 0.4492 74% 15187 Metabolite - 3800 35 0.1005 0.3377 82% 15197 Metabolite - 3802 35 0.3395 0.411 40% 15201 Metabolite - 3803 35 0.1397 0.3451 45% 15202 Metabolite - 3804 35 0.0505 0.3216 62% 15203 Metabolite - 3805 35 0.1828 0.3499 32% 15207 Metabolite - 3806 35 0.1977 0.3499 95% 15211 Metabolite - 3807 35 0.0352 0.3021 30% 15220 Metabolite - 3813 35 0.3153 0.411 26% 15228 Metabolite - 3817 35 0.0645 0.3283 62% 15240 Metabolite - 3824 35 0.837 0.5787  8% 15249 Metabolite - 3828 35 0.176 0.3499 43% 15251 Metabolite - 3830 35 0.5535 0.4815 20% 15253 Metabolite - 3832- 35 0.9729 0.6137  1% possible-phenol-sulfate 15258 Metabolite - 3834-Peptide 35 0.5236 0.476 27% 15275 Metabolite - 3840 35 0.5876 0.4932 12% 15276 Metabolite - 3841 35 0.7884 0.5652 −12%  15278 Metabolite - 3843 35 0.1684 0.3499 48% 15284 Metabolite - 3847 35 0.9363 0.6044  2% 15294 Metabolite - 3855 35 0.9278 0.6009 −2% 15312 Metabolite - 3873 35 0.1113 0.3377 41% 15315 Metabolite - 3876 35 0.6801 0.5319 −17%  15324 Metabolite - 3878 35 0.9543 0.6099 −1% 15326 Metabolite - 3879 35 0.5214 0.4756 40% 15328 azelaic acid 35 0.5573 0.4815 27% 15335 mannitol 50 0.7785 0.5619 10% 15336 tartaric acid 35 0.1794 0.3499 69% 15356 Metabolite - 3886 35 0.3988 0.4403 17% 15359 Metabolite - 3887 35 0.8182 0.5733  6% 15365 sn-Glycerol-3-phosphate 50 0.5348 0.4784 23% 15374 Metabolite - 3893 35 0.4706 0.4614 15% 15382 Metabolite - 3898 35 0.287 0.4018 14% 15410 Metabolite - 3908 35 0.1311 0.3451 50% 15411 Metabolite - 3909 35 0.559 0.4815 −17%  15418 Metabolite - 3911 35 0.6533 0.517  7% 15496 agmatine 35 0.8254 0.575 −7% 15500 carnitine 35 0.191 0.3499 −31%  15529 Metabolite - 3951 35 0.0572 0.3283 34% 15532 Metabolite - 3952 35 0.3275 0.411 49% 15535 Metabolite - 3955 35 0.0891 0.3377 71% 15541 Metabolite - 3957 35 0.7242 0.5417  5% 15599 Metabolite - 3963 35 0.5152 0.4716 72% 15610 Metabolite - 3970 35 0.3254 0.411 24% 15620 Metabolite - 3973 35 0.2969 0.4084 94% 15626 Metabolite - 3977 35 0.2082 0.3499 65% 15636 Metabolite - 3981 35 0.3746 0.4225 49% 15641 Metabolite - 3986 35 0.6892 0.534  9% 15650 1-methyladenosine 35 0.0431 0.3021 54% 15667 2-isopropylmalic acid 50 0.9286 0.6009 −3% 15676 3-methyl-2-oxovaleric 35 0.7554 0.5513 −9% acid 15677 3-methyl-L-histidine 35 0.0247 0.3021 50% 15679 xanthurenic acid 50 0.428 0.4492 25% 15681 4-Guanidinobutanoic acid 35 0.1785 0.3499 33% 15704 heptanedioic acid 35 0.0437 0.3021 42% 15716 L-beta-imidazolelactic 50 0.4297 0.4492 31% acid 15730 suberic acid 35 0.5965 0.497 13% 15737 hydroxyacetic acid 50 0.1005 0.3377 38% 15743 N-N-dimethylarginine 35 0.1977 0.3499 25% 15753 hippuric acid 35 0.6063 0.497 11% 15778 benzoic acid 35 0.2541 0.3783 −35%  15804 maltose 50 0.4101 0.4428 −43%  15835 L-xylose 50 0.4221 0.4492 47% 15948 S-adenosyl-l- 35 0.1118 0.3377 40% homocysteine 15964 D-arabitol 50 0.0578 0.3283 52% 16002 Metabolite - 3992- 35 0.5021 0.4678 −9% possible-Cl-adduct-of- Formate-dimer 16016 Metabolite - 3994 35 0.0348 0.3021 90% 16034 Metabolite - 4002 50 0.5041 0.468 17% 16071 Metabolite - 4020 50 0.0755 0.3283 44% 16082 Metabolite - 4027 50 0.1596 0.3499 33% 16107 lysine 50 0.6063 0.497 −30%  16175 Metabolite - 4092 35 0.4353 0.4492 22% 16197 Metabolite - 4112 35 0.122 0.3401 43% 16217 Metabolite - 4116 35 0.5789 0.4891 30% 16230 Isobar-29-includes-R-S- 35 0.2117 0.3499 135%  hydroorotic acid-5-6- dihydroorotic acid 16232 Isobar-17-includes- 35 0.4014 0.4413 −23%  arginine-N-alpha-acetyl- ornithine 16233 Isobar-13-includes-5-keto- 35 0.3895 0.4336 63% D-gluconic acid-2-keto-L- gulonic acid-D-glucuronic acid-D-galacturonic acid 16235 Isobar-19-includes-D- 35 0.5538 0.4815 22% saccharic acid-1-5- anhydro-D-glucitol-2- deoxy-D-galactose-2- deoxy-D-glucose-L- fucose-L-rhamnose 16243 L-kynurenine 35 0.2602 0.3798 353%  16276 Isobar-38-includes-N- 35 0.3469 0.4142 −19%  acetyl-L-methionine-5- hydroxy-1H-indole-3- acetic acid 16278 Isobar-35-includes-D- 35 0.874 0.5877 −3% arabinose-5-phosphate-D- ribulose-5-phosphate- alpha-D-ribose-5- phosphate 16290 Metabolite - 4133 50 0.8566 0.5835 −7% 16337 Metabolite - 4167 35 0.9854 0.6153 −1% 16338 Metabolite - 4168 35 0.2875 0.4018 183%  16457 Metabolite - 4233 35 0.0702 0.3283 115%  16462 Metabolite - 4234 35 0.7005 0.5382 30% 16496 Metabolite - 4251 50 0.0239 0.3021 99% 16506 Metabolite - 4271 50 0.7894 0.5652  9% 16816 Metabolite - 4494 50 0.4086 0.4428 −7% 16818 Metabolite - 4495 50 0.7053 0.5385  8% 16819 Metabolite - 4496 50 0.4299 0.4492 10% 16821 Metabolite - 4498 50 0.9186 0.6009  3% 16822 Metabolite - 4499 50 0.132 0.3451 42% 16823 Metabolite - 4500 50 0.5079 0.4682 59% 16824 iminodiacetic acid 50 0.1695 0.3499 60% 16827 Metabolite - 4502 50 0.0356 0.3021  6% 16829 Metabolite - 4503 50 0.1744 0.3499 30% 16831 Metabolite - 4504 50 0.0255 0.3021 47% 16834 Metabolite - 4505 50 0.1844 0.3499 68% 16837 Metabolite - 4507 50 0.9737 0.6137  1% 16848 Metabolite - 4511 50 0.5473 0.4815 22% 16851 Metabolite - 4512 50 0.5346 0.4784 −25%  16859 Metabolite - 4516 50 0.1479 0.3451 95% 16860 Metabolite - 4517 50 0.9134 0.6009 −3% 16861 Metabolite - 4518 50 0.5481 0.4815 45% 16862 Metabolite - 4519 50 0.0417 0.3021 147%  16863 Metabolite - 4520 50 0.49 0.4678 −23%  16864 Metabolite - 4521 50 0.5999 0.497 −17%  16865 Metabolite - 4522 50 0.0317 0.3021 45% 16866 Metabolite - 4523 50 0.1397 0.3451 35% 16867 Metabolite - 4524 50 0.4657 0.4614 41% 16952 Metabolite - 4593 50 0.1068 0.3377 32% 16959 Metabolite - 4595 50 0.206 0.3499 26% 17028 Metabolite - 4611 50 0.191 0.3499 30% 17050 Metabolite - 4618 50 0.7146 0.5393 −14%  17064 Metabolite - 4624 50 0.4442 0.4524 15% 17072 Metabolite - 4628 50 0.6112 0.4971 29% 17074 Metabolite - 4629 50 0.2339 0.3587 32% 17080 Metabolite - 4632 50 0.3386 0.411 64% 17083 Metabolite - 4634 50 0.2892 0.402 32% 17084 Metabolite - 4635 50 0.9283 0.6009 −6% 17085 Metabolite - 4636 50 0.1467 0.3451 50% 17086 Metabolite - 4637 50 0.1763 0.3499 99% 17087 Metabolite - 4638 50 0.4336 0.4492 37% 17088 Metabolite - 4639 50 0.2348 0.3587 58%

Example 5 Random Forest Analysis for the Classification of Tissue Samples

The data obtained in Example 1 concerning the tissue samples was used to create a random forest model. Random Forest Analysis was carried out on the data obtained from tissue samples in Example 1 to classify them as Normal (N), Localized (i.e. lower grade) cancer tumor (T) or Metastatic tumor (M). The first analysis resulted in 90.5% correct classification of the three tissue types. The metastatic tumors were correctly classified 100% of the time while the normal tissue and the localized prostate cancer tumors were correctly classified 87% and 83%, respectively (Table 12).

TABLE 12 Confusion matrix for metastatic (M), normal prostate (N) and localized prostate cancer tumor (T) tissue types. Predicted Tissue Type Metastatic Normal Localized Tumor Tissue Tumor error Actual Metastatic Tumor (M) 14 0 0 0.00 Tissue Normal Tissue (N) 0 14 2 0.13 Type Localized Tumor (T) 0 2 10 0.17 OOB Error = 9.5%

Based on the OOB Error rate of 9.5%, the Random Forest model that was created could be used to predict whether a subject has a metastatic tumor (M), a localized tumor (T), or normal tissue (N) with about 90.5% accuracy from analysis of the levels of the biomarkers in samples from the subject.

The importance plot is shown in FIG. 1. The important metabolites for this classification are listed in Table 13.

TABLE 13 Most important biomarker metabolites to distinguish N, T, M tissue types. Metabolite Library n-hexadecanoic acid (palmitate) 50 tetradecanoic acid (myristate) 50 inosine 61 octadecanoic acid 50 (stearate(3-amino-isobutyrate 50 n-dodecanoate (laurate) 50 (2-aminoethyl)phosphonate 61 Metabolite - 3778 61 glycerol 50 (p-hydroxyphenyl)lactic acid 50 palmitoleic acid 50 N-acetyl-D-galactosamine 50 Metabolite - 3102 50 meso-erythritol 50 Metabolite - 1597 61 uracil 50 uridine 61 Metabolite - 4075 50 Isobar-24 includes L-arabitol & adonitol 61 Metabolite - 2867 61 Metabolite - 4620 61 sn-glycerol-3-phosphate 50 Metabolite - 4117 (possible-propranolol or 2- 61 heptyl-3-hydroxy-quinolone Metabolite - 1114 61 DL-homocysteine 61 leucine 50 xanthine 61 Metabolite - 1576 61 Metabolite - 2973 50 Metabolite - 3810 61

Based on this analysis one sample appeared to be an outlier. Sample T3, which was reported as localized prostate tumor tissue, appears to be an outlier. From the random forest comparing all three tissue types, 80% of the trees in the random forest classified T3 as an “N” or normal while only 17% correctly classed it as “T” and only 3% classified it as “M” or metastatic. This result indicates that the sample may be a mixture of normal and cancerous tissue or that the sample is at an early stage of cancer.

Random Forest Analysis was also carried out on the tissue samples from the prostate to classify them as Normal prostate (N) or Localized prostate cancer tumor (T). This analysis resulted in 86% correct classification of the two tissue types. The normal tissue and the localized prostate cancer tumors were correctly classified 87% and 83% respectively (Table 14).

TABLE 14 Confusion matrix comparing Normal prostate tissue (N) with Localized prostate tumor tissue (T). Predicted Tissue Type Normal Localized Tissue Tumor error Actual Normal Tissue (N) 14 2 0.13 Tissue Localized Tumor 2 10 0.17 Type (T) OOB Error = 14%

Based on the OOB Error rate of 14%, the Random Forest model that was created could be used to predict whether a subject has normal tissue (N), or localized tumor (T) tissue with about 86% accuracy from analysis of the levels of biomarkers in samples from the subject.

The important metabolites for this classification are listed in Table 15 and shown in the importance plot in FIG. 2.

TABLE 15 Most important biomarker metabolites to distinguish Normal prostate tissue (N) and Cancerous prostate tumor tissue (T). Metabolite Library N-acetyl-D-galactosamine 50 Metabolite - 3778 61 uridine 61 Metabolite - 4117 possible propanolol or 2- 61 heptyl-3-hydroxy-quinolone Metabolite - 1114 61 Metabolite - 3810 61 DL-homocysteine 61 Metabolite - 3094 50 Metabolite - 4616 61 Metabolite - 1576 61 Metabolite - 3139 61 sn-glycerol-3-phospate 50 Metabolite - 2973 50 carnitine 61 Metabolite - 2041 61 Isobar 3 includes inositol-1-phosphate, 61 mannose-6-phospate, glucose-6-phosphate, D- mannose-1-phoaphate, alpha-D-glucose-1- phosphate kynurenic acid 61 n-hexadecanoic acid (palmitate) 50 cysteine 50 Metabolite - 4027 50 Metabolite - 3027 50 glutamic acid 50 Metabolite - 2688 61 Metabolite - 1111 possible 61 methylnitronitrosoguanidine or ethyl- thiocarbamoylacetate Metabolite - 2055 61 Metabolite - 3176 possible creatine 61 uracil 50 glycine 50 Metabolite - 3165 61 Metabolite - 1595 possible glutathione- 61 metabolite

Since the metastatic tumors were obtained from sites distal to the prostate, we determined if these tissues were distinguished from the normal and cancerous prostate tissue due to the metastasis or due to the location of the tumor. To test this, the metastatic tumor tissue samples from liver were compared with the non-liver metastatic tumor tissues using Random Forest. The confusion matrix resulting from this analysis is provided in Table 16 and the results are essentially random chance; the liver and non-liver origins of the tumors appear to be indistinguishable. Thus, the classification of the metastatic tumor tissue is based on the metabolite biomarkers for metastasis and not on the source tissue of the metastatic tumor.

TABLE 16 Confusion matrix comparing metastatic tumors from liver tissue with metastatic tumors from non-liver tissue. Predicted Tissue Source Liver Non-liver error Actual Liver 5 3 0.38 Tissue Non- 5 5 0.50 Source liver OOB Error = 43%

Based on the OOB Error rate of 43%, the Random Forest model that was created could be used to predict whether a subject has a metastatic tumor from liver tissue, compared to non-liver tissue, with about 57% accuracy from analysis of the levels of biomarkers in liver tissue samples from the subject, but the Error Rate may essentially be random chance and may indicate that the source (i.e. liver or non-liver) of the tumor tissue is not predicted by these biomarkers.

Example 6 Random Forest Analysis for the Classification of Urine Samples from Control Subjects, Subjects with Low Grade PCA and Subjects with High Grade PCA

Random Forest Analysis was carried out on the data obtained from urine samples in Example 4 to classify them as Non-cancer (Control) or Prostate cancer. The control samples were urine obtained from subjects with a Gleason score (major) of 0 or from prostate cancer (PCA) subjects with a Gleason score (major)>=4. The analysis resulted in 63% correct classification of the urine sample types. The control subjects were correctly classified 62% of the time while the subjects with prostate cancer (PCA) were correctly classified 64% of the time (Table 17).

TABLE 17 Confusion matrix for control vs PCA in Urine. Predicted Control PCA error Actual Control 33 20 0.38 PCA 5 9 0.36 OOB Error = 37%

Based on the OOB Error rate of 37%, the Random Forest model that was created could be used to predict whether a subject has prostate cancer (PCA), compared to being cancer-free, with about 63% accuracy from analysis of the levels of biomarkers in urine from the subject.

The importance plot is shown in FIG. 3. The important metabolites for this classification are listed in Table 18.

TABLE 18 Most important urine biomarker metabolites to distinguish Control from PCA. Metabolite Library (2-aminoethyl)phosphonate 35 Metabolite - 2051 35 Metabolite - 3805 35 Guanidineacetic acid 35 catechol 35 N-acetyl-L-valine 35 DL-beta-hydroxyphenylethylamine 35 N-acetyl-D-glucosamine 50 Metabolite - 1974 35 Metabolite - 4636 50 Metabolite - 2811 35 Metabolite - 3176 35 Metabolite - 1979 35 histamine 35 alpha-L-sorbopyranose 50 xylitol 50 Metabolite - 4504 50 Metabolite - 2285 35 3-methoxy-4-hydroxyphenylacetate 50 Metabolite - 2181 35 Metabolite - 1455 35 adenosine 35 Isobar 6: includes valine & betaine 35 Metabolite - 3169 35 Metabolite - 3470 35 Metabolite - 4502 50 Metabolite - 2746 35 Metabolite - 4503 50 Metabolite - 3476 35 Metabolite - 3443 35

Random Forest Analysis was also carried out on the biomarkers identified in Example 4 from urine samples to classify them as lower grade prostate cancer (Gleason score major 3) or higher grade prostate cancer (Gleason score major>=4). In this analysis resulted in 61% correct classification of the two cancer grades. The lower grade and the higher grade prostate cancers were correctly classified 58% and 71% respectively (Table 19).

TABLE 19 Confusion matrix comparing urine from subjects with lower grade prostate cancer and higher grade prostate cancer. Predicted Grade Low High error Actual Low 25 18 0.42 Grade High 4 10 0.29 OOB Error = 39%

Based on the OOB Error rate of 39%, the Random Forest model that was created could be used to predict whether a subject has a lower grade prostate cancer or a higher grade prostate cancer with about 61% accuracy from analysis of the levels of biomarkers in urine from the subject.

The importance plot is shown in FIG. 4. The important metabolites for this classification are listed in Table 20.

TABLE 20 Most important urine biomarker metabolites to distinguish subjects with lower grade prostate cancer and higher grade prostate cancer. Metabolite Library Metabolite - 3805 35 Histamine 35 Metabolite - 1455 35 Catechol 35 xylitol 50 Metabolite - 4636 50 Metabolite - 3691 35 Adenosine 35 Glycine 50 Metabolite - 3661 35 Metabolite - 3955 35 Methionine 35 Isobar 6 includes valine, betaine 35 guanidineacetic acid 35 Metabolite - 3817 35 N-acetyl-D-glucosamine 50 Metabolite - 3806 35 Metabolite - 2277 35 3-methoxy-4-hydroxyphenylacetate 50 Metabolite - 4502 50 Metabolite - 4519 50 Guanine 50 Metabolite - 2181 35 Alpha-L-sorbopyranose 50 Metabolite - 2270 35 Metabolite - 1498 35 Metabolite - 3443 35 tryptophan 35 Metabolite - 2051 35 gamma-L-glutamy-L-tyrosine 35

Example 7 Random Forest Analysis for the Classification of Plasma Samples from Control Subjects, Subjects with Lower Grade PCA and Subjects with Higher Grade PCA

Random Forest Analysis was carried out on data obtained in Example 3 from plasma samples to classify them as Non-cancer (Control) or Prostate cancer. The control samples were plasma obtained from subjects with a Gleason score (major) of 0 or from prostate cancer (PCA) subjects with a Gleason score (major) 3. The analysis resulted in 65% correct classification of the plasma sample types. The control subjects were correctly classified 68% of the time while the subjects with prostate cancer (PCA) were correctly classified 60% of the time (Table 21).

TABLE 21 Confusion matrix for Control vs Lower Grade PCA in Plasma. Predicted Control PCA error Actual Control 36 17 0.32 PCA 17 26 0.40 OOB Error = 35%

Based on the OOB Error rate of 35%, the Random Forest model that was created could be used to predict whether a subject has a lower grade prostate cancer or does not have prostate cancer with about 65% accuracy from analysis of the levels of biomarkers in plasma from the subject.

The importance plot is shown in FIG. 5. The important metabolites for this classification are listed in Table 22.

TABLE 22 Most important plasma biomarker metabolites to distinguish Control from Lower Grade PCA. Metabolite Library Metabolite - 1185 35 5-oxoproline 50 IHWESASLLR 35 Metabolite - 3765 35 Metabolite - 2753 35 Hydroxyproline form of bradykinin 35 D-alany-D-alanine 35 Metabolite - 5437 50 Metabolite - 2256 35 Metabolite - 5366 50 Metabolite - 4611 50 Alpha keto glutarate 35 Metabolite - 3167 35 Alpha-tocopherol 50 Metabolite - 3377 35 Metabolite - 1127 35 Iminodiacetic acid 50 Trans-4-hydroxyproline 50 Metabolite - 2111 35 Metabolite - 2853 35 Metabolite - 2185 35 Metabolite - 3030 50 Metabolite - 3017 50 Metabolite - 1083 35 N-acetyl-L-valine 35 Metabolite - 1597 35 Creatinine 35 Isobar 36 includes D-sorbitol-6-phosphate, 35 mannitol-1-phosphate Metabolite - 2914 50 Pyridoxal-phosphate 35

Random Forest Analysis was also carried out on the biomarkers from plasma samples in Example 3 to classify them as control (Gleason score major 0) or higher grade prostate cancer (Gleason score major>=4, PCA). In this analysis resulted in 73% correct classification of the plasma sample types. The control and the higher grade prostate cancers were correctly classified 58% and 71% respectively (Table 23).

TABLE 23 Confusion matrix comparing plasma from subjects without prostate cancer (Control) and with higher grade prostate cancer (High PCA). Predicted High Control PCA error Actual Control 36 17 0.32 High PCA 2 13 0.07 OOB Error = 27%

Based on the OOB Error rate of 27%, the Random Forest model that was created could be used to predict whether a subject has a higher grade prostate cancer or does not have prostate cancer with about 63% accuracy from analysis of the levels of biomarkers in plasma from the subject.

The importance plot is shown in FIG. 6. The important biomarker metabolites for this classification are listed in Table 24.

TABLE 24 Most important plasma biomarker metabolites to distinguish subjects without prostate cancer from those with higher grade prostate cancer. Metabolite Library Metabolite - 1185 35 Metabolite - 3377 35 Metabolite - 2329 35 Metabolite - 3603 35 Metabolite - 1127 35 Metabolite - 3305 35 Metabolite - 2389 35 Metabolite - 3088 50 Trans-4-hydroxyproline 50 Metabolite - 1104 35 Isobar 17 includes arginine, N-alpha-acetyl- 35 ornithine Metabolite - 4769 50 Metabolite - 2141 35 Metabolite - 3030 50 Metabolite - 2867 35 Metabolite - 3707 35 Metabolite - 3098 50 Palmitoleic acid 50 Metabolite - 2711 35 Tetradecanoic acid 50 Metabolite - 2287 35 Metabolite - 2407 35 DL-indole-3-lactic acid 50 Metabolite - 1286 35 Heptadecanoic acid 50 Metabolite - 3033 50 5-oxoproline 50 Metabolite - 3576 35 Tartaric acid 35 Metabolite - 1831 possible Cl adduct of 35 citrulline

Random Forest Analysis was also carried out on the biomarkers from plasma samples to classify them as lower grade prostate cancer (Gleason score major 3) or higher grade prostate cancer (Gleason score major>=4). This analysis resulted in 67% correct classification of the two cancer grades. The lower grade and the higher grade prostate cancers were correctly classified 65% and 71% respectively (Table 25).

TABLE 25 Confusion matrix classifying plasma from subjects with lower grade prostate cancer and higher grade prostate cancer. Predicted Grade Low High error Actual Low 28 15 0.35 Grade High 4 10 0.29 OOB Error = 33%

Based on the OOB Error rate of 33%, the Random Forest model that was created could be used to predict whether a subject has a lower grade prostate cancer or a higher grade prostate cancer with about 67% accuracy from analysis of the levels of biomarkers in plasma from the subject.

The importance plot is shown in FIG. 7. The important metabolites for this classification are listed in Table 26.

TABLE 26 Most important plasma biomarker metabolites to distinguish subjects with lower grade prostate cancer and higher grade prostate cancer. Metabolite Library Metabolite - 2329 35 Heptadecanoic acid 50 Metabolite - 3088 50 Metabolite - 3322 35 Palmitoleic acid 50 Isobar 6 includes valine and betaine 35 Tetradecanoic acid 50 Pyridoxal-phosphate 35 4-methyl-2-oxopentanoate 50 Metabolite - 3534 35 Metabolite - 2711 35 Isobar 17 includes arginine, N-alpha-acetyl- 35 ornithine. Octadecanoic acid 50 Caffeine 35 Metabolite - 2389 35 Metabolite - 3377 35 Metabolite - 3305 35 Leucine 50 Metabolite - 2407 35 Metabolite - 3900 35 Metabolite - 3992 possible Cl adduct of formate 35 dimmer Metabolite - 3089 50 Trans-4-hydroxypyruvate 50 Metabolite - 5437 50 N-hexadecanoic acid 50 Paraxanthine 35 Metabolite - 3603 35 M2130 35 Oleic acid 50 Metabolite - 1286 35

Example 8 Analytical Characterization of Unnamed Biomarkers Compounds

Table 27 below includes analytical characteristics of each of the isobars and the unnamed metabolites listed in Tables 1-26 above. The table includes, for each listed Isobar and Metabolite, the retention time (RT), retention index (RI), mass, quant mass, and polarity obtained using the analytical methods described above. “Mass” refers to the mass of the C12 isotope of the parent ion used in quantification of the compound. The values for “Quant Mass” give an indication of the analytical method used for quantification: “Y” indicates GC-MS and “1” indicates LC-MS. “Polarity” indicates the polarity of the quantitative ion as being either positive (+) or negative (−).

TABLE 27 Analytical Characteristics of Isobars and Unnamed Metabolites. COMPOUND RT RI MASS QUANT_MASS Polarity Isobar 1 includes mannose, 1.45 1481.0 215 1 − fructose, glucose, galactose, alpha-L-sorbopyranose, Inositol, D-allose Isobar 13 includes 5-keto-D- 1.40 1530.0 193.1 1 − gluconic acid, 2-keto-L-gulonic acid, D-glucuronic acid Isobar 17 includes arginine, N- 1.49 1620.0 175.2 1 + alpha-acetyl-ornithine Isobar 18 includes D-fructose 1- 1.33 1475.0 259.1 1 − phosphate, beta-D-fructose 6- phosphate Isobar 19 includes D-saccharic 1.55 1700.0 209 1 − acid, 2-deoxy-D-galactose, 2- deoxy-D-glucose, L-fucose, L- rhamnose Isobar 2 includes 3-amino- 1.60 1671.0 104.1 1 + isobutyrate, 2-amino-butyrate, 4- aminobutanoic acid, dimethylglycine, choline Isobar 20 includes fumaric acid, 4.45 4800.0 160.9 1 − 3-methyl-2-oxobutanoate Isobar 21 includes gamma- 1.59 1620.0 263.1 1 + aminobutyryl-L-histidine, L- anserine Isobar 22 includes glutamic acid, 1.55 1635.0 148 1 + O-acetyl-L-serine Isobar 24 includes L-arabitol, 1.43 1545.0 153.1 1 + adonitol Isobar 25 includes L-gulono-1,4- 1.67 1615.0 222.9 1 − lactone, glucono-gamma-lactone Isobar 27 includes L-kynurenine, 8.23 8470.0 209.1 1 + alpha-2-diamino-gamma- oxobenzenebutanoic acid Isobar 29 includes R,S- 2.17 2095.0 157.1 1 − hydroorotic acid, 5,6- dihydroorotic acid Isobar 3 includes inositol 1- 1.45 1467.0 304.7 1 − phosphate, mannose 6- phosphate, glucose 6-phosphate, D-mannose 1-phosphate, alpha- D-glucose 1-phosphate, alpha-D- galactose 1 phosphate Isobar 30 includes maltotetraose, 1.67 1770.0 710.8 1 − stachyose Isobar 31 includes maltotriose, 1.64 1752.0 548.8 1 − melezitose Isobar 32 includes N-acetyl-D- 1.57 1685.0 222 1 + glucosamine, N-acetyl-D- mannosamine Isobar 36 includes D-sorbitol 6- 1.37 1470.0 261.1 1 − phosphate, mannitol-1-phosphate Isobar 38 includes N-acetyl-L- 9.12 9220.0 192 1 + methionine, 5-hydroxy-1H-indole- 3-acetic acid Isobar 4 includes Gluconic acid, 1.52 1587.0 195 1 − DL-arabinose, D-ribose, L-xylose, DL-lyxose, D-xylulose Isobar 40 includes Maltotetraose, 1.67 2282.0 710.8 1 − stachyose Isobar 5 includes asparagine, 1.50 1395.0 133.1 1 + ornithine Isobar 6 includes valine, betaine 2.13 2160.0 118.1 1 + Isobar 9 includes sucrose, beta- 1.60 1605.0 386.9 1 − D-lactose, D-trehalose, D- cellobiose, D-Maltose, palatinose, melibiose, alpha-D-lactose Metabolite - 1069-possible 12.55 14450.0 367.2 1 − dehydroepiandrosterone sulfate Metabolite - 1070 9.00 9169.0 378.3 1 + Metabolite - 1085-possible 15.82 15964.0 288.1 1 + isolobinine or 4-aminoestra- 1,3,5(10)-triene-3,17beta-diol Metabolite - 1086 4.56 4811.0 294.1 1 + Metabolite - 1088 13.12 14225.0 369.1 1 − Metabolite - 1104 2.43 2410.0 201 1 − Metabolite - 1110 11.66 11841.0 269.1 1 − Metabolite - 1111 - possible 2.69 2700.0 148.1 1 + methylnitronitrosoguanidine or ethyl thiocarbamoylacetate Metabolite - 1113 - possible 4.91 5290.0 204.2 1 + acetylcarnitine Metabolite - 1114 2.19 2198.0 104.1 1 + Metabolite - 1116 4.20 4780.0 103.4 1 − Metabolite - 1121 16.29 16429.0 303.3 1 + Metabolite - 1122 4.45 4701.0 233.1 1 + Metabolite - 1126 3.04 3188.0 175.1 1 + Metabolite - 1127 12.18 12369.0 363.1 1 + Metabolite - 1129 5.16 5419.0 260.1 1 + Metabolite - 1133 - retired Na 1.63 1636.0 315 1 + adduct of EDTA Metabolite - 1142 - possible 5- 8.54 8739.0 163 1 − hydroxypentanoate or beta- hydroxyisovaleric acid Metabolite - 1183 8.56 8765.0 365.8 1 + Metabolite - 1185 8.70 9150.0 506.8 1 + Metabolite - 1186 8.83 9000.0 529.6 1 + Metabolite - 1187 8.80 9017.0 559.9 1 + Metabolite - 1188 8.83 9017.0 619.9 1 + Metabolite - 1203 - possible 9.11 9288.0 510.2 1 + HXGXA Metabolite - 1208 15.33 15494.0 319.4 1 − Metabolite - 1211 - possible 9.90 9800.0 606.5 1 + IHWESASLLR Metabolite - 1215 8.96 9390.0 550.1 1 + Metabolite - 1216 1.60 1631.4 343.9 1 − Metabolite - 1220 15.24 15402.5 319.2 1 + Metabolite - 1244 15.28 15436.8 343.4 1 − Metabolite - 1248 - possible 8.06 8275.4 302.3 1 + avermectin aglycone Metabolite - 1283 9.04 9244.5 434.8 1 + Metabolite - 1286 14.41 14579.8 229 1 + Metabolite - 1288 2.11 2120.5 302 1 − Metabolite - 1289 8.96 9139.7 338.4 1 + Metabolite - 1303 9.01 9178.0 527.8 1 + Metabolite - 1323 - possible p- 9.31 10000.0 187 1 − cresol sulfate Metabolite - 1327 - possible 13.22 13300.0 585.4 1 + bilirubin Metabolite - 1329 2.69 2791.0 210.1 1 + Metabolite - 1330 10.67 11097.7 436.3 1 + Metabolite - 1333 3.05 3794.0 321.9 1 + Metabolite - 1335 8.74 9162.2 367.2 1 + Metabolite - 1338 10.76 11193.0 241.1 1 − Metabolite - 1342 - possible 9.04 9459.4 265.2 1 + phenylacetylglutamine or formyl- N-acetyl-5-methoxykynurenamine Metabolite - 1349 3.50 3876.0 323.9 1 + Metabolite - 1351 1.77 1936.5 177.9 1 + Metabolite - 1364 10.35 10765.1 397.2 1 + Metabolite - 1368 8.18 8607.4 184.1 1 + Metabolite - 1383 - possible 8.66 9077.9 370.1 1 − salicyluric glucuronide Metabolite - 1389 - possible 13.62 14111.3 425.3 1 − glucuronide form of X-1359 Metabolite - 1392 10.90 11350.3 415.2 1 + Metabolite - 1394 - possible 12.28 12752.0 423.2 1 + Losartan Metabolite - 1455 2.38 2350.0 131.1 1 + Metabolite - 1457 1.59 1675.0 188.2 1 + Metabolite - 1465 3.45 3600.0 162.1 1 + Metabolite - 1496 1.53 1562.0 133 1 − Metabolite - 1497 13.87 14031.5 332.2 1 + Metabolite - 1498 1.56 1650.0 143.1 1 − Metabolite - 1573 1.63 1669.0 170.9 1 − Metabolite - 1575 2.25 2243.5 219.1 1 + Metabolite - 1576 2.51 2530.0 247.1 1 + Metabolite - 1593 2.67 2690.0 395.9 1 − Metabolite - 1594 3.15 3325.0 263.1 1 + Metabolite - 1595 - possible 3.14 3400.0 290.1 1 + glutathione metabolite Metabolite - 1596 3.66 3902.0 185 1 − Metabolite - 1597 3.66 4100.0 265.9 1 + Metabolite - 1608 8.08 8253.0 348.1 1 − Metabolite - 1609 8.31 8529.0 378 1 + Metabolite - 1616 12.73 12910.3 331.2 1 + Metabolite - 1653 16.84 16977.0 454.3 1 + Metabolite - 1655 1.31 1374.0 107 1 + Metabolite - 1656 1.46 1509.0 154.9 1 − Metabolite - 1679 8.52 8705.8 283.1 1 − Metabolite - 1680 8.50 8681.0 851.1 1 + Metabolite - 1682 8.78 8961.0 339.1 1 − Metabolite - 1713 2.73 3050.0 174 1 − Metabolite - 1718 8.43 8390.0 457.9 1 + Metabolite - 1817 1.37 1552.3 252 1 + Metabolite - 1819 1.36 1539.6 244.8 1 − Metabolite - 1829 1.43 1600.0 135 1 − Metabolite - 1831 - possible Cl 1.46 1638.7 209.9 1 − adduct of citrulline Metabolite - 1834 1.64 1960.0 104 1 − Metabolite - 1835 1.86 1999.3 152.1 1 − Metabolite - 1836 2.10 2215.5 205.9 1 − Metabolite - 1911 11.42 11799.6 464.1 1 + Metabolite - 1914 10.35 10719.8 239.1 1 + Metabolite - 1915 14.37 14798.6 507.2 1 − Metabolite - 1963 13.15 13550.8 464.1 1 + Metabolite - 1970 12.88 13271.2 852.9 1 + Metabolite - 1974 5.93 6300.0 160.2 1 + Metabolite - 1975 5.95 6093.0 344 1 + Metabolite - 1977 3.56 4060.0 260.9 1 + Metabolite - 1979 - Cl adduct of 1.52 1690.3 199 1 − isobar 19 Metabolite - 1980 13.20 13250.0 391.1 1 + Metabolite - 1981 7.94 8150.0 158.1 1 + Metabolite - 1988 11.14 11515.0 190.1 1 + Metabolite - 2005 8.62 9048.0 232.1 1 + Metabolite - 2027 1.56 1729.3 184.1 1 + Metabolite - 2036 14.30 14300.0 616.3 1 + Metabolite - 2041 13.84 14198.1 246.3 1 + Metabolite - 2051 1.45 1634.0 309 1 + Metabolite - 2053 1.35 1482.3 324.9 1 − Metabolite - 2055 1.37 1502.0 269.9 1 + Metabolite - 2056 1.37 1499.0 165.1 1 − Metabolite - 2064 8.00 8312.0 193.2 1 + Metabolite - 2072 1.57 1736.1 273.7 1 − Metabolite - 2074 2.24 2380.9 280.1 1 + Metabolite - 2075 2.71 2728.0 134.1 1 + Metabolite - 2099 7.82 8135.9 469.2 1 + Metabolite - 2100 1.33 1532.9 499 1 + Metabolite - 2105 8.15 8442.0 433.6 1 + Metabolite - 2108 8.76 8800.0 277.1 1 + Metabolite - 2109 8.99 9266.0 321.1 1 + Metabolite - 2111 9.19 9442.3 365.1 1 + Metabolite - 2118 13.10 13367.8 547.1 1 + Metabolite - 2121 14.18 14467.4 561.2 1 + Metabolite - 2129 15.83 16363.2 526.3 1 + Metabolite - 2130 16.28 16625.5 792.4 1 + Metabolite - 2139 8.09 8500.0 218.1 1 + Metabolite - 2141 9.39 9605.0 409.1 1 + Metabolite - 2143 10.11 10327.0 585.1 1 + Metabolite - 2150 13.27 13616.5 466.1 1 + Metabolite - 2174 2.50 2569.0 250.1 1 + Metabolite - 2175 3.84 4148.4 144 1 + Metabolite - 2180 8.32 8663.0 490 1 + Metabolite - 2181 8.37 8715.5 298 1 + Metabolite - 2185 9.22 9499.4 246.2 1 + Metabolite - 2194 13.65 13961.3 544.2 1 + Metabolite - 2198 13.97 14284.8 530.1 1 + Metabolite - 2212 15.96 16271.0 478.2 1 + Metabolite - 2232 2.26 2318.0 754.8 1 − Metabolite - 2237 10.14 10039.0 453.1 1 + Metabolite - 2242 11.61 11926.0 254.3 1 + Metabolite - 2249 14.21 14570.9 267.2 1 − Metabolite - 2250 14.26 14668.4 286.3 1 + Metabolite - 2254 1.53 1687.6 217.2 1 + Metabolite - 2255 - 9.08 9394.0 539.1 1 + hydroxyproline form of bradykinin Metabolite - 2256 9.93 9867.0 460.8 1 + Metabolite - 2259 11.25 11586.0 383.2 1 − Metabolite - 2269 10.36 10727.0 255.1 1 − Metabolite - 2271 12.95 13348.8 413.2 1 − Metabolite - 2272 7.96 8377.0 189.1 1 − Metabolite - 2277 10.07 10457.0 201.1 1 − Metabolite - 2279 12.38 12781.0 490.1 1 + Metabolite - 2281 13.93 14325.1 505.2 1 − Metabolite - 2285 2.00 2146.0 699.6 1 − Metabolite - 2287 12.95 13335.6 502.8 1 + Metabolite - 2292 2.40 2900.0 343.9 1 − Metabolite - 2293 - possible O- 8.86 9084.0 440.1 1 + desmethylvenlafaxine glucuronide Metabolite - 2313 1.56 1685.6 352.9 1 − Metabolite - 2316 8.82 9163.6 100.1 1 + Metabolite - 2319 12.24 12626.0 367.2 1 − Metabolite - 2323 7.55 7796.0 188.9 1 − Metabolite - 2329 11.76 12177.6 541.2 1 − Metabolite - 2347 13.65 14091.0 450.1 1 + Metabolite - 2348 13.91 14293.5 448.3 1 + Metabolite - 2366 8.47 8870.2 271 1 + Metabolite - 2368 9.27 9615.5 573.2 1 − Metabolite - 2370 16.13 16561.2 476.4 1 − Metabolite - 2386 11.94 12320.3 539.2 1 − Metabolite - 2387 8.55 9050.0 182.1 1 − Metabolite - 2388 16.16 16900.0 259.1 1 − Metabolite - 2389 1.49 1641.5 314.9 1 − Metabolite - 2390 6.09 6410.0 517.4 1 + Metabolite - 2391 10.14 10485.7 159.1 1 + Metabolite - 2392 13.08 13460.4 379 1 − Metabolite - 2406 14.69 15063.0 274.4 1 + Metabolite - 2407 15.72 16127.6 637.3 1 + Metabolite - 2466 9.19 8760.0 624.8 1 + Metabolite - 2486 1.52 1667.0 635.7 1 − Metabolite - 2506 14.05 14437.5 624.4 1 − Metabolite - 2507 14.44 14843.0 481.4 1 − Metabolite - 2546 1.63 1747.3 129.1 1 + Metabolite - 2548 - possible Cl 5.97 6430.0 202.9 1 − adduct of uric acid Metabolite - 2550 - possible 11.09 11490.0 411.1 1 + Riluzole glucuronide Metabolite - 2557 - possible 11.79 11968.1 354.2 1 + Pantoprazole metabolite Metabolite - 2558 - possible N1- 8.14 8800.0 153.1 1 + methyl-2-pyridone-5-carboxamide and others Metabolite - 2567 7.79 8464.7 247.1 1 + Metabolite - 2591 9.99 10189.4 279.3 1 + Metabolite - 2592 10.59 10600.0 697.4 1 − Metabolite - 2607 10.01 10354.0 578.2 1 + Metabolite - 2686 1.40 1593.0 219 1 − Metabolite - 2688 1.42 1614.0 182 1 − Metabolite - 2690 1.62 1786.2 441.1 1 + Metabolite - 2691 1.69 1835.8 294.1 1 − Metabolite - 2698 3.88 4500.0 157 1 + Metabolite - 2703 8.86 9054.8 384.1 1 + Metabolite - 2706 10.20 10428.3 247.2 1 + Metabolite - 2711 2.22 2300.0 123 1 + Metabolite - 2724 4.12 4499.3 206.1 1 + Metabolite - 2726 8.30 8854.0 375.2 1 + Metabolite - 2752 2.92 3200.0 189.1 1 + Metabolite - 2753 3.38 3750.0 147 1 + Metabolite - 2766 8.09 8395.0 397 1 + Metabolite - 2768 9.13 9340.0 322.8 1 − Metabolite - 2774 3.53 3796.0 230.9 1 + Metabolite - 2778 7.97 8251.5 376.1 1 + Metabolite - 2781 10.01 10224.6 202.2 1 − Metabolite - 2806 1.38 1491.0 185.1 1 + Metabolite - 2807 8.74 8920.3 380.8 1 + Metabolite - 2809 8.74 8923.5 699.8 1 + Metabolite - 2821 6.80 7680.0 119.1 1 + Metabolite - 2824 12.72 12903.0 773.2 1 + Metabolite - 2827 8.70 8877.0 419.5 1 + Metabolite - 2846 9.19 9369.8 596.6 1 + Metabolite - 2849 - related to 3.17 3375.0 482.6 1 − citric acid? Metabolite - 2853 8.74 8923.5 578.4 1 + Metabolite - 2867 9.65 9908.0 235.3 1 + Metabolite - 2888 - possible 9.87 10153.9 452 1 − sulfated Rosiglitazone Metabolite - 2893 - possible 9.99 10292.8 344.1 1 + demethylated Rosiglitazone Metabolite - 2897 10.96 10100.0 245.2 1 − Metabolite - 2898 11.17 11463.3 213.1 1 − Metabolite - 2900 13.35 13544.7 621.8 1 + Metabolite - 2914 3.75 1096.1 214 Y + Metabolite - 2915 3.77 1099.0 174 Y + Metabolite - 2924 2-hydroxy 4.38 1170.7 130.9 Y + butanoic acid Metabolite - 2973 4.74 1213.4 281 Y + Metabolite - 2974 4.76 1215.6 187 Y + Metabolite - 2978 5.01 1244.1 261.8 Y + Metabolite - 2981 5.21 1265.2 210.9 Y + Metabolite - 3002 6.74 1440.8 296.1 Y + Metabolite - 3003 6.79 1446.6 218.1 Y + Metabolite - 3004 6.81 1449.0 210.9 Y + Metabolite - 3012 7.17 1489.8 232 Y + Metabolite - 3014 - meso 7.43 1520.6 217.1 Y + erythritol Metabolite - 3016 7.58 1537.5 186 Y + Metabolite - 3017 7.61 1541.4 246.1 Y + Metabolite - 3019 7.74 1556.4 260.1 Y + Metabolite - 3020 7.81 1564.1 292 Y + Metabolite - 3022 7.98 1584.9 142 Y + Metabolite - 3023 8.04 1590.9 274.1 Y + Metabolite - 3025 8.11 1600.3 274.1 Y + Metabolite - 3027 8.21 1610.6 142 Y + Metabolite - 3030 8.62 1659.7 320 Y + Metabolite - 3033 8.88 1689.4 116.9 Y + Metabolite - 3034 8.92 1694.9 299 Y + Metabolite - 3040 9.27 1735.7 274.1 Y + Metabolite - 3044 1.52 1615.3 150.1 1 + Metabolite - 3051 8.69 8878.6 835.8 1 + Metabolite - 3053 8.83 9042.0 170.2 1 + Metabolite - 3055 - possible NH3 9.20 9443.0 196.8 1 + adduct of hippuric acid Metabolite - 3056 9.19 9432.0 185.2 1 + Metabolite - 3058 9.70 1786.9 335.1 Y + Metabolite - 3064 13.80 13968.2 516.1 1 + Metabolite - 3067 10.02 1824.2 132 Y + Metabolite - 3073 10.17 1838.8 362.1 Y + Metabolite - 3074 10.22 1844.5 204.1 Y + Metabolite - 3075 10.36 1857.9 204 Y + Metabolite - 3077 10.44 1866.2 308.1 Y + Metabolite - 3078 10.65 1887.0 203.1 Y + Metabolite - 3081 10.89 1911.5 204 Y + Metabolite - 3085 = Inositol 2 11.04 1926.1 217 Y + Metabolite - 3086 11.16 1938.5 221 Y + Metabolite - 3088 11.23 1946.1 372.2 Y + Metabolite - 3089 11.28 1951.5 116.9 Y + Metabolite - 3090 11.31 1955.0 243.1 Y + Metabolite - 3091 11.41 1966.2 232.1 Y + Metabolite - 3093 11.50 1975.6 204 Y + Metabolite - 3094 11.55 1980.6 299 Y + Metabolite - 3097 11.64 1990.4 204 Y + Metabolite - 3098 11.75 2003.0 308 Y + Metabolite - 3099 11.77 2005.2 204 Y + Metabolite - 3101 11.93 2022.2 290 Y + Metabolite - 3102 11.99 2028.2 217.1 Y + Metabolite - 3103 12.09 2039.8 290.1 Y + Metabolite - 3108 12.24 2056.5 246 Y + Metabolite - 3109 12.56 2092.6 202.1 Y + Metabolite - 3113 12.73 2113.5 406.2 Y + Metabolite - 3123 8.97 8763.0 334 1 + Metabolite - 3125 11.88 12095.0 187.1 1 + Metabolite - 3127 8.61 8812.0 260.1 1 − Metabolite - 3129 8.80 9012.0 337.1 1 + Metabolite - 3130 9.09 9328.0 158.2 1 + Metabolite - 3131 10.49 10770.0 192.9 1 + Metabolite - 3132 10.14 10177.0 260.2 1 + Metabolite - 3134 14.33 14487.3 483.1 1 + Metabolite - 3135 14.96 15107.7 467.2 1 + Metabolite - 3138 8.63 8749.0 229.2 1 + Metabolite - 3139 8.82 8934.5 176.1 1 + Metabolite - 3143 9.81 10070.0 160.1 1 + Metabolite - 3146 14.96 15105.0 499.1 1 − Metabolite - 3160 12.11 12247.3 361 1 + Metabolite - 3163 - possible 4.57 4837.5 258 1 + methylcytidine, benserazide, Pyr- Gln-OH, or glycerophosphocholine Metabolite - 3165 8.38 8472.2 265 1 + Metabolite - 3166 8.69 8850.0 394.2 1 + Metabolite - 3167 8.86 8929.0 197.1 1 + Metabolite - 3169 9.27 9384.5 250 1 + Metabolite - 3176 - possible 1.42 1750.0 132 1 + creatine Metabolite - 3178 - possible NH3 3.15 3670.0 210 1 + adduct of isobar 42 Metabolite - 3180 4.14 4500.0 139 1 + Metabolite - 3181 8.59 8621.4 165.1 1 + Metabolite - 3183 - possible 9.37 9441.0 295.2 1 + gamma-L-glutamyl-L- phenylalanine Metabolite - 3184 10.28 10364.4 223 1 + Metabolite - 3189 12.06 12190.0 391 1 + Metabolite - 3215 1.67 1733.8 173.8 1 + Metabolite - 3216 1.68 1743.8 405.7 1 + Metabolite - 3218 2.20 2257.0 148.1 1 + Metabolite - 3220 3.73 4044.1 233.1 1 + Metabolite - 3221 7.97 8050.0 204.1 1 + Metabolite - 3231 3.08 3287.0 104.1 1 + Metabolite - 3238 11.77 11827.4 220 1 + Metabolite - 3243 11.34 11371.0 376.8 1 + Metabolite - 3245 2.14 2168.3 816.7 1 − Metabolite - 3246 - possible Ala- 4.73 5260.0 147.1 1 + GLy, glycyl sarcosine, or ureido- butyric acid Metabolite - 3303 9.51 9799.5 170.1 1 + Metabolite - 3309 8.37 8686.3 512.9 1 + Metabolite - 3311 11.27 11597.0 308.2 1 + Metabolite - 3313 8.10 8529.6 196.9 1 − Metabolite - 3314 8.92 9143.5 264.8 1 + Metabolite - 3317 8.42 8702.3 429.6 1 + Metabolite - 3320 - possible 10.74 11300.0 245 1 − pimpinellin or tetrahydroxybenzophenone Metabolite - 3322 11.82 12044.0 383.2 1 − Metabolite - 3327 11.56 11784.0 385.3 1 − Metabolite - 3364 9.06 9172.1 189 1 − Metabolite - 3365 1.87 2068.3 115.1 1 + Metabolite - 3370 8.11 8529.1 226.2 1 + Metabolite - 3377 8.86 8963.9 270.2 1 + Metabolite - 3379 1.51 1539.0 414.1 1 + Metabolite - 3380 8.26 8602.1 164.1 1 + Metabolite - 3381 2.31 2775.0 335 1 + Metabolite - 3387 9.21 9377.5 463.1 1 + Metabolite - 3390 8.14 8800.0 595.9 1 − Metabolite - 3401 1.73 1863.3 131.1 1 + Metabolite - 3402 8.90 8900.0 343.2 1 + Metabolite - 3409 10.35 10636.4 259.1 1 + Metabolite - 3426 10.71 11051.7 163 1 + Metabolite - 3430 - possible gly- 2.78 3319.7 189.1 1 + leu, acetyl-lys, ala-val Metabolite - 3433 8.41 8681.7 327.1 1 − Metabolite - 3436 8.91 9157.1 157 1 − Metabolite - 3440 9.99 10317.6 252.8 1 − Metabolite - 3441 1.51 1565.4 515 1 + Metabolite - 3443 9.23 9420.6 194.8 1 − Metabolite - 3457 3.81 4193.3 212.9 1 + Metabolite - 3474 15.67 16524.3 228.3 1 + Metabolite - 3475 1.66 1711.9 365.2 1 + Metabolite - 3476 1.65 1709.7 377 1 − Metabolite - 3484 13.59 13710.7 983.4 1 + Metabolite - 3489 3.26 3840.0 226 1 + Metabolite - 3493 9.57 9912.3 335.9 1 + Metabolite - 3498 7.80 8368.7 279.1 1 + Metabolite - 3507 10.01 10631.8 396.2 1 + Metabolite - 3516 10.27 10895.1 411.3 1 + Metabolite - 3517 10.27 10891.5 382.3 1 + Metabolite - 3522 10.38 11005.0 362.3 1 + Metabolite - 3526 10.42 11049.9 404.3 1 + Metabolite - 3531 10.52 11400.0 384.3 1 + Metabolite - 3534 10.54 11174.3 426.3 1 + Metabolite - 3539 10.62 11259.8 435.2 1 + Metabolite - 3543 10.67 11305.2 406.5 1 + Metabolite - 3545 10.69 11331.3 448.4 1 + Metabolite - 3554 10.91 11547.0 521.5 1 + Metabolite - 3564 11.15 11792.0 471.7 1 + Metabolite - 3576 1.38 1539.7 108 1 − Metabolite - 3578 1.36 1525.2 296 1 + Metabolite - 3603 8.41 8971.0 313.6 1 + Metabolite - 3604 8.99 9551.9 214.2 1 − Metabolite - 3605 9.59 10191.4 229.9 1 − Metabolite - 3615 13.61 14343.6 868 1 + Metabolite - 3624 10.36 10984.4 205.1 1 + Metabolite - 3653 - Possible 4.05 4700.0 144.1 1 + stachydrine Metabolite - 3659 10.28 10447.6 427.2 1 + Metabolite - 3660 10.32 10622.4 387.1 1 + Metabolite - 3667 9.17 9120.0 301.1 1 + Metabolite - 3668 9.63 9536.0 379.1 1 + Metabolite - 3670 10.23 10459.0 213 1 − Metabolite - 3694 8.05 8483.7 364.1 1 + Metabolite - 3698 8.31 8640.2 273.1 1 + Metabolite - 3701 1.34 1455.6 141.2 1 + Metabolite - 3706 9.93 9717.0 348 1 + Metabolite - 3707 13.07 13339.5 241 1 + Metabolite - 3708 1.66 1625.3 159.9 1 + Metabolite - 3752 8.61 8750.4 276.1 1 + Metabolite - 3754 9.02 9152.5 190.2 1 + Metabolite - 3755 9.81 9800.0 418.2 1 + Metabolite - 3756 10.02 10319.0 160.9 1 + Metabolite - 3758 12.44 12714.0 309.1 1 − Metabolite - 3761 8.34 8750.0 212 1 + Metabolite - 3765 9.22 9630.0 467.8 1 + Metabolite - 3771 1.68 1761.0 227 1 − Metabolite - 3772 2.22 2274.0 109 1 + Metabolite - 3773 2.26 2275.3 153 1 + Metabolite - 3778 7.37 7200.0 307.3 1 + Metabolite - 3783 1.37 1464.0 271.1 1 + Metabolite - 3786 10.19 9787.3 241.2 1 − Metabolite - 3800 2.00 2400.0 255 1 − Metabolite - 3802 2.18 2200.0 137.1 1 + Metabolite - 3803 2.22 2435.0 198.1 1 + Metabolite - 3804 2.44 2694.5 259 1 + Metabolite - 3805 2.49 2600.0 229.1 1 + Metabolite - 3806 2.80 3155.3 212.1 1 + Metabolite - 3808 3.28 3719.0 288.8 1 − Metabolite - 3810 3.74 4500.0 188.1 1 − Metabolite - 3813 3.81 4312.0 212.1 1 + Metabolite - 3816 4.16 5310.0 173.1 1 − Metabolite - 3817 4.73 4701.0 143.1 1 + Metabolite - 3824 7.88 8197.7 202.1 1 + Metabolite - 3828 8.20 8495.7 245.2 1 + Metabolite - 3830 8.42 8725.0 189 1 − Metabolite - 3832 - possible 8.73 8995.8 173 1 − phenol sulfate Metabolite - 3833 8.81 9100.0 261.1 1 − Metabolite - 3834 - Peptide 9.20 9285.0 372 1 + Metabolite - 3837 9.26 9466.8 212.1 1 − Metabolite - 3841 9.45 9638.0 245.1 1 − Metabolite - 3843 9.54 9721.9 263.1 1 + Metabolite - 3847 9.65 9816.6 206 1 + Metabolite - 3848 9.73 9924.4 192 1 + Metabolite - 3855 9.94 10142.0 243 1 − Metabolite - 3873 9.94 10142.5 219.9 1 + Metabolite - 3876 9.99 10195.0 273 1 − Metabolite - 3877 10.02 10227.0 211 1 − Metabolite - 3878 10.44 10673.0 245 1 + Metabolite - 3879 11.07 11336.5 243 1 − Metabolite - 3886 1.77 1903.3 255 1 − Metabolite - 3887 2.33 2576.0 224.1 1 − Metabolite - 3893 3.26 3724.5 409 1 + Metabolite - 3896 3.38 3868.0 245.2 1 + Metabolite - 3898 3.57 4100.0 194.9 1 + Metabolite - 3900 4.53 4871.7 173.1 1 − Metabolite - 3908 7.98 8301.7 150 1 + Metabolite - 3909 8.21 8497.8 160.1 1 + Metabolite - 3911 8.27 8568.2 116.1 1 + Metabolite - 3951 8.41 8705.4 367.1 1 + Metabolite - 3952 8.70 9150.0 297.2 1 + Metabolite - 3955 8.68 8951.7 357.1 1 − Metabolite - 3957 9.54 9720.8 159.3 1 − Metabolite - 3960 8.49 8744.1 417.1 1 + Metabolite - 3963 10.53 10787.0 652.1 1 + Metabolite - 3966 11.53 11830.0 491.2 1 + Metabolite - 3968 1.39 1436.0 327.8 1 + Metabolite - 3970 4.52 4906.0 226 1 + Metabolite - 3972 6.16 6304.0 432.6 1 − Metabolite - 3973 9.57 9765.0 296.9 1 + Metabolite - 3974 10.12 10349.0 604.1 1 + Metabolite - 3977 11.03 11312.0 187.1 1 − Metabolite - 3980 8.16 8480.4 353.1 1 + Metabolite - 3981 10.01 10234.0 431 1 + Metabolite - 3984 12.76 13134.0 489.1 1 + Metabolite - 3986 13.12 13514.5 489.1 1 + Metabolite - 3992 - possible Cl 1.40 1600.0 129.2 1 − adduct of Formate dimer Metabolite - 3994 1.63 1640.4 427 1 + Metabolite - 3996 5.06 1236.0 176 Y + Metabolite - 3997 2.87 2876.0 564.9 1 − Metabolite - 3998 5.22 1252.7 171 Y + Metabolite - 4002 5.69 1305.2 174 Y + Metabolite - 4003 3.94 4397.0 205 1 + Metabolite - 4010 6.80 1432.8 218.1 Y + Metabolite - 4013 8.05 8399.5 547 1 − Metabolite - 4014 7.17 1474.9 252 Y + Metabolite - 4015 7.37 1498.4 160 Y + Metabolite - 4017 7.62 1527.3 174 Y + Metabolite - 4018 8.35 8589.3 664 1 − Metabolite - 4019 7.68 1534.5 174 Y + Metabolite - 4020 7.91 1561.5 220.1 Y + Metabolite - 4027 8.67 1650.2 274.1 Y + Metabolite - 4030 - possible 11.88 12214.7 218.1 1 + glutethimide or securinine Metabolite - 4031 - possible 14.26 14607.0 244.2 1 + norlevorphenol, isobutylphendienamide, amprolium Metabolite - 4032 8.95 1682.6 156.1 Y + Metabolite - 4042 10.23 1831.9 57.9 Y + Metabolite - 4043 lysine 10.29 1838.6 317.2 Y + Metabolite - 4046 10.80 1890.5 353.1 Y + Metabolite - 4051 11.56 1970.2 357.1 Y + Metabolite - 4053 11.87 2004.6 217.1 Y + Metabolite - 4058 12.46 2070.6 315.1 Y + Metabolite - 4075 13.27 2171.5 103 Y + Metabolite - 4078 16.49 16789.0 663.4 1 + Metabolite - 4080 14.02 2270.2 299 Y + Metabolite - 4084 14.98 2393.9 441.3 Y + Metabolite - 4091 - possible 2.03 2084.7 277 1 + gamma-glutamyl-glutamic acid Metabolite - 4092 5.23 5668.0 256.1 1 + Metabolite - 4096 - possible 8.60 8763.6 318.2 1 + gamma-glu-gly-leu Metabolite - 4112 8.46 8643.5 254.2 1 + Metabolite - 4116 10.26 10582.0 272.2 1 + Metabolite - 4117-possible 14.70 15040.2 260.3 1 + propranolol or 2-heptyl-3- hydroxy-quinolone Metabolite - 4133 4.35 1108.9 198 Y + Metabolite - 4134 5.51 1239.0 60.9 Y + Metabolite - 4147 10.07 1767.1 290.2 Y + Metabolite - 4148 10.23 1786.3 249.2 Y + Metabolite - 4150 11.34 1910.4 306.3 Y + Metabolite - 4163 1.35 1444.1 225.3 1 + Metabolite - 4167 11.03 10920.4 286.2 1 + Metabolite - 4168 13.69 13793.3 686.4 1 + Metabolite - 4196 12.14 2000.4 290.2 Y + Metabolite - 4234 10.57 10467.0 564.4 1 + Metabolite - 4235 10.91 10789.1 652.3 1 + Metabolite - 4238 9.29 9192.0 828.5 1 + Metabolite - 4251 4.09 1130.7 217 Y + Metabolite - 4271 9.69 1777.4 419.2 Y + Metabolite - 4272 10.28 1840.2 669.3 Y + Metabolite - 4274 10.37 1857.0 158.1 Y + Metabolite - 4275 10.68 1887.0 271.1 Y + Metabolite - 4331 13.95 14040.0 679 1 + Metabolite - 4354 3.90 1074.3 110 Y + Metabolite - 4355 6.76 1396.9 102 Y + Metabolite - 4360 9.15 1678.2 347.2 Y + Metabolite - 4361 9.40 1706.2 232.2 Y + Metabolite - 4362 10.02 1779.9 319.2 Y + Metabolite - 4365 11.05 1892.9 204 Y + Metabolite - 4428 7.92 8236.5 229.2 1 + Metabolite - 4448 9.54 9831.4 362.3 1 + Metabolite - 4494 6.45 1363.2 221 Y + Metabolite - 4495 6.59 1381.0 117 Y + Metabolite - 4496 6.76 1398.2 204 Y + Metabolite - 4497 7.05 1431.6 218.1 Y + Metabolite - 4498 7.06 1434.9 103 Y + Metabolite - 4499 7.22 1453.0 189 Y + Metabolite - 4500 7.30 1460.7 172 Y + Metabolite - 4501 imino diacetic 7.96 1538.4 232.1 Y + acid Metabolite - 4502 8.34 1581.3 273.1 Y + Metabolite - 4503 8.39 1589.0 227.2 Y + Metabolite - 4504 8.46 1597.1 244.1 Y + Metabolite - 4505 8.79 1633.4 285 Y + Metabolite - 4507 8.89 1644.9 245 Y + Metabolite - 4509 9.52 1720.6 204 Y + Metabolite - 4510 9.70 1740.1 254 Y + Metabolite - 4511 10.09 1788.4 206 Y + Metabolite - 4512 10.14 1790.7 345.1 Y + Metabolite - 4514 10.31 1812.3 342.2 Y + Metabolite - 4516 11.00 1886.5 217 Y + Metabolite - 4517 11.06 1892.7 217 Y + Metabolite - 4518 11.15 1902.4 295 Y + Metabolite - 4519 11.51 1941.8 193 Y + Metabolite - 4520 11.83 1978.1 325.1 Y + Metabolite - 4521 11.89 1983.4 383.1 Y + Metabolite - 4522 12.26 2025.4 217.1 Y + Metabolite - 4523 12.46 2047.0 258.1 Y + Metabolite - 4524 12.66 2071.3 210.1 Y + Metabolite - 4550 13.25 13286.2 568.2 1 + Metabolite - 4567 3.50 3910.5 203.2 1 + Metabolite - 4593 3.37 1011.1 170.9 Y + Metabolite - 4595 5.65 1274.4 130 Y + Metabolite - 4598 6.69 1392.2 169.9 Y + Metabolite - 4611 8.07 1546.6 292.1 Y + Metabolite - 4615 7.93 8250.0 222.1 1 + Metabolite - 4617 8.39 8588.0 241.3 1 + Metabolite - 4618 8.93 1651.1 349.2 Y + Metabolite - 4620 8.82 9001.0 312.1 1 + Metabolite - 4624 10.01 1779.1 342.2 Y + Metabolite - 4628 10.11 1786.4 267.1 Y + Metabolite - 4629 10.29 1806.9 274.1 Y + Metabolite - 4632 10.59 1840.6 166 Y + Metabolite - 4633 10.69 1851.2 129 Y + Metabolite - 4634 11.00 1884.3 333.1 Y + Metabolite - 4635 11.19 1908.7 192.9 Y + Metabolite - 4636 11.50 1937.7 483.3 Y + Metabolite - 4637 11.95 1988.1 193 Y + Metabolite - 4638 12.25 2021.4 203.1 Y + Metabolite - 4639 12.87 2092.4 156.1 Y + Metabolite - 4649 5.33 5997.0 164.1 1 + Metabolite - 4667 5.36 5652.8 320.1 1 − Metabolite - 4706 8.92 9069.8 219 1 − Metabolite - 4767 8.77 1626.2 116.9 Y + Metabolite - 4768 9.04 1661.7 279.1 Y + Metabolite - 4769 11.30 1916.4 156 Y + Metabolite - 4787 11.13 10895.5 289.4 1 + Metabolite - 4791 10.29 1796.4 366.4 Y + Metabolite - 4795 14.83 2350.3 309 Y + Metabolite - 4796 3.53 1043.6 117 Y + Metabolite - 4806 4.20 1122.8 104.9 Y + Metabolite - 4866 9.18 9069.0 506.7 1 + Metabolite - 4868 - confirmed 9.44 9356.0 531.2 1 + Bradykinin Metabolite - 4869 10.25 10112.8 534.5 1 + Metabolite - 4931 1.50 1659.5 431 1 + Metabolite - 4986 11.56 1956.4 204.1 Y + Metabolite - 5086 9.51 9738.3 388.2 1 + Metabolite - 5087 9.69 9924.9 432.3 1 + Metabolite - 5089 9.85 10075.9 476.3 1 + Metabolite - 5107 11.87 11986.0 516.7 1 + Metabolite - 5108 12.00 12116.5 538.7 1 + Metabolite - 5109 12.12 12248.5 560.7 1 + Metabolite - 5110 12.24 12350.5 582.6 1 + Metabolite - 5126 9.78 10017.0 358.3 1 + Metabolite - 5128 3.12 3462.8 558 1 − Metabolite - 5147 8.21 8508.0 262.2 1 + Metabolite - 5166 12.90 12999.4 491.5 1 + Metabolite - 5167 12.97 13070.0 506.2 1 + Metabolite - 5170 8.93 9156.3 279.1 1 + Metabolite - 5186 1.55 1709.7 163.9 1 + Metabolite - 5187 3.53 3985.5 489.1 1 + Metabolite - 5189 16.33 16650.0 528.2 1 + Metabolite - 5207 7.41 1493.6 151 Y + Metabolite - 5209 8.10 1573.6 218.2 Y + Metabolite - 5210 8.47 1616.4 254.1 Y + Metabolite - 5211 8.77 1652.1 326.2 Y + Metabolite - 5212 8.88 1665.1 306.1 Y + Metabolite - 5213 8.97 1675.3 111.1 Y + Metabolite - 5214 11.54 1960.0 117 Y + Metabolite - 5215 11.98 2008.0 163 Y + Metabolite - 5226 3.73 1073.9 102 Y + Metabolite - 5227 6.59 1398.6 151 Y + Metabolite - 5228 6.97 1442.5 181.1 Y + Metabolite - 5229 7.13 1461.6 211.1 Y + Metabolite - 5232 12.19 2031.5 134 Y + Metabolite - 5346 8.33 1573.0 202 Y + Metabolite - 5349 10.10 1782.2 312.1 Y + Metabolite - 5366 12.49 2044.7 204 Y + Metabolite - 5403 5.92 1300.2 319 Y + Metabolite - 5419 9.05 1664.1 349.2 Y + Metabolite - 5427 10.67 1853.0 192.9 Y + Metabolite - 5437 12.17 2017.4 204 Y + Metabolite - 5489 8.10 1550.3 247.1 Y + Metabolite - 5847 12.35 2040.0 288.2 Y + Metabolite - 5906 7.82 1541.2 313.9 Y + Metabolite - 5907 8.69 1643.2 229.1 Y +

While the invention has been described in detail and with reference to specific embodiments thereof, it will be apparent to one skilled in the art that various changes and modifications can be made without departing from the spirit and scope of the invention. 

What is claimed is:
 1. A method of distinguishing low grade prostate cancer from high grade prostate cancer in a subject having prostate cancer, comprising: analyzing a biological sample from a subject to determine the level(s) of one or more biomarkers for low grade prostate cancer and/or high grade prostate cancer in the sample, wherein the one or more biomarkers are selected from Tables 3, 8, 11, 20, and/or 26 and comparing the level(s) of the one or more biomarkers in the sample to low grade prostate cancer-positive reference levels that distinguish over high grade prostate cancer and/or to high grade prostate cancer-positive reference levels that distinguish over low grade prostate cancer in order to determine whether the subject has low grade or high grade prostate cancer.
 2. The method of claim 1, wherein the biological sample is prostate tissue and the one or more biomarkers are selected from Table
 3. 3. The method of claim 1, wherein the biological sample is blood plasma and the one or more biomarkers are selected from Table 8 and/or
 26. 4. The method of claim 1, wherein the biological sample is urine and the one or more biomarkers are selected from Table 11 and/or
 20. 5. The method of claim 1, wherein the one or more biomarkers are selected from those biomarkers in Tables 3, 8, 11, 20, and/or 26 having p values of less than 0.05 and/or those biomarkers in Tables 3, 8, 11, 20, and/or 26 having q values of less than 0.10.
 6. The method of claim 1, wherein the one or more biomarkers comprise one or more biomarkers selected from the group consisting of sarcosine, citric acid, spermine, and malic acid.
 7. The method of claim 1, wherein the comparing step comprises comparing a ratio of the levels of one or more biomarkers in Tables 1, 2, 4, 5, 6, 7, 9, 10, 13, 15, 18, 22, and/or 24 having p values of less than 0.05 and/or those biomarkers in Tables 1, 2, 4, 5, 6, 7, 9, 10, 13, 15, 18, 22, and/or 24 having q values of less than 0.10 to low grade prostate cancer-positive reference levels that distinguish over high grade prostate cancer and/or high grade prostate cancer-positive reference levels that distinguish over low grade prostate cancer in order to determine whether the subject has low grade or high grade prostate cancer.
 8. The method of claim 7, wherein the ratio of the levels of one or more biomarkers comprises the level of a biomarker selected from the group consisting of sarcosine, spermine, and malic acid.
 9. The method of claim 1, wherein the comparing step comprises comparing a ratio of the levels of one or more biomarkers to low grade prostate cancer-positive reference levels that distinguish over high grade prostate cancer and/or high grade prostate cancer-positive reference levels that distinguish over low grade prostate cancer in order to determine whether the subject has low grade or high grade prostate cancer, wherein the ratio is selected from the group consisting of the ratio of levels of sarcosine and citric acid, sarcosine and spermine, and citric acid and malic acid.
 10. The method of claim 1, wherein the sample is analyzed using one or more techniques selected from the group consisting of gas chromatography, liquid chromatography, mass spectrometry, ELISA, and antibody linkage.
 11. A method of monitoring progression/regression of prostate cancer in a subject comprising: analyzing a first biological sample from a subject to determine the level(s) of one or more biomarkers for prostate cancer in the sample, wherein the one or more biomarkers are selected from Tables 1, 2, 3, 4, 5, 6, 7, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26 and the first sample is obtained from the subject at a first time point; analyzing a second biological sample from a subject to determine the level(s) of the one or more biomarkers, wherein the second sample is obtained from the subject at a second time point; and comparing the level(s) of one or more biomarkers in the first sample to the level(s) of the one or more biomarkers in the second sample in order to monitor the progression/regression of prostate cancer in the subject.
 12. The method of claim 11, wherein the method further comprises comparing the level(s) of one or more biomarkers in the first sample, the level(s) of one or more biomarkers in the second sample, and/or the results of the comparison of the level(s) of the one or more biomarkers in the first and second samples to prostate cancer-positive and/or prostate cancer-negative reference levels of the one or more biomarkers.
 13. The method of claim 11, wherein the sample is analyzed using one or more techniques selected from the group consisting of gas chromatography, liquid chromatography, mass spectrometry, ELISA, and antibody linkage.
 14. A method of assessing the efficacy of a composition for treating prostate cancer comprising: analyzing, from a subject having prostate cancer and currently or previously being treated with a composition, a biological sample to determine the level(s) of one or more biomarkers for prostate cancer selected from Tables 1, 2, 4, 5, 6, 7, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26; and comparing the level(s) of the one or more biomarkers in the sample to (a) levels of the one or more biomarkers in a previously-taken biological sample from the subject, wherein the previously-taken biological sample was obtained from the subject before being treated with the composition, (b) prostate cancer-positive reference levels of the one or more biomarkers, and/or (c) prostate cancer-negative reference levels of the one or more biomarkers.
 15. The method of claim 14, wherein the sample is analyzed using one or more techniques selected from the group consisting of gas chromatography, liquid chromatography, mass spectrometry, ELISA, and antibody linkage.
 16. A method for assessing the efficacy of a composition in treating prostate cancer, comprising: analyzing a first biological sample from a subject to determine the level(s) of one or more biomarkers for prostate cancer selected from Tables 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26, the first sample obtained from the subject at a first time point; administering the composition to the subject; analyzing a second biological sample from the subject to determine the level(s) of the one or more biomarkers, the second sample obtained from the subject at a second time point after administration of the composition; comparing the level(s) of one or more biomarkers in the first sample to the level(s) of the one or more biomarkers in the second sample in order to assess the efficacy of the composition for treating prostate cancer.
 17. The method of claim 16, wherein the sample is analyzed using one or more techniques selected from the group consisting of gas chromatography, liquid chromatography, mass spectrometry, ELISA, and antibody linkage.
 18. A method of assessing the relative efficacy of two or more compositions for treating prostate cancer comprising: analyzing, from a first subject having prostate cancer and currently or previously being treated with a first composition, a first biological sample to determine the level(s) of one or more biomarkers selected from Tables 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 15, 18, 20, 22, 24, and/or 26; analyzing, from a second subject having prostate cancer and currently or previously being treated with a second composition, a second biological sample to determine the level(s) of the one or more biomarkers; and comparing the level(s) of one or more biomarkers in the first sample to the level(s) of the one or more biomarkers in the second sample in order to assess the relative efficacy of the first and second compositions for treating prostate cancer.
 19. The method of claim 18, wherein the sample is analyzed using one or more techniques selected from the group consisting of gas chromatography, liquid chromatography, mass spectrometry, ELISA, and antibody linkage. 